Nonreciprocal transport in the superconducting state of the chiral crystal NbGe_(2)

Due to the lack of inversion,mirror or other roto-inversion symmetries,chiral crystals possess a well-defined handedness which,when combined with time-reversal symmetry breaking from the application of magnetic fields,can give rise to directional dichroism of the electrical transport phenomena via the magnetochiral anisotropy.In this study,we investigate the nonreciprocal magneto-transport in microdevices of NbGe_(2),a superconductor with structural chirality.A giant nonreciprocal signal from vortex motions is observed during the superconducting transition,with the ratio of nonreciprocal resistance to the normal resistanceγreaching 6×10^(5)T^(-1)·A^(-1).Interestingly,the intensity can be adjusted and even sign-reversed by varying the current,the temperature,and the crystalline orientation.Our findings illustrate intricate vortex dynamics and offer ways of manipulation on the rectification effect in superconductors with structural chirality.

Crystal growth, magnetic and electrical transport properties of the kagome magnet RCr_(6)Ge_(6)(R= Gd–Tm)

Kagome magnets have attracted considerable research attention due to the interplay between topology,magnetism and electronic correlations.In this study we report single-crystal synthesis of a series of the kagome magnets.RCr_(6)Ge_(6)(R=Gd-Tm) that possess defect-free Cr kagome lattices and systematically study their magnetic and electrical transport properties.The transition from a canted ferrimagnetic to a paramagnetic state in GdCr_(6)Ge_(6),TbCr_(6)Ge_(6),DyCr_(6)Ge_(6),HoCr_(6)Ge_(6),ErCr_(6)Ge_(6) and TmCr_(6)Ge_(6) occurs at 11.3 K,10.8 K,4.3 K,2.5 K,3.3 K and below 2 K,respectively,due to R-R interactions within the compounds.Magnetization measurements reveal highly anisotropic magnetism with canted magnetic moments in these compounds.In electrical transport,both negative and positive magnetoresistances at different magnetic fields and temperatures have been observed due to the competition between different scattering mechanisms.This work enriches our understanding of the Cr-based kagome magnets and paves the way to search for possible topological responses in this family.

Complex heterozygous mutations in hereditary spherocytosis:A case report

BACKGROUND The aim of this study was to investigate the complex heterozygous mutations of ANK1 and SPTA1 in the same individual and improve our understanding of hereditary spherocytosis(HS)in children.We also hope to promote the application of gene detection technology in children with HS,with the goals of identifying more related gene mutations,supporting the acquisition of improved molecular genetic information to further reveal the pathogenesis of HS in children,and providing important guidance for the diagnosis,treatment,and prevention of HS in children.CASE SUMMARY A 1-year and 5-month-old patient presented jaundice during the neonatal period,mild anemia 8 months later,splenic enlargement at 1 year and 5 months,and brittle red blood cell permeability.Genetic testing was performed on the patient,their parents,and sister.Swiss Model software was used to predict the protein structure of complex heterozygous mutations in ANK1 and SPTA1.Genetic testing revealed that the patient harbored a new mutation in the ANK1 gene from the father and a mutation in the SPTA1 gene from the mother.Combined with the clinical symptoms of the children,it is suggested that the newly discovered complex heterozygous mutations of ANK1 and SPTA1 may be the cause,providing important guidance for revealing the pathogenesis,diagnosis,treatment,and promotion of gene detection technology in children with HS.CONCLUSION This case involves an unreported complex heterozygous mutation of ANK1 and SPTA1,which provides a reference for exploring HS.

