北部湾盆地涠西南凹陷涠洲组断裂—岩性复合圈闭发育模式与勘探实践

为了解决北部湾盆地涠西南凹陷勘探目标搜索和油田可持续开发难度大的问题,对该区二号断裂带渐新统涠洲组三段构造圈闭发育层段开展了断裂—岩性复合圈闭研究。通过对控洼断裂演化与涠三段沉积特征关系的研究,发现涠三段沉积期二号断裂带活动性较弱,凹陷长轴方向辫状河三角洲水下分流河道向洼中持续推进;涠二段、涠一段沉积期区域构造活动强烈,产生一系列雁列式断裂,辫状河三角洲前缘形成的相对孤立的水下分流河道与呈雁列式断裂形成角度交错相互切割,从而形成了NE—SW向靠断层封堵、NW—SE向靠砂体尖灭的特色河道型构造—岩性圈闭群。研究表明:(1)涠三段发育“二号断裂带洼中断隆背景、低频摆动水道、雁列张扭断裂”三因素耦合的断裂—岩性型圈闭模式,辫状河三角洲水下分流河道主流线与二号断裂带的交会区是构造—岩性圈闭有利发育区带;(2)近物源交会区主要发育复合型构造—岩性圈闭,远物源交会区主要发育孤立型构造—岩性圈闭,建立了凹陷轴向河道控砂,断裂、砂体复合控藏的成藏模式。断裂—岩性型圈闭模式和成藏模式应用效果好,搜索了一批NE—SW向靠断层封堵、NW—SE向靠砂体尖灭的有利断裂—岩性复合圈闭群,并在涠B-E构造得到很好的应用,WB-E 1井钻遇油层近100 m,后续多口井钻探成功,新增油气探明地质储量超千万立方米,证实二号断裂带涠三段断裂—岩性复合圈闭是重要的勘探领域。

南海西部原油领域勘探进展与突破方向

南海西部原油领域历经多年勘探,探明程度高,勘探难度日益增大。近年来,转变思路,大力拓展新领域。攻关岩性圈闭发育机制,建立了流三段“低位转换带控砂,湖侵互层叠置连片”的广覆式砂体沉积模式,阐明了大面积连片岩性圈闭形成机理,创建了“近源广覆式有序富集”成藏模式,发现南海西部首个大中型岩性油田;攻关深层大型储集体和优质储层发育机制,建立构造-层序-地貌耦合控砂分析技术,提出了限制型辫状河三角洲发育模式,明确了相带与溶蚀差异控储机制,涠西南凹陷B洼深层、乌石凹陷反转构造带倾末端、文昌B凹陷双古领域获得了勘探突破。同时明确了大型构造脊大型储集体耦合的深层领域、富烃凹陷周缘地层-岩性圈闭领域、源储对接潜山领域和烃源条件落实边部凹陷是南海西部原油领域下步重点勘探方向。

Thrombopoietin ameliorates doxorubicin-induced toxicities in H9c2 myocardiocytes by inhibiting oxidative stress through the SIRT1/p38 MAPK signaling pathway

Objective:To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1(SIRT1)signaling pathway.Methods:H9c2 cell viability was determined by CCK-8 and cardiomyocyte apoptosis was detected by TUNEL assay.The protein expressions of SIRT1 and p38 MAPK were measured by Western blot.RT-qPCR was also used to determine SIRT1 mRNA expression.In addition,intracellular reactive oxygen species levels and antioxidant enzyme activities were evaluated.Results:Thrombopoietin treatment reversed doxorubicin-induced decline in H9c2 cell viability.It also increased SIRT1 and decreased p-p38 MAPK protein expressions.In addition,thrombopoietin significantly attenuated doxorubicin-induced apoptosis and oxidative stress,and enhanced antioxidant enzyme activities.However,silencing SIRT1 abrogated the protective effects of thrombopoietin,as evidenced by reduced cell viability and increased oxidative stress and reactive oxygen species levels.Conclusions:Thrombopoietin alleviates doxorubicin-induced cardiomyocyte injury by reducing oxidative stress and apoptosis via the SIRT1/p38 MAPK pathway.However,its protective effects need to be further verified in animal tests.

