Outcomes of 957 preterm neonatal fundus examinations in a Guangzhou NICU through 2008 to 2011

AIM: To take fundus examination in the preterm neonates to observe the common diseases and report the outcomes in a neonatal intensive care unit (NICU) in Guangzhou between May 2008 and May 2011. METHODS: Fundus examinations were performed with Retcam II in 957 prematures. RESULTS: There were 957 prematures in this study, including 666 males and 291 females, 2 triple births, 152 twins and 803 singletons. During the three years, 86 infants with any stage retinopathy of prematurity (ROP) (9.0%), 123 infants with retinal hemorrhage (12.9%), 10 infants with neonatal fundual jaundice (1.0%) and 3 babies with congenital choroidal coloboma (0.3%) were found. CONCLUSION: Early detection and prompt treatment of ocular disorders in neonates is important to avoid lifelong visual impairment. Examination of the eyes should be performed in the newborn period and at all well-child visits.

Neuroprotective effects of Shaoyao Gancao decoction against excitatory damage in PC12 cells based on the Src-NR2-nNOS pathway

Objective:To explore the neuroprotective effects of the Shaoyao Gancao decoction(SGD)against excitatory damage in PC12 cells and the role of the Src-NR2-nNOS pathway mediation by SGD in regulatingγ-aminobutyric acid(GABA)-glutamate(Glu)homeostasis.Methods: N-Methyl-d-aspartic acid(NMDA)was used to establish a PC12 cell excitability injury model.To investigate the neuroprotective effect of SGD,a cell counting kit-8(CCK-8)assay was used to determine PC12 cell viability,Annexin V/Propidium Iodide(Annexin V/PI)double staining was used to determine PC12 cell apoptosis,and Ca^(2+)concentration was observed using laser confocal microscopy.GABA receptor agonists and antagonists were used to analyze the neuroprotective interactions betweenγ-aminobutyric acid(GABA)and NMDA receptors.Additionally,molecular biology techniques were used to determine mRNA and protein expression in the Src-NR2-nNOS pathway.We analyzed the correlations between the regulatory sites of GABA and NMDA interactions,excitatory neurotoxicity,and brain damage at the molecular level.Results: NMDA excitotoxic injury manifested as a significant decrease in cell activity,increased apoptosis and caspase-3 protein expression,and a significant increase in intracellular Ca^(2+)concentration.Administration of SGD,a GABAA receptor agonist(muscimol),or a GABAB receptor agonist(baclofen)decreased intracellular Ca^(2+)concentrations,attenuated apoptosis,and reversed NMDA-induced upregulation of caspase-3,Src,NMDAR2A,NMDAR2B,and nNOS.Unexpectedly,a GABA_(A)receptor antagonist(bicuculline)and a GABA_(B)receptor antagonist(saclofen)failed to significantly increase excitatory neurotoxicity.Conclusions: Taken together,these results not only provide an experimental basis for SGD administration in the clinical treatment of central nervous system injury diseases,but also suggest that the Src-NR2A-nNOS pathway may be a valuable target in excitotoxicity treatment.

Protective mechanism of Paeoniae Radix Alba against chemical liver injury based on network pharmacology,molecular docking,and in vitro experiments

Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.

A case of herpesviral corneal endotheliitis presenting with corneal endothelial defect

Dear Editor,Corneal endotheliitis,first reported in 1982 as an autoimmune disorder by Khodadoust and Attarzadeh[1],is now recognized to be frequently induced by viral infections,including varicella zoster virus(VZV),cytomegalovirus(CMV)and herpes simplex virus(HSV),that can cause inflammatory and immune-related injuries or direct damages to corneal endothelial cells[2-3].In cases with this type of endotheliitis of viral origin,manifestations may include conjunctival ciliary congestion or mixed congestion,corneal subepithelial bulla,localized corneal edema,keratic precipitates(KP)characteristic to the edema area,puckered posterior elastic lamina,mild anterior chamber reaction,intraocular pressure(IOP)elevation in presence of inflammation in trabecular meshwork,and endothelial decompensation[3-4].Accurate diagnosis of viral corneal endotheliitis is challenging due to its complex presentation,leading to a high misdiagnosis rate.To the best of our knowledge,there was no previous report of viral corneal endotheliitis presenting with corneal endothelial defects in the English literature.

