Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encodi...Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical(IHC)analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.展开更多
目的:检索并总结下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的相关证据,为下肢动脉硬化闭塞症伴间歇性跛行病人进行运动锻炼提供参考。方法:根据“6S”金字塔证据模型,系统检索BMJ Best Practice、UpToDate、the Cochrane Library、...目的:检索并总结下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的相关证据,为下肢动脉硬化闭塞症伴间歇性跛行病人进行运动锻炼提供参考。方法:根据“6S”金字塔证据模型,系统检索BMJ Best Practice、UpToDate、the Cochrane Library、澳大利亚Joanna Briggs Institute(JBI)循证卫生保健中心、英国国家卫生和临床优化研究所(NICE)、苏格兰校际指南网(SIGN)、美国国立指南库(NGC)、医脉通、美国血管外科协会、美国心脏病学会(ACC)、美国心脏协会(AHA)、欧洲心脏协会、PubMed、CINANL、MedLine、Web of Science、中国生物医学文献数据库、中国知网、万方数据库和维普数据库公开发表的下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的相关文献,包括临床实践指南、专家共识、证据总结、系统评价和Meta分析,检索时限从2017年10月—2023年10月。经过质量评价并结合专业判断,对纳入的文献进行证据的提取和汇总。结果:共纳入25篇文献,包括2篇临床实践指南、4篇证据总结、1篇专家共识、18篇系统评价,最终从运动的适用对象、运动的评估/培训、运动地点、运动流程、运动类型/方式、运动时间/频次/周期、运动强度、运动时的质量控制、运动的效果评价、运动时的注意事项10个方面形成下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的最佳证据。结论:本研究总结了下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的最佳证据,为制定下肢动脉硬化闭塞症伴间歇性跛行病人的运动方案提供证据支持。展开更多
目的探讨钆塞酸二钠多期动态增强MRI对慢性乙型肝炎肝纤维化分期的定量评估价值。方法纳入行3.0 T MR检查并证实为肝纤维化的慢性乙型肝炎患者共86例,其中S1期25例、S2期18例、S3期23例、S4期20例。所有患者均行MRI平扫及钆塞酸二钠增...目的探讨钆塞酸二钠多期动态增强MRI对慢性乙型肝炎肝纤维化分期的定量评估价值。方法纳入行3.0 T MR检查并证实为肝纤维化的慢性乙型肝炎患者共86例,其中S1期25例、S2期18例、S3期23例、S4期20例。所有患者均行MRI平扫及钆塞酸二钠增强扫描,测量平扫、动脉期、门脉期、移行期及20 min肝胆期肝实质的信号强度(Signal Intensity,SI)值,计算动脉期、门脉期、移行期及肝胆期肝实质的相对强化率(REAP、REPV、RETP、REHBP)和对比增强指数(CEIAP、CEIPV、CEITP、CEIHBP)。采用Kruskal-Wallis H检验定量评估增强后四期相对强化率和对比增强指数对肝纤维化S1~S4分期的价值,并使用ROC曲线评估多期增强定量参数在诊断≥S2期、≥S3期、S4期肝纤维化中的诊断效能。结果在不同肝纤维化分期(S1~S4)的相对强化率差异有统计学意义,且随着肝纤维化分期的升高,REPV、RETP、CEITP、CEIHBP值依次降低,CEIHBP诊断≥S2期、≥S3期、S4期不同肝纤维化分期的效能较RETP、CEITP高,AUC分别为0.775(95%CI:0.672~0.858)、0.765(95%CI:0.662~0.850)、0.821(95%CI:0.723~0.895),REPV诊断效能最低。结论钆塞酸二钠多期动态增强后肝实质RETP、CEITP、CEIHBP在定量评估肝纤维化分期方面有一定的诊断价值,以CEIHBP诊断效能最佳。展开更多
基金National Key Research and Development Program of China,Grant/Award Number:2021YFC2301403 and 2022YFF0711000。
文摘Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical(IHC)analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.
文摘目的:检索并总结下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的相关证据,为下肢动脉硬化闭塞症伴间歇性跛行病人进行运动锻炼提供参考。方法:根据“6S”金字塔证据模型,系统检索BMJ Best Practice、UpToDate、the Cochrane Library、澳大利亚Joanna Briggs Institute(JBI)循证卫生保健中心、英国国家卫生和临床优化研究所(NICE)、苏格兰校际指南网(SIGN)、美国国立指南库(NGC)、医脉通、美国血管外科协会、美国心脏病学会(ACC)、美国心脏协会(AHA)、欧洲心脏协会、PubMed、CINANL、MedLine、Web of Science、中国生物医学文献数据库、中国知网、万方数据库和维普数据库公开发表的下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的相关文献,包括临床实践指南、专家共识、证据总结、系统评价和Meta分析,检索时限从2017年10月—2023年10月。经过质量评价并结合专业判断,对纳入的文献进行证据的提取和汇总。结果:共纳入25篇文献,包括2篇临床实践指南、4篇证据总结、1篇专家共识、18篇系统评价,最终从运动的适用对象、运动的评估/培训、运动地点、运动流程、运动类型/方式、运动时间/频次/周期、运动强度、运动时的质量控制、运动的效果评价、运动时的注意事项10个方面形成下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的最佳证据。结论:本研究总结了下肢动脉硬化闭塞症伴间歇性跛行病人运动疗法的最佳证据,为制定下肢动脉硬化闭塞症伴间歇性跛行病人的运动方案提供证据支持。
