Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells(PBMCs)expressing ectopic hCG,and hCG-activated PBMCs

Objective:To compare the effect of human chorionic gonadotropin(hCG)-producing peripheral blood mononuclear cells(PBMCs)and PBMCs activated by hCG in vitro and expressions of related immune genes in mouse implantation.Methods:hCG-producing PBMCs(transfected PBMC)and PBMCs activated by hCG in vitro were introduced into isolated mouse endometrial cells,while cell cultures were divided into four groups:the control,PBMC,transfected,and activated PBMC groups.The expression of studied genes(IL-1β,IL-6,Lif,and Vegf)was evaluated and blastocyst attachment on the cocultured cells(isolated endometrial cells and PBMC cells)was monitored in all four groups.Results:Data showed that expression decreased in the PBMC group compared to the treated PBMC(transfected and activated PBMCs)and increased in transfected PBMC compared to the activated PBMC.Attachment and migration of blastocysts were dramatically enhanced in the transfected PBMC group compared to the activated PBMC group(P<0.05).Conclusions:Use of hCG-producing PBMCs(transfected PBMC)has more influence on endometrial receptivity.

Relationship study between platelet count and stage and grade of renal cell carcinoma in indoor patients

Objective:Thrombocytosis has been reported in many types of malignancies and has been studied as a prognostic factor.The aim of this survey is to investigate the relation between platelet count and stage and grade of tumor in indoor patients with renal cell carcinoma(RCC)in order to evaluate the prognostic value of thrombocytosis.Methods:In a descriptive and retrospective survey 82 patients treated by radical nephrectomy for RCC were enrolled.In all cases TNM stage,Fuhrman grade,invasion and platelet count were recorded and entered in SPSS software for analysis.Results:In this study,76 patients(92.7 %)with normal platelet and 6 patients(7.3 %)with thrombocytosis were studied.In this survey there was no significant correlation between the thrombocytosis and pathological stage in all patients,both genders and various age groups.In addition,the correlation between thrombocytosis and nuclear grade was investigated and a significant correlation between them in all patients and both genders was found.Finally,there was no significant correlation between thrombocytosis and nuclear grade at various age groups.Conclusion:Prognostic indicators that can accurately predict survival rates in patients with RCC can be used to select those patients most likely to benefit from adjuvant therapy.In this survey there was a significant correlation between thrombocytosis and nuclear grade,however,further clinical studies are needed.

Regenerating historical districts through tactical urbanism:A case study of Sarpol neighborhood in Isfahan Province,Iran

This paper examines historical urban fabrics’challenges regarding quality of life and visual disturbances despite their cultural and identity value.Regeneration and socialoriented approaches considering social,economic,and cultural dimensions preserve and enhance these valuable materials.Tactical urbanism,with its people-oriented approach,can address these issues.This study focused on Sarpol in Khomeinishahr County,Isfahan Province of Iran.The city’s urban structure divides into two parts:despite its active social structure and valuable spaces,Sarpol is a historic neighborhood with significant deterioration.We followed Corbin and Strauss’s version of Grounded Theory in the qualitative research methodology.Purposive and snowball sampling methods were employed to select 21 participants from the Sarpol neighborhood for semi-structured interviews.The analysis involved three stages of coding:open,axial,and selective.The themes included religious participation,identity,sustainable social bonds,indigenous dependence,and low environmental quality.The central issue identified was the crucial role of religious and cultural values as a driving force for tactical urbanism,which emerged as the most critical semantic domain related to the research problem.

The antimicrobial susceptibility profile of ESKAPE pathogens from urinary tract infections in a referral laboratory,Northeast Iran

Objective:To assess the antimicrobial susceptibility pattern of ESKAPE pathogens from Neyshabur,Iran during 2013-2015.Methods:A total of 345 isolates including 62 Staphylococcus aureus(S.aureus),38 Enterobacter spp.(including 14 Enterobacter agglomerans,6 Enterobacter aerogenes and other 18 Enterobacter spp.),123 Enterococcus faecium,78 Klebsiella pneumonia,10 Pseudomonas aeruginosa and 34 Acinetobacter baumannii were isolated.The antimicrobial susceptibility pattern of isolates was conducted with Kirby Bauer method.Data were analyzed with SPSS 20.0 software using F-and t-tests.Results:Among S.aureus isolates,the highest resistance was observed against nalidixic acid(81.35%)and cefixime(74.50%).Thirty-three(53.22%)S.aureus isolates were cefoxitin resistant(methicillin-resistant S.aureus).The majority of Enterobacter species was resistant to amikacin(100.00%)and cephalotin(66.60%).Most Enterococcus faecium isolates were resistant to nalidixic acid(89.43%)and amikacin(83.33%),but vancomycin-resistant enterococci isolates were not detected.Moreover,among Klebsiella pneumonia,the highest resistance was observed to nalidixic acid(20.98%)and cotrimoxazole(28.39%).Furthermore,all Pseudomonas aeruginosa isolates were resistant to cefotaxime(100.00%)and majority to nitroforantoin(88.80%).Acinetobacter baumannii isolates showed the highest and the lowest resistance to cefotaxime(100.00%)and cefixime(88.71%),respectively.Conclusions:The prevalence of ESKAPE pathogens from northeast region was low,but majority of them exhibited high rate of antibiotic resistance to common used antimicrobial agents.

Epidemiology of cutaneous leishmaniasis in Neyshabur,Iran from 2010 to 2014

Objective:To determine the prevalence of cutaneous leishmaniasis(CL)in Neyshabur,Northeastern Iran from 2010 to 2014.Methods:In this cross-sectional study,a total of 543 samples from patients aging from 9 months to 74 years were included in Neyshabur,Iran.The sampling of lesions was done using sterile syringes.The slides were stained with Giemsa.The leishman bodies were observed under light microscopy.Results:The highest and lowest prevalence of CL was among 21-30 years(P=0.007)and a range of 51-60 years(P=0.156),respectively.The overall incidence of CL from 2010-2014 was 32.2%(n=175).The CL incidence showed a decline from 2010-2014,but was not significant from 47/129(36.4%)cases in 2010 to 18/56(32.1%)cases in 2014.The CL rate was 101/295(34.2%)in males and 74/248(29.8%)in females(P=0.275).The highest and lowest incidence rates of CL in 2014 have been occurred in July and June with 17(36.9%)and 8(19.0%)positive cases,respectively.Conclusions:The incidence of CL in Neyshabur showed a declining trend during 2010-2014.The most susceptible age for leishmaniasis infection was 21-30 years.Leishmaniasis infection rate was not significantly different between men and women.