Mechanistic study of lipid metabolism disorders in diabetic kidney disease treated with GLQMP based on network pharmacology,molecular docking and in vitro experiments

Background:In this study,we used network pharmacology and molecular docking combined with vitro experiments to explore the potential mechanism of action of Gualou Qumai pill(GLQMP)against DKD.Methods:We screened effective compounds and drug targets using Chinese medicine systemic pharmacology database and analysis platform and Chinese medicine molecular mechanism bioinformatics analysis tools;and searched for DKD targets using human online Mendelian genetics and gene cards.The potential targets of GLQMP for DKD were obtained through the intersection of drug targets and disease targets.Cytoscape software was applied to build herbal medicine-active compound-target-disease networks and analyze them;protein-protein interaction networks were analyzed using the STRING database platform;gene ontology and Kyoto Encyclopedia of Genes and Genomes were used for gene ontology and gene and genome encyclopedia to enrich potential targets using the DAVID database;and the AutoDock Vina 1.1.2 software for molecular docking of key targets with corresponding key components.In vitro experiments were validated by CCK8,oil red O staining,TC,TG,RT-qPCR,and Western blot.Results:Through network pharmacology analysis,a total of 99 potential therapeutic targets of GLQMP for DKD and the corresponding 38 active compounds were obtained,and 5 core compounds were identified.By constructing the protein-protein interaction network and performing network topology analysis,we found that PPARA and PPARG were the key targets,and then we molecularly docked these two key targets with the 38 active compounds,especially the 5 core compounds,and found that PPARA and PPARG had good binding ability with a variety of compounds.In vitro experiments showed that GLQMP was able to ameliorate HK-2 cell injury under high glucose stress,improve cell viability,reduce TC and TG levels as well as decrease the accumulation of lipid droplets,and RT-qPCR and Western blot confirmed that GLQMP was able to promote the expression levels of PPARA and PPARG.Conclusion:Overall,this study revealed the active compounds,important targets and possible mechanisms of GLQMP treatment for DKD,and conducted preliminary verification experiments on its correctness,provided novel insights into the treatment of DKD by GLQMP.

Exploring the molecular mechanism of Suoquan pill in the treatment of diabetic kidney disease based on network pharmacology,molecular docking,in vitro experiment

Background:Diabetic kidney disease(DKD)is a microvascular complication of diabetes mellitus and is the main cause of end-stage renal failure.Suoquan pills(SQP)has a variety of pharmacological activities and multiple therapeutic effects,and it is used clinically as a basic formula for the treatment of DKD.Methods:Public databases were used to identify SQP compounds and the potential targets of SQP and DKD.A drug-component-therapeutic target network was constructed.Protein-protein interaction network analysis,Gene Ontology functional analysis,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to analyse the potential molecular mechanisms of SQP based on common targets of drugs and diseases.Molecular docking simulations were conducted to confirm the binding abity of the core compounds to key targets.The efficacy and predicted molecular mechanisms of SQP were validated using cell counting kit-8 assay,flow cytometry,and western blotting with HK-2 cells as a model.Results:Network pharmacology analysis showed that 26 compounds and 207 potential targets of SQP were involved in the treatment of DKD;boldine,denudatin B,pinocembrin,kaempferoid,and quercetin were considered core compounds,and epidermal growth factor receptor(EGFR)and proto-oncogene,non-receptor tyrosine kinase(SRC)were considered key targets.Gene Ontology enrichment analysis indicated that protein phosphorylation and negative regulation of apoptotic processes are important biological processes in the treatment of DKD by SQP.Molecular docking confirmed the excellent binding abilities of boldine,denudatin B,kaempferide,and quercetin to EGFR and SRC.The results of in vitro experiments showed that treatment with an ethanolic extract of SQP significantly protected HK-2 cells from high glucose-induced cell damage.In addition,the SQP ethanol extract inhibited the phosphorylation of EGFR and SRC,suppressed the apoptosis rate,and regulated apoptosis-related proteins in HK-2 cells under high glucose stress.Conclusion:This study systematically and intuitively illustrated the possible pharmacological mechanisms of SQP against DKD through multiple components,targets,and signalling pathways,especially the inhibition of EGFR and SRC phosphorylation and apoptosis.

