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Pain perception enhancement in consecutive secondeye phacoemulsification cataract surgeries under topical anesthesia
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作者 jia-Wei Luo Yan-Hua Chen +3 位作者 jian-Feng Yu Yi-Xun Chen min ji Huai-jin Guan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第8期1510-1518,共9页
Cataract is the main cause of visual impairment and blindness worldwide while the only effective cure for cataract is still surgery.Consecutive phacoemulsification under topical anesthesia has been the routine procedu... Cataract is the main cause of visual impairment and blindness worldwide while the only effective cure for cataract is still surgery.Consecutive phacoemulsification under topical anesthesia has been the routine procedure for cataract surgery.However,patients often grumbled that they felt more painful during the second-eye surgery compared to the first-eye surgery.The intraoperative pain experience has negative influence on satisfaction and willingness for second-eye cataract surgery of patients with bilateral cataracts.Intraoperative ocular pain is a complicated process induced by the nociceptors activation in the peripheral nervous system.Immunological,neuropsychological,and pharmacological factors work together in the enhancement of intraoperative pain.Accumulating published literatures have focused on the pain enhancement during the secondeye phacoemulsification surgeries.In this review,we searched PubMed database for articles associated with pain perception differences between consecutive cataract surgeries published up to Feb.1,2024.We summarized the recent research progress in mechanisms and interventions for pain perception enhancement in consecutive secondeye phacoemulsification cataract surgeries.This review aimed to provide novel insights into strategies for improving patients’intraoperative experience in second-eye cataract surgeries. 展开更多
关键词 ocular pain cataract surgery topical anesthesia intraoperative experience second-eye phacoemulsification
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Five-year analysis of isolated pathogens and antibiotic resistance of ocular infections from two large tertiary comprehensive hospitals in east China
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作者 Pan-Pan Li Li Li +4 位作者 Jun-Fang Zhang Bai Qin Li-Hua Kang min ji Huai-jin Guan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第9期1707-1716,共10页
AIM:To analyze the spectrum of isolated pathogens and antibiotic resistance for ocular infections within 5y at two tertiary hospitals in east China.METHODS:Ocular specimen data were collected from January 2019 to Octo... AIM:To analyze the spectrum of isolated pathogens and antibiotic resistance for ocular infections within 5y at two tertiary hospitals in east China.METHODS:Ocular specimen data were collected from January 2019 to October 2023.The pathogen spectrum and positive culture rate for different infection location,such as keratitis,endophthalmitis,and periocular infections,along with antibiotic resistance were analyzed.RESULTS:We included 2727 specimens,including 827(30.33%)positive cultures.A total of 871 strains were isolated,530(60.85%)bacterial and 341(39.15%)fungal strains were isolated.Gram-positive cocci(GPC)were the most common ocular pathogens.The most common bacterial isolates were Staphylococcus epidermidis(25.03%),Staphylococcus aureus(7.46%),Streptococcus pneumoniae(4.59%),Corynebacterium macginleyi(3.44%),and Pseudomonas aeruginosa(3.33%).The most common fungal genera were Fusarium spp.(12.74%),Aspergillus spp.(6.54%),and Scedosporium spp.(5.74%).Staphylococcus epidermidis strains showed more than 50%resistance to fluoroquinolones.Streptococcus pneumoniae and Corynebacterium macginleyi showed more than 90%resistance to erythromycin.The percentage of bacteria showing multidrug resistance(MDR)significantly decreased(χ^(2)=17.44,P=0.002).CONCLUSION:GPC are the most common ocular pathogens.Corynebacterium macginleyi,as the fourth common bacterium,may currently be the local microbiological feature of east China.Fusarium spp.is the most common fungus.More than 50%of the GPC are resistant to fluoroquinolones,penicillins,and macrolides.However,the proportion of MDR strains has been reduced over time. 展开更多
