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Exploration of the “Four-Three-Four” Internship and Training Mode in Higher Vocational Colleges Under the Modern Vocational Education System
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作者 Jifeng Liu na han +2 位作者 Xiaojiao Li Jing Wang Xuezhen Feng 《Journal of Contemporary Educational Research》 2024年第3期111-117,共7页
This article mainly introduces the innovative internship and training model of vocational colleges,including five aspects such as“four-party collaboration,three-stage progression,four-way integration,value-added eval... This article mainly introduces the innovative internship and training model of vocational colleges,including five aspects such as“four-party collaboration,three-stage progression,four-way integration,value-added evaluation,and double selection and promotion.”The model aims to improve students’practical skills and professional quality to better adapt to market demand and social development.The article also presents the prospects of the future internship and training model,including strengthening cooperation and communication with industry enterprises,focusing on students’personalized development and practical skill cultivation,and establishing a scientific and objective evaluation and feedback mechanism. 展开更多
关键词 Four-party collaboration Three-stage progression Four-way integration Value-added evaluation Double selection and promotion
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电化学二电子氧还原制备过氧化氢研究进展
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作者 文兆宇 韩娜 李彦光 《物理化学学报》 SCIE CAS CSCD 北大核心 2024年第2期1-11,共11页
利用电化学二电子氧还原(2e-Oxygen Reduction Reaction,2e-ORR)方法实现过氧化氢(H_(2)O_(2))的分散式制备,被认为是具有广阔发展前景的技术之一。为实现高的氧还原活性和理想的H_(2)O_(2)选择性,设计高性能2e-ORR电催化剂是该技术的... 利用电化学二电子氧还原(2e-Oxygen Reduction Reaction,2e-ORR)方法实现过氧化氢(H_(2)O_(2))的分散式制备,被认为是具有广阔发展前景的技术之一。为实现高的氧还原活性和理想的H_(2)O_(2)选择性,设计高性能2e-ORR电催化剂是该技术的核心关键。尽管现阶段发展的2e-ORR催化剂已经取得了显著进展,但距离规模化应用仍存在很大的差距。基于此,本文综述了电化学二电子氧还原制备H_(2)O_(2)的最新研究进展,首先简要介绍了反应机理和评价方法,并根据机理分析了影响电催化性能的关键因素。随后围绕贵金属催化剂、碳基催化剂、单原子及分子催化剂的最新研究进展,阐述了提升催化剂活性与H_(2)O_(2)选择性的主要策略。最后,针对电化学二电子氧还原制备H_(2)O_(2)体系面临的关键问题和挑战,对该领域未来的发展趋势进行了展望。 展开更多
关键词 电化学 二电子氧还原反应 过氧化氢 催化剂 选择性
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Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury 被引量:2
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作者 Yuhui Kou Yusong Yuan +3 位作者 Qicheng Li Wenyong Xie Hailin Xu na han 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第8期1822-1827,共6页
Macrophages play an important role in peripheral nerve regeneration,but the specific mechanism of regeneration is still unclear.Our preliminary findings indicated that neutrophil peptide 1 is an innate immune peptide ... Macrophages play an important role in peripheral nerve regeneration,but the specific mechanism of regeneration is still unclear.Our preliminary findings indicated that neutrophil peptide 1 is an innate immune peptide closely involved in peripheral nerve regeneration.However,the mechanism by which neutrophil peptide 1 enhances nerve regeneration remains unclear.This study was designed to investigate the relationship between neutrophil peptide 1 and macrophages in vivo and in vitro in peripheral nerve crush injury.The functions of RAW 264.7 cells we re elucidated by Cell Counting Kit-8 assay,flow cytometry,migration assays,phagocytosis assays,immunohistochemistry and enzyme-linked immunosorbent assay.Axonal debris phagocytosis was observed using the CUBIC(Clear,Unobstructed Brain/Body Imaging Cocktails and Computational analysis)optical clearing technique during Wallerian degeneration.Macrophage inflammatory factor expression in different polarization states was detected using a protein chip.The results showed that neutrophil peptide 1 promoted the prolife ration,migration and phagocytosis of macrophages,and CD206 expression on the surfa ce of macrophages,indicating M2 polarization.The axonal debris clearance rate during Wallerian degeneration was enhanced after neutrophil peptide 1 intervention.Neutrophil peptide 1 also downregulated inflammatory factors interleukin-1α,-6,-12,and tumor necrosis factor-αin invo and in vitro.Thus,the results suggest that neutrophil peptide 1 activates macrophages and accelerates Wallerian degeneration,which may be one mechanism by which neutrophil peptide 1 enhances peripheral nerve regeneration. 展开更多
关键词 axonal debris inflammatory factors MACROPHAGES neutrophil peptide 1 peripheral nerve injury peripheral nerve regeneration RAW 264.7 cells sciatic nerve Wallerian degeneration
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FK506 contributes to peripheral nerve regeneration by inhibiting neuroinflammatory responses and promoting neuron survival