北部湾盆地涠西南凹陷始新统流一段湖底扇发育特征及成藏模式

根据三维地震、录井、测井、岩心观察等资料,对北部湾盆地涠西南凹陷始新统流沙港组一段湖底扇沉积特征和主控因素进行了研究,并对该区岩性圈闭发育模式及油气成藏模式进行了刻画。研究结果表明:(1)涠西南凹陷A洼在流一段低位域和湖侵域发育湖底扇沉积体,其中,低位域湖底扇发育于一号断层附近,地震上表现为连续强反射特征;湖侵域湖底扇分布于洼中,地震上具有明显的丘状反射特征。(2)受物源供给和古地貌两大因素的控制,A洼流一段发育2期湖底扇沉积。低位域短轴方向物源供给少,一号断层下降盘为洼陷的堆积中心,发育小规模直接入湖的近岸水下扇体,并形成向物源区方向上倾尖灭的岩性圈闭;湖侵域物源供给逐渐增大,一号断层活动性减弱,A洼堆积中心逐渐迁移至洼中,扇三角洲前缘松散沉积物发生二次搬运,在洼中形成湖底扇,并形成向南斜坡翘倾尖灭的岩性圈闭和被二号断层遮挡形成的构造-岩性复合圈闭。(3)垂向运移条件是研究区岩性油藏成藏的关键要素,A洼烃源层上部发育受早期断层和晚期构造差异沉降两方面因素控制的微裂缝,为油气突破泥岩盖层垂向运移至“源上”岩性圈闭内并聚集成藏提供了良好的运移通道。该区具有“超压驱动、微裂缝运移、低势区汇聚”的成藏模式。

Network pharmacology and verification experiment-based prediction of active components and potential targets of Alpiniae Oxyphyllae Fructus-Saposhnikoviae Radix(Yizhiren-Fangfeng)for treatment of diabetic kidney disease

Background:In this study,we analyzed the potential active components,related crucial targets and possible signaling pathway mechanisms of Alpiniae Oxyphyllae Fructus and Saposhnikoviae Radix(AOF-SR)herb pairs in the treatment of diabetic kidney disease(DKD)using network pharmacology and verification experiments.Methods:The active compounds and potential targets of AOF-SR were derived from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,The Encyclopedia of Traditional Chinese Medicine,and PubChem databases,and the potential therapeutic targets of DKD were derived from the OMIM,Drugbank,and DisGeNET databases.The“compounds-diseases-targets”network was constructed using Cytoscape 3.6.0.ClusterMaker functionality in Cytoscape is being used to screen important targets for AOF-SR treatment of DKD.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of important targets were performed using DAVID database.In addition,according to the predicted results of network pharmacology,HK-2 cells were used to construct DKD model for verification experiment.HK-2 cells were divided into control group,high glucose(HG)group and AOF-SR(HG+AOF-SR)group to detect survival rate and expression of key proteins in NF-κB and PI3K/Akt signaling pathways.Results:A total of 38 compounds were selected from AOF-SR,of which 23 were Alpiniae Oxyphyllae Fructus and 15 were Saposhnikoviae Radix.Through enrichment analysis of 82 important targets,88 signaling pathways were identified;some of these pathways,such as the NF-κB,PI3K-Akt,IL-17,and JAK/STAT signaling pathways,regulate the pathological process of DKD.In verification experiment,the HK-2 cells survival rate was higher in the HG+AOF-SR group than in the HG group(P<0.05).Moreover,western blotting results showed that the expression levels of NF-κB,p-PI3K,and p-Akt in HG+AOF-SR group were significantly lower than those in HG group(P<0.05).Conclusion:Overall,this study revealed the active compounds,important targets and possible mechanisms of AOF-SR treatment for DKD,and conducted preliminary verification experiments on its correctness,provided novel insights into the treatment of DKD by AOF-SR.