关键词 ocular infections BACTERIA FUNGUS antibiotic resistance multidrug resistance
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Inflammation-related markers and prognosis of alpha-fetoprotein producing gastric cancer
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作者 Lu Zhang Yan-Ping Chen +4 位作者 min ji Le-Qian Ying Chun-Chun Huang jing-Yi Zhou Lin Liu 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第9期3875-3886,共12页
BACKGROUND Inflammation-related markers including neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),systemic immune-inflammation index(SII),systemic inflammation r... BACKGROUND Inflammation-related markers including neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),monocyte-to-lymphocyte ratio(MLR),systemic immune-inflammation index(SII),systemic inflammation response index(SIRI)and prognostic nutritional index(PNI)could reflect tumor immune microenvironment and predict prognosis of cancers.However,it had not been explored in alpha-fetoprotein(AFP)producing gastric cancer(GC).AIM To determine the predictive value of inflammation-related peripheral blood markers including as NLR,PLR,MLR,SII,SIRI and PNI in the prognosis of AFPproducing GC(AFPGC).Besides,this study would also compare the differences in tumor immune microenvironment,clinical characteristics and prognosis between AFPGC and AFP-GC patients to improve the understanding of this disease.METHODS 573 patients enrolled were retrospectively studied.They were divided into AFP+group(AFP≥20 ng/mL)and AFP-group(AFP<20 ng/mL),comparing the levels of NLR/PLR/MLR/SII/SIRI/PNI and prognosis.In AFP+group,the impact of NLR/PLR/MLR/SII/SIRI/PNI and their dynamic changes on prognosis were further explored.RESULTS Compared with AFP-patients,AFP+patients had higher NLR/PLR/MLR/SII/-SIRI and lower PNI levels and poorer overall survival(OS).In the AFP+group,mortality was significantly lower in the lower NLR/PLR/MLR/SII/SIRI group and higher PNI group.Moreover,the dynamic increase(NLR/PLR/MLR/SII/-SIRI)or decrease(PNI)was associated with the rise of mortality within 1 year of follow-up.CONCLUSION Compared with AFP-patients,the level of inflammation-related peripheral blood markers significantly increased in AFP+patients,which was correlated with OS of AFP+patients.Also,the gradual increase of SII and SIRI was associated with the risk of death within one year in AFP+patients.AFPGC should be considered as a separate type and distinguished from AFP-GC because of the difference in tumor immune microenvironment.It requires basic experiments and large clinical samples in the future. 展开更多
关键词 Alpha-fetoprotein producing gastric cancer Inflammatory indicator PROGNOSIS Dynamic changes Tumor immune microenvironment
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国内外代表性物理化学教材知识体系与教学内容的比较 被引量:3
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作者 张树永 侯文华 +8 位作者 刘俊吉 王新平 万坚 原弘 孙宏伟 姚加 王志勇 纪敏 郭玉鹏 《大学化学》 CAS 2023年第6期115-123,共9页
高质量的教材是支撑高水平教学的重要基础。本文对国内外15本具有代表性的物理化学教材进行了对比研究,对编写理念、教学内容、编排体系、教学目标以及教材的编写和出版情况等16个方面进行了对比研究,分析了我国物理化学教材的优势与不... 高质量的教材是支撑高水平教学的重要基础。本文对国内外15本具有代表性的物理化学教材进行了对比研究,对编写理念、教学内容、编排体系、教学目标以及教材的编写和出版情况等16个方面进行了对比研究,分析了我国物理化学教材的优势与不足,提出了十四五期间开展物理化学教材建设需要重视的7个方面建议,对教材的编写和出版具有一定的参考价值。 展开更多
关键词 物理化学 教材建设 对比研究 建设建议
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A bifunctional and recyclable catalyst:Amine and ionic liquid grafting on MOFs for the one-pot synthesis of N-aryl oxazolidin-2-ones 被引量:3
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作者 Siying Chong Tongtong Wang +4 位作者 Haijun Zhong Lingfei Xu Hailong Xu Zhongwu Lv min ji 《Green Energy & Environment》 SCIE CSCD 2020年第2期154-165,共12页
