Transcranial pulse current stimulation improves the locomotor function in a rat model of stroke

Previous studies have shown that transcranial pulse current stimulation(tPCS) can increase cerebral neural plasticity and improve patients' locomotor function.However, the precise mechanisms underlying this effect remain unclear.In the present study, rat models of stroke established by occlusion of the right cerebral middle artery were subjected to tPCS, 20 minutes per day for 7 successive days.tPCS significantly reduced the Bederson score, increased the foot print area of the affected limbs, and reduced the standing time of affected limbs of rats with stroke compared with that before intervention.Immunofluorescence staining and western blot assay revealed that tPCS significantly increased the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.This finding suggests that tPCS can improve the locomotor function of rats with stroke by regulating the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.These findings may provide a new method for the clinical treatment of poststroke motor dysfunction and a theoretical basis for clinical application of tPCS.The study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine of China(approval No.PZSHUTCM190315003) on February 22, 2019.

Climate warming is significantly influenced by rising summer maximum temperatures: insights from tree-ring evidence of the Western Tianshan Mountains, China

As one of the regions most affected by global cli-mate warming,the Tianshan mountains has experienced sev-eral ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Moun-tains was used to reconstruct the summer(June-August)maximum temperature(T_(max6-8))variations from 1718 to 2017.The reconstruction explained 53.1% of the variance in the observed T_(max6-8).Over the past 300 years,the T_(max6-8)reconstruction showed clear interannual and decadal vari-abilities.There was a significant warming trend(0.18°C/decade)after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The T_(max6-8) variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the sum-mer North Atlantic Oscillation.This study reveals that cli-mate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mech-anisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

Climate warming is significantly influenced by rising summer maximum temperatures:insights from tree-ring evidence of the Western Tianshan Mountains,China

As one of the regions most affected by global climate warming,the Tianshan mountains has experienced several ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Mountains was used to reconstruct the summer(June-August)maximum temperature(Tmax6-8) variations from 1718 to2017.The reconstruction explained 53.1% of the variance in the observed Tmax6-8.Over the past 300 years,the Tmax6-8reconstruction showed clear interannual and decadal variabilities.There was a significant warming trend(0.18 ℃/decade) after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The Tmax6-8variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the summer North Atlantic Oscillation.This study reveals that climate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mechanisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

生殖支原体感染与黏液脓性宫颈炎的相关性研究

目的探讨生殖支原体感染与黏液脓性宫颈炎的相关性,并分析影响黏液脓性宫颈炎患者支原体感染检出率的因素。方法选取2016年7月至2017年5月亳州市人民医院诊治的400例宫颈筛查患者的临床资料作回顾性分析。观察黏液脓性宫颈炎发病率和黏液脓性宫颈炎患者支原体感染检出阳性率,比较不同特征黏液脓性宫颈炎患者支原体感染检出阳性率的差异,分析影响黏液脓性宫颈炎患者支原体感染检出阳性率的因素。结果 400例接受宫颈筛查患者中黏液脓性宫颈炎者65例,发生率为16.25%;黏液脓性宫颈炎组患者支原体检出阳性率为81.54%,明显高于非黏液脓性宫颈炎组;有异位妊娠史、有附件压痛、宫颈涂片白细胞计数≥10/视野、有宫颈糜烂、有黏液脓性分泌物、流产次数≥2次的黏液脓性宫颈炎患者支原体感染检出阳性率较高;将单因素分析有意义的因素作为自变量,将黏液脓性宫颈炎患者支原体感染检出阳性率作为因变量,进行Logistic回归分析,结果显示,有无附件压痛、宫颈糜烂和黏液脓性分泌物是影响液脓性宫颈炎患者支原体感染检出阳性率的因素。结论黏液脓性宫颈炎患者的支原体感染阳性检出率较高,且与有无附件压痛、宫颈糜烂和黏液脓性分泌物相关。

Isolation and <i>Ex Vivo</i>Expansion of Human Hematopoietic Stem Cells Derived from Umbilical Cord Blood