Network pharmacology and experimental validation to reveal the anti-hepatitis B virus pharmacological mechanism of Phyllanthus urinaria L.

Background:To explore the pharmacological mechanism of the anti-hepatitis B virus of Phyllanthus urinaria L.through network pharmacological analysis and experimental validation.Method:The active ingredient,target of action and target of action related to hepatitis B were clarified by searching the herb group identification,GeneCards and OMIM databases,and the protein interaction relationship was obtained by using the String database,and the protein interaction network map was constructed by using Cytoscape software.We also performed gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of key targets of the anti-hepatitis B action of Phyllanthus urinaria L.and predicted the core targets and pathways of Phyllanthus urinaria L.anti-hepatitis B.The main targets predicted by network pharmacology were then validated by HepG2.2.15 cell experiments.Results:By searching active ingredient targets and hepatitis B disease targets,a total of 19 active ingredients and 64 related targets of action were retrieved from Phyllanthus urinaria L.,and a total of 51 common targets were obtained by mapping the obtained hepatitis B disease targets and drug targets.protein protein interaction network analysis indicated that targets including TNF,JUN,AKT1,IL-10,IL-1B,CAT,HMOX1,NFE2L2,and CASP3 and other targets may be the core targets.gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the treatment of hepatitis B by Phyllanthus urinaria L.mainly included inflammation and oxidation-related processes,and the signaling pathways mainly included fluid shear stress and atherosclerosis,VEGF,and hepatocellular carcinoma.The results of the in vitro test showed that after the action of different concentrations of the extracts of the Phyllanthus urinaria L.in the safe concentration range on cells HepG2.2.15,HBsAg,HBeAg and hepatitis B virus DNA levels were significantly inhibited,and NFE2L2 and HMOX1 were affecting hepatitis B virus transcription and replication by regulating the oxidative stress response.Conclusion:Using an integrated network pharmacology approach,this study revealed the active components and potential targets of Phyllanthus urinaria L.for the treatment of the hepatitis B virus,providing a theoretical basis for the research and clinical application of Phyllanthus urinaria L..

Traditional Chinese medicine for the treatment of endometriosis: a comprehensive-review of treatment mechanisms

The study aims to reveal the mechanism of traditional Chinese medicine in the treatment of endometriosis and provide support for further research on traditional Chinese medicine and its active ingredients.The literature survey was conducted using PubMed,Web of Science,CNKI and other databases with“endometriosis”,“Chinese medicine”,“Chinese herbal medicine”and“natural plants”as the main keywords.Traditional Chinese medicine can exert their therapeutic effects on endometriosis by inhibiting inflammation through multiple pathways and multiple targets,regulating cell proliferation and apoptosis,angiogenesis,cell adhesion and oxidative stress.Traditional Chinese medicine can intervene in endometriosis in multiple ways and with multiple targets.In-depth study of Chinese medicine for endometriosis can provide new ideas for exploring the pathogenesis of endometriosis and developing new drugs.

