Bread wheat(Triticum aestivum)is a staple food crop worldwide.The genetic dissection of important nutrient traits is essential for the biofortification of wheat to meet the nutritional needs of the world's growing...Bread wheat(Triticum aestivum)is a staple food crop worldwide.The genetic dissection of important nutrient traits is essential for the biofortification of wheat to meet the nutritional needs of the world's growing population.Here,45,298 single-nucleotide polymorphisms(SNPs)from 55K chip arrays were used to genotype a panel of 768 wheat cultivars,and a total of 154 quantitative trait loci(QTLs)were detected for eight traits under three environments by genome-wide association study(GWAS).Three QTLs(qMn-3B.1,qFe-3B.4,and qSe-3B.1/qFe-3B.6)detected repeatedly under different environments or traits were subjected to subsequent analyses based on linkage disequilibrium decay and the P-values of significant SNPs.Significant SNPs in the three QTL regions formed six haplotypes for qMn-3B.1,three haplotypes for qFe-3B.4,and three haplotypes for qSe-3B.1/qFe-3B.6.Phenotypic analysis revealed significant differences among haplotypes.These results indicated that the concentrations of several nutrient elements have been modified during the domestication of landraces to modern wheat.Based on the QTL regions,we identified 15 high-confidence genes,eight of which were stably expressed in different tissues and/or developmental stages.TraesCS3B02G046100 in qMn-3B.1 and TraesCS3B02G199500 in qSe-3B.1/qFe-3B.6 were both inferred to interact with metal ions according to the Gene Ontology(GO)analysis.TraesCS3B02G199000,which belongs to qSe-3B.1/qFe-3B.6,was determined to be a member of the WRKY gene family.Overall,this study provides several reliable QTLs that may significantly affect the concentrations of nutrient elements in wheat grain,and this information will facilitate the breeding of wheat cultivars with improved grain properties.展开更多
The Laji Shan—Jishi Shan tectonic belt(LJTB),located in the southern part of the northeastern Tibetan Plateau(NETP),is a tectonic window to reveal regional tectonic deformation in the NETP.However,its kinematics in t...The Laji Shan—Jishi Shan tectonic belt(LJTB),located in the southern part of the northeastern Tibetan Plateau(NETP),is a tectonic window to reveal regional tectonic deformation in the NETP.However,its kinematics in the Holocene remains controversial.We obtain the latest and dense horizontal velocity field based on data collected from our newly constructed and existing GNSS stations.Combined with fault kinematics from geologic observations,we analyze the crustal deformation characteristics along the LJTB.The results show that:(1)The Laji Shan fault(LJF)is inactive,and the northwest-oriented Jishi Shan fault(JSF)exhibits a significant dextral and thrust slip.(2)The transpression along the arc-shaped LJTB accommodates deformation transformation between the dextral Riyue Shan fault and the sinistral west Qinling fault.(3)With the continuous pushing of the Indian plate,internal strains in the Tibetan Plateau are continuously transferred in the northeast via the LJTB as they are gradually dissipated near the LJTB and translated into significant crustal uplift in these regions.展开更多
Transient deformation,such as post-seismic slip,slow slip and pre-seismic slip events,is a limited lowfrequency deformation that can last for hours to months,in contrast to a sudden slip on a fault caused by earthquak...Transient deformation,such as post-seismic slip,slow slip and pre-seismic slip events,is a limited lowfrequency deformation that can last for hours to months,in contrast to a sudden slip on a fault caused by earthquakes.Continuous Global Positioning System(CGPS),one of the most common geodetic techniques for continuously monitoring crustal deformation,is capable of capturing transient deformation signals.A critical point in characterizing transient deformation signals is the development of extracting and deciphering transient deformation signals from a huge and messy data set of position time series.Principal Component Analysis(PCA),one of the data-driven methods,has been employed to derive transient deformation signals from position time series combing with Kalman filtering.Independent Component Analysis(ICA)performs well in recovering and separating the sources of observed data,however,it is rarely used in extracting transient deformation signals.We aim to decompose the transient deformation signals from the daily GPS observation deployed in Akutan Island from 2007 to 2015 with the ICA method and obtain the spatiotemporal responses to the source signals of transient deformation.Our results indicate that ICA method can also characterize effectively transient deformation signals spatially and temporally.Additionally,the independent relationship between sources obtained by ICA allows for flexibility in linearly combining different sources.展开更多
