Clinical analysis of colistin sulfate in the treatment of pneumonia caused by carbapenem-resistant Gram-negative bacteria

BACKGROUND Multidrug-resistant Gram-negative bacteria,exacerbated by excessive use of antimicrobials and immunosuppressants,are a major health threat.AIM To study the clinical efficacy and safety of colistin sulfate in the treatment of carbapenem-resistant Gram-negative bacilli-induced pneumonia,and to provide theoretical reference for clinical diagnosis and treatment.METHODS This retrospective analysis involved 54 patients with Gram-negative bacilli pneumonia admitted to intensive care unit of The General Hospital of the Northern Theater Command of the People's Liberation Army of China from August 2020 to June 2022.After bacteriological culture,the patients'airway secretions were collected to confirm the presence of Gram-negative bacilli.The patients were divided into the experimental and control groups according to the medication used.The research group consisted of 28 patients who received polymyxin sulfate combined with other drugs through intravenous,nebulization,or intravenous combined with nebulization,with a daily dosage of 1.5–3.0 million units.The control group consisted of 26 patients who received standard dosages of other antibiotics(including sulbactam sodium for injection,cefoperazone sodium sulbactam for injection,tigecycline,meropenem,or vaborbactam).RESULTS Of the 28 patients included in the research group,26 patients showed improvement,treatment was ineffective for two patients,and one patient died,with the treatment efficacy rate of 92.82%.Of the 26 patients in the control group,18 patients improved,treatment was ineffective for eight patients,and two patients died,with the treatment efficacy rate of 54.9%;significant difference was observed between the two groups(P<0.05).The levels of white blood cell(WBC),procalcitonin(PCT),and C-reactive protein(CRP)in both groups were significantly lower after treatment than before treatment(P<0.05),and the levels of WBC,PCT,and CRP in the research group were significantly lower than those in the control group(P<0.05).Compared with before treatment,there were no significant changes in aspartate aminotransferase,creatinine,and glomerular filtration rate in both groups,while total bilirubin and alanine aminotransferase decreased after treatment(P<0.05)with no difference between the groups.In patients with good clinical outcomes,the sequential organ failure assessment(SOFA)score was low when treated with inhaled polymyxin sulfate,and specific antibiotic treatment did not improve the outcome.Sepsis and septic shock as well as a low SOFA score were independent factors associated with good clinical outcomes.CONCLUSION Polymyxin sulfate has a significant effect on the treatment of patients with multiple drug-resistant Gram-negative bacilli pneumonia and other infections in the lungs and is safe and reliable.Moreover,the administration route of low-dose intravenous injection combined with nebulization shows better therapeutic effects and lower adverse reactions,providing new ideas for clinical administration.

Correlation between TGFβ1 Gene Polymorphism and Asthma in Baise, Guangxi Children

Objective: This research was to study the correlation between the rs1800469, rs1800470, rs2241712, rs224171 and rs4803455 of TGFβ1 gene and asthma in Baise, Guangxi children. This research also studied the relationship between serum concentration of TGFβ1 and childhood asthma. Method: From June 2022 to December 2023, 121 children had physical examination in affiliated Hospital of Youjiang Medical University for Nationalities were selected as control group and 118 children suffered from asthma in affiliated Hospital of Youjiang Medical University for Nationalities during the same period were selected as asthma group. Result: There was no correlation between rs1800469, rs1800470, rs2241712, rs2241715, rs4803455 and asthma in Baise, Guangxi children. Linkage disequilibrium analysis showed that there were strong linkage disequilibrium among rs1800469, rs1800470, rs2241712, rs2241715 and rs4803455. Their haplotypes had no significant correlation with childhood asthma. The serum concentration of TGFβ1 in asthma group was lower than that in control group (p β1 had no significant relationship with the genotypes of rs1800469, rs1800470, rs2241712, rs2241715 and rs4803455.