北部湾盆地涠西南凹陷雁列断层变换带发育特征及其控储作用--以古近系流沙港组一段重力流沉积为例

北部湾盆地涠西南凹陷二号断裂带由一组雁列式正断层组成,断层相互叠接部位形成大型变换带,前人对该大型变换带的控储作用一直缺乏系统的研究。以三维地震和钻井资料为研究基础,对二号断裂变换带的展布特征、演化过程及控储作用开展了系统分析。二号断裂主要受新生代两期伸展活动的影响:早始新世,受北西-南东向伸展应力控制,基底断层复活,形成北东向的单一、连续大断层;渐新世中、晚期,伸展应力转为近南北向,基底断层活动减弱,上部盖层内发育分段式、雁列式断层,断层联接部位形成变换斜坡,并控制了B洼流沙港组一段的重力流沉积体发育。西北物源大型三角洲经由各分段断层间的变换斜坡进入B洼,在重力作用下发生二次搬运形成富砂湖底扇,钻井证实B洼流沙港组一段重力流沉积体呈北东向平行于二号雁列式断裂分布,沉积类型主要为砂质碎屑流。该认识为深洼带流沙港组一段岩性圈闭的勘探提供了理论依据,并取得了较好的勘探成效。

琼东南盆地深水区泥-流体底辟发育特征与天然气成藏

琼东南盆地深水区发育泥-流体底辟构造,为油气垂向运移提供通道。文章基于琼东南区域构造与沉积演化认识,利用高精度三维地震资料,对深水区底辟构造进行了系统研究,识别出了底辟的分布范围,并分析了其发育机制以及对天然气成藏的控制作用。琼东南盆地深水区底辟主要发育在乐东—陵水凹陷结合部的陵南斜坡带及凸起之上,向凹陷中心规模逐渐减小。新近纪时期盆地快速沉降和生烃作用共同导致了深部异常高压的存在,其为底辟发育提供了原始的驱动力;陵南斜坡带古近纪发育的断裂为区域内的构造薄弱带,且在新近系发生活化为异常高压释放提供突破口,进而导致了底辟的形成,即底辟形成时间为新构造运动时期。底辟构造可沟通崖城组烃源岩及黄流组、莺歌海组储集体,是深水西区油气垂向运移的主要通道,控制了天然气藏的分布。根据底辟构造与烃源、储层的配置关系,提出了陵南斜坡带和松南低凸起区是深水区下步勘探的重点区带。

乌石凹陷“背形负花构造”成因及油气富集规律差异性研究

北部湾盆地乌石凹陷发育大型"背形负花构造",具备良好的油气成藏条件,但其形成及演化机制认识不清,油气富集规律尚未明确,影响了勘探部署。本文利用高品质三维地震资料,结合构造背景研究与钻井资料分析,提出本区"背形负花构造"是伸展应力场长期作用于本区并发生顺时针旋转的结果。构造形成分为两个阶段:首先是重力滑脱作用形成了背斜构造及伴生的正断层组;然后是相对于先存断裂的斜向拉伸作用造成断裂体系的顺时针扭动,从而形成了"似花状"的结构。乌石凹陷东区构造区"花心""花瓣"油气富集差异性明显,"花心"区油气运移更加活跃,但成藏规模小,"花瓣"区成藏层系相对单一,但成藏规模大,可根据构造区位置采取相应的勘探策略。

涠西南凹陷流一段高位域早期湖底扇沉积特征及有利储层预测

涠西南凹陷流一段高位域早期湖底扇是重要的勘探层系,有利储层展布刻画不清,制约了勘探进程。基于大量岩心观察、钻井和化验分析资料及新处理三维地震资料,对其源汇体系、沉积模式及砂体展布等进行深入研究,在此基础上预测了有利储层发育位置。结果表明:涠西南凹陷流一段高位域早期在B洼发育湖底扇沉积,发育滑动滑塌、碎屑流及浊流等多种典型流态,物源主要来自东南物源扇三角洲,经断裂坡折带限流分东、西两支沉积在湖盆水体深处形成,这一新认识打破了之前混源的观点,认识到该区湖底扇相对富泥的特征,只有近物源发育的砂质碎屑流及高密度浊流沉积相对富砂,地震上主要表现为下切明显,透镜体外形清楚,朵叶体内部同相轴杂乱不连续的反射特征,综合分析预测涠西南凹陷B洼流一段高位域早期湖底扇西侧支2、5、6朵叶相对更具有勘探价值。