This study analyzed the vertical deformation before and after the 2022 Menyuan Ms6.9 earthquake in Qinghai Province,China,using leveling profiles across faults measured from Minle County in Gansu Province to Menyuan C...This study analyzed the vertical deformation before and after the 2022 Menyuan Ms6.9 earthquake in Qinghai Province,China,using leveling profiles across faults measured from Minle County in Gansu Province to Menyuan County in Qinghai Province.Our results suggest the following:(1)The amplitude of regional vertical differential motion near the Sunna-Qilian and Lenglongling faults within the Qilian Shan increased before the 2022 Menyuan earthquake.It was accompanied by the emergence of high gradient deformation zones.Deformation at the Tongziba cross-fault leveling site near the Sunan-Qilian fault was considerable.In contrast,deformation at the Daliang cross-fault leveling site near the stepover region(adjacent to the epicenter)between the Lenglongling and Tuolaishan faults was minor.After 2018,vertical deformation at the Tongziba site notably accelerated,while that at the Daliang site was insignificant.(2)After the 2022 Menyuan earthquake,140—150 mm of subsidence deformation occurred near the Daliang site,while the Tongziba site did not experience significant deformation.(3)Vertical deformation before and after the 2022 Menyuan earthquake conforms with the elastic-rebound theory,and the evolution of pre-earthquake deformation was consistent with the strike-slip fault deformation pattern at different seismogenic stages,i.e.,the relative motion near the locked fault in the late seismogenic stage gradually weakened.The characteristics of strain accumulation and release derived from the vertical deformation before and after the Menyuan MS6.9 earthquake help understand the deformation process of earthquake preparation and earthquake precursors.展开更多
The Chinese wheat landrace Kaixianluohanmai(KL)expresses the ph-like phenotype.A major QTL,QPh.sicau-3A(syn.phKL),responsible for this effect has been mapped to chromosome arm 3AL.This study presents some characterist...The Chinese wheat landrace Kaixianluohanmai(KL)expresses the ph-like phenotype.A major QTL,QPh.sicau-3A(syn.phKL),responsible for this effect has been mapped to chromosome arm 3AL.This study presents some characteristics of homoeologous pairing and recombination induced by phKL.In KL haploids,the level of homoeologous pairing was elevated relative to Ph1 Chinese Spring(CS)haploids.There was a clear preference for A–D pairing and less frequent for A–B and B–D,reflecting the higher levels of affinity between genomes A and D in wheat.The characteristics of pairing were affected by temperature and magnesium ion supplementation.The suitability of phKL for chromosome engineering was tested on three pairs of homoeologues:2Sv-2B,2Sv-2D,and 2RL-2BL.The recombination rates were 1.68%,0.17%,and 0%,respectively.The phKL locus in KL induced a moderate level of homoeologous chromosome pairing and recombination when the Ph1 locus of wheat was present,both in wheat haploids and hexaploids.The Ph1-imposed criteria for chromosome pairing and crossing over were relaxed to some degree,permitting homoeologous crossing over but only between closely related chromosomes;there was no crossing over between more differentiated chromosomes.Therefore,the phKL system(QPh.sicau-3A)can be a useful tool in chromosome engineering of wheat to transfer genes from closely related species with the benefit of reduced genomic chaos generated by the ph1b mutation.展开更多