Development of a new 17-item Asthenopia Survey Questionnaire using Rasch analysis

AIM:To develop the 17-item Asthenopia Survey Questionnaire(ASQ)-17 by Rasch analysis,and to generate a predictiveness score.METHODS:Totally 739 participants were recruited and 680 were involved in the result analysis in this prospective,cross-sectional study.Three rounds of Rasch analysis were used to analyze the psychometric characteristics of items and options.RESULTS:Phase 1 assessed the original ASQ-19,adjusted the item scoring mode to a four-point Likert response rating scale and combined the 18th and 19th items into a new item.Phase 2 deleted the 11th item.Phases 3 and 4 assessed the new ASQ-17.All the evaluation indexes of ASQ-17 were acceptable.The Infit and Outfit MnSq values of items were 0.67-1.48,the variance explained by the principal component and the unexplained variance explained by the first contrast were 53.90%-59.40%and 1.50-1.80 in three dimensions.The curve peaks of scores in each dimension were separated and in the same order.The PSR and PSI values were 2.80 and 0.89,respectively.The mean scores of dimensions A(9.5±4.1 vs 3.5±3.2),B(7.3±3.3 vs 2.5±2.7),C(4.3±2.2 vs 1.4±2.0)and total(21.1±8.1 vs 7.4±7.0)in asthenopia participants were significantly higher than those without asthenopia(all P<0.001).The area under the curve in two groups was 0.899(P<0.001).Youden’s index was up to the maximum value of 0.784 when the cut-off value was 12.5.CONCLUSION:ASQ-17 has stronger option sorting and suitability than ASQ-19.It is an effective assessment tool for asthenopia with an optimal cut-off threshold value of 12.5,which is suitable for diagnosis and curative effect evaluation.

Altered Iron-Mediated Metabolic Homeostasis Governs the Efficacy and Toxicity of Tripterygium Glycosides Tablets Against Rheumatoid Arthritis

Rheumatoid arthritis(RA),a globally increasing autoimmune disorder,is associated with increased disability rates due to the disruption of iron metabolism.Tripterygium glycoside tablets(TGTs),a Tripterygium wilfordii Hook.f.(TwHF)-based therapy,exhibit satisfactory clinical efficacy for RA treatment.However,drug-induced liver injury(DILI)remains a critical issue that hinders the clinical application of TGTs,and the molecular mechanisms underlying the efficacy and toxicity of TGTs in RA have not been fully elucidated.To address this problem,we integrated clinical multi-omics data associated with the anti-RA efficacy and DILI of TGTs with the chemical and target profiling of TGTs to perform a systematic network analysis.Subsequently,we identified effective and toxic targets following experimental validation in a collagen-induced arthritis(CIA)mouse model.Significantly different transcriptome–protein–metabolite profiles distinguishing patients with favorable TGTs responses from those with poor outcomes were identified.Intriguingly,the clinical efficacy and DILI of TGTs against RA were associated with metabolic homeostasis between iron and bone and between iron and lipids,respectively.Particularly,the signal transducer and activator of transcription 3(STAT3)–hepcidin(HAMP)/lipocalin 2(LCN2)–tartrate-resis tant acid phosphatase type 5(ACP5)and STAT3–HAMP–acyl-CoA synthetase long-chain family member 4(ACSL4)–lysophosphatidylcholine acyltransferase 3(LPCAT3)axes were identified as key drivers of the efficacy and toxicity of TGTs.TGTs play dual roles in ameliorating CIA-induced pathology and in inducing hepatic dysfunction,disruption of lipid metabolism,and hepatic lipid peroxidation.Notably,TGTs effectively reversed“iron–bone”disruptions in the inflamed joint tissues of CIA mice by inhibiting the STAT3–HAMP/LCN2–ACP5 axis,subsequently leading to“iron–lipid”disturbances in the liver tissues via modulation of the STAT3–HAMP–ACSL4–LPCAT3 axis.Additional bidirectional validation experiments were conducted using MH7A and AML12 cells to confirm the bidirectional regulatory effects of TGTs on key targets.Collectively,our data highlight the association between iron-mediated metabolic homeostasis and the clinical efficacy and toxicity of TGT in RA therapy,offering guidance for the rational clinical use of TwHF-based therapy with dual therapeutic and toxic potential.