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作者 Yuhui Kou Zongxue Jin +3 位作者 Yusong Yuan Bo Ma Wenyong Xie na han 《Neural Regeneration Research》 SCIE CAS 2025年第7期2108-2115,共8页
FK506(Tacrolimus)is a systemic immunosuppressant approved by the U.S.Food and Drug Administration.FK506 has been shown to promote peripheral nerve regeneration,however,its precise mechanism of action and its pathways ... FK506(Tacrolimus)is a systemic immunosuppressant approved by the U.S.Food and Drug Administration.FK506 has been shown to promote peripheral nerve regeneration,however,its precise mechanism of action and its pathways remain unclear.In this study,we established a rat model of sciatic nerve injury and found that FK506 improved the morphology of the injured sciatic nerve,increased the numbers of motor and sensory neurons,reduced inflammatory responses,markedly improved the conduction function of the injured nerve,and promoted motor function recovery.These findings suggest that FK506 promotes peripheral nerve structure recovery and functional regeneration by reducing the intensity of inflammation after neuronal injury and increasing the number of surviving neurons. 展开更多
关键词 FK506 inflammation motor neurons nerve regeneration NEURON peripheral nerve injury sensory neurons
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Local administration of icariin contributes to peripheral nerve regeneration and functional recovery 被引量:10
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作者 Bo Chen Su-ping Niu +7 位作者 Zhi-yong Wang Zhen-wei Wang Jiu-xu Deng Pei-xun Zhang Xiao-feng Yin na han Yu-hui Kou Bao-guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期84-89,共6页
Our previous study showed that systemic administration of the traditional Chinese medicine Epimedium extract promotes peripheral nerve regeneration. Here, we sought to explore the ther- apeutic effects of local admini... Our previous study showed that systemic administration of the traditional Chinese medicine Epimedium extract promotes peripheral nerve regeneration. Here, we sought to explore the ther- apeutic effects of local administration of icariin, a major component of Epimedium extract, on peripheral nerve regeneration. A poly(lactic-co-glycolic acid) biological conduit sleeve was used to bridge a 5 mm right sciatic nerve defect in rats, and physiological saline, nerve growth factor, icariin suspension, or nerve growth factor-releasing microsphere suspension was injected into the defect. Twelve weeks later, sciatic nerve conduction velocity and the number of myelinated fibers were notably greater in the rats treated with icariin suspension or nerve growth factor-releasing microspheres than those that had received nerve growth factor or physiological saline. The effects of icariin suspension were similar to those of nerve growth factor-releasing microspheres. These data suggest that icariin acts as a nerve growth factor-releasing agent, and indicate that local ap- plication of icariin after spinal injury can promote peripheral nerve regeneration. 展开更多
关键词 nerve regeneration peripheral nerve sciatic nerve traditional Chinese medicine ICARIIN sleeve bridging suture nerve growth factor NSFC grants neural regeneration
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Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation 被引量:7
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作者 Feng Xue Er-jun Wu +4 位作者 Pei-xun Zhang Li-ya A Yu-hui Kou Xiao-feng Yin na han 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期104-111,共8页
We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staini... We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvi- ronment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury. 展开更多
关键词 nerve regeneration spinal cord injury spinal cord hemisection biological conduit bonemarrow mesenchymal stem cells stem cells transmission electron microscope cell transplantation neurons nerve fibers NSFC grants neural regeneration
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Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis 被引量:6
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作者 na han Chun-gui Xu +4 位作者 Tian-bing Wang Yu-hui Kou Xiao-feng Yin Pei-xun Zhang Feng Xue 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期90-94,共5页