CeO_(2)复合碳基催化剂的氧还原性能影响因素的探究

以六水合氯化铈为前驱体、氯化钠为模板、牛奶为碳源,制备CeO_(2)修饰的三维氮、磷掺杂的碳基催化剂(CeO_(2)-NPC)。通过旋转圆盘电极(RDE)以及旋转环盘电极(RRDE),分析催化剂的制备条件对氧还原反应(ORR)过程的影响;结合X射线光电子能谱(XPS)、拉曼光谱等表征,探究合成过程对结构的影响。通过改变煅烧温度发现,温度升高的过程中N、P等杂元素会随着小分子溢出,碳载体上的缺陷程度增加,CeO_(2)的质量分数一直处于上升趋势。

Effects of Suoquan Yishen Formula on Mice with Diabetic Nephropathy (DN)

[Objectives]The purpose of this paper was to study the effects of Suoquan Yishen formula on mice with diabetic nephropathy(DN).[Methods]The mice were randomly divided into normal group,model group,irbesartan group,and high and low-dose Suoquan Yishen formula groups.The blood glucose level,BUN,Scr excretion and GSH activity and CAT activity were detected.[Results]The blood glucose levels of mice in the irbesartan group and high and low-dose Suoquan Yishen formula groups decreased.The BUN,PRO and Scr levels were higher and the GSH and CAT activity was lower in the model group compared with the normal group.After administration of Suoquan Yishen formula,the BUN,PRO and Scr levels of the DN mice decreased significantly,and the GSH and CAT activity increased significantly.[Conclusions]Suoquan Yishen formula can reduce blood glucose level,reduce PRO,BUN and Scr levels,and increase CAT and GSH activity in DN mice,thus protecting the ND mice.

Research Based on the Development Trend of World Language

Multicoloured languages play an irreplaceable role in the whole world as a useful communication tool. With the development of technology and science, varieties of languages have an ideal prospective tendency to evolution during the long and wonderful history. Will they be thriving or decaying? To begin with, aimed to gain general tendency about the quantity of languages' speakers, we employ the Grey prediction to capture associative curve which can be seen in Figure 1. From the trend of this vivid figure, we not only can come to the conclusion that the number of English and Chinese users tend to increase but also find that Spanish development will reach the period of stagnation. Secondly, for further improvement, we take birth rate, death rate, economic factors and the immigration into consideration and establish the language communication model. This model is deduced from the population prediction model and virus transmission model. After data normalization, the eventual curve indicates that current top-ten languages seem to be replaced by other languages. This transformation phenomenon also occurs among such top-ten languages. For instance, Hindustani will replace Spanish in the future when seen from Table 1. What's more, after predicting the migration pattern, we can draw the conclusion that some range of languages' dissemination has obvious change. As show in vivid Figure 14, we know English will popularize widely among neighboring countries such as Canada, Mexico, Cuba and Russia. Moreover, with regard to how to manage international offices' quantity and locations in the world, we construct the efficiency model with combination of the Bayes' probability theory and Fussy comprehensive assessment. As a result, we obtain 9 optimal plans to establish the international offices. Intelligible result is showed in Table 4 and Table 5. In short, our model is reasonable and feasible, which can accommodate to different situation.

