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超声引导下空芯针和针吸活检对乳腺癌内乳淋巴结诊断价值的比较 被引量:10
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作者 石可心 纪晓惠 +3 位作者 魏梦莹 张喆 刘志坤 高冬霞 《中国肿瘤临床》 CAS CSCD 北大核心 2021年第19期991-995,共5页
目的:评估超声引导下空芯针活检(core needle biopsy,CNB)和针吸活检(fine needle aspiration,FNA)对乳腺癌内乳淋巴结(internal mammary node,IMN)转移的诊断价值。方法:回顾性分析2012年5月至2020年10月河北医科大学第四医院496例行... 目的:评估超声引导下空芯针活检(core needle biopsy,CNB)和针吸活检(fine needle aspiration,FNA)对乳腺癌内乳淋巴结(internal mammary node,IMN)转移的诊断价值。方法:回顾性分析2012年5月至2020年10月河北医科大学第四医院496例行超声引导下IMN穿刺的乳腺癌患者临床资料,其中CNB组374例、FNA组122例,采用一致性Kappa检验分析CNB、FNA的诊断效能,计算诊断的敏感度、特异度等指标。亚组分析不同大小及类型IMN的CNB、FNA诊断效能及影响标本满意度的因素。结果:CNB组和FNA组穿刺标本不满意度分别为5.6%(21/374)和3.3%(4/122),差异无统计学意义(P>0.05)。剔除不满意标本后比较CNB、FNA的诊断效能,其Kappa值分别为0.817、0.907。亚组分析显示IMN厚径<0.5 cm组和0.5~0.9 cm组中,CNB、FNA的Kappa值分别为0.877、1.000和0.772、0.783(均P<0.01);IMN厚径≥1.0 cm组中,CNB组的假阴性率为3.4%(1/29),FNA组无假阴性。结论:对于IMN定性诊断,FNA诊断效能更高,由于IMN一般较小且位置特殊,FNA也是更安全的穿刺方法。 展开更多
关键词 超声引导 空芯针活检 针吸活检 内乳淋巴结 乳腺癌
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Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis
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作者 wei Zhang Zhuo Wan +10 位作者 Di Qu Wenqi Sun Liang Zhang Yuan Liang Lei Pan Hua Jiang Zichen Ye mengying wei Lijun Yuan Guodong Yang Faguang Jin 《Bioactive Materials》 SCIE CSCD 2024年第2期488-501,共14页
Pulmonary fibrosis(PF)is a devastating lung disease with limited treatment options.During this pathological process,the profibrogenic macrophage subpopulation plays a crucial role,making the characterization of this s... Pulmonary fibrosis(PF)is a devastating lung disease with limited treatment options.During this pathological process,the profibrogenic macrophage subpopulation plays a crucial role,making the characterization of this subpopulation fundamentally important.The present study revealed a positive correlation between pulmonary macrophages with higher mitochondrial mass(Mø^(mitohigh))and fibrosis.Among the Mø^(mitohigh)subpopulation of CD206^(+)M2,characterized by higher expression of dynamin 1-like(Drp1),as determined by flow cytometry and RNA-seq analysis,a therapeutic intervention was developed using an exosome-based formula composed of pathfinder and therapeutics.A pathfinder exosome called“exosome^(MMP19)(Exo^(MMP19))”,was constructed to display matrix metalloproteinase-19(^(MMP19))on the surface to locally break down the excessive extracellular matrix(ECM)in the fibrotic lung.A therapeutic exosome called“exosome therapeutics(Exo^(Tx))”,was engineered to display D-mannose on the surface while encapsulating siDrp1 inside.Prior delivery of Exo^(MMP19)degraded excessive ECM and thus paved the way for Exo^(Tx)to be delivered into Mø^(mitohigh),where Exo^(Tx)inhibited mitochondrial fission and alleviated PF.This study has not only identified Mø^(mitohigh)as profibrotic macrophages but it has also provided a potent strategy to reverse PF via a combination of formulated exosomes. 展开更多
关键词 Pulmonary fibrosis MACROPHAGES Mitochondrial fission Drp1 EXOSOMES
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Adoptive transfer of metabolically reprogrammed macrophages for atherosclerosis treatment in diabetic ApoE^(-/-) mice 被引量:2
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作者 Tingting Wang Yan Dong +9 位作者 Li Yao Fan Lu Chenxi Wen Zhuo Wan Li Fan Zhelong Li Te Bu mengying wei Xuekang Yang Yi Zhang 《Bioactive Materials》 SCIE 2022年第10期82-94,共13页
Atherosclerosis is characterized by inflammation in the arterial wall,which is known to be exacerbated by diabetes.Therapeutic repression of inflammation is a promising strategy for treating atherosclerosis.In this st... Atherosclerosis is characterized by inflammation in the arterial wall,which is known to be exacerbated by diabetes.Therapeutic repression of inflammation is a promising strategy for treating atherosclerosis.In this study,we showed that diabetes aggravated atherosclerosis in apolipoproteinE knockout(ApoE^(-/-))mice,in which increased expression of long-chain acyl-CoA synthetase 1(Acsl1)in macrophages played an important role.Knockdown of Acsl1 in macrophages(Mφ^(shAcsl1))reprogrammed macrophages to an anti-inflammatory phenotype,especially under hyperglycemic conditions.Injection of Mφ^(shAcsl1) reprogrammed macrophages into streptozotocin(STZ)-induced diabetic ApoE^(-/-) mice(ApoE^(-/-)+STZ)alleviated inflammation locally in the plaque,liver and spleen.Consistent with the reduction in inflammation,plaques became smaller and more stable after the adoptive transfer of reprogrammed macrophages.Taken together,our findings indicate that increased Acsl1 expression in macrophages play a key role in aggravated atherosclerosis of diabetic mice,possibly by promoting inflammation.Adoptive transfer of Acsl1 silenced macrophages may serve as a potential therapeutic strategy for atherosclerosis. 展开更多
