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Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a‘Wangshuibai’derived population 被引量:1
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作者 Amir Mohammad Naji Mohammad Moghaddam +6 位作者 Mohammad Reza Ghaffari Hashem Pour Irandoost Laleh Karimi Farsad Seyed Mostafa Pirseyedi Seyed Abolghasem Mohammadi Behzad Ghareyazie mohsen mardi 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第10期625-629,共5页
A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat, have been previously identified in the 'Sumai 3'/'Stoa' population. In this study, we ... A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat, have been previously identified in the 'Sumai 3'/'Stoa' population. In this study, we used a 'Wangshuibai' (resistant)/'Seri82' (susceptible) derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in 'Wangshuibai' at 96 h after inoculation was 2.6 times higher than 'Seri82'. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat. 展开更多
关键词 EST-derived STS markers Qfhs.ndsu-3BS Fusarium head blight WHEAT
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Isolation and Characterization of Novel Microsatellite Markers in Walnut (<i>Juglans regia</i>L.)
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作者 Fatemeh Najafi mohsen mardi +3 位作者 Baratali Fakheri Seyed Moustafa Pirseyedi Nafiseh Mehdinejad Maryam Farsi 《American Journal of Plant Sciences》 2014年第3期409-415,共7页
Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ran... Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively. 展开更多
关键词 Isolation MICROSATELLITE JUGLANS regia L. Polymorphism SSR Markers
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