A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/IL–NH2-MIL-101.The structure and physical-chemical characterization of DMEDA/IL–NH2-MIL-101 and its precursors were characterized by S... A bifunctional heterogeneous catalyst was designed and synthesized,denoted DMEDA/IL–NH2-MIL-101.The structure and physical-chemical characterization of DMEDA/IL–NH2-MIL-101 and its precursors were characterized by SEM,N2 adsorption-desorption,XPS,FT-IR,PXRD,elemental analysis,and TGA techniques.The date showed that the two catalytic components of N,N-dimethylethylenediamine(DMEDA)and 1-butyl-3-methylimidazolium bromide(BmimBr)were chemically immobilized in NH2-MIL-101 nanocages.The amine of DMEDA was grafted onto carrier NH2-MIL-101 by N–Cr coordinate covalent bonds and the ionic liquid of BmimBr(IL(Br-))was anchored in the NH2-MIL-101 nanocages by'ship-in-a-bottle'method,in which the amidogen of NH2-MIL-101 condensed with N,N-carbonyldiimidazole(CDI)firstly,and then alkylated with 1-bromo butane.This novel heterogeneous catalyst with two different active sites can efficiently catalyze the synthesis of N-aryl oxazolidin-2-ones from carbon dioxide(CO2),epoxides,and anilines in one-pot under mild solvent-free conditions.It not only showed good stability and recoverability after five cycles but also exhibited shape selectivity for the substrate due to the synergic catalysis of amine,ionic liquid,and NH2-MIL-101.This novel bifunctional material is a promising solid catalyst for the green synthesis of N-aryl oxazolidin-2-ones. 展开更多
关键词 Metal-organic frameworks Ionic liquid immobilization N-aryl oxazolidin-2-ones One-pot synthesis Bifunctional heterogeneous catalyst
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iTrace和Pentacam及LOCSⅢ参数在晶状体混浊程度评估中的相关性
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作者 罗家伟 季敏 +2 位作者 秦苗苗 李鹏飞 管怀进 《国际眼科杂志》 CAS 北大核心 2023年第1期147-152,共6页
目的:分析iTrace视功能分析仪和Pentacam三维眼前节分析系统及晶状体混浊分级系统Ⅲ(LOCSⅢ)在年龄相关性白内障(ARC)患者晶状体混浊评估中的相关性。方法:前瞻性横断面研究。纳入2021-05/08本院眼科的ARC患者104例104眼。裂隙灯行LOCS... 目的:分析iTrace视功能分析仪和Pentacam三维眼前节分析系统及晶状体混浊分级系统Ⅲ(LOCSⅢ)在年龄相关性白内障(ARC)患者晶状体混浊评估中的相关性。方法:前瞻性横断面研究。纳入2021-05/08本院眼科的ARC患者104例104眼。裂隙灯行LOCSⅢ分级;iTrace获取晶状体混浊地形图分级(OMG)和晶状体功能失调指数(DLI);Pentacam获取核分级(PNS)和Scheimpflug图像,ImageJ测量Scheimpflug图像各区域的累积光密度(IntDen)。SPSS 26.0分析参数正态性和相关性。结果:各参数除3mm范围IntDen和6mm范围后囊区IntDen外均正态分布。PNS与NC、NO均正相关(r=0.521、0.440,均P<0.01)。3mm范围IntDen与NC、NO均正相关(rs=0.459、0.450,均P<0.01)。3mm范围核区IntDen与NC、NO均正相关(r=0.539、0.543,均P<0.01)。3mm范围后囊区IntDen与NC、NO均负相关(r=-0.315、-0.321,均P<0.01)。6mm范围IntDen与NC、NO均正相关(r=0.321、0.288,均P<0.01)。3mm范围DLI与NC、NO、PSC均负相关(r=-0.257、-0.234、-0.282,均P<0.01)。6mm DLI与NC、NO、PSC均负相关(r=-0.247、-0.304、-0.227,均P<0.05)。3mm范围OMG与CC、PSC均正相关(r=0.268、0.333,均P<0.01)。6mm范围OMG与CC、PSC均正相关(r=0.275、0.245,均P<0.05)。3mm范围DLI与PNS、3mm范围核区IntDen均负相关(r=-0.217、-0.197,均P<0.05)。结论:三种晶状体混浊评价系统各有优势,将各参数结合可更客观地评价晶状体各区混浊,为临床提供参考。 展开更多
关键词 Pentacam三维眼前节分析系统 晶状体混浊分级系统Ⅲ(LOCSⅢ) iTrace视功能分析仪 年龄相关性白内障
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Research on the Dynamic Response of Submerged Floating Tunnels to Wave Currents and Traffic Load 被引量:1
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作者 Bolin jiang Shanshan Wu +1 位作者 min ji Bo Liang 《Fluid Dynamics & Materials Processing》 EI 2023年第1期159-173,共15页
Submerged floating tunnel(SFTs)are typically subjected to complex external environmental and internal loads such as wave currents and traffic load.In this study,this problem is investigated through a finite element me... Submerged floating tunnel(SFTs)are typically subjected to complex external environmental and internal loads such as wave currents and traffic load.In this study,this problem is investigated through a finite element method able to account for fluid-structure interaction.The obtained results show that increasing the number of vehicles per unit length enhances the transverse vibrational displacements of the SFT cross sections.Under ultimate traffic load condition,one-way and two-way syntropic distributions can promote the dynamic responses of SFTs whereas two-way reverse distributions have the opposite effect. 展开更多
