Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

Hemolysis of PM_(10) on RBCs in vitro:An indoor air study in a coal-burning lung cancer epidemic area

Epidemiological studies have suggested that inhalation exposure to indoor ambient air from coal-burning environments is causally associated with respiratory health risks.In order to explore the toxicological mechanisms behind the adverse health effects,the hemolytic activity of PM_(10)(particulate matter with an aerodynamic diameter of 10 um or less)samples collected from homes burning coal in the recognized China"cancer village"Xuanwei were evaluated and matched against their trace elemental contents.The results demonstrated that the hemolytic activity of indoor PM_(10) in coal-burning environments ranged from 4.28%to 5.24%,with a clear positive dose-response relationship.Although low dose samples exhibited a reduced hemolytic activity,PM_(10) could have a toxic effect upon people in a coal-burning indoor environment for extended time periods.The concentrations of analyzed trace elements in PM_(10) samples ranged from 6966 to 12,958 ppm.Among the analyzed elements,Zn,Ti,Ni,Cu,Pb,Ba,Mn,Cr and V were found at higher concentrations and accounted for over 95%of the total elements.The concentrations of total analyzed elements in the PM_(10) samples revealed a significant positive correlation with PM_(10) hemolytic activity.Of the analyzed elements,Zn,Pb and Cs positively correlated with hemolysis,while Li,U and V negatively correlated with the hemolysis of human red blood cells(RBCs).Therefore,the heavy metal elements could be one of the main factors responsible for the hemolytic capacity of indoor PM_(10) in coal-burning environments.

The role of airborne particles and environmental considerations in the transmission of SARS-CoV-2

Corona Virus Disease 2019(COVID-19)caused by the novel coronavirus,results in an acute respiratory condition coronavirus 2(SARS-CoV-2)and is highly infectious.The recent spread of this virus has caused a global pandemic.Currently,the transmission routes of SARS-CoV-2 are being established,especially the role of environmental transmission.Here we review the environmental transmission routes and persistence of SARS-CoV-2.Recent studies have established that the transmission of this virus may occur,amongst others,in the air,water,soil,cold-chain,biota,and surface contact.It has also been found that the survival potential of the SARS-CoV-2 virus is dependent on different environmental conditions and pollution.Potentially important pathways include aerosol and fecal matter.Particulate matter may also be a carrier for SARS-CoV-2.Since microscopic particles can be easily absorbed by humans,more attention must be focused on the dissemination of these particles.These considerations are required to evolve a theoretical platform for epidemic control and to minimize the global threat from future epidemics.

Identification of a stable major QTL for fresh-seed germination on chromosome Arahy.04 in cultivated peanut(Arachis hypogaea L.)

Fresh-seed germination(FSG)impairs peanut production,especially in areas where the peanut harvest season coincides with rainy weather.Developing FSG-resistant cultivars by molecular breeding is expected to mitigate yield loss and quality impairment caused by FSG.However,the genetic control of FSG awaits elucidation.In this study,FSG at 1,3,5,7,and 9 days post-imbibition in three environments were tested,and quantitative-trait loci(QTL)associated with FSG were mapped in a peanut recombinant inbred line population by leveraging existing high-density peanut genetic maps.Of 24 QTL identified in 13 linkage groups,qFSGA04 was a stable major QTL on linkage group 04(LG04).It was consistently detected in five germination stages and three environments.By designing and validating DNA markers in the confidence interval of qFSGA04,we identified one single-nucleotide polymorphism and one In Del closely associated with FSG that could be used as linked markers for FSG resistance in peanut breeding.

A novel modified paclitaxel-loaded discoidal recombinant high-density lipoproteins:Preparation,characterizations and in vivo evaluation

This study is one of the first to focus on the unexpected drug leakage from discoidal recombinant high-density lipoproteins(d-rHDLs)as a consequence of remodeling process,mainly associated with lecithin-cholesterol acyltransferase(LCAT)during their metabolic process.Here,a newly monocholesterylsuccinate(CHS)modified paclitaxel-loaded drHDLs(cP-d-rHDLs)were constructed successfully through structural modification,thus aiming to improve the performance of d-rHDLs.And next their in vitro physiochemical properties and pharmacokinetics in SpragueeDawley rats were elaborately investigated.Collectively our studies demonstrated that cP-d-rHDLs,whose remodeling behaviors were restrained effectively after structural modification,exhibited more excellent and promising properties as novel delivery vehicles for anti-cancer agents.