Electrical stimulation has been shown to accelerate and enhance nerve regeneration in sensory and motor neurons after injury, but there is little evidence that focuses on the varying degrees of fibrosis in the delayed... Electrical stimulation has been shown to accelerate and enhance nerve regeneration in sensory and motor neurons after injury, but there is little evidence that focuses on the varying degrees of fibrosis in the delayed repair of peripheral nerve tissue. In this study, a rat model of sciatic nerve transec- tion injury was repaired with a biodegradable conduit at 1 day, 1 week, 1 month and 2 months after injury, when the rats were divided into two subgroups. In the experimental group, rats were treated with electrical stimuli of frequency of 20 Hz, pulse width 100 ms and direct current voltage of 3 V; while rats in the control group received no electrical stimulation after the conduit operation. His- tological results showed that stained collagen fibers comprised less than 20% of the total operated area in the two groups after delayed repair at both 1 day and 1 week but after longer delays, the collagen fiber area increased with the time after injury. Immunohistochemical staining revealed that the expression level of transforming growth factor ~ (an indicator of tissue fibrosis) decreased at both 1 day and 1 week after delayed repair but increased at both 1 and 2 months after delayed repair. These findings indicate that if the biodegradable conduit repair combined with electrical stimulation is delayed, it results in a poor outcome following sciatic nerve injury. One month after injury, tissue degeneration and distal fibrosis are apparent and are probably the main reason why electrical stimulation fails to promote nerve regeneration after delayed repair. 展开更多
关键词 nerve regeneration peripheral nerve injury electrical stimulation bioabsorbableconduit delayed repair FIBROBLAST collagen fibers transforming growth factor ~ Masson staining NEUROPROTECTION immunohistochemistry NSFC grants neural regeneration
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Intraoperative single administration of neutrophil peptide 1 accelerates the early functional recovery of peripheral nerves after crush injury 被引量:6
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作者 Yu-Song Yuan Su-Ping Niu +6 位作者 Fei Yu Ya-Jun Zhang na han Hao Lu Xiao-Feng Yin Hai-Lin Xu Yu-Hui Kou 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第11期2108-2115,共8页
Neutrophil peptide 1 belongs to a family of peptides involved in innate immunity. Continuous intramuscular injection of neutrophil peptide 1 can promote the regeneration of peripheral nerves, but clinical application ... Neutrophil peptide 1 belongs to a family of peptides involved in innate immunity. Continuous intramuscular injection of neutrophil peptide 1 can promote the regeneration of peripheral nerves, but clinical application in this manner is not convenient. To this end, the effects of a single intraoperative administration of neutrophil peptide 1 on peripheral nerve regeneration were experimentally observed. A rat model of sciatic nerve crush injury was established using the clamp method. After model establishment, a normal saline group and a neutrophil peptide 1 group were injected with a single dose of normal saline or 10 μg/mL neutrophil peptide 1, respectively. A sham group, without sciatic nerve crush was also prepared as a control. Sciatic nerve function tests, neuroelectrophysiological tests, and hematoxylin-eosin staining showed that the nerve conduction velocity, sciatic functional index, and tibialis anterior muscle fiber cross-sectional area were better in the neutrophil peptide 1 group than in the normal saline group at 4 weeks after surgery. At 4 and 8 weeks after surgery, there were no differences in the wet weight of the tibialis anterior muscle between the neutrophil peptide 1 and saline groups. Histological staining of the sciatic nerve showed no significant differences in the number of myelinated nerve fibers or the axon cross-sectional area between the neutrophil peptide 1 and normal saline groups. The above data confirmed that a single dose of neutrophil peptide 1 during surgery can promote the recovery of neurological function 4 weeks after sciatic nerve injury. All the experiments were approved by the Medical Ethics Committee of Peking University People's Hospital, China(approval No. 2015-50) on December 9, 2015. 展开更多
关键词 crush injury defensin 1 gait analysis INTRAOPERATIVE ADMINISTRATION NERVE conduction velocity nervous system NEUTROPHIL PEPTIDE 1 PERIPHERAL NERVE injury PERIPHERAL NERVE regeneration sciatic NERVE tibialis anterior muscle trauma
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Selective electrocatalytic CO2 reduction enabled by SnO2 nanoclusters 被引量:4
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作者 Hui Yang Yang Huang +5 位作者 Jun Deng Yunling Wu na han Chenyang Zha Leigang Li Yanguang Li 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2019年第10期93-96,共4页