Neuroinflammation attenuation effects by celastrol and PDIA3 in the amygdala, hippocampus and dorsal raphe nucleus of obese mice

Nowadays,roughly 603.7 million people are bothered by obesity[1].More seriously,obesity brings inflammation to the peripheral and central nervous system,which compromises the comorbidity of obesity,major depression[2],and cognitive deficits[3].Drug competent in the comorbidity is still lacking.In 2015,Liu et al.[4]reported celastrol(CEL)as a powerful anti-obesity agent.In our previous study.

Therapeutic strategies of targeting nonapoptotic regulated cell death (RCD) with smallmolecule compounds in cancer

Regulated cell death(RCD)is a controlled form of cell death orchestrated by one or more cascading signaling pathways,making it amenable to pharmacological intervention.RCD subroutines can be categorized as apoptotic or non-apoptotic and play essential roles in maintaining homeostasis,facilitating development,and modulating immunity.Accumulating evidence has recently revealed that RCD evasion is frequently the primary cause of tumor survival.Several non-apoptotic RCD subroutines have garnered attention as promising cancer therapies due to their ability to induce tumor regression and prevent relapse,comparable to apoptosis.Moreover,they offer potential solutions for overcoming the acquired resistance of tumors toward apoptotic drugs.With an increasing understanding of the underlying mechanisms governing these non-apoptotic RCD subroutines,a growing number of small-molecule compounds targeting single or multiple pathways have been discovered,providing novel strategies for current cancer therapy.In this review,we comprehensively summarized the current regulatory mechanisms of the emerging non-apoptotic RCD subroutines,mainly including autophagy-dependent cell death,ferroptosis,cuproptosis,disulfidptosis,necroptosis,pyroptosis,alkaliptosis,oxeiptosis,parthanatos,mitochondrial permeability transition(MPT)-driven necrosis,entotic cell death,NETotic cell death,lysosome-dependent cell death,and immunogenic cell death(ICD).Furthermore,we focused on discussing the pharmacological regulatory mechanisms of related small-molecule compounds.In brief,these insightful findings may provide valuable guidance for investigating individual or collaborative targeting approaches towards different RCD subroutines,ultimately driving the discovery of novel small-molecule compounds that target RCD and significantly enhance future cancer therapeutics.

