益肺汤调控TGF-β1/Smad2通路抗肺纤维化机制研究

目的:研究益肺汤含药血清对血管紧张素Ⅱ(AngⅡ)诱导的肺成纤维细胞(NIH-3T3)的影响,探讨其抑制肺纤维化的作用机制。方法:采用SPF级C57小鼠灌胃益肺汤,1次/d,连续给药3 d制备含药血清。实验分为正常对照组、模型组、模型+正常血清组、模型+含药血清组,给药48 h后0.1%FBS的培养基饥饿处理24 h,100 ng/μl的AngⅡ诱导造模24 h。免疫组化检测α-平滑肌肌动蛋白(α-SMA);免疫荧光检测Smad2;Western blot检测Ⅰ型胶原蛋白(ColⅠ)、α-SMA、纤维连接蛋白(FN)、Smad2;ELISA检测细胞上清转化生长因子-β1(TGF-β1)的表达情况。结果:Western blot和免疫荧光显示,造模后,α-SMA、FN、ColⅠ、Smad2的蛋白相对表达显著升高,差异具有统计学意义(均P<0.05)。益肺汤含药血清干预48 h后造模,α-SMA、FN、Smad2、ColⅠ的相对量较模型组下降(均P<0.05)。ELISA结果显示,造模后,TGF-β1的相对表达显著升高(P<0.05),益肺汤含药血清干预48 h后,TGF-β1相对量较模型组下降(P<0.05)。结论:益肺汤含药血清对AngⅡ诱导的NIH-3T3有一定影响,可有效减轻肺纤维化,其作用机制可能与调控TGF-β1/Smad2信号通路,降低α-SMA、ColⅠ、FN的表达有关。

4-17 Investigations on Interaction between Low Energy Heavy Ion Beams and Hydrogen Plasma

The interaction between ion beam and matter is one of the most important topics in atomic physics and nuclear physics. It is indeed a requirement for a deeper understanding of the interaction processes. Especially the energy deposition by an intense heavy ion beam with the low energy impinging into a degenerate matter, which is related to the topics of warm dense matter, fast ignition process and helium ions self-heating in the fusion process.

2-4 First Isochronous Mass Measurement in Neutron-rich Region at CSRe

Mass is one of the fundamental properties of atomic nuclei. Isochronous mass spectrometry (IMS), using astorage ring combined with an in-flight separator, has been shown to be a powerful tool for mass measurementof exotic nuclei[1]. Recently, masses of many proton-rich nuclides were accurately determined at the HIRFL-CSRfacility[2]. In this paper, we described the first isochronous mass measurement of neutron-rich nuclides at CSRe.This experiment was performed at the end of 2011. In the experiment, the primary beam of 86Kr28+ ions wasaccumulated and accelerated to an energy of 460.65 MeV/u in the synchrotron CSRm. The 86Kr28+ ions were fastextracted and focused on a 15 mm thick beryllium target which was placed at the entrance of the RIBLL2 (anin-flight fragment separator).

2-6 First Isochronous Mass Measurements with Two Time-of-Flight Detectors at CSRe

In conventional isochronous mass spectrometry (IMS), single time-of-flight (TOF) method is adopted to measurethe ions' revolution times in a storage ring which can then be used to calculate the ions' masses. However, themass-to-charge ratio (m=q) is only related to the revolution time (T) under the condition that is equal to taccording to the following equation:

2-7 Charge and Frequency Resolved Isochronous Mass Spectrometry in Storage Rings: First Direct Mass Measurement of the Short-lived Neutron-de cient 51Co Nuclide

Isochronous mass spectrometry in storage rings is a successful technique for the precision mass measurements ofthe nuclides with half-lives down to tens of microseconds[1]. Since the isochronous condition =t greatly reducesthe influence of the velocity difference on the ion revolution periods, the revolution period difference ΔT =T ??TRof a stored ion with respect to a reference time TR is directly related to its mass-to-charge ratio difference Δ(m=q),written in the first order as:

4-13 Velocity Map Imaging Spectrometer for Photon-fullerene Collision Experiments

Imaging detector is an increasingly popular tool for measuring kinetic energy distributions of photoelectrons orions from photoionization and photodissociation[1;2]. An important advance in imaging is Velocity Map Imaging(VMI)[3], where the electron/ion optics consists of a lens, which signicantly reduces the sensitivity of the positionof impact on detector on the position of creation of the ion or electron, while maintaining the dependence of theposition of impact on the initial velocity.

4-4 Ultracold Plasma: An Experimental Realization

Ultracold plasma has well defined initial conditions, including the particle densities of more than 105 cm??3 as well as the electron temperature in the range of 11 000 K and ion temperature from tens of millikelvin to a few Kelvin. So the ultracold plasma provides an ideal environment for the study of strongly coupled systems, where the Coulomb interaction is comparable with the thermal kinetic energy.

Benchmark nℓ-resolved Cross Sections of Single and Double Charge Exchange Processes in Slow C^(4+)+He Collisions

X-ray and extreme-ultraviolet(EUV)emissions have been detected from many comets.It is now recognized that the charge exchange(CX)mechanism between highly charged solar wind ions and cometary neutrals is responsible for the observed emissions.State-resolved CX cross sections are of the utmost importance for modeling related photon emissions.