Effect of Dual Trigger In Vitro Fertilization and Intracytoplasmic Sperm Injection During the Gonadotropin-releasing Hormone-Antagonist Cycle on Final Oocyte Maturation and Cumulative Live Birth Rate in Women with Diminished Ovarian Reserve

Objective It is well known that a dual trigger treatment can improve clinical outcomes of in vitro fertilization(IVF)in high or normal ovarian responders.However,it is not clear whether dual triggering also benefits patients with diminished ovarian reserve(DOR).The aim of this study was to investigate whether a dual trigger treatment of gonadotropin-releasing hormone(GnRH)agonist combined with human chorionic gonadotropin(hCG)for final follicular maturation improves the cumulative live birth rate(CLBR)during the GnRH-antagonist cycle in patients with DOR.Methods This retrospective study included patients with DOR who received a GnRH-antagonist protocol during IVF and intracytoplasmic sperm injection(IVF-ICSI)cycles at Peking University People’s Hospital from January 1,2017 through December 31,2017.Oocyte maturation was triggered by GnRH combined with hCG(n=110)or hCG alone(n=71).Embryos were transferred on the third day after oocyte retrieval or during a subsequent freeze-thaw cycle.Patients were followed up for 3 years.Results The dual trigger treatment did not affect CLBR,which is an overall determinant of the success rate of assisted reproductive technology(ART).Women in the dual trigger group had significantly higher rates of fertilization than those in the hCG group(90.1%vs.83.9%,P=0.040).Conclusion Dual trigger with GnRH agonist and hCG did not improve CLBR in patients with DOR,but did slightly improve fertilization rate,oocyte count,and embryo quality.

直流放电控制高速带斜坡锥体气动力的有效性研究

基于直流放电激波重构气动力控制原理,开展了带斜坡锥体模型的高速(Ma=6)气动力控制风洞试验,采用光纤天平技术,考察了模型在两种放电功率(284 W和517 W)下的气动力/力矩变化情况,并采用纹影成像研究了放电对流动拓扑的影响。纹影图像揭示了由于放电热阻塞和马赫数降低引起的波系重构现象,表现为放电诱导压缩波和再附激波弱化、角度减小。天平信号验证了放电使得模型的轴向力、法向力和俯仰力矩减小,放电功率较大时控制效果明显。通过求解带功率密度源项的Navier-Stokes方程模拟放电的加热效应,数值研究了模型气动力随功率密度的变化规律及加热位置对控制能力的影响。研究表明:模型气动力变化率与功率密度呈正相关;当以激励器的上游位置为参考点时,俯仰力矩变化显著;当加热位置靠近斜坡时,控制能力降低。

光纤珐珀应变计应力温度影响实验分析

对 157 nm 激光微加工技术制作的全光纤珐珀应变计进行了实验分析。介绍了光纤 FP 应变计的制作过程与工作原理,并开展了应变计的加载和温度测试试验。实验表明该光纤 FP 应变计粘贴于悬臂梁后,线性度和重复性较好,在温升过程中,由于悬臂梁的热膨胀造成了光纤应变计的热输出。通过采用对称贴片耦合求解方法进行热输出补偿,光纤应变计的热输出减小为补偿前的 2.1%,为变温环境下的高精度测量提供了解决方案。

白内障超声乳化手术中台盼蓝囊膜染色对角膜内皮细胞影响的Meta分析

目的:系统评价白内障超声乳化手术中台盼蓝囊膜染色对角膜内皮细胞的影响。方法:采用Cochrane系统评价的方法,计算机检索中国知网、万方、维普、SinoMed、PubMed、SpringerLink、Clinicalkey、Medline、Cochrane图书馆、Web of Science、OVID、Embase数据库中关于白内障超声乳化手术中使用台盼蓝进行囊膜染色的随机对照试验(RCTs),检索时限均为建库至2019-04,采用Revman5.3、R 3.7对纳入文献进行Meta分析。结果:共纳入8篇RCTs(378例)。结果显示0.02%、0.06%或0.1%浓度台盼蓝囊膜染色组(193例)与未染色或安慰剂染色组(185例)角膜内皮细胞丢失数均无差异[术后1mo内(WMD=-10.47,95%CI:-26.44~5.61,P=0.20);术后1mo后(WMD=-60.72,95%CI:-170.92~49.49,P=0.28)];两组术后1mo角膜内皮六角形细胞丢失比例无差异(WMD=0.50,95%CI:-2.09~3.09,P=0.71);中央角膜厚度(CCT)无差异(WMD=3.10,95%CI:-5.77~11.98,P=0.49);角膜变异系数(CV)无差异(WMD=-1.00,95%CI:-2.86~0.86,P=0.29)。结论:白内障超声乳化手术中应用0.02%、0.06%或0.1%浓度台盼蓝囊膜染色对角膜内皮细胞数量及功能无明显影响。