关键词 Submerged floating tunnel vehicle load dynamic response wave and current loads fluid-structure interaction
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Comparison of three fundus inspection methods during phacoemulsification in diabetic white cataract
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作者 Shu Su jian Wu +3 位作者 min ji Yu Guan Yao Shen Huai-jin Guan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第11期1782-1788,共7页
AIM:To investigate whether Wild Field Imaging System(WFIS SW-8000),25G endoilluminator,and intraoperative optical coherence tomography(iOCT)can perform realtime screening and diagnosing in patients with suspicious dia... AIM:To investigate whether Wild Field Imaging System(WFIS SW-8000),25G endoilluminator,and intraoperative optical coherence tomography(iOCT)can perform realtime screening and diagnosing in patients with suspicious diabetic retinopathy(DR)during phacoemulsification,especially in cases of white cataract.METHODS:A cross-sectional study was carried out.A total of 204 dense diabetic cataractous eyes of 204 patients with suspected DR treated from April 2020 to March 2021 were included.Phacoemulsification combined with intraocular lens implantation was performed.Following the removal of the lens opacity,the 25G endoilluminator,fundus photography,and iOCT were performed successively.Optical coherence tomography(OCT)and/or fundus fluorescein angiography(FFA)were used to verify the fundus findings postoperatively.Intraoperative and postoperative results were compared to verify the accuracy of intraoperative diagnosis in each group.RESULTS:Intraoperative and postoperative examinations revealed 58 and 62 eyes with DR,respectively(positive rate,28.43%and 30.39%,respectively).During the phacoemulsification,WFIS SW-8000 detected 44 eyes with DR(the detection rate,70.97%);25G endo-illuminator found 56 eyes with DR(the detection rate,90.32%);iOCT found 46 eyes with DR(the detection rate,74.19%);and 58 eyes with DR were found by combining the three methods(the detection rate,93.55%).There were statistically significant differences in the diagnostic sensitivity for DR among the methods(χ^(2)=16.36,P=0.001).CONCLUSION:WFIS SW-8000,25G endo-illuminator,iOCT,and especially their combination can be used to inspect the fundus and detect DR intraoperatively;they are helpful for the timely diagnosis and treatment of DR in patients with dense cataract. 展开更多
关键词 diabetic white cataract fundus inspection PHACOEMULSIFICATION diabetic retinopathy
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Metachronous primary esophageal squamous cell carcinoma and duodenal adenocarcinoma:A case report and review of literature
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作者 Chun-Chun Huang Le-Qian Ying +3 位作者 Yan-Ping Chen min ji Lu Zhang Lin Liu 《World Journal of Gastrointestinal Surgery》 SCIE 2023年第11期2627-2638,共12页
BACKGROUND The prevalence of multiple primary malignant neoplasms(MPMNs)is increasing in parallel with the incidence of malignancies,the continual improvement of diagnostic models,and the extended life of patients wit... BACKGROUND The prevalence of multiple primary malignant neoplasms(MPMNs)is increasing in parallel with the incidence of malignancies,the continual improvement of diagnostic models,and the extended life of patients with tumors,especially those of the digestive system.However,the co-existence of MPMNs and duodenal adenocarcinoma(DA)is rarely reported.In addition,there is a lack of compre-hensive analysis of MPMNs regarding multi-omics and the tumor microenvir-onment(TME).CASE SUMMARY In this article,we report the case of a 56-year-old man who presented with a complaint of chest discomfort and abdominal distension.The patient was diagnosed with metachronous esophageal squamous cell carcinoma and DA in the Department of Oncology.He