The development of high-performance electrocatalysts holds the decisive key to the electrochemical CO2 reduction toward value-added products. Formic acid or formate is a desirable reduction product, but its selective ... The development of high-performance electrocatalysts holds the decisive key to the electrochemical CO2 reduction toward value-added products. Formic acid or formate is a desirable reduction product, but its selective production is often challenging. Tin based-materials have attracted great attention for formate production, and yet their performances are far from satisfactory. In this study, we reported the preparation of SnO2 nanoclusters from the controlled self-polymerization of dopamine together with SnO32-, followed by the mild-temperature calcination. The final product consisted of large primary particles that were further made of small secondary SnO2 nanocrystals. When evaluated as the electrocatalyst for CO2 reduction in 0.5 M NaHCO3, our material exhibited impressive activity, selectivity and stability for the selective CO2 reduction to formate. A peak formate Faradaic efficiency of^73% and large partial current density of 16.3 mA/cm2 was achieved at -0.92 V versus reversible hydrogen electrode. 展开更多
关键词 CO2 REDUCTION reaction ELECTROCATALYSTS FORMATE Tin oxide Selectivity
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Clinical Analysis of Placenta Previa Complicated with Previous Caesarean Section 被引量:8
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作者 Liang-kun Ma na han +2 位作者 Jian-qiu Yang Xu-ming Bian Jun-tao Liu 《Chinese Medical Sciences Journal》 CAS CSCD 2012年第3期129-133,共5页
Objective To investigate the clinical features and treatment of placenta previa complicated with previous caesarean section. Methods The clinical data of 29 patients with placenta previa complicated with a previous ca... Objective To investigate the clinical features and treatment of placenta previa complicated with previous caesarean section. Methods The clinical data of 29 patients with placenta previa complicated with a previous caesarean section (RCS group) admitted in Peking Union Medical College Hospital during a period from 2003 to 2011 were retrospectively reviewed and compared with those of 243 patients with placenta previa without a previous caesarean section (FCS group) during the same period. Results There was no difference in the mean age (28.9±3.6 vs. 28.1±4.5 years) and the average gravidity (2.35±1.48 vs. 2.21±1.53) between RCS group and FCS group (all P>0.05). The RCS group had more preterm births (24.1% vs. 13.2%), complete placenta previa (55.2% vs. 4.9%), placenta accreta (34.5% vs. 2.5%), more blood loss during caesarean section (1412±602 vs. 648±265 mL), blood transfusion (51.7% vs. 4.9%), disseminated intravascular coagulation (13.8% vs. 2.1%), and obstetric hysterectomy (13.8% vs. 0.8%) than the FCS group (all P<0.05). The preterm infant rate (30.0% vs. 13.0%), neonatal asphyxia rate (10.0% vs. 4.9%), and perinatal mortality rate (6.7% vs. 0.4%) of the RCS group were higher than those of the FCS group (all P<0.05). Conclusions More patients had complete placenta previa and placenta accreta, postpartum hemorrhage, transfusion, uterine packing, obstetric hysterectomy, and perinatal morbidity in the placenta previa patients with previous caesarean section. The patient should be informed of the risk and unnecessary first cesarean sections should be avoided. 展开更多
关键词 placenta previa previous caesarean section perinatal complications
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Sleeve bridging of the rhesus monkey ulnar nerve with muscular branches of the pronator teres: multiple amplification of axonal regeneration 被引量:3
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作者 Yu-hui Kou Pei-xun Zhang +6 位作者 Yan-hua Wang Bo Chen na han Feng Xue Hong-bo Zhang Xiao-feng Yin Bao-guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期53-59,共7页
Multiple-bud regeneration, i.e., multiple amplification, has been shown to exist in peripheral nerve regeneration. Multiple buds grow towards the distal nerve stump during proximal nerve fiber regeneration. Our previo... Multiple-bud regeneration, i.e., multiple amplification, has been shown to exist in peripheral nerve regeneration. Multiple buds grow towards the distal nerve stump during proximal nerve fiber regeneration. Our previous studies have verified the limit and validity of multiple ampli- fication of peripheral nerve regeneration using small gap sleeve bridging of small donor nerves to repair large receptor nerves in rodents. The present study sought to observe multiple ampli- fication of myelinated nerve fiber regeneration in the primate peripheral nerve. Rhesus monkey models of distal ulnar nerve defects were established and repaired