Improving the denitration performance and K-poisoning resistance of the V_2O_5-WO_3/TiO_2 catalyst by Ce^(4+) and Zr^(4+) co-doping

A series of V2O5‐WO3/TiO2‐ZrO2,V2O5‐WO3/TiO2‐CeO2,and V2O5‐WO3/TiO2‐CeO2‐ZrO2 catalysts were synthesized to improve the selective catalytic reduction(SCR)performance and the K‐poisoning resistance of a V2O5‐WO3/TiO2 catalyst.The physicochemical properties were investigated by using XRD,BET,NH3‐TPD,H2‐TPR,and XPS,and the catalytic performance and K‐poisoning resistance were evaluated via a NH3‐SCR model reaction.Ce^4+and Zr^4+co‐doping were found to enhance the conversion of NOx,and exhibit the best K‐poisoning resistance owing to the largest BET‐specific surface area,pore volume,and total acid site concentration,as well as the minimal effects on the surface acidity and redox ability from K poisoning.The V2O5‐WO3/TiO2‐CeO2‐ZrO2 catalyst also presents outstanding H2O+SO2 tolerance.Finally,the in situ DRIFTS reveals that the NH3‐SCR reaction over the V2O5‐WO3/TiO2‐CeO2‐ZrO2 catalyst follows an L‐H mechanism,and that K poisoning does not change the reaction mechanism.

杆式天平轴向力元件测量梁夹角优化分析

针对天平轴向力受法向力干扰较大的问题,提出了一种竖直梁轴向力元件的改进结构。通过理论分析和有限元仿真,获得了轴向力/法向力作用下的轴向力元件变形情况;对结构进行受力分析,得到法向力作用下的轴向力测量梁的变形特点及变形原因。提出了一种改进的轴向力元件测量梁结构,测量梁与主梁的连接夹角为不等于90°的优化夹角,该夹角通过仿真结果中的法向力对轴向力的干扰输出与轴向力有效输出之比和该夹角的线性函数关系获得。对改进的天平轴向力元件的有限元仿真结果表明:与传统轴向力元件相比,改进结构的轴向力有效输出仅减小2.77%,但法向力对轴向力的干扰输出减小了99.32%。改进的轴向力元件具有良好的抗法向力干扰的效果,适用于具有大升阻比特性的飞行器风洞模型气动力试验要求。

Unique electronic structure of Mg/O co-decorated amorphous carbon nitride enhances the photocatalytic tetracycline hydrochloride degradation

g-C3N4 is a hot visible light photocatalyst. However, the fast recombination of photogenerated electron- hole pairs leads to unsatisfactory photocatalytic efficiencies. In this study, Mg/O co-decorated amorphous carbon nitride (labeled as MgO-CN) with a unique electronic structure was designed and prepared via a combined experimental and theoretical approach. The results showed that the MgO-CN exhibited an increased light absorption ability and promoted charge separation efficiency. The Mg and O co-decoration created a unique structure that could generate localized electrons around O atoms and enhance the reactant activation capacities via the C→O←Mg route. This could dramatically promote the O2 molecule activation on the catalyst surface to generate reactive species (?O2 –/?OH). The optimized MgO-CN exhibited a high photocatalytic activity for the degradation of tetracycline hydrochloride in water, which was five times higher than that of pristine g-C3N4. The present work could provide a new strategy for modifying the electronic structure of g-C3N4 and enhancing its performance for environmental applications.

In-situ polymerization for PPy/g-C_3N_4 composites with enhanced visible light photocatalytic performance

Polypyrrole‐modified graphitic carbon nitride composites(PPy/g‐C3N4)are fabricated using an in‐situ polymerization method to improve the visible light photocatalytic activity of g‐C3N4.The PPy/g‐C3N4 is applied to the photocatalytic degradation of methylene blue(MB)under visible light irradiation.Various characterization techniques are employed to investigate the relationship between the structural properties and photoactivities of the as‐prepared composites.Results show that the specific surface area of the PPy/g‐C3N4 composites increases upon assembly of the amorphous PPy nanoparticles on the g‐C3N4 surface.Owing to the strong conductivity,the PPy can be used as a transition channel for electrons to move onto the g‐C3N4 surface,thus inhibiting the recombination of photogenerated carriers of g‐C3N4 and improving the photocatalytic performance.The elevated light adsorption of PPy/g‐C3N4 composites is attributed to the strong absorption coefficient of PPy.The composite containing 0.75 wt%PPy exhibits a photocatalytic efficiency that is 3 times higher than that of g‐C3N4 in 2 h.Moreover,the degradation kinetics follow a pseudo‐first‐order model.A detailed photocatalytic mechanism is proposed with·OH and·O2-radicals as the main reactive species.The present work provides new insights into the mechanistic understanding of PPy in PPy/g‐C3N4 composites for environmental applications.