Umbilical cord blood (UCB) is a current major source of hematopoietic stem cells (HSCs) for cell transplantation therapy. Cell transplantation with HSCs derived from UCB is advantageous over transplantation with HSCs from adult tissues. However, the low number of HSC derived from a single unit of UCB limits its application. Thus, ex vivo expansion is a good option to create more UCB HSCs for clinical application. The strategies for HSC expansion in vitro focus on mimicking the composition and structure of HSC natural niche by enhancing self-renewal and inhibiting lineage differentiation of HSCs. In the past decade, the mechanisms of the interaction between HSC and the natural niche have been deeply investigated. This great progress in basic research has led to advancements in UCB HSC ex vivo expansion. In addition, the biological characteristics of the originally isolated UCB HSCs correlate with outcome of subsequent ex vivo expansion. In this paper, we summarize the late progress achieved in isolation and ex vivo expansion of UCB HSCs. Importantly, we attempt to provide an impact and practicable procedure to expand UCB HSC in vitro from isolation of original HSCs to identification of expanded HSCs.

基于KPCA和BiLSTM的分解炉出口温度预测

水泥生产过程中,分解炉出口温度是非常重要的工艺参数,为了应对出口温度变量的多样性,文章提出一种核主成分分析(kernel principal component analysis,KPCA)与双向长短期记忆(bidirectional long short-term memory,BiLSTM)神经网络相结合的温度预测组合模型用来预测分解炉的出口温度。通过KPCA筛选出影响因素的主成分从而达到数据降维目的,将降维后的主成分作为BiLSTM神经网络的输入,分解炉出口温度作为BiLSTM神经网络的输出。经BiLSTM神经网络训练,得到分解炉出口温度预测模型。通过对比验证表明,使用KPCA-BiLSTM相结合的温度预测模型具有较好的预测精度。

肺癌筛查研究进展

肺癌是世界上最常见的恶性肿瘤,其5年生存率仅为19.7%,严重威胁人类健康。肺癌筛查是降低肺癌死亡率的有效措施,已有的研究证明用低剂量螺旋计算机断层扫描(low-dose computed tomography,LDCT)进行筛查可降低20%的肺癌死亡,目前国际和国内均建议进行肺癌筛查。研究肺癌筛查的发展现状有助于我们明确肺癌的高危人群,探索合理的筛查方案,提高筛查的成本效益,减轻经济负担。因此本文就肺癌筛查现状、肺癌筛查的成本效益以及存在的问题综述如下。

聚氯代对二甲苯/石墨烯高阻水复合膜的制备与性能

聚氯代对二甲苯膜(PC膜)在防潮、封装等领域具有重要应用,但因其本体结构限制而不能满足电子、军工等对高阻水、柔性膜的要求。文中利用石墨烯膜强度高、气体阻隔性好、透光性优良和紫外固化胶黏剂快速固化的特点将PC膜与石墨烯膜进行复合,制备了聚氯代对二甲苯/石墨烯多层复合膜。红外分析表明紫外固化胶黏剂的反应程度达到99%以上,力学和水汽透过率测试表明复合膜的阻水和力学性能显著提高,水汽透过率从1.1968g/(m^(2)·d)降至4.9×10^(-3)g/(m^(2)·d),下降了2个数量级,拉伸强度和断裂伸长率分别提高了60.0%和792.4%。

Development of N-doped carbons from zeolite-templating route as potential electrode materials for symmetric supercapacitors

N-doped carbons were fabricated from zeolite-templated carbon via modification with melamine and mild KOH activation. The N-doping treatment and KOH activation slightly lowered the surface areas of pristine zeolite-templated carbon; nonetheless, N-doped carbons with a lower surface area exhibited much higher capacitance and cycling stability as fabricated into symmetric supercapacitor. Significantly, N-doped carbon obtained at 700°C showed a capacitance of 45.7 F/g at 0.1 A/g and 42.0 F/g at 10 A/g for the fabricated supercapacitor with 6 M KOH electrolyte, with 92% retention of initial capacitance as current density increased up to 100-fold. This performance was attributed to the dual contribution of electric double-layer capacitance and pseudo-capacitance. The assembled supercapacitor also exhibited excellent cycling stability, with 91% capacitance retention at 10 A/g after 10000 cycles.