基于lncRNA微阵列芯片技术探索间充质干细胞外泌体增强小鼠胰岛β细胞抗低氧损伤的潜在机制

目的探索间充质干细胞(MSC)外泌体提高低氧条件下胰岛β细胞活性的潜在分子机制。方法将小鼠胰岛β细胞分别培养于常氧环境(5%CO_(2),95%空气)、低氧环境(2%O_(2),5%CO_(2),93%N_(2))以及低氧环境添加MSC外泌体(50μg/mL)。采用CCK-8检测试剂盒测定小鼠胰岛β细胞活性、细胞凋亡检测试剂盒分析小鼠胰岛β细胞凋亡情况;通过小鼠Arraystart LncRNA微阵列芯片分析不同培养环境下lncRNA和mRNA的表达情况;以Arraystart LncRNA微阵列芯片检测结果为生物信息学分析数据来源,基于t检验并设置显著性阈值P≤0.05和|Fold Change|≥2为筛选标准,筛选并获得差异表达显著的lncRNA和mRNA,采用Pearson相关系数法分析样本间lncRNA与mRNA表达情况的相关性以明确差异lncRNA的潜在靶基因,进而通过对上述靶基因进行GO和KEGG富集分析,来探究MSC外泌体通过哪条信号通路在低氧条件下增强小鼠胰岛β细胞抗低氧损伤能力。两组间数据比较采用t检验,三组间进行单因素方差分析,然后进行Dunnett's-t多重比较分析。结果CCK-8结果显示,与常氧条件相比,低氧条件下小鼠胰岛β细胞OD值(0.44±0.02比0.53±0.01)下降(t=4.455,P<0.05)。凋亡分析结果显示,与常氧培养相比,低氧条件下小鼠胰岛β细胞凋亡率(33.03%±3.12%比11.27%±2.69%)升高(t=5.289,P<0.01)。低氧培养条件下加入MSC来源外泌体干预后,小鼠胰岛β细胞活性OD值较单纯低氧培养(0.42±0.03比0.33±0.01)升高(P<0.01);小鼠胰岛β细胞凋亡率较低氧培养(15.23%±0.62%比32.63%±0.95%)降低(P<0.01)。利用LncRNA微阵列芯片完成小鼠胰岛β细胞中35293个lncRNA和24881个mRNA表达水平的检测,结果显示,与常氧培养条件相比低氧培养条件可引起1726个lncRNA和1023个mRNA表达差异;与低氧培养条件相比,加入MSC来源外泌体干预后,491个lncRNA和406个mRNA表达差异。对两种不同培养条件下差异lncRNA和mRNA利用韦恩图分别取交集,最终获得均有表达差异的lncRNA 112个、mRNA 60个;进一步相关性分析提示这112个lncRNA存在1582个潜在靶基因;进而对这些靶基因进行GO和KEGG富集分析,结果显示差异表达lncRNA靶基因主要与MAPK、自噬等信号通路等密切相关。结论低氧可引起小鼠胰岛β细胞凋亡,MSC来源外泌体可提高低氧条件下β细胞活性,抑制低氧诱发的小鼠胰岛β细胞凋亡,这可能与lncRNA调控MAPK、自噬等信号通路有关。

我国和欧盟兽药质量监督抽检工作机制对比研究

为更好地完善我国兽药质量监督抽检计划,通过对欧盟药品抽检管理工作机制的研究,本文分析了欧盟和我国兽药监督抽检管理方式的不同,从抽检责任部门、抽检目的、抽检计划内容、抽检计划制定流程、抽检品种的遴选和检测参数的确定等6方面进行了对比分析,探讨我国兽药质量监督抽检工作的管理中可以借鉴的方面。

Effect of Different Treatments on Antioxidative Stability of the Scallop Protein Hydrolysates

Effects of different treatments on the antioxidant activity of scallop protein hydrolysates (SPH) were evaluated using DPPH radical scavenging activity and reducing power. Results showed that the antioxidant activity of SPH had good heating-resistance from 25°C to 65°C. The antioxidant activity of SPH could retain under acidic environment, but rapidly reduced under alkaline conditions. Addition of D-galactose, D-xylose, and D-fructose at 65°C could increase the antioxidant activity of SPH, but no such effect was not observed at this temperature. With the increase of storage time, the antioxidant activity of SPH gradually decreased. Moreover, pepsin digestion treatment slightly reduced the antioxidant activity of SPH, and further trypsin and mixed enzyme (trypsin + chymotrypsin) digestion significantly reduced this activity (p < 0.05). In conclusion, SPH may be used as food ingredients or food supplements in different food fields.

Early Cambrian Mollusc Watsonella crosbyi:A Potential GSSP Index Fossil for the Base of the Cambrian Stage 2

The mollusc Watsonella crosbyi was studied on the basis of material from the lower Cambrian Dahai Member of the Zhujiaqing Formation in eastern Yunnan, China and the Pestrotsvet Formation of the Aldan River region in the Siberian Platform. This fossil had been excessively described under different names in the literature, such as Heraultia varensalensis Cobboid, 1935, H. sibirica Missarzhevsky, 1974, Heraultipegma yunnanensis He and Yang, 1982, H. yannanese He and Yang,1982, Watsonella yunnanensis, W. crosbyi Grabau, 1900, etc. Taxonomic revision shows that other species are junior synonyms of W. crosbyi. Analysis of functional morphology implies that W. crosbyi may be rather a heicionelloid than a rostroconch since its univalved conch is untorted endogastric and lacks a rostrum and a true internal pegma. Replicas of the original microstrnctures preserved on the surfaces of phosphatic internal moulds confirm that the shell consists of two layers. The inner layer is lamello-fibrillar, and the outer layer is spherulitic prismatic. The widespread geographical occurrences in both siliciclastic and carbonate rocks of the late Terreneuvian indicate that W. crosbyi is an important index fossil for global correlations and subdivision of the Cambrian Series I （Terreneuvian）.The first appearance datum （FAD） of W. crosbyi is suggested to be a potential GSSP （Global Boundary Stratotype Section and Point） candidate marker for defining the base of the Cambrian Stage 2.

