Stomatin plays a suppressor role in non-small cell lung cancer metastasis

Objective:Metastasis is one of the key causes of high mortality in lung cancer.Aberrant DNA methylation is a common event in metastatic lung cancer.We aimed to identify new epigenetic regulation of metastasis-associated genes and characterize their effects on lung cancer progression.Methods:We screened genes associated with non-small cell lung cancer(NSCLC)metastasis by integrating datasets from the Gene Expression Omnibus(GEO)database.We obtained epigenetic-regulated candidate genes by analyzing the expression profile of demethylation genes.By overlapping analysis,epigenetically modulated metastasis-associated genes were obtained.Kaplan-Meier plotter(KM plotter)was utilized to assess the overall survival(OS)of stomatin in lung cancer.Immunohistochemistry(IHC)was conducted to determine the association between stomatin and metastasis-associated clinical indicators.Both in vitro and in vivo assays were performed to investigate the potential role of stomatin in metastasis.The regulation mechanisms of transforming growth factorβ1(TGFβ1)on stomatin were determined by Sequenom MassARRAY quantitative methylation and western blot assays.Results:A series of bioinformatic analyses revealed stomatin as the metastasis-associated gene regulated by DNA methylation.The KM plotter analysis showed a positive association between stomatin and the OS of lung cancer.IHC analysis indicated that the decreased stomatin expression is linked with advanced TNM stage.Loss-and gainof-function experiments displayed that stomatin could inhibit the migration and invasion of NSCLC cells.Furthermore,TGFβ1 repressed stomatin expression during epithelial-to-mesenchymal transition(EMT).The negative correlation between stomatin and TGFβ1 was also validated in advanced stage III lung tumor samples.The underlying mechanism by which TGFβ1 inhibits stomatin is due in part to DNA methylation.Conclusions:Our results suggest that stomatin may be a target for epigenetic regulation and can be used to prevent metastatic diseases.

Uranium mineralization formed through multi-stage superposition: Case of the Qianjiadian deposit in Songliao Basin, China

The Qianjiadian uranium deposit is located in the Kailu Depression of Songliao Basin.It is a large-scale in-situ leachable uranium deposit of sandstone type and provides a typical case of the uranium deposits in the Songliao Basin.Here we analyze the impact of oil and gas on reduction alteration,and factors including low grade mineralization.The Qianjiadian uranium deposit is characterized as a typical rolltype deposit with interlayers in oxidized zone.The occurrence of reductive low-permeability sandstone in uranium reservoirs controls the morphology of the uranium-rich orebody.We propose a metallogenic model involving multi-stage superposition characterized by“primary sediment enrichment-interlayer oxidation-superimposed transformation”.Our model would be helpful for formulating guidelines in the exploration for sandstone-type uranium deposits in the Songliao Basin.

刚柔并济的仿生凝胶聚合物电解质助力稳定的锂电池

凝胶聚合物电解质(GPE)因其优良的热稳定性和卓越的电化学性能而具备增强锂离子电池性能的潜力,从而受到越来越多的认可.尽管其具有上述优点,但传统GPE的实际应用通常因其溶胀性和有限的机械强度而受到阻碍.为了解决这些问题,本项研究工作提出了一种通过简单方法构建的刚柔并济的仿生GPE,由聚环氧乙烷(PEO)和聚偏二氟乙烯-六氟丙烯(PVDF-HFP)组成并通过Kevlar纤维织物进行增强.所得的PEO/PVDF-HFP/Kevlar(PPK)GPE表现出2.815 mS cm^(−1)的优异离子电导率和0.571的锂离子迁移数,以及32.59 MPa的超高机械强度.这些特性有助于防止锂枝晶生长并增强LiFePO4电池的电化学性能,从而实现稳定的循环性能.PPK GPE可以为高性能锂离子电池的各种实际应用提供理论基础.

PVDF-HFP-SN-based gel polymer electrolyte for high-performance lithium-ion batteries

Gel polymer electrolytes(GPEs)have attracted extensive attention in lithium-ion batteries due to their high security and excellent electrochemical performance.However,their inferior Li-ion transference number,low room-temperature ionic conductivity,and poor long cycle stability raise challenges in practical applications.Herein,a flexible poly(vinylidene fluoride-cohexafluoropropylene)-butanedinitrile(PVDF-HFP-SN)-based GPE(PSGPE)is synthesized successfully by a general immersion precipitation method.The resultant PSGPEs have numerous connecting pores to ensure sufficient space for liquid electrolytes.Moreover,the reduced crystallinity of PVDF-HFP and the high polarity of SN can reduce the energy barrier of Li-ions shuttling between pores.The synergistic effect possesses a high ionic conductivity of 1.35 mS·cm^(-1)at room temperature with a high Li-ion transference number of 0.69.The PVDF-HFP-SN-based GPE is applied in a LiFePO_(4)/graphite battery,which can realize stable cycling performance for 350 cycles and good rate performance at room temperature.These results demonstrate that the novel PSGPE possesses advantage in simplified production process,which can improve the practicability of gel polymer lithium-ion batteries.

