Objective Tissue uptake and distribution of nano-/microplastics was studied at a single high dose by gavage in vivo.Methods Fluorescent microspheres(100 nm,3μm,and 10μm)were given once at a dose of 200 mg/(kg∙body w...Objective Tissue uptake and distribution of nano-/microplastics was studied at a single high dose by gavage in vivo.Methods Fluorescent microspheres(100 nm,3μm,and 10μm)were given once at a dose of 200 mg/(kg∙body weight).The fluorescence intensity(FI)in observed organs was measured using the IVIS Spectrum at 0.5,1,2,and 4 h after administration.Histopathology was performed to corroborate these findings.Results In the 100 nm group,the FI of the stomach and small intestine were highest at 0.5 h,and the FI of the large intestine,excrement,lung,kidney,liver,and skeletal muscles were highest at 4 h compared with the control group(P<0.05).In the 3μm group,the FI only increased in the lung at 2 h(P<0.05).In the 10μm group,the FI increased in the large intestine and excrement at 2 h,and in the kidney at 4 h(P<0.05).The presence of nano-/microplastics in tissues was further verified by histopathology.The peak time of nanoplastic absorption in blood was confirmed.Conclusion Nanoplastics translocated rapidly to observed organs/tissues through blood circulation;however,only small amounts of MPs could penetrate the organs.展开更多
Objective This study was designed to provide the evidences on the toxicokinetics of microplastics(MPs)and nanoplastics(NPs)in the bodies of mammals.Methods 100 nm,3μm,and 10μm fluorescent polystyrene(PS)beads were a...Objective This study was designed to provide the evidences on the toxicokinetics of microplastics(MPs)and nanoplastics(NPs)in the bodies of mammals.Methods 100 nm,3μm,and 10μm fluorescent polystyrene(PS)beads were administered to mice once by gavage at a dose of 200 mg/kg body weight.The levels and change of fluorescence intensity in samples of blood,subcutaneous fat,perirenal fat,peritesticular fat,cerebrum,cerebellum,testis,and epididymis were measured at 0.5,1,2,and 4 h after administration using an IVIS Spectrum small-animal imaging system.Histological examination,confocal laser scanning,and transmission electron microscope were performed to corroborate the findings.Results After confirming fluorescent dye leaching and impact of pH value,increased levels of fluorescence intensity in blood,all adipose tissues examined,cerebrum,cerebellum,and testis were measured in the 100 nm group,but not in the 3 and 10μm groups except in the cerebellum and testis at 4 h for the 3μm PS beads.The presence of PS beads was further corroborated.Conclusion After a single oral exposure,NPs are absorbed rapidly in the blood,accumulate in adipose tissues,and penetrate the blood-brain/testis barriers.As expected,the toxicokinetics of MPs is significantly size-dependent in mammals.展开更多
Objective We aimed to explore the association between obesity and depression and the role of systemic inflammation in older adults.Methods Adults≥65 years old(n=1,973)were interviewed at baseline in 2018 and 1,459 we...Objective We aimed to explore the association between obesity and depression and the role of systemic inflammation in older adults.Methods Adults≥65 years old(n=1,973)were interviewed at baseline in 2018 and 1,459 were followed up in 2021.General and abdominal obesity were assessed,and serum C-reactive protein(CRP)levels were measured at baseline.Depression status was assessed at baseline and at follow-up.Logistic regression was used to analyze the relationship between obesity and the incidence of depression and worsening of depressive symptoms,as well as the relationship between obesity and CRP levels.The associations of CRP levels with the geriatric depression scale,as well as with its three dimensions,were investigated using multiple linear regressions.Results General obesity was associated with worsening depression symptoms and incident depression,with an odds ratio(OR)[95%confidence interval(CI)]of 1.53(1.13-2.12)and 1.80(1.23-2.63),especially among old male subjects,with OR(95%CI)of 2.12(1.25-3.58)and 2.24(1.22-4.11),respectively;however,no significant relationship was observed between abdominal obesity and depression.In addition,general obesity was associated with high levels of CRP,with OR(95%CI)of 2.58(1.75-3.81),especially in subjects free of depression at baseline,with OR(95%CI)of 3.15(1.97-5.04),and CRP levels were positively correlated with a score of specific dimension(life satisfaction)of depression,P<0.05.Conclusion General obesity,rather than abdominal obesity,was associated with worsening depressive symptoms and incident depression,which can be partly explained by the systemic inflammatory response,and the impact of obesity on depression should be taken more seriously in the older male population.展开更多