Apoptosis and its pathway in X gene-transfected HepG_2 cells

AIM： To investigate the effect of hepatitis B virus （HBV） X gene on apoptosis and expressions of apoptosis factors in X gene-transfected HepG2 cells.METHODS： The HBV X gene eukaryon expression vector pcDNA3-X was transiently transfected into HepG2 cells by lipid-media transfection. Untransfected HepG2 and HepG2 transfected with pcDNA3 were used as controls. Expression of HBx in HepG2 was identified by RT-PCR. MTT and TUNEL were employed to measure proliferation and apoptosis of cells in.three groups. Semi-quantified RT-PCR was used to evaluate the expression levels of Fas/FasL, Bax/Bcl-xL, and c-myc in each group.RESULTS： HBV X gene was transfected into HepG2 cells successfully. RT-PCR showed that HBx was only expressed in HepG2/pcDNA3-X cells, but not expressed in HepG2 and HepG2/pcDNA3 cells. Analyzed by MTT, cell proliferationcapacity was obviously lower in HepG2/pcDNA3-X cells （0.08910±0.003164） than in HepG2 （0.14410±0.004927） and HepG2/pcDNA3 cells （0.12150±0.007159） （P〈0.05 and P〈0.01）. Analyzed by TUNEL, cell apoptosis was much more in HepG2/pcDNA3-X cells （980/2 000） than HepG2 （420/2 000）, HepG2/pcDNA3 cells （520/2 000） （P〈0.05 and P〈0.01）. Evaluated by semi-quantified RT-PCR, the expression level of Fas/FasL was significantly higher in HepG2 cells transfected with HBx than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）. Bax/Bcl-xL expression level was also elevated in HepG2/pcDNA3-X cells （P〈0.05 and P〈0.01）. Expression of c-myc was markedly higher in HepG2/pcDNA3-X cells than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）.CONCLUSION： HBV X gene can impair cell proliferation capacity, improve cell apoptosis, and upregulate expression of apoptosis factors. The intervention of HBV X gene on the expression of apoptosis factors may be a possible mechanism responsible for the change in cell apoptosis and proliferation.

HuoguⅠformula prevents steroid-induced osteonecrosis in rats by down-regulating PPARγ expression and activating Wnt/LRP5/β-catenin signaling

OBJECTIVE： To investigate the effects of Huogu I formula on regulation of lipid metabolism in ste- roid-induced osteonecrosis of the femoral head （SONFH） rats and verify our hypothesis that Huogu I formula regulates lipid metabolism by down-regulating peroxisome proliferator-activated receptor gamma （PPARy） expression and activating Wnt signaling pathways. METHODS： Eighty-five rats were divided into four groups： control, model, Huogu 15 g/kg and Huogu 30 g/kg. Six weeks later, animals were anaesthe- tized, femora were dissected for histopathologicalexamination of the osteonecrotic changes and re- pair processes, micro computed tomography （Mi- cro-CT）-based micro-angiography was performed to assess vascularization. Serum lipid levels were detected by haematological examination. The ex- pressions of PPARy, Wnt3a, low density lipoprotein receptor-related protein 5 （LRP5） and 13-catenin were evaluated by immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction analyses. RESULTS： The incidence of osteonecrosis, ratio of empty lacuna, adipose tissue area and adipocyte perimeter in the bone marrow were dramatically lower in the Huogu ~ formula treatment groups. By micro-CT quantification, Huogu ~ formula treat- ment dose-dependently increased vessel volume, vessel surface, percentage of vessel volume and vessel thickness of the femoral heads of SONFH rats. Levels of serum lipid in Huogu 15 g/kg and Huogu 30 g/kg groups reduced significantly. HuoguⅠformula treatment could suppress the ex- pression of PPARy and increase the expressions of Wnt3a, LRP5 and 13-catenin at both protein and mRNA levels. CONCLUSION： The results of our present study highlight the lipid-lowering potential of Huogu Ⅰ formula, and provide further evidence of the in- volvement of the PPARy inhibition and Wnt/LRPS/ 13-catenin signaling activation in the effects of Huogu Ⅰ formula.