Development and validation of the atopic dermatitis and infant skincare knowledge,attitude,and practice(ADISKAP 1.0)scale

Importance:Preserving skin health is crucial for atopic dermatitis control as well as for the thriving of children.However,a well-developed and validated tool that measures the knowledge,attitude,and practice of skin care is lacking.Objective:To develop and validate the atopic dermatitis and infant skincare knowledge,attitude,and practice(ADISKAP 1.0)scale that measures parental health literacy on atopic dermatitis and skin care.Methods:We conducted a review of the literature,a focus group(two dermatologists and 12 parents),and a panel discussion in order to generate the ADISKAP prototype.Two samples of parents with knowingly superior(dermatologists,n=59)and inferior(general population,n=395)knowledge traits participated in the validation of ADISKAP.Cronbach’s alpha was reported as a measure of internal consistency,and the intraclass correlation coefficient(ICC)was calculated to assess the test-retest validity.The known-groups technique was used to evaluate construct validity.Results:The ADISKAP scale contained 17 items after content and face validity validation.After removing items that displayed poor test-retest reliability(n=4)and construct validity(n=3),12 items were retained in the ADISKAP 1.0.Interpretation:ADISKAP 1.0 is a reliable and valid tool for assessing parental knowledge,attitude,and practice on infantile atopic dermatitis and skin care.

Microtubules composed of α4A undergo curved growth mainly mediated by its core structure

Dear Editor,Microtubules consisting of α/β-tubulin dimers exhibit various shapes in different cell stages and cell types,which are important for their diverse roles in eukaryotic cells.Both α-and β-tubulin comprise multiple genes in vertebrates,e.g.mice have at least seven α-tubulin and eight β-tubulin genes.The distribution and expression of these isotypes vary widely among different tissues and developmental stages.Tubulin α1A is generally expressed in post-mitotic neurons and exhibits a decrease in postnatal and adult stages(Yue et al.,2014),while tubulin α4A is highly expressed in the brain and heart during later stages of development(Yue et al.,2014).

Passively Q-switched erbium doped fiber laser using a gold nanostars based saturable absorber

In this paper, we propose and demonstrate an all-fiber passively Q-switched erbium doped fiber laser （EDFL） by using gold nanostars （GNSs） as a saturable absorber （SA） for the first time, to the best of our knowledge. In comparison with other gold nanomorphologies, GNSs have multiple localized surface plasmon resonances, which means that they can be used to construct wideband ultrafast pulse lasers. By inserting the GNS SA into an EDFL cavity pumped by a 980 nm laser diode, a stable passively Q-switched laser at 1564.5 nm was achieved for a threshold pump power of 40 mW. By gradually increasing the pump power from 40 to 120 mW, the pulse duration decreases from 12.8 to 5.3 its and the repetition rate increases from 10 to 17 kHz. Our results indicate that the GNSs are a promising SA for constructing pulse lasers.

Broadband mid-infrared nonlinear optical modulator enabled by gold nanorods:towards the mid-infrared regime

Mid-infrared pulsed lasers operating around the 3 μm wavelength regime are important for a wide range of applications including sensing, spectroscopy, imaging, etc. Despite the recent advances in technology, the lack of a nonlinear optical modulator operating in the mid-infrared regime remains a significant challenge. Here, we report the third-order nonlinear optical response of gold nanorods(GNRs) ranging from 800 nm to the mid-infrared regime(2810 nm) enabled by their size and overlapping behavior-dependent longitudinal surface plasmon resonance. In addition, we demonstrate a wavelength-tunable Er3+-doped fluoride fiber laser modulated by GNRs, which can deliver pulsed laser output, with the pulse duration down to 533 ns, tunable wavelength ranging from 2760.2 to 2810.0 nm, and spectral 3 d B bandwidth of about 1 nm. The experimental results not only validate the GNRs’ robust mid-infrared nonlinear optical response, but also manifest their application potential in high-performance broadband optoelectronic devices.