Astragaloside Ⅳ is the main active compound of Astragalus membranaceus. Astragaloside Ⅳ has strong anti-oxidative activities and protective effects against progression of peripheral neuropathy. In this study, we det...Astragaloside Ⅳ is the main active compound of Astragalus membranaceus. Astragaloside Ⅳ has strong anti-oxidative activities and protective effects against progression of peripheral neuropathy. In this study, we determined whether astragaloside Ⅳ protects retinal ganglion cells(RGC) from oxidative stress injury using the rat RGC-5 cell line. Hydrogen peroxide(H_2O_2) was used to induce oxidative stress injury, with the protective effect of astragaloside Ⅳ examined. Cell Counting Kit-8 and 4′,6-diamidino-2-phenylindole staining showed that astragaloside Ⅳ increased cell survival rate and decreased apoptotic cell number. Flow cytometry showed that astragaloside Ⅳ decreased H_2O_2-induced reactive oxygen species levels. While laser confocal microscopy showed that astragaloside Ⅳ inhibited the H_2O_2-induced decrease of mitochondrial membrane potential. Western blot assay showed that astragaloside Ⅳ reduced cytochrome c release induced by H_2O_2, inhibited Bax and caspase-3 expression, and increased Bcl-2 expression. Altogether, these results indicate that astragaloside Ⅳ has potential protective effects against H_2O_2-induced oxidative stress in retinal ganglion cells.展开更多
目的:探讨超声引导下腰丛-坐骨神经阻滞对老年股骨颈骨折患者疼痛、应激反应的影响。方法:回顾性分析96例老年股骨颈骨折患者临床资料,51例全麻患者作为对照组,45例超声引导下腰丛-坐骨神经阻滞联合全麻的患者作为观察组。比较两组麻醉...目的:探讨超声引导下腰丛-坐骨神经阻滞对老年股骨颈骨折患者疼痛、应激反应的影响。方法:回顾性分析96例老年股骨颈骨折患者临床资料,51例全麻患者作为对照组,45例超声引导下腰丛-坐骨神经阻滞联合全麻的患者作为观察组。比较两组麻醉情况、术后疼痛度、应激反应及并发症情况。结果:观察组术后苏醒时间、定向力恢复时间、麻醉起效时间、运动阻滞起效时间、感觉阻滞起效时间均短于对照组(P<0.05)。观察组术后各时间点VAS评分均低于对照组(P<0.05),且两组VAS评分在术后24 h明显降低(P<0.05)。与麻醉前相比,两组麻醉后15 min、术后15 min NE、ALD及PRA水平均升高(P<0.05);与麻醉后15 min相比,两组术后15 min NE、ALD及PRA水平均升高(P<0.05),且观察组上述各时间点NE、ALD及PRA水平均低于对照组(P<0.05)。观察组并发症发生率为8.89%,低于对照组的25.49%(P<0.05)。结论:超声引导下腰丛-坐骨神经阻滞在老年股骨颈骨折手术过程中麻醉效果较好,可提高患者手术镇痛效果,降低应激反应,且安全可靠。展开更多
Hainan Island is a seismic active region, where Qiongshan M7.5 earthquake occurred in 1605 and several seismic belts appeared in recent years, especially the NS trending seismic belt (NSB) located in the northeast p...Hainan Island is a seismic active region, where Qiongshan M7.5 earthquake occurred in 1605 and several seismic belts appeared in recent years, especially the NS trending seismic belt (NSB) located in the northeast part of the island. Here is also a magmatic active region. The lava from about 100 volcanoes covered more than 4000 km^2. The latest eruptions occurred on Ma'anling-Lei Huling volcanoes within 10,000 years. The neotectonic movement has been determined by geological method in the island and its adjacent areas. In the paper, the present-day 3D crustal movement is obtained by using Global Positioning System (GPS) data observed from 2009 to 2014 and leveling observations measured in 1970s and 1990s respectively. The results show the horizontal movement is mainly along SEE direction relative to the Eurasian Plate. The velocities are between 4.01 and 6.70 mm/a. The tension rate near the NSB is less than I mm/a. The vertical movement shows the island uplifts as a whole with respect to the reference benchmark Xiuyinggang. The average uplifting rate is 2.4 mm/a. The rates are 2-3 mm/a in the northwest and 3-5mm/a in the northwest. It shows the deformation pattern of the southwest island is upward relative to the northeast, which is different from the result inferred from the coastal change and GPS. Haikou and its adjacent region present a subsidence in a long time. The southern part of the middle segment of the Wangwu-Wenjiao fault uplifts relative to the northern. Meanwhile, the western part uplifts relative to the eastern NSB. The vertical crustal motion and the two normal faults nearly correspond to the terrain. The NSB is located along the Puqiangang-Dazhibo fault, which is assessed as a segmented fault with a dip of 80°-90° and party exposed. The 3D deformations and other studies reveal the present activities of earthquakes, volcanoes and the faults. The small earthquakes will still occur in the NS belt and the volcanoes are not active now.展开更多
基金This work was supported by grants from the Natural Science Foundation of Shandong Province,China(ZR2020MC096,ZR2021ZD31,and ZR2020MC151)the Agricultural Variety Improvement Project of Shandong Province,China(2021LZGC013 and 2022LZGC002).