Highly Reversible Li–Se Batteries with Ultra-Lightweight N,S-Codoped Graphene Blocking Layer

The desire for practical utilization of rechargeable lithium batteries with high energy density has motivated attempts to develop new electrode materials and battery systems. Here, without additional binders we present a simple vacuum filtration method to synthesize nitrogen and sulfur codoped graphene(N,S-G) blocking layer, which is ultra-lightweight, conductive, and free standing. When the N,S-G membrane was inserted between the catholyte and separator, the lithium–selenium(Li–Se)batteries exhibited a high reversible discharge capacity of 330.7 mAh g^(-1) at 1 C(1 C = 675 mA g^(-1)) after 500 cycles and high rate performance(over 310 mAh g^(-1) at 4 C) even at an active material loading as high as ～5 mg cm^(-2). This excellent performance can be ascribed to homogenous dispersion of the liquid active material in the electrode, good Li^+-ion conductivity, fast electronic transport in the conductive graphene framework, andstrong chemical confinement of polyselenides by nitrogen and sulfur atoms. More importantly, it is a promising strategy for enhancing the energy density of Li–Se batteries by using the catholyte with a lightweight heteroatom doping carbon matrix.

应变计组合对光纤天平校准和试验的影响分析

三分量光纤天平常采用8支应变计组合求解天平的3个分量,光纤应变计组合仍采用类似于电阻天平的惠斯通电桥组合方式,该组合方式没有利用光纤应变计的输出可直接与天平所受气动载荷建立线性函数关系的特点,应变计数量偏多。为验证采用较少光纤应变计进行光纤天平气动力测量的可行性,本文针对三分量光纤天平,设计了不同应变计数量的8种组合方式,分别进行了光纤天平的静态校准和风洞试验来验证不同应变计组合方式光纤天平的性能。结果表明：3支光纤应变计即可实现三分量天平的精确测量;不同应变计组合方式的光纤天平的性能接近,测量的HB-2模型的气动力系数偏差在2%以内,且天平的校准准度和风洞试验重复性精度都能满足低密度风洞试验要求。

Effect of high-voltage discharge non-thermal plasma on g-C_3N_4 in a plasma-photocatalyst system

The synergistic effect of high voltage discharge non‐thermal plasma(NTP)and photocatalysts on contaminant removal has repeatedly confirmed by plenty of researches.Most previous plasma‐photocatalyst synergistic systems focused on the utilization of the ultraviolet light but ignored the visible light generated by high voltage discharge.Graphitic carbon nitride(g‐C3N4),a metal‐free semiconductor that exhibits high chemical stability,can utilize both the ultraviolet and visible light from high voltage discharge.However,the synergistic system of NTP and g‐C3N4 has been researched little.In this paper,the effect of NTP generated by dielectric barrier discharge(DBD)on g‐C3N4 is studied by comparing the photocatalytic activities,the surface physical structure and the surface chemical characteristics of pristine and plasma treated g‐C3N4.Experimental results indicate that the DBD plasma can change the physical structure and the chemical characteristics and to further affect the photocatalytic activity of g‐C3N4.The effect of NTP on g‐C3N4 is associated with the discharge intensity and the discharge time.For a long time scale,the effect of NTP on g‐C3N4 photocatalysts presents a periodic change trend.

Profile of biological characterizations and clinical application of corneal stem/progenitor cells

Corneal stem/progenitor cells are typical adult stem/progenitor cells.The human cornea covers the front of the eyeball,which protects the eye from the outside environment while allowing vision.The location and function demand the cornea to maintain its transparency and to continuously renew its epithelial surface by replacing injured or aged cells through a rapid turnover process in which corneal stem/progenitor cells play an important role.Corneal stem/progenitor cells include mainly corneal epithelial stem cells,corneal endothelial cell progenitors and corneal stromal stem cells.Since the discovery of corneal epithelial stem cells(also known as limbal stem cells)in 1971,an increasing number of markers for corneal stem/progenitor cells have been proposed,but there is no consensus regarding the definitive markers for them.Therefore,the identification,isolation and cultivation of these cells remain challenging without a unified approach.In this review,we systematically introduce the profile of biological characterizations,such as anatomy,characteristics,isolation,cultivation and molecular markers,and clinical applications of the three categories of corneal stem/progenitor cells.

