The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein...The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein expression. With the application of reporter genes, such as <i>gus</i>A (<i>uid</i>A,) the production of a colored protein, <i>β</i>-glucuronidase, can be detected and measured both qualitatively and quantitatively, and the activity of the promoter can be assessed. In this work we use a promoter of an abundant banana fruit protein gene <i>Musa acuminata</i> Acidic Chitinase class III a monocot species, to drive expression of <i>gus</i>A in a dicot species, like tomato. We evaluated the monocot promoter capabilities by localizing and quantifying <i>β</i>-glucuronidase (GUS) expression through fluorometric assays during tomato fruit ripening. Our results suggest that this promoter could be used for specifically strong fruit protein expression in dicot plants.展开更多
文摘The knowledge generated from the identification of plant promoters has been very important for plant biotechnology development. The use of promoters in transgenic plants allows a reasonable level of regulating protein expression. With the application of reporter genes, such as <i>gus</i>A (<i>uid</i>A,) the production of a colored protein, <i>β</i>-glucuronidase, can be detected and measured both qualitatively and quantitatively, and the activity of the promoter can be assessed. In this work we use a promoter of an abundant banana fruit protein gene <i>Musa acuminata</i> Acidic Chitinase class III a monocot species, to drive expression of <i>gus</i>A in a dicot species, like tomato. We evaluated the monocot promoter capabilities by localizing and quantifying <i>β</i>-glucuronidase (GUS) expression through fluorometric assays during tomato fruit ripening. Our results suggest that this promoter could be used for specifically strong fruit protein expression in dicot plants.