using muscular branches of the right forearm pronator teres. Proximal muscular branches of the pronator teres were su- tured into the distal ulnar nerve using the small gap sleeve bridging method. At 6 months after suture, two-finger flexion and mild wrist flexion were restored in the ulnar-sided injured limbs of rhesus monkey. Neurophysiological examination showed that motor nerve conduction veloc- ity reached 22.63 _+ 6.34 m/s on the affected side of rhesus monkey. Osmium tetroxide staining demonstrated that the number of myelinated nerve fibers was 1,657 + 652 in the branches of pronator teres of donor, and 2,661 ~ 843 in the repaired ulnar nerve. The rate of multiple amplification of regenerating myelinated nerve fibers was 1.61. These data showed that when muscular branches of the pronator teres were used to repair ulnar nerve in primates, effective regeneration was observed in regenerating nerve fibers, and functions of the injured ulnar nerve were restored to a certain extent. Moreover, multiple amplification was subsequently detected in ulnar nerve axons. 展开更多
关键词 nerve regeneration peripheral nerve rhesus monkey muscular branches of pronator teres ulnar nerve multiple amplification small gap sleeve bridging NSFC grants neural regeneration
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Neural regeneration after peripheral nerve injury repair is a system remodelling process of interaction between nerves and terminal effector 被引量:8
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作者 Pei-xun Zhang Xiao-feng Yin +3 位作者 Yu-hui Kou Feng Xue na han Bao-guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期52-52,共1页
In China, there are approximately 20 million people suffering from peripheral nerve injury and this number is increasing at a rate of 2 million per year. These patients cannot live or work independently and are a heav... In China, there are approximately 20 million people suffering from peripheral nerve injury and this number is increasing at a rate of 2 million per year. These patients cannot live or work independently and are a heavy responsibility on both family and society because of extreme disability and dysfunction caused by peripheral nerve injury (PNI). Thus, repair of PNI has become a major public health issue in China. 展开更多
关键词 PNI Neural regeneration after peripheral nerve injury repair is a system remodelling process of interaction between nerves and terminal effector
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Genetic Characteristics of 2009 Pandemic H1N1 Influenza A Viruses Isolated from China's Mainland 被引量:4
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作者 Jiu-ru Zhao Yong-dong Li +13 位作者 Li-min Pan na Zhu Hong-xia Ni Guo-zhang Xu Yong-zhong Jiang Xi-xiang Huo Jun-qiang Xu han Xia na han Shuang Tang Zhong Zhang Zheng Kou Simon Rayner Tian-xian Li 《Virologica Sinica》 CAS CSCD 2011年第6期418-427,共10页
A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK cell cultu... A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK cell culture, 57 Influenza A Pandemic (H1N1) viruses were isolated and submitted for whole genome sequencing. A total of 39 HA sequences, 52 NA sequences, 36 PB2 sequences, 31 PB1 sequences, 40 PA sequences, 48 NP sequences, 51 MP sequences and 36 NS sequences were obtained, including 20 whole genome sequences. Sequence comparison revealed they shared a high degree of homology (96%-99%) with known epidemic strains (A/Califomia/04/2009(H1N1). Phylogenetic analysis showed that although the sequences were highly conserved, they clustered into a small number of groups with only a few distinct strains. Site analysis revealed three substitutions at loop 220 (221-228) of the HA receptor binding site in the 39 HA sequences: A/Hubei/86/2009 PKVRDQEG→PKVRDQEA, A/Zhejiang/08/2009 PKVRDQEG→PKVRDQER, A/Hubei/75/2009 PKVRDQEG→PKVRDQGG, the A/Hubei/75/2009 was isolated from an acute case, while the other two were from patients with mild symptoms. Other key sites such as 119, 274, 292 and 294 amino acids of NA protein,627 of PB2 protein were conserved. Meanwhile, all the M2 protein sequences possessed the Ser32Asn mutation, suggesting that these viruses were resistant to adamantanes. Comparison of these sequences with other H1N1 viruses collected from the NCBI database provides insight into H1N1 transmission and circulation patterns. 展开更多
关键词 H1N1 Phylogenetic analysis Key sites MUTATIONS
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Reinnervation of spinal cord anterior horn cells after median nerve repair using transposition with other nerves 被引量:2