松辽盆地深层中-基性火山岩有利相带及储层“甜点”逐级识别

松辽盆地深层下白垩统火石岭组中-基性火山岩广泛发育,岩性-岩相复杂,储层横向非均质性强,储层“甜点”识别难,制约了油气勘探和开发。以查干花地区为例,研究了松辽盆地深层中-基性火山岩岩相及储层特征,提出了火山通道、有利岩相、有利岩性及储层甜点识别方法。研究表明:①火山岩岩相主要为3相6亚相,优质储层岩性主要为粗粒、细粒凝灰岩和凝灰质砂岩,在近-中源相带中火山爆发相储层最为发育,相带控制了有利岩性凝灰岩的分布区域,物性和含气性是火山岩“甜点”的主要控制因素。②通过分析每个喷发期次的裂缝密度,结合蚂蚁体与地震数据的叠合剖面,识别出了不同喷发期次的火山通道,以判断火山近源相带,再结合火山机构厚度和能量半衰时属性划分中源与远源相带的边界,实现储层“甜点”发育区的定性刻画。③为降低地震反演定量预测中-基性火山岩储层的多解性,优选出低波阻抗表征沉凝灰岩,低密度表征高核磁孔隙度储层,低拉梅常数表征气层,基于此,在叠前反演密度预测孔隙度数据体的基础上,逐级剔除干扰沉凝灰岩、低孔隙度层及非气层,得到较为可靠的火山岩储层“甜点”空间分布预测结果。经验证,预测结果符合有利相带分布规律,且与已钻井气层解释结论吻合。应用有利相带定性认识及逐级定量刻画“甜点”空间分布,部署井取得较好效果,该方法可在深层火山岩“甜点”分布地震预测中广泛推广。

Timing and structural controls on skarn-type and vein-type mineralization at the Xitian tin-polymetallic deposit, Hunan Province, SE China

Xitian tin-polymetallic deposit, located in the eastern Hunan Province, SE China, hosts quartz vein and skarn in the contact zone between carbonate and two stages granites. Critical geodynamic questions for South China are whether different types of mineralization form in the same time and how the magmatism–tectonic system controls the ore-forming process. Based on the distribution of the orebodies, six cassiterite samples from different types of mineralization are collected for dating. In-situ LA-MCICP-MS U–Pb isotopic data yielded concordia low intercept ages between 154 and 157 Ma, indicating that different types of mineralization belong to the same magmatism–mineralization system. Coupled with the study of the kinematic indicators, it suggests that the structural control of the wall rocks constrain the types of mineralization. These results provide further evidence of a close temporal link between the structure and the tin-polymetallic mineralization in Xitian deposit. Considering the structure in the district, granite dome plays an important role in the ore-forming process. The age and structural signatures in Xitian deposit are the response to the subduction of Pacific Plate.

Tumor shrinkage by cyclopamine tartrate through inhibiting hedgehog signaling

The link of hedgehog (Hh) signaling activation to human cancer and synthesis of a variety of Hh signaling inhibitors raise great expectation that inhibiting Hh signaling may be effective in human cancer treatment. Cyclopamine (Cyc), an alkaloid from the Veratrum plant, is a specific natural product inhibitor of the Hh pathway that acts by targeting smoothened (SMO) protein. However, its poor solubility, acid sensitivity, and weak potency relative to other Hh antagonists prevent the clinical development of Cyc as a therapeutic agent. Here, we report properties of cyclopamine tartrate salt (CycT) and its activities in Hh signaling-mediated cancer in vitro and in vivo. Unlike Cyc, CycT is water soluble (5-10 mg/mL). The median lethal dose (LD50) of CycT was 62.5 mg/kg body weight compared to 43.5 mg/kg for Cyc, and the plasma half-life (T1/2) of CycT was not significantly different from that of Cyc. We showed that CycT had a higher inhibitory activity for Hh signaling-dependent motor neuron differentiation than did Cyc (IC50 = 50 nmol/L for CycT vs. 300 nmol/L for Cyc). We also tested the antitumor effectiveness of these Hh inhibitors using two mouse models of basal cell carcinomas (K14cre:Ptch1neo/neo and K14cre:SmoM2YFP). After topical application of CycT or Cyc daily for 21 days, we found that all CycT-treated mice had tumor shrinkage and decreased expression of Hh target genes. Taken together, we found that CycT is an effective inhibitor of Hh signaling-mediated carcinogenesis.

Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism

BACKGROUND Nonalcoholic fatty liver disease (NAFLD) is a global metabolism-associated liver disease.Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a newly discovered secreted protein that is involved in metabolic homeostasis.However,much remains to be discovered about its function in hepatic lipid metabolism;thus,we assessed whether MANF could regulate hepatic metabolism.AIM To establish in vivo and in vitro NAFLD models to explore the role of MANF in hepatic lipid metabolism.METHODS HepG2 cells treated with free fatty acids (FFAs) and ob/ob mice were used as NAFLD models.Liver tissues collected from wild type and ob/ob mice were used to detect MANF expression.Cells were treated with FFAs for different durations.Moreover,we used lentiviral constructs to establish overexpression and knockdown cell models in order to interfere with MANF expression levels and observe whether MANF influences hepatic steatosis.Western blot analysis and quantitative real-time PCR were used to detect protein and gene expression,and oil red O staining was used to visualize intracellular lipid droplets.RESULTS Hepatic MANF protein and mRNA expression in wild type mice were 10-fold and 2-fold higher,respectively,than those in ob/ob mice.The MANF protein was temporarily increased by 1.3-fold after stimulation with FFAs for 24 h and gradually decreased to 0.66-fold that of the control at the 72 h time point in HepG2 cells.MANF deficiency upregulated the expression of genes involved infatty acid synthesis,cholesterol synthesis,and fatty acid uptake and aggravated HepG2 cell steatosis,while MANF overexpression inhibited fatty acid synthesis and uptake and cholesterol synthesis,and rescued HepG2 cells from FFAsinduced steatosis.Furthermore,a significant decrease in triglyceride levels was observed in the MANF overexpression group compared with the control group(0.4288±0.0081 mmol/g vs 0.3746±0.0121 mmol/g,P <0.05) upon FF As treatment.There was also a 17%decrease in intracellular total cholesterol levels between the MANF overexpression group and the control group (0.1301±0.0059mmol/g vs 0.1088±0.0009 mmol/g,P <0.05) upon FF As treatment.Moreover,MANF suppressed lipid deposition in HepG2 cells.CONCLUSION Our findings indicate that MANF improves the phenotype of liver cell steatosis and may be a potential therapeutic target in hepatic steatosis processes.

Using the theory of coevolution to predict protein-protein interactions in non-small cell lung cancer

Systems biology has become an effective approach for understanding the molecular mechanisms underlying the development of lung cancer.In this study,sequences of 100 non-small cell lung cancer (NSCLC)-related proteins were downloaded from the National Center for Biotechnology Information (NCBI) databases.The Theory of Coevolution was then used to build a protein-protein interaction (PPI) network of NSCLC.Adopting the reverse thinking approach,we analyzed the NSCLC proteins one at a time.Fifteen key proteins were identified and categorized into a special protein family F(K),which included Cyclin D1 (CCND1),E-cadherin (CDH1),Cyclin-dependent kinase inhibitor 2A (CDKN2A),chemokine (C-X-C motif) ligand 12 (CXCL12),epidermal growth factor (EGF),epidermal growth factor receptor (EGFR),TNF receptor superfamily,member 6(FAS),FK506 binding protein 12-rapamycin associated protein 1 (FRAP1),O-6-methylguanine-DNA methyltransferase (MGMT),parkinson protein 2,E3 ubiquitin protein ligase (PARK2),phosphatase and tensin homolog (PTEN),calcium channel voltage-dependent alpha 2/delta subunit 2 (CACNA2D2),tubulin beta class I (TUBB),SWI/SNF-related,matrix-associated,actin-dependent regulator of chromatin,subfamily a,member 2 (SMARCA2),and wingless-type MMTV integration site family,member 7A (WNT7A).Seven key nodes of the sub-network were identified,which included PARK2,WNT7A,SMARCA2,FRAP1,CDKN2A,CCND1,and EGFR.The PPI predictions of EGFR-EGF,PARK2-FAS,PTEN-FAS,and CACNA2D2-CDH1 were confirmed experimentally by retrieving the Biological General Repository for Interaction Datasets (BioGRID) and PubMed databases.We proposed that the 7 proteins could serve as potential diagnostic molecular markers for NSCLC.In accordance with the developmental mode of lung cancer established by Sekine et al.,we assumed that the occurrence and development of lung cancer were linked not only to gene loss in the 3p region (WNT7A,3p25) and genetic mutations in the 9p region but also to similar events in the regions of 1p36.2 (FRAP1),6q25.2-q27 (PARK2),and 11q13 (CCND1).Lastly,the invasion or metastasis of lung cancer happened.

