Four polysaccharides(MCPa,MCPb,MCPc,MCPd)were obtained from Lepidium meyenii Walp.Their structures were characterized by chemical and instrumental methods including total sugar,uronic acid and protein content determi-...Four polysaccharides(MCPa,MCPb,MCPc,MCPd)were obtained from Lepidium meyenii Walp.Their structures were characterized by chemical and instrumental methods including total sugar,uronic acid and protein content determi-nation,UV,IR and NMR spectroscopy,as well as monosaccharide composition determination and methylation analy-ses.Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa,and shared a similar backbone chain consisting of(1→4)-glucose linkages with branches attached to C-3 and C-6.Furthermore,bioactivity assay showed that MCPs had concentration-dependent inhibitory activity onα-glucosidase.MCPb(Mw=10.1 kDa)and MCPc(Mw=5.62 kDa)with moderate molecular weights exhibited higher inhibitory activ-ity compared with MCPa and MCPd.展开更多
Ubiquitously distributed microorganisms are natural decomposers of environmental pollutants.However,because of continuous generation of novel recalcitrant pollutants due to human activities,it is difficult,if not impo...Ubiquitously distributed microorganisms are natural decomposers of environmental pollutants.However,because of continuous generation of novel recalcitrant pollutants due to human activities,it is difficult,if not impossible,for microbes to acquire novel degradation mechanisms through natural evolution.Synthetic biology provides tools to engineer,transform or even re-synthesize an organism purposefully,accelerating transition from unable to able,inefficient to efficient degradation of given pollutants,and therefore,providing new solutions for environmental bioremediation.In this review,we described the pipeline to build chassis cells for the treatment of aromatic pollutants,and presented a proposal to design microbes with emphasis on the strategies applied to modify the target organism at different level.Finally,we discussed challenges and opportunities for future research in this field.展开更多
基金the National Natural Science Foundation of China(No.31872675)the Cooperation Project with DR PLANT Company(2023).
文摘Four polysaccharides(MCPa,MCPb,MCPc,MCPd)were obtained from Lepidium meyenii Walp.Their structures were characterized by chemical and instrumental methods including total sugar,uronic acid and protein content determi-nation,UV,IR and NMR spectroscopy,as well as monosaccharide composition determination and methylation analy-ses.Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa,and shared a similar backbone chain consisting of(1→4)-glucose linkages with branches attached to C-3 and C-6.Furthermore,bioactivity assay showed that MCPs had concentration-dependent inhibitory activity onα-glucosidase.MCPb(Mw=10.1 kDa)and MCPc(Mw=5.62 kDa)with moderate molecular weights exhibited higher inhibitory activ-ity compared with MCPa and MCPd.
基金supported by grants from the National Natural Science Foundation of China(31971347,32030004 and 31725002)Shenzhen Science and Technology Program(KQTD20180413181837372)+1 种基金Guangdong Provincial Key Laboratory of Synthetic Genomics(2019B030301006)Shenzhen Outstanding Talents Training Fund.
文摘Ubiquitously distributed microorganisms are natural decomposers of environmental pollutants.However,because of continuous generation of novel recalcitrant pollutants due to human activities,it is difficult,if not impossible,for microbes to acquire novel degradation mechanisms through natural evolution.Synthetic biology provides tools to engineer,transform or even re-synthesize an organism purposefully,accelerating transition from unable to able,inefficient to efficient degradation of given pollutants,and therefore,providing new solutions for environmental bioremediation.In this review,we described the pipeline to build chassis cells for the treatment of aromatic pollutants,and presented a proposal to design microbes with emphasis on the strategies applied to modify the target organism at different level.Finally,we discussed challenges and opportunities for future research in this field.