The study of selective primary culture and determination of a breast cancer cell line in vitro

Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank.

Impact of 1,25-(OH)_2D_3 on Left Ventricular Hypertrophy in Type 2 Diabetic Rats

Objective To investigate the impact of 1, 25-(OH)2D3 on left ventricular hypertrophy(LVH) in type 2 diabetic rats. Methods Type 2 diabetic mellitus(DM) model rats were established by intraperitoneally injecting with 30 mg/kg streptozotocin. After 8 weeks, 19 male rats were identified as diabetic with left ventricular hypertrophy(LVH) by ultrasound examination, and randomly assigned into three groups: untreated(DM-LVH, n=7), treated with insulin(DM-LVH+INS, n=6), and treated with 1, 25-(OH)2D3(DM-LVH+VD, n=6). Healthy male rats were used as the controls group(n=6). The fasting blood glucose and the insulin level were determined weekly. The left ventricular mass index, myocardial collagen content, collagen volume fraction, and 1, 25-(OH)2D3-receptor level were determined by 4 weeks later. Results In the DM-LVH model group, the insulin level was significantly decreased compared with the non-diabetic control group(P<0.05), whereas the blood glucose, left ventricular mass index, myocardial collagen content, collagen volume fraction, and 1, 25-(OH)2D3-receptor expression were significantly increased(all P<0.05). In the DM-LVH+INS and DM-LVH+VD groups, the insulin levels were significantly increased compared with the DM-LVH model group(P<0.05), whereas the other parameters were significantly decreased(all P<0.05). Conclusion 1, 25-(OH)2D3 could reverse LVH in diabetic rats and that the mechanism may involve stimulating insulin secretion and reducing blood glucose via direct up-regulation of 1, 25-(OH)2D3-receptor expression.

Cs-content-dependent organic cation exchange in FA1-xCsxPbI3 perovskite

FA-Cs mixed-cation perovskite has been reported as a promising candidate for obtaining highly efficient and stable photovoltaic devices.Phenylethylamine iodide(PEAI)post-treatment is a widely used and effective method for surface passivation of FA-Cs perovskite layer in devices.However,it is still controversial whether the PEAI post-treatment would form two-dimensional(2D)perovskite PEA_(2)PbI_(4) capping layer or just result in PEA+terminated surface.Here in this work,the function of PEAI post-treatment on FA-Cs mixed-cation perovskite FA_(1-x)Cs_(x)PbI_(3)(x=0.1–0.9)with varied Cs contents is elucidated.With increased Cs content,the FA-Cs perovskite shows higher resistance to the cation exchange between FA+and PEA+.This Cs-content-dependent cation exchange results in the different PEAI reaction preferences with FA-Cs mixed-cation perovskites.Furthermore,higher Cs content with stronger resistance to cation exchange reaction leads to a wider processing window for post-treatment and defect passivation,which is beneficial for the fabrication of large-scale photovoltaic devices.

Ultralow-strain Ti substituted Mn-vacancy layered oxides with enhanced stability for sodium-ion batteries