underwent radical resection and chemotherapy for the esophageal tumor,as well as chemotherapy combined with a programmed death-1 inhibitor for the duodenal tumor.The overall survival was 16.6 mo.Extensive evaluation of the multi-omics and microenvironment features of primary and metastatic tumors was conducted to:(1)Identify the reasons responsible for the poor prognosis and treatment resistance in this case;and(2)Offer novel diagnostic and therapeutic approaches for MPMNs.This case demonstrated that the development of a second malignancy may be independent of the location of the first tumor.Thus,tumor recurrence(including metastases)should be distinguished from the second primary for an accurate diagnosis of MPMNs.CONCLUSION Multi-omics characteristics and the TME may facilitate treatment selection,improve efficacy,and assist in the prediction of prognosis.Core Tip:Multiple primary malignant neoplasms(MPMNs)are increasingly prevalent in clinical practice,most frequently in the digestive system.We report a rare case of MPMN with a combination of esophageal squamous cell carcinoma and duodenal adenocarcinoma.According to PubMed-indexed literature,there are no standard guidelines or expert consensus on the etiology and comprehensive treatment.We also conducted a detailed study of the features of primary and metastatic tumors.The aim of this report was to identify the reasons responsible for the poor prognosis and treatment resistance in this case through histological data and provide new diagnostic and treatment directions for MPMNs.INTRODUCTION Multiple primary malignant neoplasms(MPMNs),also termed multiple primary cancers,refer to two or more primary tumors that occur simultaneously or sequentially in a single or multiple organs[1].According to the time interval from the diagnosis of the first tumor,MPMNs are divided into synchronous cancer(SC)(<6 mo)and metachronous cancer(MC)(≥6 mo)[2].The detection rate of the second or multiple primary tumors is also on the rise due to newer diagnostic methods and treatments,as well as the longer survival times of patients with cancer.MPMNs are most commonly reported in the digestive system;however,their occurrence in combination with duodenal adenocarcinoma(DA)is extremely rare.In this article,we describe the case of a patient who had metachronous esophageal squamous cell carcinoma(ESCC)and DA with multiple metastases.In this analysis,we thoroughly examined the multi-omics features and tumor-related immune microenvironment.OUTCOME AND FOLLOW-UP The patient was eventually followed up until clinical death on June 18,2022(Figure 2),with an overall survival 16.6 mo. 展开更多
关键词 Multiple primary malignancies Esophageal tumor Duodenal adenocarcinoma Multi-omics Tumor microenvironment Case report
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AJUBA调控MST1 YAP1和TAZ因子促进食管鳞状细胞癌细胞体外增殖迁移和侵袭 被引量:5
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作者 苏丽萍 季敏 +5 位作者 路子扬 刘丽 王维娜 苗娜 张巍 蒲红伟 《中国肿瘤临床》 CAS CSCD 北大核心 2022年第7期331-337,共7页
目的:探讨AJUBA在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞中表达以及是否通过调控MST1、YAP1和TAZ因子影响细胞增殖、侵袭和迁移。方法:采用Western blot法检测AJUBA蛋白在食管癌细胞系KYSE30、KYSE150与KYSE450... 目的:探讨AJUBA在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)细胞中表达以及是否通过调控MST1、YAP1和TAZ因子影响细胞增殖、侵袭和迁移。方法:采用Western blot法检测AJUBA蛋白在食管癌细胞系KYSE30、KYSE150与KYSE450中的表达水平,构建shRNA干扰载体AJUBA转染至KYSE150食管癌细胞系;通过体外克隆形成实验、流式细胞术、划痕实验和Transwell实验探究AJUBA对KYSE150细胞的增殖、周期、迁移和侵袭能力的影响。采用RT-PCR和Western blot检测MST1、YAP1和TAZ mRNA和蛋白的表达;核质分离实验检测AJUBA、YAP1和TAZ蛋白表达情况。结果:稳定干扰AJUBA基因后,平板克隆实验结果显示,与阴性对照组相比,shAJUBA组的细胞克隆形成数量明显减少,差异具有统计学意义(P<0.001);流式细胞周期实验结果显示,shAJUBA组中的细胞阻滞于G0/G1期,G2/M期与S期细胞显著减少(P<0.05);划痕实验和Transwell侵袭实验结果显示,与AJUBA空染组相比,shAJUBA组细胞迁移能力和侵袭能力均明显减弱(P<0.001);RT-PCR与Western blot实验结果显示,shAJUBA组细胞中的MST1、YAP1、TAZmRNA与蛋白表达水平均显著降低,差异具有统计学意义(P<0.05)。核质分离实验结果提示AJUBA蛋白在正常食管鳞状上皮细胞株(SHEE)细胞核中表达,而在KYSE150细胞胞质与胞核中表达;YAP1和TAZ蛋白在SHEE细胞中不表达,在KYSE150细胞质与细胞核中表达,上述结果差异均具有统计学意义(均P<0.05)。结论:AJUBA促进食管癌细胞的增殖、侵袭与迁移,可能与激活MST1、YAP1、TAZ因子的表达而影响食管鳞状细胞癌进展有关。 展开更多