Resource Scheduling Strategy for Performance Optimization Based on Heterogeneous CPU-GPU Platform

In recent years,with the development of processor architecture,heterogeneous processors including Center processing unit(CPU)and Graphics processing unit(GPU)have become the mainstream.However,due to the differences of heterogeneous core,the heterogeneous system is now facing many problems that need to be solved.In order to solve these problems,this paper try to focus on the utilization and efficiency of heterogeneous core and design some reasonable resource scheduling strategies.To improve the performance of the system,this paper proposes a combination strategy for a single task and a multi-task scheduling strategy for multiple tasks.The combination strategy consists of two sub-strategies,the first strategy improves the execution efficiency of tasks on the GPU by changing the thread organization structure.The second focuses on the working state of the efficient core and develops more reasonable workload balancing schemes to improve resource utilization of heterogeneous systems.The multi-task scheduling strategy obtains the execution efficiency of heterogeneous cores and global task information through the processing of task samples.Based on this information,an improved ant colony algorithm is used to quickly obtain a reasonable task allocation scheme,which fully utilizes the characteristics of heterogeneous cores.The experimental results show that the combination strategy reduces task execution time by 29.13%on average.In the case of processing multiple tasks,the multi-task scheduling strategy reduces the execution time by up to 23.38%based on the combined strategy.Both strategies can make better use of the resources of heterogeneous systems and significantly reduce the execution time of tasks on heterogeneous systems.

Factors controlling tight oil and gas reservoir development in the Jurassic siliciclastic-carbonate rocks in Sichuan Basin, China

The prediction of“sweet spot”of multi-lithology composite tight oil and gas reservoirs within mixed siliciclastic-carbonate sequences is a hot topic in oil and gas exploration.There are mixed lacustrine carbonate-siliciclastic rocks in the Da'anzhai Member of Ziliujing Formation in the Eastern Slope of western Sichuan Depression.The reservoir lithology is complex,and the main factors controlling the development of high-quality reservoirs are yet to be known.Based on a large number of drilling,core and thin section observation,well log and seismic data,this paper systematically studies the characteristics and factors controlling the development of the mixed siliciclastic-carbonate reservoir.The results show that the reservoir is composed primarily of coquina,sandstone,breccia and shale in lithology,and its development is mainly controlled by sedimentary microfacies,diagenesis,tectonism(fracturing)and paleogeomorphology during the sedimentary period.The effective fractures in reservoirs of the low-energy shell shoal facies are well developed with relatively good physical properties.With the change of sedimentary microfacies from low-energy shoal to high-energy shoal to arenaceous shoal,the hy-drocarbon production capacity worsens step by step.Diagenesis,such as dissolution and fracturing,has a certain effect on reservoir physical properties.The palaeogeomorphic highs and slopes had well-developed fractures during the sedimentary period which are conducive to constructive dissolution,and thus they are favorable zones for reservoir development.The development of structural fractures further amplifies the influence of dissolution;thus the fracture zones are also favorable for the devel-opment of high-quality reservoirs.The favorable sedimentary facies zone and fracture development degree serve as the core factors for the formation of high-quality mixed reservoirs.The muddy limestone with certain dissolved pores and structural fractures deposited under the low-energy shell shoal setting is of relatively high-quality reservoir in the Da'anzhai Member.