The functional components and mechanism of Linderae Radix in treating diabetic nephropathy based on the network pharmacology

Objective:To analyze the components and mechanisms of Wuyao(Linderae Radix)in treating diabetic nephropathy(DN)based on network pharmacology.Methods:Multiple online databases were used to search and screen out the active ingredients from Linderae Radix,the related targets of active components of Linderae Radix and the genes related to DN.Search the corresponding genes name of target through UniProt database.Cytoscape 3.2.1 was used to construct the corresponding target gene network of Linderae Radix compounds.Venn diagram was used to screen the intersection genes of the active components corresponding to the target and disease-related genes,and the intersection genes were constructed into the protein interaction relationship network.Finally,DAVID database was used to do GO function enrichment analysis and KEGG signaling pathway enrichment analysis for the intersection genes,and the results of GO and KEGG were visualized.Results:1.A total of 7 potential active ingredients and 100 target proteins were screened.2.There are a total of 34 intersection genes between the potential active ingredient target in Linderae Radix in DN.3.The top 10 of the interaction correlation between intersection proteins include:AR and NCOA2,NCOA2 and NR3C1,NCOA2 and PPARG,etc.4.There were 16 entries of molecular function,9 entries of cell component,47 entries of biological process and 18 entries of signaling pathway(P<0.05).Conclusion:DN was treated by Linderae Radix from multi-component,multi-target and multi-link synergies.

北部湾盆地油气成藏规律与勘探新领域、新类型、资源潜力

北部湾盆地是南海西部海域重要的原油勘探区和产区,具备多凹陷含油、多层系成藏、多种油气成藏模式等优势成藏条件,近年来在页岩油、潜山、岩性、深层油气、后备凹陷/洼陷等新领域、新类型油气勘探中取得了突破。为了落实北部湾盆地重要生烃凹陷的区域成藏规律,指导后续常规油气精细勘探开发及新领域、新类型油气勘探,对区域石油地质条件开展了研究,总结了成藏规律。研究结果表明,多期构造运动控制了北部湾盆地的构造演化和物质充填;古近系始新统流沙港组二段油页岩和半深湖亚相泥岩为盆地最重要的烃源岩,此外,在流沙港组三段、流沙港组一段、涠洲组二段也发育多套烃源岩;发育潜山储层和碎屑岩储层,形成了多源、多储、多盖的格局;凹陷区原生近源成藏、凹中断裂带/断隆带/陡坡带垂向成藏、斜坡区过路成藏、凸起/隆起区汇流成藏等多种成藏模式控制了北部湾盆地的多凹陷成藏;油气在多凹多带有序分布,油气的差异聚集控制了内气外油、深气浅油的油气分布特征,不同区带内的油气藏类型差异显著。在区域石油地质条件与成藏规律的指导下,从资源潜力、成藏条件、勘探开发程度综合分析,页岩油、潜山、岩性圈闭、深层油气、后备凹陷/洼陷是北部湾盆地未来勘探开发的重要领域。

The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development

TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways,contributing to immune responses,tumor progression,and embryonic development.Somatic mutations within the coiled-coil(CC)domain and WD40 repeat domain of TRAF7 could cause brain tumors,while germline pathogenic mutations contribute to severe developmental abnormalities.However,the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains unclear.In this study,we employed zebrafish as an in vivo model system.TRAF7 knock down caused defects in zebrafish embryonic development.We determined the crystal structure of TRAF7 CC domain at 3.3Åresolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic development.Additionally,disease-causing mutations in TRAF7 CC region could impair the trimer formation,consequently impacting early embryonic development of zebrafish.Therefore,our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.

Molecular Diversity of Three-Component Reaction of β-Enamino Imide, Malononitrile and Cyclic α-Diketones

The base promoted three-component reaction of β-enamino imide, malononitrile and various cyclic α-diketones in acetonitrile showed interesting molecular diversity. The reactions with acenaphthylene-1,2-dione and ninhydrin afforded functionalized spiro[indene-2,4＇-pyrrolo[3,4-b]pyridines] and spiro[acenaphthylene-1,4＇-pyrrolo[3,4-b]- pyridines] in good yields. The similar reaction of phenanthrene-9,10-dione resulted in the formation of the unexpected complex phenanthro[9＇, 10＇：4,5]furo[2,3-c]pyrrolo[3,4-b]pyrroles in satisfactory yields.