关键词 ATHEROSCLEROSIS DIABETES MACROPHAGE Acsl1 Adoptive transfer
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HIF1α epigenetically repressed macrophages via CRISPR/Cas9-EZH2 system for enhanced cancer immunotherapy 被引量:2
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作者 Yan Dong Siyan Zhang +8 位作者 Xiaotong Gao Dandan Yin Tingting Wang Zhelong Li Zhuo Wan mengying wei Ying Luo Guodong Yang Li Liu 《Bioactive Materials》 SCIE 2021年第9期2870-2880,共11页
Immune suppressive microenvironment in tumor emerges as the main obstacle for cancer immunotherapy.In this study,we identified that HIF1α was activated in the tumor associated macrophages and acted as an important fa... Immune suppressive microenvironment in tumor emerges as the main obstacle for cancer immunotherapy.In this study,we identified that HIF1α was activated in the tumor associated macrophages and acted as an important factor for the immune suppressive microenvironment.Epigenetically silencing of Hif1αvia histone H3 methylation in the promoter region was achieved by CRISPR/dCas9-EZH2 system,in which histone H3 methylase EZH2 was recruited to the promoter region specifically.The Hif1αsilenced macrophage,namely HERM(Hif1αEpigenetically Repressed Macrophage)manifested as inheritable tumor suppressing phenotype.In the subcutaneous B16-F10 melanoma syngeneic model,intratumoral injection of HERMs reprogrammed the immune suppressive microenvironment to the active one,reducing tumor burden and prolonging overall survival.Additionally,HERMs therapy remarkably inhibited tumor angiogenesis.Together,our study has not only identified a promising cellular and molecular target for reverting immune suppressive microenvironment,but also provided a potent strategy for reprogramming tumor microenvironment via epigenetically reprogrammed macrophages. 展开更多
关键词 Cancer immunotherapy Immune suppressive microenvironment MACROPHAGE HIF1Α CRISPR/dCas9 Epigenetically reprogrammed macrophage
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Kinetically regulated one-pot synthesis of cationic gold nanoparticles and their size-dependent antibacterial mechanism 被引量:1
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作者 Chenqiang Shen Yumeng Xue +4 位作者 Yixiao Li mengying wei Mengyao Wen Lianbing Zhang Li Shang 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2023年第31期145-156,共12页
Cationic gold nanoparticles(cAuNPs)have been regarded as promising candidates for antibacterial applications due to their high surface charge density,favorable biocompatibility,and controllable surface chemistry.Never... Cationic gold nanoparticles(cAuNPs)have been regarded as promising candidates for antibacterial applications due to their high surface charge density,favorable biocompatibility,and controllable surface chemistry.Nevertheless,the complicated fabrication process and unclear antibacterial mechanism have greatly hindered the further biomedical application of cAuNPs.Herein,we have developed a simple and controllable strategy for synthesizing cAuNPs with tailored size and antibacterial behavior by kinetically modulating the reaction process.Specifically,a functional ligand,(11-mercaptoundecyl)-N,N,Ntrimethylammonium bromide(MUTAB),was chosen to chemically manipulate the positive surface charge of cAuNPs via a one-step strategy.The size of cAuNPs could be flexibly adjusted from 1.1 to 14.8 nm by simply elevating the stirring speed of the reaction from 0 to 1500 rpm.Further studies revealed that the antibacterial effect of cAuNPs was strongly correlated with the particle size.MUTAB-protected ultrasmall gold nanoclusters(MUTAB-AuNCs)were able to eradicate E.coli at a concentration as low as 1.25μg mL^(-1),while the minimum inhibitory concentration of MUTAB-AuNPs with a large size for E.coli was 5μg mL^(-1).Mechanistic investigation revealed that MUTAB-AuNPs were able to damage the bacterial membrane and stimulate the production of reactive oxygen species more effectively than MUTAB-AuNCs.Conversely,MUTAB-AuNCs were more active in inducing membrane depolarization in contrast to MUTAB-AuNPs,suggesting the unique size-dependent antibacterial manner of cAuNPs.This study presents a new strategy for the controlled preparation of cAuNPs with distinct sizes and antibacterial behavior,laying a valuable foundation for developing efficient cationic NP-based bactericidal agents. 展开更多
关键词 Gold nanoparticles Size effect Antibacterial mechanism Reactive oxygen species Positive charge
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