文摘Bread wheat(Triticum aestivum)is a staple food crop worldwide.The genetic dissection of important nutrient traits is essential for the biofortification of wheat to meet the nutritional needs of the world's growing population.Here,45,298 single-nucleotide polymorphisms(SNPs)from 55K chip arrays were used to genotype a panel of 768 wheat cultivars,and a total of 154 quantitative trait loci(QTLs)were detected for eight traits under three environments by genome-wide association study(GWAS).Three QTLs(qMn-3B.1,qFe-3B.4,and qSe-3B.1/qFe-3B.6)detected repeatedly under different environments or traits were subjected to subsequent analyses based on linkage disequilibrium decay and the P-values of significant SNPs.Significant SNPs in the three QTL regions formed six haplotypes for qMn-3B.1,three haplotypes for qFe-3B.4,and three haplotypes for qSe-3B.1/qFe-3B.6.Phenotypic analysis revealed significant differences among haplotypes.These results indicated that the concentrations of several nutrient elements have been modified during the domestication of landraces to modern wheat.Based on the QTL regions,we identified 15 high-confidence genes,eight of which were stably expressed in different tissues and/or developmental stages.TraesCS3B02G046100 in qMn-3B.1 and TraesCS3B02G199500 in qSe-3B.1/qFe-3B.6 were both inferred to interact with metal ions according to the Gene Ontology(GO)analysis.TraesCS3B02G199000,which belongs to qSe-3B.1/qFe-3B.6,was determined to be a member of the WRKY gene family.Overall,this study provides several reliable QTLs that may significantly affect the concentrations of nutrient elements in wheat grain,and this information will facilitate the breeding of wheat cultivars with improved grain properties.
基金supported by the National Science Foundation of China(41874117)the Second Tibetan Plateau Scientific Expedition and Research Program(SETP)(2019QZKK0901)Natural Science Basic Research Program of Shaanxi(Program No.2023-JC-ON-0309)。
文摘The Laji Shan—Jishi Shan tectonic belt(LJTB),located in the southern part of the northeastern Tibetan Plateau(NETP),is a tectonic window to reveal regional tectonic deformation in the NETP.However,its kinematics in the Holocene remains controversial.We obtain the latest and dense horizontal velocity field based on data collected from our newly constructed and existing GNSS stations.Combined with fault kinematics from geologic observations,we analyze the crustal deformation characteristics along the LJTB.The results show that:(1)The Laji Shan fault(LJF)is inactive,and the northwest-oriented Jishi Shan fault(JSF)exhibits a significant dextral and thrust slip.(2)The transpression along the arc-shaped LJTB accommodates deformation transformation between the dextral Riyue Shan fault and the sinistral west Qinling fault.(3)With the continuous pushing of the Indian plate,internal strains in the Tibetan Plateau are continuously transferred in the northeast via the LJTB as they are gradually dissipated near the LJTB and translated into significant crustal uplift in these regions.
基金supported by the National Natural Science Foundation of China projects(U2139205,42072243)Natural Science Basic Research Program of Shaanxi(Program No.2023-JC-QN-0309)。
文摘Transient deformation,such as post-seismic slip,slow slip and pre-seismic slip events,is a limited lowfrequency deformation that can last for hours to months,in contrast to a sudden slip on a fault caused by earthquakes.Continuous Global Positioning System(CGPS),one of the most common geodetic techniques for continuously monitoring crustal deformation,is capable of capturing transient deformation signals.A critical point in characterizing transient deformation signals is the development of extracting and deciphering transient deformation signals from a huge and messy data set of position time series.Principal Component Analysis(PCA),one of the data-driven methods,has been employed to derive transient deformation signals from position time series combing with Kalman filtering.Independent Component Analysis(ICA)performs well in recovering and separating the sources of observed data,however,it is rarely used in extracting transient deformation signals.We aim to decompose the transient deformation signals from the daily GPS observation deployed in Akutan Island from 2007 to 2015 with the ICA method and obtain the spatiotemporal responses to the source signals of transient deformation.Our results indicate that ICA method can also characterize effectively transient deformation signals spatially and temporally.Additionally,the independent relationship between sources obtained by ICA allows for flexibility in linearly combining different sources.