Drug discovery from traditional Chinese herbal medicine using high content imaging technology

Traditional Chinese medicine(TCM) has been widely used in China and other Asia countries for thousands of years to treat or prevent human diseases. Chinese herbal medicine, one of the most important components of TCM, has unique diversities in chemical components, and thus results in a wide range of biological activities. However, pharmaceutical industry is facing a major challenge to develop a large population of novel natural products and drugs, and considerable efforts have not resulted in highvolume of novel drug discovery and productivity. At present, increasing attention has been paid to Chinese herb medicine modernization in combination with the cutting-age technologies of drug discovery, especially the high throughput selection. High content imaging is an image-based high throughput screening method by using automated microscopy and image analysis software to capture and analyze phenotypes at a large scale to investigate multiple biological features simultaneously in the biological complex. Here, we described the pipeline of the state-of-the-art high content imaging technology, summarized the applications of the high content imaging technology in drug discovery from traditional Chinese herbal medicine, and finally discussed the current challenges and future perspectives for development of high throughput image-based screening technology in novel drug research and discovery.

Epidemiological Study of the Development of Visual Acuity in Preschoolers Aged 3 to 6 in the Shenzhen Area

Purpose: To investigate the visual acuity of preschoolers aged 3 to 6 years in Shenzhen of China. Methods:Visual acuity was measured in preschoolers from eight kindergartens in Shenzhen. Results:A total of 1147 children completed the visual acuity test.There were 77 boys and 62 girls aged 3 years,259 and 216 aged 4,193 and 160 aged 5 and 94 and 86 aged 6. The mean visual acuities of children aged 3,4,5,and 6 years were 0.51±0.10, 0.54±0.13, 0.65±0.14, and 0.71±0.17, respectively. The visual acuity was significantly improved with increasing age (P=0.000). Conclusion:For preschoolers aged 3 to 6, the visual acuity continuously develops and improves. Therefore, age should be considered when diagnosing amblyopia in children.

Loganin regulates glycolipid metabolism by influencing intestinal microbiota and AMPK signaling in obese mice

Objective: We aimed to observe the effects of loganin(Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase(AMPK) signaling in obese mice.Methods: A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research(ICR) mice. Body weight was measured weekly and fasting blood glucose(FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol(TC), triglyceride, high-density lipoproteincholesterol, low-density lipoprotein-cholesterol(LDL-C), and free fatty acids(FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.Results: Log significantly decreased the body weight and the FBG in obese mice(P <.05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls(TC: P =.0020;LDL-C: P =.0233;FFA: P =.0127), and the glucose tolerance of animals was significantly improved(P =.0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPKa), Sirtuin 1(SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha(PGC1a) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.Conclusions: Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.

Spatiotemporal characteristics of sea ice transport in the Baffin Bay and its association with atmospheric variability

Sea ice export through the Baffin Bay plays a vital role in modulating the sea ice cover variability in the Labrador Sea.In this study,satellite-derived sea ice products are used to obtain the sea ice area flux(SIAF)through the three passages in the Baffin Bay(referred to as A,B,and C for the north,middle,and south passages,respectively).The spatial variability of the monthly sea ice drift in the Baffin Bay is presented.The interannual variability and trends in SIAF via the three passages are outlined.The connection to several large-scale atmospheric circulation modes is assessed.Over the period of 1988-2015,the average annual(October to the following September)SIAF amounts to 555×10^(3) km^(2),642×10^(3) km^(2),and 551×10^(3) km^(2) through Passages A,B,and C,respectively.These quantities are less than that observed through the Fram Strait(FS,707×10^(3) km^(2))of the corresponding period.The positive trends in annual SIAF,on the order of 53.1×10^(3) km^(2)/(10 a)and 43.2×10^(3) km^(2)/(10 a)(significant at the 95%confidence level),are identified at Passages A and B,respectively.The trend of the south passage(C),however,is slightly negative(-13.3×10^(3) km^(2)/(10 a),not statistically significant).The positive trends in annual SIAF through the Passages A and B are primarily attributable to the significant increases after 2000.The connection between the Baffin Bay sea ice export and the North Atlantic Oscillation is not significant over the studied period.By contrast,the association with the cross-gate sea level pressure difference is robust in the Baffin Bay(R equals 0.69 to 0.71,depending on the passages considered),but relatively weaker than that over FS(R=0.74).