Simultaneous occurrence of drought and heat stress will have significant negative impact on rice yield,especially under upland conditions.The projected increase in global temperatures and reduced precipitation will in...Simultaneous occurrence of drought and heat stress will have significant negative impact on rice yield,especially under upland conditions.The projected increase in global temperatures and reduced precipitation will increase the frequency of occurrence and intensity of these stresses,threatening rice production.Despite recognizing the importance of combined stress in rice,the knowledge generated in this area is very limited.Though complex,understanding combined stress tolerance of rice under water saving cultivation is more critical towards development of climate resilient rice cultivars.Here,we summarized the effects of combined stress on rice physiology with more emphasis on reproductive stage.Omics responses,phenotyping and physiology challenges and potential strategies for improving combined stress tolerance in rice are also discussed.展开更多
Plant WRKY transcription factors （TFs） constitute one of the largest families of proteinsinvolved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKYdomain at the N-terminal and a z...Plant WRKY transcription factors （TFs） constitute one of the largest families of proteinsinvolved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKYdomain at the N-terminal and a zinc finger motif at the C-terminal. To examine the relevance ofOsWRKY72 in imparting salinity stress tolerance, two indica rice genotypes, Rasi （tolerant genotype）and Tellahamsa （susceptible genotype）, were used. In Rasi seedlings at 12 h under 100 mmol/L NaClstress, OsWRKY72 expression was up-regulated, whereas in Tellahamsa, it was highly up-regulated atlethal stress. Full-length OsWRKY72 cDNA was cloned from these two rice genotypes for furtheranalysis. We identified a variant, termed as OsWRKY72b that carries an additional sequence of 111 bpwithin the WRKY domain. Expression of OsWRKY72b was higher under salinity stress in Rasi than inTellahamsa. Disorder prediction of OsWRKY72b showed that the additional sequence in the WRKYdomain is ordered thereby maintaining the tertiary structure that might interact with the major groove ofDNA. Prediction of phosphorylation sites in OsWRKY72b indicated that a few serine residues could bethe potential phosphorylation sites. In this study, we firstly reported a OsWRKY72 variant that couldhave a role in abiotic stress responses.展开更多
基金SERB,DST,Govt.of India,for National Post-Doctoral fellowship(PDF/2018/003582)Ramanujan Fellowship(SE/S2/RJN-039/2016)Early Career Research Award(ECR/2018/00l942).
文摘Simultaneous occurrence of drought and heat stress will have significant negative impact on rice yield,especially under upland conditions.The projected increase in global temperatures and reduced precipitation will increase the frequency of occurrence and intensity of these stresses,threatening rice production.Despite recognizing the importance of combined stress in rice,the knowledge generated in this area is very limited.Though complex,understanding combined stress tolerance of rice under water saving cultivation is more critical towards development of climate resilient rice cultivars.Here,we summarized the effects of combined stress on rice physiology with more emphasis on reproductive stage.Omics responses,phenotyping and physiology challenges and potential strategies for improving combined stress tolerance in rice are also discussed.
基金Niche Area of Excellence-Indian Council for Agriculture Research[Grant No.10(15)/2012]Department of Science and Technology-Fund for Improvement of Science and Technology Infrastructure Government of India for providing financial support
文摘Plant WRKY transcription factors （TFs） constitute one of the largest families of proteinsinvolved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKYdomain at the N-terminal and a zinc finger motif at the C-terminal. To examine the relevance ofOsWRKY72 in imparting salinity stress tolerance, two indica rice genotypes, Rasi （tolerant genotype）and Tellahamsa （susceptible genotype）, were used. In Rasi seedlings at 12 h under 100 mmol/L NaClstress, OsWRKY72 expression was up-regulated, whereas in Tellahamsa, it was highly up-regulated atlethal stress. Full-length OsWRKY72 cDNA was cloned from these two rice genotypes for furtheranalysis. We identified a variant, termed as OsWRKY72b that carries an additional sequence of 111 bpwithin the WRKY domain. Expression of OsWRKY72b was higher under salinity stress in Rasi than inTellahamsa. Disorder prediction of OsWRKY72b showed that the additional sequence in the WRKYdomain is ordered thereby maintaining the tertiary structure that might interact with the major groove ofDNA. Prediction of phosphorylation sites in OsWRKY72b indicated that a few serine residues could bethe potential phosphorylation sites. In this study, we firstly reported a OsWRKY72 variant that couldhave a role in abiotic stress responses.