基金financially supported by the Lhasa National Observation and Research Station of Geophysics(NORSLS20-03)the National Natural Science Foundation of China(42072243)。
文摘This study analyzed the vertical deformation before and after the 2022 Menyuan Ms6.9 earthquake in Qinghai Province,China,using leveling profiles across faults measured from Minle County in Gansu Province to Menyuan County in Qinghai Province.Our results suggest the following:(1)The amplitude of regional vertical differential motion near the Sunna-Qilian and Lenglongling faults within the Qilian Shan increased before the 2022 Menyuan earthquake.It was accompanied by the emergence of high gradient deformation zones.Deformation at the Tongziba cross-fault leveling site near the Sunan-Qilian fault was considerable.In contrast,deformation at the Daliang cross-fault leveling site near the stepover region(adjacent to the epicenter)between the Lenglongling and Tuolaishan faults was minor.After 2018,vertical deformation at the Tongziba site notably accelerated,while that at the Daliang site was insignificant.(2)After the 2022 Menyuan earthquake,140—150 mm of subsidence deformation occurred near the Daliang site,while the Tongziba site did not experience significant deformation.(3)Vertical deformation before and after the 2022 Menyuan earthquake conforms with the elastic-rebound theory,and the evolution of pre-earthquake deformation was consistent with the strike-slip fault deformation pattern at different seismogenic stages,i.e.,the relative motion near the locked fault in the late seismogenic stage gradually weakened.The characteristics of strain accumulation and release derived from the vertical deformation before and after the Menyuan MS6.9 earthquake help understand the deformation process of earthquake preparation and earthquake precursors.
基金supported by the National Natural Science Foundation of China(31971884,31601300,32172020)USDANIFA(#CA-R-BPS-5411-H)to AJL+1 种基金Natural Science Foundation of Sichuan Province(2022NSFSC1696,2019YJ0415)the National Key Research and Development Program of China(2016YFD0102000)。
文摘The Chinese wheat landrace Kaixianluohanmai(KL)expresses the ph-like phenotype.A major QTL,QPh.sicau-3A(syn.phKL),responsible for this effect has been mapped to chromosome arm 3AL.This study presents some characteristics of homoeologous pairing and recombination induced by phKL.In KL haploids,the level of homoeologous pairing was elevated relative to Ph1 Chinese Spring(CS)haploids.There was a clear preference for A–D pairing and less frequent for A–B and B–D,reflecting the higher levels of affinity between genomes A and D in wheat.The characteristics of pairing were affected by temperature and magnesium ion supplementation.The suitability of phKL for chromosome engineering was tested on three pairs of homoeologues:2Sv-2B,2Sv-2D,and 2RL-2BL.The recombination rates were 1.68%,0.17%,and 0%,respectively.The phKL locus in KL induced a moderate level of homoeologous chromosome pairing and recombination when the Ph1 locus of wheat was present,both in wheat haploids and hexaploids.The Ph1-imposed criteria for chromosome pairing and crossing over were relaxed to some degree,permitting homoeologous crossing over but only between closely related chromosomes;there was no crossing over between more differentiated chromosomes.Therefore,the phKL system(QPh.sicau-3A)can be a useful tool in chromosome engineering of wheat to transfer genes from closely related species with the benefit of reduced genomic chaos generated by the ph1b mutation.