Plasma Wake-field Modi cation Onproton Beams Spot

We newly completed the experimental setup for ion-plasma interaction at 320 kV ECR platform,a long RF-plasma target,1.2 Tm bending magnet and a specially designed time-resolved position sensitive detector system were placed along the beam line(Fig.1).

Study of Energy Loss of Proton Beams in Hydrogen Plasmas

The energy loss of charged partiles in matter has been studied intensively both theoretically and experimentally[1-3].The energy loss of ion in ionized matter is extremely important for the development of inertial confinement fusion target.Therefore,a detailed understanding of the interaction mechanism is necessary.

Photoion Velocity Map Imaging in Magneto-optical Trap

Ultracold plasmas(UCPs),created via photoionizing laser-cooling atoms in magneto-optical trap(MOT)[1],have the temperature of ions around 1 K and the temperature of electrons ranging from 1 to 1000 K.Due to the low temperature of the ions,the ultracold plasma is considered to be strongly coupled,in which the Coulomb effect dominants the evolution of plasmas.Here we present an idea and a test to measure the temperature of ions in UCPs[2]using a novel method of velocity map imaging(VMI).

Charge State and Wake-field Modification of Ions in Plasma

Plasma,called the fourth form of matter,not only plays an important role in astrophysics,but also becomes the new interest in atomic physics,material science and other aspects.Ion beam–plasma interaction can be a new solution to understand the plasma properties.For instance the collision of ion and free electrons dominate in plasma,which can induce the higher energy loss and the change of charge state,as well as the wake-field can modify the ion beam from a continues mode to a pulsed mode with a focused/unfocused spot.

Measuring the Collective Space-charge Effect in Low-density Ion Beams Using Velocity Map Imaging Spectrometer

Understanding weak space-charge effects(SCEs)is central for the development of the velocity map imaging(VMI)technique for studying the structure,thermodynamics,and dynamics of molecules by imaging photoions and(or)photoelectrons[1,2].However,to our knowledge,it is very difficult to determine the SCEs existing at low-density ion beams due to the limited precision of the present measurement methods.Here,we present a method to directly characterize the weak SCEs employing the high-resolution velocity map imaging spectrometer.The delay ionization of C60 molecules,induced by a nanosecond laser beam,was used to produce ion beams with low density.

Study on the Stability of Laser-induced Breakdown Spectroscopy from Microelements in Complex Granular Matters

Taking the edible salt as a model sample of complex granular matters,the stability of laser-induced breakdown spectroscopy(LIBS)from microelements in complex granular matters has been studied.We measured the intensities of emission spectral lines from four typical microelements(Ca,Sr,Mg,and Fe)in the sample at different experimental conditions ranging from 250 nn to 465 nm(see Fig.1).We extracted the relative standard deviation(RSD)and signal to ratio of each spectral line as functions of the laser-focus position to the surface of sample,the number of laser pulse to accumulate a spectrum,and the delayed time to record the spectral lines after laser pulse.This leads us to determine the most optimal conditions for synchronously detecting the four microelements.

New Progress on Ion-plasma Interaction and the Developing Diagnostics Technology for HEDM at HIAF

Plasma as the fourth state of matter widely exists in universe and it is the essential state in the process of Initial Confinement Fusion sciences.Ion beam is a unique tool to understand some special properties of plasma itself form the aspect of kinetic interaction between ion and plasma surrounding.The energy loss of helium ions in a dense plasma relates to the self-heating process in a ICF capsule and the radiation protection by Van Allen Radiation Belt of Earth-self against the solar wind.It is a very interesting topic to carry out the series of experiments applying the ion beam and plasma target together in lab[1,2].

Detection Efficiency Test of Ion Velocity Map Imaging Spectrometer

In recent years,ultracold neutral plasmas(UNPs),as strongly coupled plasma available in the laboratory,have gradually been the focus in the experimental and theoretical plasma physics studies[1].However,measuring the temperature of UNPs is always a challenge in the experiments.Here,we propose to obtain the kinetic energy distribution of charged particles in UNPs using the ion velocity map imaging(VMI)technique.And then,the temperature of UNPs,as well as the Coulomb coupling parameter,can also be obtained.

3-24 Energy Loss of Protons Beam with Different Energies in Hydrogen Plasma

The interaction of ion beam with matter has always been a classical point of atomic and nuclear physics[1].However there still need deeper understanding of physical processes in theory.In this paper the energy loss of protons beam with different energies in hydrogen plasma target was introduced and compared with the theoretical predictions by applying the Bethe theory.

3-10 State-selective Spontaneous Evolution of Rydberg Atoms into an Ultracold Plasma

We measured the state distribution of cold Rydberg atoms for various initially excited Rydberg levels and evolution times in order to investigate the collision-induced dynamics.The cold Rydberg atoms were excited into nP states(n=20,25,30,…,97)below the ionization potential.Immediately after the excitation the hot electrons were detected as shown in Fig.1(a),which are due to the slow ionization caused by the collision between hot Rydberg atoms and cold Rydberg atoms[1].A pulsed electric field(~300 mV/cm)was applied after few microseconds and obtained a second peak,that indicates the plasma electrons.