Desulfurization of gasoline by condensation of thiophenes with formaldehyde in a biphasic system using aqueous phase of acids

Aqueous phase of acids as catalysts for the desulfurization of gasoline by condensation of thiophenes with form- aldehyde in a biphasic system was investigated. Two types of model gasoline with and without aromatics and olefins were employed in this work. The desulfurization rates were above 90% on these two types of model gasoline using formic acid and HaPW12O40 （0.8 mol·L-1）, indicating that the presence of aromatics and olefins has no effect on the desulfurization rate. High temperature （above 90 ℃） was more favorable to the process for desulfurization. Four hours was considered to be the proper treating time for the sulfur removal. In addition, aqueous phase of acids could be recycled at least 4 times without decreasing desulfurization rate. Finally, the possible process for the integration of condensation desulfurization into the existing refinery process for the production of gasoline with low sulfur content was proposed.

基于叠前方位各向异性的火山岩裂缝预测——以松辽盆地南部LFS地区为例

利用叠前方位道集地震数据进行各向异性参数反演是目前裂缝预测的主要方法之一,其中RÜGER近似方程和傅里叶级数展开式2种算法应用较为广泛,RÜGER近似方程中的各向异性梯度与傅里叶级数展开式的二阶项均可以表征裂缝强度。应用2种方程在单层界面、实钻井裂缝层分别对比了计算方法的适用性,并在实际火山岩发育区对比了裂缝空间预测结果。单界面模型2种方程预测裂缝强度存在量纲差异,RÜGER近似方程裂缝强度值域大于傅里叶级数展开式计算结果,RÜGER近似方程计算裂缝方位存在多解性,可能为垂直裂缝方向;井上裂缝层应用2种方法计算裂缝方位和强度结果基本一致;在松辽盆地南部LFS地区火山岩地层应用2种方法分别预测裂缝发育情况,傅里叶级数二阶项相比方位各向异性梯度,与电成像测井解释裂缝强度吻合稍好,且预测裂缝方位与成像测井解释方位相同。研究认为,在火山岩领域傅里叶级数方程预测裂缝方法更适于推广应用。

Reconstruction of a muon bundle in the JUNO central detector

The Jiangmen Underground Neutrino Observatory(JUNO)is a multi-purpose neutrino experiment.One of the main goals is to determine the neutrino mass ordering by precisely measuring the energy spectrum of reactor antineutrinos.For the detection of reactor antineutrinos,cosmogenic backgrounds,such as^(9)Li/^(8)He and fast neutrons induced by cosmic muons,should be rejected carefully by applying muon veto cuts,which require good muon track reconstruction.With a 20-kton liquid scintillator detector,the simulation shows the proportion of muon bundles(muon multiplicity≥2)to be approximately 8%in JUNO,whereas its reconstruction has been rarely discussed in previous experiments.This study proposes an efficient algorithm for muon track reconstruction based on the charge response of a photomultiplier tube array.This is the first reconstruction of muon bundles in a large-volume liquid scintillator detector.In addition,the algorithm shows good performance and potential for reconstruction for both a single muon and double muons(muon multiplicity=2).The spatial resolution of a single-muon reconstruction was20 cm,and the angular resolution was 0.5°.For doublemuon reconstruction,the spatial and angular resolutions could be 30 cm and 1.0°,respectively.Moreover,this paper also discusses muon classification and the veto strategy.

Manipulating the ion-transference and deposition kinetics by regulating the surface chemistry of zinc metal anodes for rechargeable zinc-air batteries