关键词 食管鳞状细胞癌 AJUBA蛋白 Hippo信号通路 增殖 侵袭
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静脉置管模式与HM患者CRBSI的病原菌分布及耐药性评估的相关性研究 被引量:8
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作者 戢敏 陈星 +5 位作者 刘娇 王川林 敬雪明 梅怡晗 李芸 梅小平 《中国现代医学杂志》 CAS 2018年第18期67-71,共5页
目的探讨静脉置管模式与恶性血液病(HM)患者导管相关性血流感染(CRBSI)及耐药性评估的相关性研究。方法回顾性分析1 335例在四家三级甲等医院血液肿瘤科住院的HM患者行静脉置管后发生CRBSI的病例资料。结果股静脉置管模式的CRBSI感染率... 目的探讨静脉置管模式与恶性血液病(HM)患者导管相关性血流感染(CRBSI)及耐药性评估的相关性研究。方法回顾性分析1 335例在四家三级甲等医院血液肿瘤科住院的HM患者行静脉置管后发生CRBSI的病例资料。结果股静脉置管模式的CRBSI感染率与锁骨下静脉、颈内静脉置管模式的CRBSI感染率比较差异有统计学意义(P<0.05)。CRBSI发生率高低与HM患者年龄、静脉穿刺次数、导管留置时间、规范换药与否、白细胞水平、是否合并糖尿病和激素及免疫抑制剂的使用与否相关(P<0.05)。头孢哌酮/舒巴坦、阿米卡星及亚胺培南西司他丁对革兰阴性菌耐药率<20.00%;鲍曼不动杆菌耐药率>50.00%;铜绿假单胞菌、肺炎克雷伯菌及大肠埃希菌耐药率均<50.00%。万古霉素、利奈唑胺及替加环素对3种革兰阳性菌不耐药;对利福平的耐药率<20.00%;对青霉素、苯唑西林、头孢哌酮/舒巴坦的抗菌药物敏感试验耐药率>50.00%。结论静脉置管模式与CRBSI有关,CRBSI病原菌以革兰阴性菌分布最多,对常用抗菌药物的耐药率较高。 展开更多
关键词 恶性血液病 静脉置管模式 导管相关性血流感染 病原菌 耐药性
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手术联合自拟益气健脾润肠通便方治疗女性直肠前突所致出口梗阻型便秘的效果观察 被引量:9
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作者 戢敏 郭光丽 +4 位作者 王芳 张迪 刘凤玲 魏少兴 杨冬 《中国现代医学杂志》 CAS 北大核心 2021年第14期7-12,共6页
目的探讨手术联合自拟益气健脾润肠通便方治疗女性直肠前突所致出口梗阻型便秘的疗效及安全性。方法选取2017年6月—2020年5月在石家庄市中医院行手术治疗的80例女性直肠前突致出口梗阻型便秘患者为研究对象。采用随机数字表法将其分为... 目的探讨手术联合自拟益气健脾润肠通便方治疗女性直肠前突所致出口梗阻型便秘的疗效及安全性。方法选取2017年6月—2020年5月在石家庄市中医院行手术治疗的80例女性直肠前突致出口梗阻型便秘患者为研究对象。采用随机数字表法将其分为对照组和观察组,每组40例。两组均行经阴道修补术、痔上黏膜环形错位套扎吻合术(EPH),观察组在此基础上于术后2 d开始口服自拟益气健脾润肠通便中药方,14 d为1个疗程,连续服用2个疗程后评估疗效,记录不良反应发生情况,比较远期疗效。结果观察组治疗前后排便频率、排便困难程度、粪便性状、排便时间、排便不尽感、头昏、乏力、口干口渴评分的差值大于对照组(P<0.05)。观察组患者临床有效率高于对照组(95.00%VS 80.00%,P<0.05)。两组患者的腹胀/腹泻、恶心/呕吐、出血、疼痛、尿潴留发生率及不良反应总发生率比较,差异无统计学意义(P>0.05),且上述不良反应均为轻度,无重度不良反应。观察组治疗前后出口梗阻型便秘评分(ODS)的差值大于对照组(P<0.05),复发率低于对照组(P<0.05)。结论经阴道修补术、EPH术后口服自拟益气健脾润肠通便中药方,可有效改善女性直肠前突所致出口梗阻型便秘临床症状,提高近、远期疗效,降低复发率,且不增加不良反应发生风险。 展开更多
关键词 益气健脾润肠通便方 经阴道修补术 痔上黏膜环形错位套扎吻合术 女性 直肠前突 出口梗阻型便秘
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手足口病患儿细胞免疫功能与相关细胞因子水平变化的临床研究 被引量:13
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作者 陈星 戢敏 +3 位作者 喻雪琴 陈芳 梅怡晗 梅小平 《中国现代医学杂志》 CAS 2019年第22期58-62,共5页
目的分析手足口病(HFMD)患儿循环血T淋巴细胞亚群、NK细胞及细胞因子水平变化及意义。方法回顾性分析2016年1月—2018年6月在川北医学院附属医院就诊的68例HFMD患儿,分析其治疗前后细胞免疫功能与相关细胞因子水平变化,探讨其与病情程... 目的分析手足口病(HFMD)患儿循环血T淋巴细胞亚群、NK细胞及细胞因子水平变化及意义。方法回顾性分析2016年1月—2018年6月在川北医学院附属医院就诊的68例HFMD患儿,分析其治疗前后细胞免疫功能与相关细胞因子水平变化,探讨其与病情程度的关系。结果68例HFMD患儿作为观察组,该院健康体检儿童25例作为对照组。观察组以单一病毒感染最为常见,肠道病毒71(EV-71)构成比最高(47.06%),其次为EV-71+CA16的(14.71%)。观察组治疗前CD4+T、CD8+T、NK细胞水平低于对照组(P<0.05);观察组白细胞介素-10(IL-10)、白细胞介素-17(IL-17)及肿瘤坏死因子-α(TNF-α)水平高于对照组(P<0.05)。重型HFMD组治疗前CD4+T、CD8+T水平低于普通型HFMD组(P<0.05),而NK细胞水平高于普通型HFMD组(P<0.05);重型HFMD组IL-10、IL-17及TNF-α高于普通型HFMD组(P<0.05)。观察组患儿治疗7 d后CD4+T、CD8+T及NK细胞水平较治疗前高(P<0.05),而治疗后IL-10、IL-17及TNF-α水平较治疗前低(P<0.05)。结论HFMD发生机制与机体细胞免疫功能异常和相关细胞因子水平失衡相关,并对疾病的发生、发展、病情评估及转归有重要指导作用。 展开更多
关键词 手足口病 T淋巴细胞亚群 白细胞介素10 白细胞介素17 肿瘤坏死因子α
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循环血TLR-4、TGF-β1表达水平与HBV相关性肝硬化患者病情程度的相关性分析 被引量:16
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作者 陈芳 喻雪琴 +3 位作者 戢敏 陈星 梅怡晗 梅小平 《中国现代医学杂志》 CAS 2019年第21期71-75,共5页
目的检测Toll样受体4(TLR-4)、转化生长因子β1(TGF-β1)在乙型肝炎病毒(HBV)相关性肝硬化患者中的表达水平,探讨其在HBV相关性肝硬化发生、发展中的作用。方法选取2016年1月2017年12月川北医学院附属医院就诊的HBV携带者25例(HBV携带... 目的检测Toll样受体4(TLR-4)、转化生长因子β1(TGF-β1)在乙型肝炎病毒(HBV)相关性肝硬化患者中的表达水平,探讨其在HBV相关性肝硬化发生、发展中的作用。方法选取2016年1月2017年12月川北医学院附属医院就诊的HBV携带者25例(HBV携带组),慢性乙型肝炎(CHB)患者50例(CHB组),HBV相关性肝硬化患者139例(HBV相关性肝硬化组),选取同期25例健康体检者为对照(健康对照组)。检测TLR-4、TGF-β1及HBV DNA在各研究对象循环血中的表达水平并检测肝功能指标。比较TLR-4、TGF-β1表达水平差异及与丙氨酸转氨酶(ALT)、HBV载量的相关性。结果HBV携带组循环血TLR-4、TGF-β1水平与健康对照组比较差异无统计学意义(P>0.05)。HBV相关性肝硬化组TLR-4、TGF-β1表达水平较健康对照组、HBV病毒携带组、CHB组水平升高(P<0.05),HBV相关性肝硬化组TLR-4、TGF-β1表达水平较CHB组升高(P<0.05);在HBV相关性肝硬化患者中,随着肝损或肝纤维化程度加重,循环血TLR-4、TGF-β1表达水平升高,Child-Pugh C级HBV相关性肝硬化患者循环血TLR-4、TGF-β1水平最高,Child-Pugh A、B、C级3者间比较,差异有统计学意义(P<0.05);HBV相关性肝硬化患者循环血TLR-4、TGF-β1水平与HBV DNA载量呈负相关(r=-0.497和-0.581,均P=0.000);与ALT水平呈正相关(r=0.572和0.619,均P=0.000);HBV相关性肝硬化患者循环血TGF-β1与TLR-4表达水平呈正相关(r=0.736,P=0.000)。结论HBV相关性肝硬化患者循环血TLR-4、TGF-β1高表达,可能诱导参与肝纤维化的发生,是反映HBV相关性肝硬化病情与纤维化程度的重要指标之一。 展开更多