基金supported by a grant from the Education Department of Heilongjiang Province of China,No.12541398
文摘Astragaloside Ⅳ is the main active compound of Astragalus membranaceus. Astragaloside Ⅳ has strong anti-oxidative activities and protective effects against progression of peripheral neuropathy. In this study, we determined whether astragaloside Ⅳ protects retinal ganglion cells(RGC) from oxidative stress injury using the rat RGC-5 cell line. Hydrogen peroxide(H_2O_2) was used to induce oxidative stress injury, with the protective effect of astragaloside Ⅳ examined. Cell Counting Kit-8 and 4′,6-diamidino-2-phenylindole staining showed that astragaloside Ⅳ increased cell survival rate and decreased apoptotic cell number. Flow cytometry showed that astragaloside Ⅳ decreased H_2O_2-induced reactive oxygen species levels. While laser confocal microscopy showed that astragaloside Ⅳ inhibited the H_2O_2-induced decrease of mitochondrial membrane potential. Western blot assay showed that astragaloside Ⅳ reduced cytochrome c release induced by H_2O_2, inhibited Bax and caspase-3 expression, and increased Bcl-2 expression. Altogether, these results indicate that astragaloside Ⅳ has potential protective effects against H_2O_2-induced oxidative stress in retinal ganglion cells.
文摘目的:探讨超声引导下腰丛-坐骨神经阻滞对老年股骨颈骨折患者疼痛、应激反应的影响。方法:回顾性分析96例老年股骨颈骨折患者临床资料,51例全麻患者作为对照组,45例超声引导下腰丛-坐骨神经阻滞联合全麻的患者作为观察组。比较两组麻醉情况、术后疼痛度、应激反应及并发症情况。结果:观察组术后苏醒时间、定向力恢复时间、麻醉起效时间、运动阻滞起效时间、感觉阻滞起效时间均短于对照组(P<0.05)。观察组术后各时间点VAS评分均低于对照组(P<0.05),且两组VAS评分在术后24 h明显降低(P<0.05)。与麻醉前相比,两组麻醉后15 min、术后15 min NE、ALD及PRA水平均升高(P<0.05);与麻醉后15 min相比,两组术后15 min NE、ALD及PRA水平均升高(P<0.05),且观察组上述各时间点NE、ALD及PRA水平均低于对照组(P<0.05)。观察组并发症发生率为8.89%,低于对照组的25.49%(P<0.05)。结论:超声引导下腰丛-坐骨神经阻滞在老年股骨颈骨折手术过程中麻醉效果较好,可提高患者手术镇痛效果,降低应激反应,且安全可靠。
基金supported by the National Natural Science Foundation of China(41372345)
文摘Hainan Island is a seismic active region, where Qiongshan M7.5 earthquake occurred in 1605 and several seismic belts appeared in recent years, especially the NS trending seismic belt (NSB) located in the northeast part of the island. Here is also a magmatic active region. The lava from about 100 volcanoes covered more than 4000 km^2. The latest eruptions occurred on Ma'anling-Lei Huling volcanoes within 10,000 years. The neotectonic movement has been determined by geological method in the island and its adjacent areas. In the paper, the present-day 3D crustal movement is obtained by using Global Positioning System (GPS) data observed from 2009 to 2014 and leveling observations measured in 1970s and 1990s respectively. The results show the horizontal movement is mainly along SEE direction relative to the Eurasian Plate. The velocities are between 4.01 and 6.70 mm/a. The tension rate near the NSB is less than I mm/a. The vertical movement shows the island uplifts as a whole with respect to the reference benchmark Xiuyinggang. The average uplifting rate is 2.4 mm/a. The rates are 2-3 mm/a in the northwest and 3-5mm/a in the northwest. It shows the deformation pattern of the southwest island is upward relative to the northeast, which is different from the result inferred from the coastal change and GPS. Haikou and its adjacent region present a subsidence in a long time. The southern part of the middle segment of the Wangwu-Wenjiao fault uplifts relative to the northern. Meanwhile, the western part uplifts relative to the eastern NSB. The vertical crustal motion and the two normal faults nearly correspond to the terrain. The NSB is located along the Puqiangang-Dazhibo fault, which is assessed as a segmented fault with a dip of 80°-90° and party exposed. The 3D deformations and other studies reveal the present activities of earthquakes, volcanoes and the faults. The small earthquakes will still occur in the NS belt and the volcanoes are not active now.