Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens quickly.Stony hard peach cultivars,however,do not produce large amounts of ethylene,and the frui...Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens quickly.Stony hard peach cultivars,however,do not produce large amounts of ethylene,and the fruit remains firm until fully ripe,thus differing from melting flesh peach cultivars.To identify the key proteins involved in peach fruit ripening,an antibody-based proteomic analysis was conducted.A mega-monoclonal antibody(mAb)library was generated and arrayed on a chip(mAbArray)at a high density,covering~4950 different proteins of peach.Through the screening of peach fruit proteins with the mAbArray chip,differentially expressed proteins recognized by 1587 mAbs were identified,and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry.These proteins included not only important enzymes involved in ethylene biosynthesis,such as ACO1,SAHH,SAMS,and MetE,but also novel factors such as NUDT2.Furthermore,protein–protein interaction analysis identified a metabolon containing SAHH and MetE.By combining the antibody-based proteomic data with the transcriptomic and metabolic data,a mathematical model of ethylene biosynthesis in peach was constructed.Simulation results showed that MetE is an important regulator during peach ripening,partially through interaction with SAHH.展开更多
SPECIAL motor systems are high-efficiency and high-quality electromechanical energy conversion devices suitable for complex operating conditions.As key components,special motor systems are widely used in the fields of...SPECIAL motor systems are high-efficiency and high-quality electromechanical energy conversion devices suitable for complex operating conditions.As key components,special motor systems are widely used in the fields of aerospace,energy,power,transportation,high-end manufacturing,etc.Innovations of fundamental theories and application technologies in special motor systems can promote the progress of related disciplines and fields,which has drawn great attentions from countries all over the world.展开更多
基金supported by the National Key Research and Development Program[2018YFD1000200]the National Natural Science Foundation of China[31872085]the Agricultural Science and Technology Innovation Program(ASTIP)[CAAS-ASTIP-2020-ZFRI].
文摘Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens quickly.Stony hard peach cultivars,however,do not produce large amounts of ethylene,and the fruit remains firm until fully ripe,thus differing from melting flesh peach cultivars.To identify the key proteins involved in peach fruit ripening,an antibody-based proteomic analysis was conducted.A mega-monoclonal antibody(mAb)library was generated and arrayed on a chip(mAbArray)at a high density,covering~4950 different proteins of peach.Through the screening of peach fruit proteins with the mAbArray chip,differentially expressed proteins recognized by 1587 mAbs were identified,and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry.These proteins included not only important enzymes involved in ethylene biosynthesis,such as ACO1,SAHH,SAMS,and MetE,but also novel factors such as NUDT2.Furthermore,protein–protein interaction analysis identified a metabolon containing SAHH and MetE.By combining the antibody-based proteomic data with the transcriptomic and metabolic data,a mathematical model of ethylene biosynthesis in peach was constructed.Simulation results showed that MetE is an important regulator during peach ripening,partially through interaction with SAHH.
文摘SPECIAL motor systems are high-efficiency and high-quality electromechanical energy conversion devices suitable for complex operating conditions.As key components,special motor systems are widely used in the fields of aerospace,energy,power,transportation,high-end manufacturing,etc.Innovations of fundamental theories and application technologies in special motor systems can promote the progress of related disciplines and fields,which has drawn great attentions from countries all over the world.