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作者 Yu-Song Yuan Su-Ping Niu +8 位作者 You-Lai Yu Pei-Xun Zhang Xiao-Feng Yin na han Ya-Jun Zhang Dian-Ying Zhang Hai-Lin Xu Yu-Hui Kou Bao-Guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第4期699-705,共7页
Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair dis... Our previous studies have confirmed that during nerve transposition repair to injured peripheral nerves, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively repair distal nerve and target muscle tissue and restore muscle motor function. To observe the effect of nerve regeneration and motor function recovery after several types of nerve transposition for median nerve defect(2 mm), 30 Sprague-Dawley rats were randomly divided into sham operation group, epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group. Three months after nerve repair, the wrist flexion test was used to evaluate the recovery of wrist flexion after regeneration of median nerve in the affected limbs of rats. The number of myelinated nerve fibers, the thickness of myelin sheath, the diameter of axons and the cross-sectional area of axons in the proximal and distal segments of the repaired nerves were measured by osmic acid staining. The ratio of newly produced distal myelinated nerve fibers to the number of proximal myelinated nerve fibers was calculated. Wet weights of the flexor digitorum superficialis muscles were measured. Muscle fiber morphology was detected using hematoxylin-eosin staining. The cross-sectional area of muscle fibers was calculated to assess the recovery of muscles. Results showed that wrist flexion function was restored, and the nerve grew into the distal effector in all three nerve transposition groups and the epineurial neurorrhaphy group. There were differences in the number of myelinated nerve fibers in each group. The magnification of proximal to distal nerves was 1.80, 3.00, 2.50, and 3.12 in epineurial neurorrhaphy group, musculocutaneous nerve transposition group, medial pectoral nerve transposition group, and radial nerve muscular branch transposition group, respectively. Nevertheless, axon diameters of new nerve fibers, cross-sectional areas of axons, thicknesses of myelin sheath, wet weights of flexor digitorum superficialis muscle and cross-sectional areas of muscle fibers of all three groups of donor nerves from different anterior horn motor neurons after nerve transposition were similar to those in the epineurial neurorrhaphy group. Our findings indicate that donor nerve translocation from different anterior horn motor neurons can effectively repair the target organs innervated by the median nerve. The corresponding spinal anterior horn motor neurons obtain functional reinnervation and achieve some degree of motor function in the affected limbs. 展开更多
关键词 NERVE REGENERATION TRANSPOSITION repair MEDIAN NERVE functional remodeling muscle atrophy surgical intervention peripheral NERVE injury neural REGENERATION
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Molecular Characterization of China Human Rabies Vaccine Strains 被引量:2
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作者 Xiaoyan Tao na han +3 位作者 Zhenyang Guo Qing Tang Simon Rayner Guodong Liang 《Virologica Sinica》 SCIE CAS CSCD 2013年第2期116-123,共8页
To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavi... To understand the molecular characteristics of China human rabies vaccine strains, we report the full-length genome of the aG strain and present a comprehensive analysis of this strain and almost all available lyssavirus genomes (58 strains) from GenBank (as of Jan 6, 2011). It is generally considered that the G protein plays a predominant role in determining the pathogenicity of the virus, to this end we predicted the tertiary structure of the G protein of aG strain, CTN 181 strain and wild type strain HN 10 based on the crystal structure of Vesicular stomatitis virus (VSV) G. The predicted RABV G structure has a similar topology to VSV G and the ectodomain can be divided into 4 distinct domains DI - DIV. By mapping the characterized mutations to this structure between China vaccine strains and their close street strains, we speculate that the G303(P-H) mutations of CTN181 and HN10 causing D II 3D change may be associated with the attenuated virulence in both strains. Specifically, the two signature mutations (G165P and G231P) in the aG strain are withinβsheets, suggesting that both sites are of structural importance. 展开更多
关键词 Rabies virus LYSSAVIRUS GENOME GLYCOPROTEIN
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Identification of four differentially expressed genes associated with acute and chronic spinal cord injury based on bioinformatics data 被引量:1
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作者 Su-Ping Niu Ya-Jun Zhang +3 位作者 na han Xiao-Feng Yin Dian-Ying Zhang Yu-Hui Kou 《Neural Regeneration Research》 SCIE CAS CSCD 2021年第5期865-870,共6页