关键词 肝硬化 肝炎病毒 乙型 TOLL样受体4 转化生长因子Β1
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“双一流”建设背景下物理化学课程教学改革与实践 被引量:25
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作者 纪敏 王新葵 +2 位作者 孙延波 王旭珍 王新平 《大学化学》 CAS 2021年第1期11-16,共6页
在“双一流”建设的背景下,物理化学教学秉承“以学生为中心,以目标为导向”的教学理念,通过问题引导的启发式教学模式,从科学发展的历史角度出发,阐述相关概念、定律,并将学科前沿和应用案例引入课堂,培养学生的创新意识和科学思维方... 在“双一流”建设的背景下,物理化学教学秉承“以学生为中心,以目标为导向”的教学理念,通过问题引导的启发式教学模式,从科学发展的历史角度出发,阐述相关概念、定律,并将学科前沿和应用案例引入课堂,培养学生的创新意识和科学思维方法。通过翻转课堂和分组讨论的方式,培养学生的自学能力和团队协作精神。 展开更多
关键词 双一流 物理化学 课程改革
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创一流:应用化学专业建设的改革与实践 被引量:9
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作者 纪敏 孟长功 +2 位作者 姜文凤 吴硕 赵艳秋 《大学化学》 CAS 2021年第5期118-122,共5页
大连理工大学应用化学专业经历30多年的发展与建设,已经形成了鲜明的专业特色和优势。本文全面介绍了专业在人才培养模式、改革课程教学体系、提升专业教学条件、加强师资队伍建设、完善教育管理机制等方面的举措和实施成效。
关键词 专业建设 应用化学 改革与实践
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HBV相关性肝病患者外周血PBMCs表面Tim-3、PD-1表达水平检测及意义 被引量:10
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作者 喻雪琴 张诗琬 +4 位作者 陈芳 陈星 戢敏 梅怡晗 梅小平 《中国现代医学杂志》 CAS 2019年第19期43-47,共5页
目的分析乙型肝炎病毒(HBV)感染后相关肝病患者外周血单个核细胞(PBMCs)表面T细胞免疫球蛋白黏蛋白分子-3(Tim-3)、程序性死亡受体-1(PD-1)表达水平变化及意义。方法选取2017年1~12月川北医学院附属医院HBV携带者20例,慢性乙型肝炎(CHB... 目的分析乙型肝炎病毒(HBV)感染后相关肝病患者外周血单个核细胞(PBMCs)表面T细胞免疫球蛋白黏蛋白分子-3(Tim-3)、程序性死亡受体-1(PD-1)表达水平变化及意义。方法选取2017年1~12月川北医学院附属医院HBV携带者20例,慢性乙型肝炎(CHB)患者30例,重型乙型肝炎患者20例,乙肝肝硬化患者30例,肝细胞癌患者20例,以同期20例健康体检者作为健康对照组。检测各研究对象外周血PBMCs表面Tim-3、PD-1表达水平。结果 PBMCs表面Tim-3、PD-1表达水平在健康对照组最低,与HBV携带组比较差异无统计学意义(P>0.05),随着病情加重,PBMCs表面Tim-3、PD-1表达水平逐渐升高,在重型乙型肝炎组、肝细胞癌组最高,各组患者PBMCs表面Tim-3、PD-1表达水平与健康对照组、HBV携带者组比较,差异有统计学意义(P<0.05);HBV感染患者PBMCs表面Tim-3、PD-1表达水平与HBV DNA载量呈负相关(r=-0.431和-0.422,均P<0.05),与ALT、AST水平呈正相关(r=0.214、0.325、0.234和0.354,均P <0.05);HBV感染患者总体PBMCs表面Tim-3的表达水平与PD-1表达量呈正相关(r=0.967,P <0.05)。结论免疫负性调节因子Tim-3、PD-1与HBV相关性肝病患者肝组织炎症及纤维化发生相关,对Tim-3、PD-1水平调节可能为其临床免疫治疗提供新思路。 展开更多
关键词 肝疾病 肝炎病毒 乙型 外周血单个核细胞 T细胞免疫球蛋白黏蛋白分子-3 程序性死亡受体-1
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Epstein-Barr病毒感染相关性肝损伤对循环血T淋巴细胞亚群影响的相关性分析 被引量:3
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作者 戢敏 陈星 +3 位作者 喻雪琴 陈芳 梅怡晗 梅小平 《中国现代医学杂志》 CAS 2020年第3期64-68,共5页
目的探讨Epstein-Barr病毒(EBV)感染相关性肝损伤对循环血T淋巴细胞亚群的影响。方法回顾性分析2012年6月-2018年8月川北医学院附属医院确诊为EBV感染致相关性肝损伤的56例患者的临床资料并对其进行相关性分析。结果56例EBV病毒感染者中... 目的探讨Epstein-Barr病毒(EBV)感染相关性肝损伤对循环血T淋巴细胞亚群的影响。方法回顾性分析2012年6月-2018年8月川北医学院附属医院确诊为EBV感染致相关性肝损伤的56例患者的临床资料并对其进行相关性分析。结果56例EBV病毒感染者中,单纯EBV病毒感染者36例(64.29%),EBV合并感染者20例(35.71%),其中合并甲型肝炎病毒(HAV)感染2例(3.57%),合并乙型肝炎病毒(HBV)感染8例(14.29%),合并丙型肝炎病毒(HCV)感染1例(1.79%),合并科萨奇B组病毒(CVB)感染2例(3.57%),合并带状疱疹病毒(VZV)感染3例(5.36%),合并HBV、VZV 4例(7.14%);单纯EBV病毒感染与EBV病毒合并感染组的肝损伤指标均较健康对照组升高(P<0.05),EBV合并感染组肝损伤程度较EBV单纯感染组重,两者间比较,差异有统计学意义(P<0.05);治疗前单纯EBV感染组,EBV合并感染组的CD3+T、CD8+T细胞亚群水平高于健康对照组,CD4+T、CD4+/CD8+T水平低于健康对照组(P<0.05),EBV合并感染组CD3+T、CD8+T高于EBV单纯感染组,CD3+T、CD4+/CD8+T水平低于EBV单纯感染组(P<0.05);治疗后CD3+T、CD8+T较治疗前下调,CD4+T、EBV合并感染组的CD4+/CD8+T上调(P<0.05),CD3+T、CD4+T、CD8+T水平高于EBV单纯感染组(P<0.05);单纯EBV感染与EBV合并感染组治疗前后CD3+T、CD4+T、CD8+T、CD4+/CD8+T水平与EBV-DNA载量无相关性(P>0.05)。结论EBV感染不仅可引起不同程度的肝功能损害,也会导致T淋巴细胞亚群功能紊乱,重视EBV病毒筛查有利于早诊断、早治疗。 展开更多
关键词 肝炎 EB病毒 EB病毒载量 T淋巴细胞亚群
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Genetic diagnosis strategy of hereditary non-polyposis colorectal cancer 被引量:4
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作者 jian-Qiu Sheng Hong Zhang +11 位作者 min ji Lei Fu Hong Mu ming-Zhi Zhang ji-Sheng Huang min Han Ai-Qin Li Zhi Wei Zi-Qin Sun Zi-Tao Wu Chang-Hong Xia Shi-Rong Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第8期983-989,共7页