Aqueous zinc-air battery(ZAB)has attractive features as the potential energy storage system such as high safety,low cost and good environmental compatibility.However,the issue of dendrite growth on zinc metal anodes has seriously hindered the development of ZAB.Herein,the N-doped carbon cloth(NC)prepared via magnetron sputtering is explored as the substrate to induce the uniform nucleation of zinc metal and suppress dendrite growth.Results show that the introduction of heteroatoms accelerates the migration and deposition kinetics of Zn^(2+)by boosting the desolvation process of Zn^(2+),eventually reducing the nucleation overpotential.Besides,theoretical calculation results confirm the zincophilicity of N-containing functional group(such as pyridine N and pyrrole N),which can guide the nucleation and growth of zinc uniformly on the electrode surface by both promoting the redistribution of Zn^(2+) in the vicinity of the surface and enhancing its interaction with zinc atoms.As a result,the half-cell assembled with magnetron sputtered carbon cloth achieves a high zinc stripping/plating coulombic efficiency of 98.8%and long-term stability of over 500 cycles at 0.2 mA cm^(-2).And the Coulombic efficiency reached about 99.5%at the 10th cycle and maintained for more than 210 cycles at a high current density of 5.0 mA cm^(-2).The assembled symmetrical battery can deliver 220 plating/stripping cycles with ultra-low voltage hysteresis of only 11 mV.In addition,the assembled zinc-air full battery with NC-Zn anode delivers a high special capacity of about 429 mAh g_(Zn)^(-1) and a long life of over 430 cycles.The effectiveness of surface functionalization in promoting the transfer and deposition kinetics of Zn^(2+) presented in this work shows enlightening significance in the development of metal anodes in aqueous electrolytes.

Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variants

Genome-wide association studies(GWASs) are the most widely used method to identify genetic risk loci associated with orofacial clefts(OFC). However, despite the increasing size of cohort, GWASs are still insufficient to detect all the heritability,suggesting there are more associations under the current stringent statistical threshold. In this study, we obtained an integrated epigenomic dataset based on the chromatin conformation of a human oral epithelial cell line(HIOEC) using RNA-seq, ATAC-seq,H3K27ac Ch IP-seq, and DLO Hi-C. Presumably, this epigenomic dataset could reveal the missing functional variants located in the oral epithelial cell active enhancers/promoters along with their risk target genes, despite relatively less-stringent statistical association with OFC. Taken a non-syndromic cleft palate only(NSCPO) GWAS data of the Chinese Han population as an example, 3664 SNPs that cannot reach the strict significance threshold were subjected to this functional identification pipeline.In total, 254 potential risk SNPs residing in active cis-regulatory elements interacting with 1 718 promoters of oral epitheliumexpressed genes were screened. Gapped k-mer machine learning based on enhancers interacting with epithelium-expressed genes along with in vivo and in vitro reporter assays were employed as functional validation. Among all the potential SNPs, we chose and confirmed that the risk alleles of rs560789 and rs174570 reduced the epithelial-specific enhancer activity by preventing the binding of transcription factors related to epithelial development. In summary, we established chromatin conformation datasets of human oral epithelial cells and provided a framework for testing and understanding how regulatory variants impart risk for clefts.

Risk Analysis of Hepatocellular Carcinoma in Northeast China

Objective： It is known that chronic hepatitis B virus （HBV） infection is a main risk factor for hepatocellular carcinoma （HCC）. To assess the effect of HBV infection and its interaction with other factors on the risk for HCC, a hospital-based case-control study was carried out in Northeast China. Methods： A total of 384 cases with hepatocellular carcinoma and 432 controls without evidence of liver diseases were enrolled in the study. Blood samples were collected to detect the serum markers of hepatitis B virus （HBV） and hepatitis C virus （HCV） and questionnaires about lifestyle and family tumor history were performed in all subjects. Results： The total infection rate of HBV in hepatocellular carcinoma cases was 70.8% and 10.0% in non-liver disease controls. There was a statistically significant difference （P〈0.0001） between cases and controls （OR= 22.0; 95%CI：15.0-32.3）. Interaction analysis indicated that in HBV chronic carriers with HCV infection or alcohol consumption or family HCC history, the risk for HCC increased （OR=41.1, 95%CI： 20.2-83.9, OR=125.0, 95%CI： 66.5-235.2; OR=56.9, 95%CI： 27.2-119.3 respectively）. In addition, hepatitis B history, HCV infection, hepatic cirrhosis and family history of HCC were also potential HCC independent risk factors. Conclusion： We confirmed that HBV is a chief risk factor for hepatocellular carcinoma and accounts for 67.7% of all hepatocellular carcinoma in Northeast China. HCV infection, alcohol intake and family history could enhance the risk for HCC in chronic HBV carriers.