Complex pathological changes occur during the development of spinal cord injury(SCI),and determining the underlying molecular events that occur during SCI is necessary for the development of promising molecular target... Complex pathological changes occur during the development of spinal cord injury(SCI),and determining the underlying molecular events that occur during SCI is necessary for the development of promising molecular targets and therapeutic strategies.This study was designed to explore differentially expressed genes(DEGs)associated with the acute and chronic stages of SCI using bioinformatics analysis.Gene expression profiles(GSE45006,GSE93249,and GSE45550)were downloaded from the Gene Expression Omnibus database.SCI-associated DEGs from rat samples were identified,and Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed.In addition,a protein-protein interaction network was constructed.Approximately 66 DEGs were identified in GSE45550 between 3–14 days after SCI,whereas 2418 DEGs were identified in GSE450061–56 days after SCI.Moreover,1263,195,and 75 overlapping DEGs were identified between these two expression profiles,3,7/8,and 14 days after SCI,respectively.Additionally,16 overlapping DEGs were obtained in GSE450061–14 days after SCI,including Pank1,Hn1,Tmem150c,Rgd1309676,Lpl,Mdh1,Nnt,Loc100912219,Large1,Baiap2,Slc24a2,Fundc2,Mrps14,Slc16a7,Obfc1,and Alpk3.Importantly,3882 overlapping DEGs were identified in GSE932491–6 months after SCI,including 3316 protein-coding genes and 567 long non-coding RNA genes.A comparative analysis between GSE93249 and GSE45006 resulted in the enrichment of 1135 overlapping DEGs.The significant functions of these 1135 genes were correlated with the response to the immune effector process,the innate immune response,and cytokine production.Moreover,the biological processes and KEGG pathways of the overlapping DEGs were significantly enriched in immune system-related pathways,osteoclast differentiation,the nuclear factor-κB signaling pathway,and the chemokine signaling pathway.Finally,an analysis of the overlapping DEGs associated with both acute and chronic SCI,assessed using the expression profiles GSE93249 and GSE45006,identified four overlapping DEGs:Slc16a7,Alpk3,Lpl and Nnt.These findings may be useful for revealing the biological processes associated with SCI and the development of targeted intervention strategies. 展开更多
关键词 BIOINFORMATICS differential expression factor gene immune response INJURY PATHWAYS protein spinal cord
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Comparison of commonly used retrograde tracers in rat spinal motor neurons 被引量:4
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作者 You-lai Yu Hai-yan Li +4 位作者 Pei-xun Zhang Xiao-feng Yin na han Yu-hui Kou Bao-guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第10期1700-1705,共6页
The purpose of this study was to investigate the effect of four fluorescent dyes, True Blue(TB), Fluoro-Gold(FG), Fluoro-Ruby(FR), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate(Di I... The purpose of this study was to investigate the effect of four fluorescent dyes, True Blue(TB), Fluoro-Gold(FG), Fluoro-Ruby(FR), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate(Di I), in retrograde tracing of rat spinal motor neurons. We transected the muscle branch of the rat femoral nerve and applied each tracer to the proximal stump in single labeling experiments, or combinations of tracers(FG-Di I and TB-Di I) in double labeling experiments. In the single labeling experiments, significantly fewer labeled motor neurons were observed after FR labeling than after TB, FG, or Di I, 3 days after tracer application. By 1 week, there were no significant differences in the number of labeled neurons between the four groups. In the double-labeling experiment, the number of double-labeled neurons in the FG-Di I group was not significantly different from that in the TB-Di I group 1 week after tracer application. Our findings indicate that TB, FG, and Di I have similar labeling efficacies in the retrograde labeling of spinal motor neurons in the rat femoral nerve when used alone. Furthermore, combinations of Di I and TB or FG are similarly effective. Therefore, of the dyes studied, TB, FG and Di I, and combinations of Di I with TB or FG, are the most suitable for retrograde labeling studies of motor neurons in the rat femoral nerve. 展开更多
关键词 nerve regeneration tracing efficacy fluorescent tracers retrograde tracing femoral nerve motor neurons
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Investigation of the Evolutionary History of the Lyssaviruses 被引量:1