Anionic redox reaction(ARR) in layered manganese-based oxide cathodes has been considered as an effective strategy to improve the energy density of sodium-ion batteries.Mn-vacancy layered oxides deliver a high ARR-related capacity with small voltage hysteresis,however,they are limited by rapid capacity degradation and poor rate capability,which arise from inferior structure changes due to repeated redox of lattice oxygen.Herein,redox-inactive Ti^(4+)is introduced to substitute partial Mn^(4+)to form Na_(2) Ti_(0.5)Mn_(2.5)O_7(Na_(4/7)[□_(1/7)Ti_(1/7)Mn_(5/7)]O_(2),□ for Mn vacancies),which can effectively restrain unfavorable interlayer gliding of Na2 Mn307 at high charge voltages,as reflected by an ultralow-strain volume variation of 0.11%.There is no irreversible O_(2) evolution observed in Na_(2) Ti_(0.5)Mn_(2.5)O_7 upon charging,which stabilizes the lattice oxygen and ensures the overall structural stability.It exhibits increased capacity retention of 79.1% after 60 cycles in Na_(2) Ti_(0.5)Mn_(2.5)O_7(17.1% in Na_(2) Mn_(3) O_7) and good rate capability(92.1 mAh g^(-1) at 0.5 A g^(-1)).This investigation provides new insights into designing high-performance cathode materials with reversible ARR and structural stability for SIBs.

Study on the antitumor effects of autologous and allogeneic CIK cells in patients with breast cancer

Objective This study aimed to compare the anti-tumor effects of cytokine-induced killer (CIK) cellsinduced by autologous cytokines in patients with breast cancer and those of allogeneic CIK cells fromhealthy adults.Methods We used conventional methods to induce CIK cells originating from two peripheral bloodmononuclear cell types (from patients with breast cancer and healthy adults). Killing activity was detectedusing an LDH assay, immunophenotypic changes were analyzed by flow cytometry, and the IFN-γ level ofculture supernatants was detected by ELISA.Results The results showed that the proliferative capacity of the allogeneic CIK cells was significantlyhigher than that of the autologous CIK cells. Compared with autologous CIK cells, the allogeneic CIK cellshad significantly enhanced anti-tumor activity against SKBR-3 cells (P < 0.01) and IFN-γ secretion (P <0.05);moreover, they increased the ratio of CD3+ CD56+ cells and CD3+ CD8+ cells (P < 0.05).Conclusion Healthy adult-derived induced CIK cells exhibited a stronger anti-tumor effect than inducedCIK cells derived from patients with breast cancer. The results of this study could provide experimentalevidence for the clinical application of CIK cells.

Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer

Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat shock protein 70-peptide complexes(HSP70-PCs)on HER-3-overexpressing breast cancer.Methods In this study,we first studied the expression of HER-3 in breast cancer tissues and its relationship with patient characteristics.We then purified HSP70-PCs from primary breast cancer cells with different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic cells(DCs)and CD8+T cells induced by these complexes.Third,recombinant human HSP70-HER-3 protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3-overexpressing breast cancer cells,and the resulting immunological response was examined.Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these treatments induce autogenous CD8+T cell killing of HER-3-positive breast cancer cells.Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients with HER-3-overexpressing breast cancer.

Analysis of Clinical Features in Novel Coronavirus Pneumonia Convalescents

Background:To analyze the clinical features of convalescent individuals with novel coronavirus-infected pneumonia(novel coronavirus pneumonia)at rehabilitation station in Wuhan.Methods:712 patients in the convalescence period of novel coronavirus pneumonia isolated and observed at the rehabilitation station were investigated by collecting their basic data,clinical syndrome features,and tongue manifestations.The clinical syndrome features were analyzed based on guidelines of Diagnosis and Treatment Plan for Novel Coronavirus-infected Pneumonia(National Health Commission of the People’s Republic of China,7th Trial Edition).Results:Cough(24.86%),chest tightness and shortness of breath(23.17%),and fatigue(16.57%)were the main symptoms of patients in the convalescence period of novel coronavirus pneumonia.Their tongue appearance was mostly characterized by redness(88.65%),thick coating(67.25%),greasy coating(49.78%)and white coating(76.86%).Conclusion:Compared with the ordinary/mild patients,cough and anorexia symptoms were more common in the severe/critical patients.In convalescent patients,red and fat tongue with thick greasy fur were common.