AIM: To study the characteristics of mismatch repair gene mutation of Chinese hereditary non-polyposis colorectal cancer (HNPCC) and hMLH1 gene promoter methylation, and to improve the screening strategy and explore t... AIM: To study the characteristics of mismatch repair gene mutation of Chinese hereditary non-polyposis colorectal cancer (HNPCC) and hMLH1 gene promoter methylation, and to improve the screening strategy and explore the pertinent test methods. METHODS: A systematic analysis of 30 probands from HNPCC families in the north of China was performed by immunohistochemistry, microsatellite instability (MSI), gene mutation and methylation detection. RESULTS: High frequency microsatellite instability occurred in 25 probands (83.3%) of HNPCC family. Loss of hMLH1 and hMSH2 protein expression accounted for 88% of all microsatellite instability. Pathogenic muta-tion occurred in 14 samples and 3 novel mutational sites were discovered. Deletion of exons 1-6, 1-7 and 8 of hMSH2 was detected in 3 samples and no large fragment deletion was found in hMLH1. Of the 30 probands, hMLH1 gene promoter methylation occurred in 3 probands. The rate of gene micromutation detection combined with large fragment deletion detection was 46.7%-56.7%. The rate of the two methods in combination with methylation detection was 63.3%. CONCLUSION: Scientific and rational detection strategy can improve the detection rate of HNPCC. Based on traditional molecular genetics and combined with epigenetics, multiple detection methods can accurately diagnose HNPCC. 展开更多
关键词 Hereditary non-polyposis colorectal cancer Gene mutation Mismatch repair HMSH2 HMLH1 Large fragment deletion METHYLATION
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Impacts of Passive Smoking on Learning and Memory Ability of Mouse Offsprings and Intervention by Antioxidants 被引量:4
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作者 jiE YANG LI-NA jiANG +8 位作者 ZHEN-LI YUAN Yu-FEIZHENG Lu WANG min ji ZHI-QIANG SHEN XIN-WEI WANG QIANG MA ZHU-GE XI JUN-WEN LI 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2008年第2期144-149,共6页
Objective To determine the impact of passive smoking and the protective effect of antioxidants such as vitamin E and quercetin on learning and memory ability of mouse offsprings. Methods A passive smoking model of pre... Objective To determine the impact of passive smoking and the protective effect of antioxidants such as vitamin E and quercetin on learning and memory ability of mouse offsprings. Methods A passive smoking model of pregnant mice was established. Learning and memory ability was evaluated by the water maze test and long term potentiation (LTP). Nitric oxide (NO), content, nitric oxide synthase (NOS), acetylcholinesteras (Ache) activity in brain, vitamin E concentration, and reactive oxygen species (ROS) in serum were determined. The latency period (the time during which the mice swim from the starting position to the ending position) and errors (the number of mice entering the blind end) in control and antioxidant intervention groups were compared with those in the smoke exposure group after 6 days. Results The latency period as well as errors in the air, control diet, tobacco smoke (TS), and vitamin E diet groups were decreased significantly as compared with the TS and control diet groups (P〈O.05). LTP was restrained in the TS and control diet groups. LTP in all the antioxidant diet groups was significantly increased compared with the TS and control diet groups. In addition, NOS and acetylcholinesteras (Ache) activitiy was significantly higher in the TS and control diet groups than in the air and control diet group. NO content was not significantly different among the different groups, and significantly lower in the TS and vitamin E diet groups than in the TS group, control diet group, quercetin diet group, and mixture diet group (P〈0.05). Vitamin E concentration and ROS activity in serum were correlated with the outcome of water maze and LTP. Conclusion Passive smoking reduces LTP formation by disturbing the hippocampus function of mice, by decreasing NOS (especially vitamin E) partially improve the learning and memory smoke during pregnancy. and Ache activity and increasing NO content. Antioxidants ability of offsprings whose mothers are exposed to tobacco 展开更多
关键词 Passive smoking Mice offspring Learning and memory ability Long term potentiation Antioxidant intervention
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