Critical behavior in the itinerant ferromagnet SmMn_(2)Ge_(2)

Transition metal and rare earth intermetallics have been a fertile playground for research of various quantum states.We report detailed magnetic studies on Sm Mn_(2)Ge_(2),an anisotropic itinerant magnet with multiple magnetic phases.The critical behavior of the ferromagnetic phase transition is investigated by employing the modified Arrott plot with the Kouvel-Fisher method.The critical temperature TCis determined to be around 342.7 K with critical exponents ofβ=0.417 andγ=1.122,and the interaction function is found to be J(r)~r^(-4.68),suggesting the coexistence of long-range and shortrange magnetic interactions.Our results contribute to the understanding of complex magnetism in Sm Mn_(2)Ge_(2),which may provide fundamental guidance in future spintronic applications.

Spin freezing in the van der Waals material Mn_(2)Ga_(2)S_(5)

Geometrical frustration in low-dimensional magnetic systems has been an intriguing research aspect,where the suppression of conventional magnetic order may lead to exotic ground states such as spin glass or spin liquid.In this work we report the synthesis and magnetism study of the monocrystalline Mn_(2)Ga_(2)S_(5),featuring both the van derWaals structure and a bilayered triangular Mn lattice.Magnetic susceptibility reveals a significant antiferromagnetic interaction with a Curie-Weiss temperature θ_(w)~-260 K and a high spin S=5/2 Mn^(2+) state.However,no long range magnetic order has been found down to 2 K,and a spin freezing transition is found to occur at around 12 K well below its θ_(w).This yields a frustration index of f=-θ_(w)/T_(f)≈22,an indication that the system is highly frustrated.The absence of a double-peak structure in magnetic specific heat compared with the TM_(2)S_(4) compounds implies that the spin freezing behavior in Mn_(2)Ga_(2)S_(5)is a result of the competition between exchange interactions and the 2D crystalline structure.Our results suggest that the layered Mn_(2)Ga_(2)S_(5)would be an excellent candidate for investigating the physics of 2D magnetism and spin disordered state.

Efficacy of PC-PRL implantation in the treatment of high myopia and its complications

Objective: To study the effect of implantable posterior chamber phakic refractive lens (PC-PRL) in the treatment of high myopia and its effect on naked eye vision and retinal complications. Methods: A total of 110 patients (220 eyes) with high myopia admitted to our hospital from June 2017 to February 2019 were selected as subjects. All patients were treated with PC-PRL implantation. The UCVA, BCVA, intraocular pressure, corneal endothelial cell count and intraocular lens position (anterior chamber depth and arch height) were compared before and after the treatment. Results: 6 months after treatment, 68.18% of patients had UCVA between 0.6 and 0.9, 31.81% of patients had UCVA of more than 1.0, and 100.00% of patients had BCVA of more than1.0. The BCVA and UCVA values of patients at 1 week, 1 month, 3 months and 6 months after operation were significantly greater than those before surgery (P<0.05). There were no significant differences in BCVA and UCVA among 1 week, 1 month, 3 months and 6 months after operation (P>0.05). The intraocular pressure was significantly greater than that of the preoperative one week after operation (P<0.05). There was no significant difference between the intraocular pressure at 1 month, 3 months and 6 months after operation (P>0.05). There was no significant difference in corneal endothelial cell count and arch height before and after treatment (P>0.05). The depth of anterior chamber at 3 months and 6 months after operation was significantly less than that before operation (P<0.05). There was no significant difference in the anterior chamber depth between 3 months and 6 months after operation (P<0.05). There were 7 cases of corneal edema, 4 cases of high pressure eyes, 2 cases of refractive regression within 6 months, and 1 case of near-difficulty within 1 month. The total complication rate was 12.73%. Conclusion: PC-PRL implantation is effective in the treatment of high myopia, which can improve the naked eye and correct vision. At the same time, the incidence of retinal complications is low, which can be promoted and applied clinically.