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作者 Xiaoyan Tao Zhenyang Guo +3 位作者 Hao Li na han Qing Tang Guodong Liang 《Virologica Sinica》 SCIE CAS CSCD 2013年第3期186-189,共4页
Dear Editor, We report the results of evolutionary history estimation of the lyssaviruses based on an analysis of the Glycoprotein (G) sequences gene using the BEAST software package. The most recent common ancestor (... Dear Editor, We report the results of evolutionary history estimation of the lyssaviruses based on an analysis of the Glycoprotein (G) sequences gene using the BEAST software package. The most recent common ancestor (TMRCA) of all the lyssavirus strains was estimated to be approximately 5030 years (95% HPD 3988-6069 years), and there was a significant spread of the rabies virus throughout the world range in the last 200 years, consistent with significant time points in development and migration of human civilizations. 展开更多
关键词 狂犬病病毒 进化史 病毒糖蛋白 软件包
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Repair of long segmental ulnar nerve defects in rats by several different kinds of nerve transposition
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作者 Fei Yu You-Lai Yu +7 位作者 Su-Ping Niu Pei-Xun Zhang Xiao-Feng Yin na han Ya-Jun Zhang Dian-Ying Zhang Yu-Hui Kou Bao-Guo Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第4期692-698,共7页
Multiple regeneration of axonal buds has been shown to exist during the repair of peripheral nerve injury, which confirms a certain repair potential of the injured peripheral nerve. Therefore, a systematic nerve trans... Multiple regeneration of axonal buds has been shown to exist during the repair of peripheral nerve injury, which confirms a certain repair potential of the injured peripheral nerve. Therefore, a systematic nerve transposition repair technique has been proposed to treat severe peripheral nerve injury. During nerve transposition repair, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively grow into the repaired distal nerve and target muscle tissues, which is conducive to the recovery of motor function. The aim of this study was to explore regeneration and nerve functional recovery after repairing a long-segment peripheral nerve defect by transposition of different donor nerves. A long-segment(2 mm) ulnar nerve defect in Sprague-Dawley rats was repaired by transposition of the musculocutaneous nerve, medial pectoral nerve, muscular branches of the radial nerve and anterior interosseous nerve(pronator quadratus muscle branch). In situ repair of the ulnar nerve was considered as a control. Three months later, wrist flexion function, nerve regeneration and innervation muscle recovery in rats were assessed using neuroelectrophysiological testing, osmic acid staining and hematoxylin-eosin staining, respectively. Our findings indicate that repair of a long-segment ulnar nerve defect with different donor nerve transpositions can reinnervate axonal function of motor neurons in the anterior horn of spinal cord and restore the function of affected limbs to a certain extent. 展开更多
关键词 NERVE REGENERATION NERVE TRANSPOSITION REPAIR conical SLEEVE small gap SLEEVE bridging ULNAR NERVE target organ muscle NERVE reinnervation neural REGENERATION
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The progress and related pharmacological mechanism of hyperlipidemia treatment 被引量:1
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作者 Zhaohui Ren Jianxiu Zhai +2 位作者 na han Zhihui Liu Jun Yin 《Asian Journal of Traditional Medicines》 2019年第6期268-279,共12页
Hyperlipidemia(HLP)is a long-known risk factor for a series of cardiovascular and cerebrovascular diseases,such as atherosclerosis,coronary heart disease,hypertension and so on.With its increasing incidence,the preven... Hyperlipidemia(HLP)is a long-known risk factor for a series of cardiovascular and cerebrovascular diseases,such as atherosclerosis,coronary heart disease,hypertension and so on.With its increasing incidence,the prevention and treatment of HLP has also become an important research topic around the world.Traditional Chinese medicine(TCM)has been used to treat HLP for a long time with its better therapeutic efficacy,less side effects and wider indications compared with western medicine.In this review,we summarized the pathogenesis of HLP,the research situation of disease-related targets,and the treatment progress of TCM by conducting literature research and sorting out relevant information,in order to provide reference for further research of HLP and prevent the occurrence of cardiovascular and cerebrovascular diseases. 展开更多
关键词 HLP TCM TARGETS treatment progress PATHOGENESIS
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