基金supported by National Natural Science Foundation of China[grant number U21A20399]Liaoning Revitalization Talents Program[grant number XLYC1802059]+2 种基金the Key R&D Program of Liaoning Province[grant number2019JH2/10300044]the Key Laboratory Program of Liaoning Province[grant number 2018225113]the Key Laboratory Program of Shenyang City[grant number 21-103-0-16]。
文摘Objective Tissue uptake and distribution of nano-/microplastics was studied at a single high dose by gavage in vivo.Methods Fluorescent microspheres(100 nm,3μm,and 10μm)were given once at a dose of 200 mg/(kg∙body weight).The fluorescence intensity(FI)in observed organs was measured using the IVIS Spectrum at 0.5,1,2,and 4 h after administration.Histopathology was performed to corroborate these findings.Results In the 100 nm group,the FI of the stomach and small intestine were highest at 0.5 h,and the FI of the large intestine,excrement,lung,kidney,liver,and skeletal muscles were highest at 4 h compared with the control group(P<0.05).In the 3μm group,the FI only increased in the lung at 2 h(P<0.05).In the 10μm group,the FI increased in the large intestine and excrement at 2 h,and in the kidney at 4 h(P<0.05).The presence of nano-/microplastics in tissues was further verified by histopathology.The peak time of nanoplastic absorption in blood was confirmed.Conclusion Nanoplastics translocated rapidly to observed organs/tissues through blood circulation;however,only small amounts of MPs could penetrate the organs.
基金supported by National Natural Science Foundation of China[grand number U21A20399]Liaoning Revitalization Talents Program[grant number XLYC1802059]+1 种基金the Key R&D Program of Liaoning Province[grant number 2019JH2/10300044]Key Laboratory Program of Liaoning Province[grant number 2018225113]。
文摘Objective This study was designed to provide the evidences on the toxicokinetics of microplastics(MPs)and nanoplastics(NPs)in the bodies of mammals.Methods 100 nm,3μm,and 10μm fluorescent polystyrene(PS)beads were administered to mice once by gavage at a dose of 200 mg/kg body weight.The levels and change of fluorescence intensity in samples of blood,subcutaneous fat,perirenal fat,peritesticular fat,cerebrum,cerebellum,testis,and epididymis were measured at 0.5,1,2,and 4 h after administration using an IVIS Spectrum small-animal imaging system.Histological examination,confocal laser scanning,and transmission electron microscope were performed to corroborate the findings.Results After confirming fluorescent dye leaching and impact of pH value,increased levels of fluorescence intensity in blood,all adipose tissues examined,cerebrum,cerebellum,and testis were measured in the 100 nm group,but not in the 3 and 10μm groups except in the cerebellum and testis at 4 h for the 3μm PS beads.The presence of PS beads was further corroborated.Conclusion After a single oral exposure,NPs are absorbed rapidly in the blood,accumulate in adipose tissues,and penetrate the blood-brain/testis barriers.As expected,the toxicokinetics of MPs is significantly size-dependent in mammals.
基金supported by the National Key R&D Program of China[2020 YFC2003000]。
文摘Objective We aimed to explore the association between obesity and depression and the role of systemic inflammation in older adults.Methods Adults≥65 years old(n=1,973)were interviewed at baseline in 2018 and 1,459 were followed up in 2021.General and abdominal obesity were assessed,and serum C-reactive protein(CRP)levels were measured at baseline.Depression status was assessed at baseline and at follow-up.Logistic regression was used to analyze the relationship between obesity and the incidence of depression and worsening of depressive symptoms,as well as the relationship between obesity and CRP levels.The associations of CRP levels with the geriatric depression scale,as well as with its three dimensions,were investigated using multiple linear regressions.Results General obesity was associated with worsening depression symptoms and incident depression,with an odds ratio(OR)[95%confidence interval(CI)]of 1.53(1.13-2.12)and 1.80(1.23-2.63),especially among old male subjects,with OR(95%CI)of 2.12(1.25-3.58)and 2.24(1.22-4.11),respectively;however,no significant relationship was observed between abdominal obesity and depression.In addition,general obesity was associated with high levels of CRP,with OR(95%CI)of 2.58(1.75-3.81),especially in subjects free of depression at baseline,with OR(95%CI)of 3.15(1.97-5.04),and CRP levels were positively correlated with a score of specific dimension(life satisfaction)of depression,P<0.05.Conclusion General obesity,rather than abdominal obesity,was associated with worsening depressive symptoms and incident depression,which can be partly explained by the systemic inflammatory response,and the impact of obesity on depression should be taken more seriously in the older male population.
