Effect of Astragalus-hawthorn on ovarian reproductive function and inflammatory mechanism of action in rats with polycystic ovary syndrome

Objective:To investigate the effects of prophylactic administration of Astragalus-Hawthorn on ovarian reproductive function and inflammatory mechanism in rats with polycystic ovary syndrome(PCOS).Methods:Fifty 21-day-old female rats were randomly divided into five groups of 10 rats each:Normal group,Model group,Astragalus group,Hawthorn group and Astragalus-Hawthorn group.All groups,except the normal group,were fed a high-fat model diet.Each treatment group received astragalus,hawthorn and an astragalus-hawthorn solution orally during the molding period for an intervention period of 15 weeks.The estrous cycle of the rats in each group was observed under the microscope from week 8,the interference rate was calculated,changes in ovarian tissues were observed by HE staining and the levels of sex hormones,proinflammatory and anti-inflammatory factors in the serum of the rats were measured by ELISA.Results:Rate of estrous cycle disorders were more frequent in the model group than in the normal group.(P<0.01);ovarian tissue was polycystic,with few corpora lutea and sinusoids and numerous follicular ovarian cysts;Luteinising hormone/follicle-stimulating hormone(LH/FSH),testosterone(T),interleukin 6(IL-6),interleukin 17A(IL-17A)and tumour necrosis factor(TNF-α)levels were significantly higher in the model group than in the normal group;interleukin 10(IL-10)was significantly lower than in the normal group(P<0.05).Compared to the model group,rats in the Astragalus and hawthorn groups had a lower rate of estrous cycle disorders(P<0.01);the number of cystic follicles in the ovarian tissue decreased;the number of mature follicles and corpus luteum increased;LH/FSH,T,IL-6 and IL-17A levels were significantly lower in the Astragalus group,hawthorn group and the Astragalus and hawthorn groups;TNF-αlevels were significantly lower in the astragalus and hawthorn groups;IL-10 levels were significantly higher in the hawthorn and astragalus hawthorn groups(P<0.05).IL-10 levels were significantly higher in the astragalus and hawthorn groups(P<0.05).Compared to the astragalus group,T levels were significantly lower in the astragalus and hawthorn groups and IL-10 levels were significantly higher in the astragalus and hawthorn groups(P<0.05).Compared to the hawthorn group,levels of T and IL-17A were significantly lower in the astragalus-hawthorn group(P<0.05).Conclusion:Astragalus-Hawthorn prophylactic administration can improve ovarian reproductive function in rats with PCOS,and the mechanism of action may be related to reducing the levels of pro-inflammatory factors IL-6,IL-17A and TNF-α,increasing the levels of anti-inflammatory factor IL-10,and reducing the inflammatory state.

黄芪-山楂对多囊卵巢综合征大鼠卵巢生殖功能的影响及炎性作用机制

目的:探讨黄芪-山楂预防给药对于多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠卵巢生殖功能的的影响及炎性作用机制。方法:将50只21日龄雌性大鼠随机按10只一组分为5组,分别为正常组、模型组、黄芪组、山楂组、黄芪-山楂组。除正常组外,其余各组均喂食高脂饲料来造模。各给药组在造模期间分别以黄芪、山楂、黄芪-山楂水溶液灌胃,持续干预15周。第8周开始镜下观察各组大鼠动情周期并计算紊乱占比;HE染色法观察卵巢的组织变化;采用ELISA测定大鼠血清中性激素、促炎因子和抗炎因子的水平。结果:与正常组比较,模型组大鼠出现动情周期紊乱占比升高(P<0.01);卵巢组织多囊改变,可见较少数量的黄体和具有许多滤泡卵巢囊肿的窦卵泡;促黄体生成素/卵泡刺激素(LH/FSH)、睾酮(T)、白介素6(IL-6)、白介素17A(IL-17A)、肿瘤坏死因子(TNF-α)水平显著升高;白介素10(IL-10)水平显著降低(P<0.05)。与模型组比较,黄芪-山楂组大鼠出现动情周期紊乱占比降低(P<0.01);卵巢组织中的囊性卵泡数减少,成熟卵泡和黄体数增多;黄芪组、山楂组、黄芪-山楂组LH/FSH、T、IL-6,IL-17A水平显著降低;黄芪组、黄芪-山楂组TNF-α水平显著降低;山楂组、黄芪-山楂组IL-10水平显著升高(P<0.05)。与黄芪组比较,黄芪-山楂组T水平显著降低;IL-10水平显著上升(P<0.05)。与山楂组比较,黄芪-山楂组T、IL-17A水平显著降低(P<0.05)。结论:黄芪-山楂预防给药可以改善PCOS大鼠的卵巢生殖功能,其作用机制可能与降低促炎因子IL-6、IL-17A、TNF-α水平,提高抗炎因子IL-10水平,减轻炎症状态有关。

水萃取法测定能力验证样品中甲醛含量的不确定度评定

为有效提高甲醛含量的检测工作质量,对能力验证样品中甲醛含量的测定进行不确定评定,分析测量结果各不确定分量对不确定总量的贡献,确定主要影响因素。结果显示,本次测量不确定度主要来源依次为重复性测定、萃取液体积及分析仪器。因此,试验过程中操作人员应熟知相关标准要求,掌握相关操作技术,从而提高试验的精密度和准确度。