Evaluating Optimal Treatment for Melanoma:A Network Meta-Analysis

Background It is necessary to determine the optimal treatment for melanoma as the incidence of melanoma is increasing every year.In this study,we conducted a metaanalysis to compare the effectiveness of available treatments for melanoma.Methods The index keywords“melanoma”and“treatment”were used to search the PubMed,Cochrane Library,Web of Science,and Embase databases,and the articles were limited to randomized controlled trials.The search was filtered for articles published until February 2020,and articles were independently extracted by two reviewers.Network metaanalysis(Stata®14.0 software network package,StataCorp LLC,TX,USA)was used to compare the effectiveness of the mentioned treatments of melanoma.Results A total of 616 articles were screened of which 6 were selected for meta-analysis,involving 5 treatment measures and 2047 patients(those who were not followed up on were excluded).The treatment strategies reported for melanoma included tumor necrosis factor(L19-TNFα)and interleukin-2(L19IL2)in combination with dacarbazine(L19IL2+DTIC),DTIC,programmed cell death-1 plus cytotoxic T cell antigen-4(PD-1+CTLA-4),PD-1,and CTLA-4.Conclusion Among L19IL2+DTIC,DTIC,PD-1+CTLA-4,PD-1,and CTLA-4,CTLA-4 is found to be the best treatment method for melanoma.Our study findings provide a reference for the clinical treatment of melanoma and can clarify the direction for setting up controlled clinical trials.

氨基酸基防污可注射自融合水凝胶的制备及其在术后防粘连中的应用

设计和制备具有优异防污能力的可注射防粘连材料对预防手术后组织和器官的粘连具有重要的意义.在此,我们将含有羟基的氨基酸衍生物引入水凝胶的制备中,通过N-丙烯酰丝氨酸(ASer)的自由基聚合制备了一种新型的氢键交联水凝胶(PASer).结果发现一个甲基取代基的存在会对分子间氢键相互作用产生明显的影响:与聚(N-丙烯酰苏氨酸)(PAThr)水凝胶相比,PASer水凝胶具有较低的成胶浓度和增强的力学性能.值得一提的是,亲水性羟基使PASer水凝胶能够有效抵抗蛋白质吸附和细胞粘附.PASer水凝胶具有优异的力学性能、可注射性、自融合性、良好的生物相容性和适宜的体内停留时间,符合临床对术后抗粘连材料的要求.体内研究实验结果表明,PASer水凝胶在建立的小鼠腹壁缺损模型中可以有效预防术后腹膜粘连发生.

Optimal Operation with Dynamic Partitioning Strategy for Centralized Shared Energy Storage Station with Integration of Large-scale Renewable Energy

As renewable energy continues to be integrated into the grid,energy storage has become a vital technique supporting power system development.To effectively promote the efficiency and economics of energy storage,centralized shared energy storage(SES)station with multiple energy storage batteries is developed to enable energy trading among a group of entities.In this paper,we propose the optimal operation with dynamic partitioning strategy for the centralized SES station,considering the day-ahead demands of large-scale renewable energy power plants.We implement a multi-entity cooperative optimization operation model based on Nash bargaining theory.This model is decomposed into two subproblems:the operation profit maximization problem with energy trading and the leasing payment bargaining problem.The distributed alternating direction multiplier method(ADMM)is employed to address the subproblems separately.Simulations reveal that the optimal operation with a dynamic partitioning strategy improves the tracking of planned output of renewable energy entities,enhances the actual utilization rate of energy storage,and increases the profits of each participating entity.The results confirm the practicality and effectiveness of the strategy.

球化和再结晶工艺协同改善脆性CoCrNi_(2)(V_(3)B_(2)Si)_(0.2)共晶高熵合金强韧性研究

共晶高熵合金因其所展现出的特殊物理/化学性能而受到材料界的广泛关注.然而,除了FCC+B2系外,其他大部分所报道的共晶高熵合金系均呈现为固有脆性,这使得它们逐渐失去了工业应用的竞争优势.本工作提出了一种新的协同改善脆性CoCrNi_(2)(V_(3)B_(2)Si)_(0.2)共晶高熵合金强韧性的球化+再结晶工艺.基于该工艺,CoCrNi_(2)(V_(3)B_(2)Si)_(0.2)共晶高熵合金获得了一种精心设计的球状M3B2镶嵌在细小FCC晶界处的微观结构,使其抗拉强度、屈服强度和延伸率从923 MPa/620 MPa/1.3%提高到1326-1530 MPa/990-1330 MPa/8.4%-13.9%,提高了43.7%-65.8%/59.7%-114.5%/546%-9.69%.强塑化机制主要归因于球化和再结晶微观结构提供了更多的界面阻碍位错移动和促进了更加均匀的应力分布.该工作为协同改善脆性共晶高熵合金强韧性以满足工程安全性需求提供了新的思路.