数字化转型背景下高校信息化建设特征与路径优化

高校信息化建设是推动教育现代化的关键途径,高校信息化规划的执行效果直接影响并体现我国的高等教育信息化水平。因此,探讨我国高校信息化建设的特征与路径优化,具有重要意义。采用量化文本分析和内容分析方法,通过质性分析软件对21所不同类型高校的信息化建设文本进行系统分析,构建包含价值层、支撑层、应用层、主体层的分析框架,以揭示高校信息化建设的特征。通过深入研究发现,高校信息化发展目标泛化,信息化评估模式亟待创新;系统建设不平衡,技术创新及应用滞后;管理与服务割裂,信息技术内驱力不足;信息化参与能力和机会不足。同时,不同类型高校既有遵循国家政策方针、重视网络基础设施建设、教育资源共享等共同点,也存在战略规划侧重、技术应用层次、组织管理体制和人才队伍建设等方面的差异。据此建议,高校应加强顶层规划,优化教育基础设施,创新技术应用,并明晰治理权责,构建信息化协同发展共同体。通过这些措施,提升高校信息化建设的整体水平,推进教育数字化转型,提高教育质量和效率。

Interfacial Chemistry Enables Highly Reversible Na Extraction/Intercalation in Layered-Oxide Cathode Materials

Comprehensive Summary Layered transition-metal oxides are promising cathode candidates for sodium-ion batteries.However,the inferior interphase formation and particulate fracture during sodiation/desodiation result in structure degradation and poor stability.Herein,the interface chemistry of P2-Na_(0.640)Ni_(0.343)Mn_(0.657)O_(2)in an electrolyte of 1.0 mol/L NaPF6 in diglyme is unveiled to enable highly reversible Na extraction and intercalation.The uniform and robust cathode-electrolyte interphase layer is in situ formed with decomposition of diglyme molecules and anions in initial cycles.The NaF-and CO-rich CEI film exhibits high mechanical strength and ionic conductivity,which suppresses the reconstruction of its electrode interphase from P2 phase to spinel-like structure and reinforces its structure integrity without cracks.This favours facile Na+transport and stable bulk redox reactions.It is demonstrated to show long cycling stability with capacity retention of 94.4%for 180 cycles and superior rate capability.This investigation highlights the cathode interphase chemistry in sodium-ion batteries.

Secondary battery inspired ɑ-nickel hydroxide as an efficient Ni-based heterogeneous catalyst for sulfate radical activation

Highly reactive radical species play an important role in syn-thetic chemistry, industrial processes, and environmental remedi-ation applications [1,2]. The radicals can be formed by eitherthermal decomposition of highly active oxidants or by catalyticactivation of stable oxidants under mild conditions [2]. Recently,the sulfate radical （.504） with its advantages of strong oxidizingpower, selective reactivity, and stability over a broad range of pHhas emerged as an excellent and versatile oxidant for a variety ofapplication [5].

MA Cation-Induced Diffusional Growth of Low-Bandgap FA-Cs Perovskites Driven by Natural Gradient Annealing

Low-bandgap formamidinium-cesium(FA-Cs)perovskites of FA_(1-x)CsxPbI_(3)(x<0:1)are promising candidates for efficient and robust perovskite solar cells,but their black-phase crystallization is very sensitive to annealing temperature.Unfortunately,the low heat conductivity of the glass substrate builds up a temperature gradient within from bottom to top and makes the initial annealing temperature of the perovskite film lower than the black-phase crystallization point(~150℃).Herein,we take advantage of such temperature gradient for the diffusional growth of high-quality FA-Cs perovskites by introducing a thermally unstable MA^(+)cation,which would firstly formα-phase FA-MA-Cs mixed perovskites with low formation energy at the hot bottom of the perovskite films in the early annealing stage.The natural gradient annealing temperature and the thermally unstable MA^(+)cation then lead to the bottom-to-top diffusional growth of highly orientatedα-phase FA-Cs perovskite,which exhibits 10-fold of enhanced crystallinity and reduced trap density(~3:85×10^(15) cm^(−3)).Eventually,such FA-Cs perovskite films were fabricated into stable solar cell devices with champion efficiency up to 23.11%,among the highest efficiency of MA-free perovskite solar cells.