CVCC菌(毒)种基因序列管理系统的开发与应用

通过对中国兽医微生物菌种保藏管理中心（CVCC）菌（毒）种基因序列管理模式的研究以及对用户需求的调研,开发了CVCC菌（毒）种基因序列管理系统,实现了菌（毒）种分子生物学特性的数据信息化呈现。由此为菌（毒）种基因序列资源的进一步规范化管理奠定了良好基础,同时提高了基因序列资源的利用率。对微生物领域的科技创新和菌（毒）种资源共享服务质量的提高具有良好的推动作用。

Clinical features and mutations in seven Chinese patients with very long chain acyl-CoA dehydrogenase deficiency

Background:Very long chain acyl-CoA dehydrogenase deficiency(VLCADD)is an inherited metabolic disease caused by deleterious mutations in the ACADVL gene that encodes very long chain acyl-CoA dehydrogenase(VLCAD),and which can present as cardiomyopathy in neonates,as hypoketotic hypoglycemia in infancy,and as myopathy in late-onset patients.Although many ACADVL mutations have been described,no prevalent mutations in the ACADVL gene have been associated with VLCADD.Herein,we report the clinical course of the disease and explore the genetic mutation spectrum in seven Chinese patients with VLCADD.Methods:Seven Chinese patients,from newborn to 17 years old,were included in this study.Tandem mass spectrometry was performed to screen for VLCAD defi ciency.All exons and fl anking introns of the ACADVL gene were analyzed using polymerase chain reaction and direct sequencing.Online analysis tools were used to predict the impact of novel mutations.Results:All cases had elevated serum levels of tetradecanoylcarnitine(C14:1)which is the characteristic biomarker for VLCADD.The phenotype of VLCADD is heterogeneous.Two patients were hospitalized for hypoactivity and hypoglycemia shortly after birth.Three patients showed hepatomegaly and hypoglycemia in infancy.The other two adolescent patients showed initial manifestations of exercise intolerance or rhabdomyolysis.Three of the patients died at the age of 6-8 months.Eleven different mutations in the ACADVL gene in the 7 patients were identified,including seven reported mutations(p.S22X,p.W427X,p.A213T,p.G222R,p.R450H,c.296-297delCA,c.1605+1G>T)and four novel mutations(p.S72F,p.Q100X,p.M437T,p.D466Y).The p.R450H and p.D466Y(14.28%,2/14 alleles)mutations were identifi ed in two alleles respectively.Conclusions:The clinical manifestations were heterogeneous and ACADVL gene mutations were heterozygous in the seven VLCADD Chinese patients.R450H may be a relatively common mutation in Asian populations.The genotype and phenotype had a certain correlation in our patients.

A Novel Improved Bat Algorithm in UAV Path Planning

Path planning algorithm is the key point to UAV path planning scenario.Many traditional path planning methods still suffer from low convergence rate and insufficient robustness.In this paper,three main methods are contributed to solving these problems.First,the improved artificial potential field(APF)method is adopted to accelerate the convergence process of the bat’s position update.Second,the optimal success rate strategy is proposed to improve the adaptive inertia weight of bat algorithm.Third chaos strategy is proposed to avoid falling into a local optimum.Compared with standard APF and chaos strategy in UAV path planning scenarios,the improved algorithm CPFIBA(The improved artificial potential field method combined with chaotic bat algorithm,CPFIBA)significantly increases the success rate of finding suitable planning path and decrease the convergence time.Simulation results show that the proposed algorithm also has great robustness for processing with path planning problems.Meanwhile,it overcomes the shortcomings of the traditional meta-heuristic algorithms,as their convergence process is the potential to fall into a local optimum.From the simulation,we can see also obverse that the proposed CPFIBA provides better performance than BA and DEBA in problems of UAV path planning.