Regional source contributions to fine particulate matter of less studied cities in Beijing-Tianjin-Hebei region in 2017

Located in the central area of the North China Plain, the Beijing-Tianjin-Hebei region experiences severe air pollution, especially for Hebei province with five cities among the top 10 cities with the worst air quality nationwide. However, less studies have investigated local and regional contributions to cities in Hebei in comparison to Beijing and Tianjin. In this study, a source-oriented version of the Community Multiscale Air Quality (CMAQ) model is used to quantify inter- and intra-province transport of PM_(2.5) in 11 cities within Hebei in 2017. PM_(2.5) and its components vary seasonally with high levels in winter and low levels in summer. Local contributions to total PM_(2.5) within cities are 23%–61%, while intra-province transport contributes 10%–30%, and inter-province transport contributes 23%–46%, with top contributors of Shandong (4%–22%), Henan (3%–15%) and Shanxi (2%–11%). The primary components in most cities are mainly from local sources (30%–80%), and the secondary components are mainly from inter-province contributions (30%–66%). Local sources have the highest contributions to total PM_(2.5) in Shijiazhuang (∼49%), followed by Shanxi (∼12%) and Xingtai (∼7%). Secondary components from local and intra-province emissions are the major cause of heavy pollution events. These results suggest that both local and regional joint control measures with neighboring cities and provinces are necessary for pollution reduction in Hebei cities.

大气氧化性及其对二次污染物形成的贡献

大气氧化性(atmospheric oxidation capacity,AOC)是指大气化学过程对一次污染物的氧化能力,一般用氧化剂的浓度或者总反应速率来表征.AOC对二次污染的形成起重要的作用,是研究二次污染物的重要指标.本研究利用三维空气质量模式(Community Multi-scale Air Quality model,CMAQ)模拟了2013和2020年我国主要大气氧化剂(HO_(2)、OH和NO_(3)自由基)与二次污染物(臭氧和二次颗粒物)及其前体物,并结合观测数据,综合讨论了AOC对二次污染物生成的作用.结果表明,从2013~2020年,颗粒物浓度显著下降,而AOC水平并未明显降低,甚至在华北平原(NCP)和珠江三角洲(PRD)地区还略有上升.主要氧化剂浓度呈现一定的区域特征,HO_(x)(OH+HO_(2))在四川盆地浓度水平较高,而NO_(3)自由基在华北平原的浓度水平较高.O_(3)光解过程是生成AOC最主要的来源,而OH自由基和二氧化氮生成硝酸的过程是AOC最主要的汇.AOC与二次污染物浓度关系密切,在O_(3)重污染情况下,AOC的浓度水平与年均值相比增幅明显(最高可达475%);同时,在高AOC水平下,二次有机气溶胶(secondary organic aerosol,SOA)的浓度也有显著升高.因为二次无机气溶胶(secondary inorganic aerosol,SIA)的生成过程包含气相和其他相位过程,并且受排放的季节性变化影响较大,因此总浓度与AOC关系不直接.此外,通过情景模拟分析了AOC和二次污染物浓度对不同污染物减排的响应.在夏季,减少氮氧化物(NO_(x))排放可以降低AOC,而在冬季AOC对排放调整的响应较为复杂,呈现较强的区域差异性特征.研究表明,在减少一次排放的同时要控制AOC的升高,才能高效地实现空气质量的提升和细颗粒物(PM_(2.5))与O_(3)协同控制的目标.