Correlation between the expressions of metastasis-associated factor-1 in colon cancer and vacuolar ATP synthase

BACKGROUND Clinical prognosis often worsens due to high recurrence rates following radical surgery for colon cancer.The examination of high-risk recurrence factors post-surgery provides critical insights for disease evaluation and treatment planning.AIM To explore the relationship between metastasis-associated factor-1 in colon cancer(MACC1)and vacuolar ATP synthase(V-ATPase)expression in colon cancer tissues,and recurrence rate in patients undergoing radical colon cancer surgery.METHODS We selected 104 patients treated with radical colon cancer surgery at our hospital from January 2018 to June 2021.Immunohistochemical staining was utilized to assess the expression levels of MACC1 and V-ATPase in these patients.RESULTS The rates of MACC1 and V-ATPase positivity were 64.42%and 67.31%,respe-ctively,in colon cancer tissues,which were significantly higher than in paracan-cerous tissues(P<0.05).Among patients with TNM stage III,medium to low differentiation,and lymph node metastasis,the positive rates of MACC1 and V-ATPase were significantly elevated in comparison to patients with TNM stage I-II,high differentiation,and no lymph node metastasis(P<0.05).The rate of MACC1 positivity was 76.67%in patients with tumor diameters>5 cm,notably higher than in patients with tumor diameters≤5 cm(P<0.05).We observed a positive correlation between MACC1 and V-ATPase expression(rs=0.797,P<0.05).The positive rates of MACC1 and V-ATPase were significantly higher in patients with recurrence compared to those without(P<0.05).Logistic regression analysis revealed TNM stage,lymph node metastasis,MACC1 expression,and V-ATPase expression as risk factors for postoperative colon cancer recurrence(OR=6.322,3.435,2.683,and 2.421;P<0.05).CONCLUSION The upregulated expression of MACC1 and V-ATPase in colon cancer patients appears to correlate with clinicopathological features and post-radical surgery recurrence.

Effect of TICL implantation on corneal astigmatism and the stability and visual quality after operation

Objective:To explore the effect of TICL implantation on corneal astigmatism,postoperative stability and visual quality.Methods:110 patients(174 eyes)with high myopia and astigmatism who were treated with TICL implantation in our hospital from July 2016 to July 2018 were selected as the research subjects.The follow-up time was 12 months.The changes of UCVA,BCVA,and astigmatism values were used as indicators to evaluate the effectiveness of treatment.The diopter changes of the affected eye at different periods after surgery and the changes in the rotation angle of the intraocular lens axis at different periods after surgery were used as indicators to determine the stability of surgical treatment.Results:The UCVA and BCVA of the affected eyes after operation were significantly higher than those before operation(P<0.05).Compared with pre-operation,the diopter of the affected eyes was significantly reduced after operation(P<0.05);Based on the axial position of the intraocular lens on the first day after surgery,the degree of rotation of the intraocular lens implanted in the affected eyes was significantly reduced(P<0.05);Compared with pre-operation,there was no statistically significant difference in corneal endothelial cell count,intraocular pressure,and arch height after operation.Conclusion:Patients with high myopia and corneal astigmatism received TICL implantation after correction treatment,visual acuity was significantly improved,visual quality was significantly improved,with good postoperative stability,which had good clinical promotion value.

Effect of Aidi injection-assisted intravenous chemotherapy on serum tumor markers and peripheral blood immune molecules in patients with colon cancer

Objective:To discuss the effect of Aidi injection-assisted intravenous chemotherapy on serum tumor markers and peripheral blood immune molecules in patients with colon cancer. Methods: 92 patients with colon cancer who were hospitalized in Deyang People's Hospital in Sichuan Province between October 2013 and October 2016 were collected and divided into the control group (n=50) who received intravenous chemotherapy alone and the observation group (n=42) who received Aidi injection-assisted intravenous chemotherapy after the therapies were reviewed. The differences in serum tumor markers and peripheral blood immune molecule contents before and after treatment were compared between two groups of patients.Results:Before treatment, differences in serum tumor marker levels and peripheral blood immune molecule levels were not statistically significant between two groups of patients. After treatment, serum tumor markers CEA, CA199, CA50, PTN and CCSA-2 levels of observation group were lower than those of control group, peripheral blood Th1/Th2 cytokines IL-2 and IFN-γ levels were higher than those of control group while IL-4 and IL-10 levels were lower than those of control group, and peripheral blood Th17 cytokines IL-17A and IL-22 levels were lower than those of control group.Conclusion: Aidi injection-assisted intravenous chemotherapy can effectively reduce the serum tumor marker contents and optimize the immune molecule levels in patients with colon cancer.