支气管哮喘差异表达基因的筛选和生物信息学分析

目的筛选支气管哮喘核心差异表达基因并对其进行生物信息学分析。方法从基因表达数据库(GEO)下载哮喘患者巨噬细胞基因芯片数据GSE22528,该数据集包括了10份人肺泡灌洗液的转录组信息,其中哮喘患者和对照个体各5份。采用R 4.0.4软件筛选差异表达基因(DEGs)。利用DAVID 6.8数据库对筛选出的DEGs进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析。利用STRING在线数据库对DEGs编码蛋白构建蛋白互作网络(PPI),采用Cytoscape软件构建核心模块并确定核心DEGs。结果肺泡灌洗液标本均来自加拿大白种人,哮喘患者和对照个体年龄范围分别为20~37和18~36岁,各组男性均为3例。哮喘患者上调基因449个,下调基因47个。GO分析显示:哮喘患者上调基因主要涉及对未折叠蛋白的反应等生物过程,分子功能集中于未折叠蛋白和生长因子的绑定;下调基因主要涉及组蛋白脱乙酰作用和泛素介导的蛋白质降解等生物过程,分子功能集中于组蛋白脱乙酰酶活性。KEGG通路富集分析显示:通路主要由上调基因富集,涉及Hippo信号通路、肥厚型心肌病、雌激素信号通路、致心律失常性右心室心肌病、基底细胞癌、神经活化的受体配体相互作用、扩张型心肌病和黏附连接等信号通路。PPI分析共得到两个核心模块,筛选出14个核心DEGs,分别为促黑色素聚集激素(PMCH)、孤啡肽前体(PNOC)、鞘氨醇-1-磷酸受体2(S1PR2)、鞘氨醇-1-磷酸受体5(S1PR5)、CC型趋化因子配体21(CCL21)、Kelch样蛋白25(KLHL25)、泛素结合酶E2V2(UBE2V2)、F-box蛋白17(FBXO17)、味觉受体2型成员3(TAS2R3)、生长抑素受体2(SSTR2)、代谢型谷氨酸受体2(GRM2)、李斯特E3泛素蛋白连接酶1(LTN1)、LIM域特有蛋白7(LMO7)和环指蛋白19A基因(RNF19A),其中LTN1和UBE2V2下调,其余均上调。结论哮喘患者与对照个体存在DEGs、PMCH、PNOC、S1PR2、S1PR5和CCL21基因等可能为哮喘发病机制中的核心基因。

Short-range ordering and its effects on mechanical properties of high-entropy alloys

In this letter,we briefly summarize experimental and theoretical findings of fo rmation and characterization of short-range orderings(SROs)as well as their effects on the defo rmation behavior of high-entropy alloys(HEAs).We show that existence of SROs is a common yet key structural feature of HEAs,and tuning the degree of SROs is an effective way for optimizing mechanical properties of HEAs.In additional,the challenges concerning about formation mechanism and characterization of SROs in HEAs are discussed,and future research activities in this regard are also proposed.

Bioactive skin-mimicking hydrogel band-aids for diabetic wound healing and infectious skin incision treatment

The treatment of diabetic chronic wounds remains a global challenge due to the up-regulated inflammation response,oxidant stress,and persistent infection during healing process.Developing wound dressing materials with ideal biocompatibility,adequate mechanical strength,considerable under-water adhesion,sufficient anti-inflammation,antioxidant,and antibacterial properties is on-demand for clinical applications.In this study,we developed a bioactive skin-mimicking hydrogel band-aid through the combination of tannic acid(TA)and imidazolidinyl urea reinforced polyurethane(PMI)(TAP hydrogel)and explored its potentials in various medical applications,including hemostasis,normal skin incision,full-thickness skin wounds,and bacterial-infection skin incision on diabetic mice.TA was loaded into PMI hydrogel network to enhance the mechanical properties of TAP hydrogels through multiple non-covalent interactions(break strength:0.28-0.64 MPa;elongation at break:650-930%),which could resist the local stress and maintain the structural integrity of wound dressings during applications.Moreover,owing to the promising moisture-resistant adhesiveness and organ hemostasis,outstanding anti-inflammation,antibacterial,and antioxidant properties,TAP hydrogels could efficiently promote the recovery of skin incision and defects on diabetic mice.To further simulate the practical situation and explore the potential in clinical application,we also verified the treatment efficiency of TAP hydrogel in S.aureus-infected skin incision model on diabetic mice.

Hierarchical Community Detection Based on Partial Matrix Convergence Using Random Walks

Random walks are a standard tool for modeling the spreading process in social and biological systems But in the face of large-scale networks, to achieve convergence, iterative calculation of the transition matrix in random walk methods consumes a lot of time. In this paper, we propose a three-stage hierarchical community detection algorithm based on Partial Matrix Approximation Convergence （PMAC） using random walks. First, this algorithm identifies the initial core nodes in a network by classical measurement and then utilizes the error function of the partial transition matrix convergence of the core nodes to determine the number of random walks steps. As such, the PMAC of the core nodes replaces the final convergence of all the nodes in the whole matrix. Finally based on the approximation convergence transition matrix, we cluster the communities around core nodes and use a closeness index to merge two communities. By recursively repeating the process, a dendrogram of the communities is eventually constructed. We validated the performance of the PMAC by comparing its results with those of two representative methods for three real-world networks with different scales

Kinetics of Catalyzed Thermal Degradation of Polylactide and Its Application as Sacrificial Templates

Polylactide(PLA)is an outstanding sacrificial template material for the manufacture of microchannels in a thermosetting matrix.However,the initial thermal degradation temperature of pure PLA is relatively high(about 280℃),which limits its use as a sacrificial template.In this report,we found that TBD,an organic base catalyst,can significantly reduce the thermal degradation temperature of PLA.TBD has higher catalytic activity for the thermal degradation of PLA compared with Tin(II)oxalate(Sn(Oxa)),one catalyst reported in the literature.Moreover,the gaseous products catalyzed by TBD for PLA thermal degradation are mainly lactide,and the formation temperature of the monomer is lower and the yield is higher,which may have potential value for PLA recycling.A combined catalyst,S8T2,was composed of 80%low activity catalyst Sn(Oxa)and 20%high activity catalyst TBD,which can catalyze the rapid degradation of PLA without greatly damaging the mechanical properties of PLA.PLA-S8T2 sacrificial fibers can form high-precision one-dimensional microchannels in the epoxy resin matrix,and 3D-printed PLA-S8T2 sacrificial templates can be used to form three-dimensional microchannels in a thermosetting matrix by vaporization of sacrificial components process(VaSC).These features highlight the great potential of PLA-S8T2 as sacrificial template material for the preparation of the complicated microchannels in the thermosetting matrix.

Self-generating nanogaps for highly effective surface-enhanced Raman spectroscopy

The fabrication of surface enhanced Raman spectroscopy(SERS)substrates with controlled high density hot spots still remains challenging.Herein,we report highly effective SERS substrates containing the self-generating(SG)nanogaps from polystyrene nanosphere monolayer through isotropic plasma etching.The emergence of multimode hot spots,i.e.,metal film over nanosphere(MFON)-like hot spots(closed gaps,0 nm),individual self-aligned hot spots(discrete gaps,>20 nm)and threedimensional(3D)hot spots(nanogaps,1-10 nm),makes the SG SERS substrates superior as compared to the traditional MFON or the well-ordered self-aligned SERS substrates in terms of enhancement,uniformity,and reproducibility.The SG SERS substrates can function as the excellent SERS platforms for trace molecule detection in the practical application fields.

A ratiometric fluorescent nanoprobe based on ZIF-8@AuNCs-Tb for visual detection of 2,6-pyridinedicarboxylic acid

The rapid and sensitive detection of 2,6-pyridinedicarboxylic acid(DPA),one of the main biomarkers of Bacillus anthracis,is of great significance for the screening and diagnosing of anthrax.Herein,a ratiometric fluorescent nanoprobe based on zeolite imidazolate framework-8(ZIF-8)@AuNCs-Tb was constructed by embedding both gold nanoclusters(AuNCs)and terbium ions(Tb^(3+))into ZIF-8 for highresolution visual detection of DPA.Due to the aggregation induced emission enhancement(AIE)effect,AuNCs embedded in ZIF-8 emit a strong orange fluorescence.When Tb^(3+)is coordinated with DPA added to the nanoprobe,it will emit a strong green fluorescence owing to the antenna effect.The results reveal that ZIF-8@AuNCs-Tb nanoprobe can detect DPA effectively with a good linear relationship in the range of 40-200 and 200-1000μmol/L,the limit of detection(LOD)is estimated at 1.8μmol/L(3σ/k).The proposed nanoprobe shows a remarkable selectivity for DPA and is quite easy to realize visualization based on the fluorescent color changing from orange to green,which has potential application in clinical diagnosis.The feasibility of this method was verified by standard addition recovery experiments simulating the release of DPA from spores.