Biomolecular network-based synergistic drug combination discovery:a combination of paeoniflorin and liquiritin alleviates neuropathic pain by inhibiting neuroinflammation via suppressing the chemokine signaling pathway

Dear Editor,Neuropathic pain(NP)resulting from injuries or diseases affecting the somatosensory nervous system is highly prevalent in various pathological conditions.1 Since NP is debilitating and impacts health and the quality of life,there is an urgent need for effective non-addictive new therapies.Traditional Chinese medicine(TCM)has been increasingly used for its benefits in relieving pain in clinics.Wu-Tou Decoction(WTD),which was first described by the famous TCM classic“Jin Gui Yao Lue”,is one of the most effective TCM herbal prescriptions for pain management.A growing body of clinical evidence shows that WTD markedly alleviates different types of NP,such as trigeminal neuralgia,inflammatory pain,and cancer-induced pain,with a total effectiveness of~80%.2 Our previous data demonstrated the analgesic effects of WTD via suppression of glial cell activation and neuroinflammation and further revealed that WTD attenuated NP partially by inhibiting spinal astrocytic IL-1R1/TRAF6/JNK signaling and regulating the glutamatergic system in CA3 in spinal nerve ligation(SNL)-induced NP in vivo.3,4 However,the bioactive compounds(BACs)of WTD that have an effect on NP and the underlying mechanisms remain unclear.

A Novel Improved Artificial Bee Colony and Blockchain-Based Secure Clustering Routing Scheme for FANET

Flying Ad hoc Network(FANET)has drawn significant consideration due to its rapid advancements and extensive use in civil applications.However,the characteristics of FANET including high mobility,limited resources,and distributed nature,have posed a new challenge to develop a secure and ef-ficient routing scheme for FANET.To overcome these challenges,this paper proposes a novel cluster based secure routing scheme,which aims to solve the routing and data security problem of FANET.In this scheme,the optimal cluster head selection is based on residual energy,online time,reputation,blockchain transactions,mobility,and connectivity by using Improved Artificial Bee Colony Optimization(IABC).The proposed IABC utilizes two different search equations for employee bee and onlooker bee to enhance convergence rate and exploitation abilities.Further,a lightweight blockchain consensus algorithm,AI-Proof of Witness Consensus Algorithm(AI-PoWCA)is proposed,which utilizes the optimal cluster head for mining.In AI-PoWCA,the concept of the witness for block verification is also involved to make the proposed scheme resource efficient and highly resilient against 51%attack.Simulation results demonstrate that the proposed scheme outperforms its counterparts and achieves up to 90%packet delivery ratio,lowest end-to-end delay,highest throughput,resilience against security attacks,and superior in block processing time.

Myeloid sarcoma of the colon as initial presentation in acute promyelocytic leukemia:A case report and review of the literature

BACKGROUND Myeloid sarcoma(MS)rarely occurs in acute promyelocytic leukemia(APL)at onset,but it can develop in relapse cases,especially after APL treated with alltrans retinoic acid(ATRA).Therefore little is known about the clinical features and suitable treatment for APL related MS due to the rarity of the disease,although this may be different from the treatment and prognosis of MS in the relapse stage.To our best knowledge,this is the second case report of APL initial presentation as colon MS.CASE SUMMARY A 77-year-old woman complained of intermittent right lower abdominal pain,black stool,and difficult defecation for 2 mo.Physical examination showed diffuse tenderness during deep palpation and an anemic appearance.Laboratory findings showed positivity for fecal occult blood testing;white blood cell count:3.84×109/L;hemoglobin:105 g/L;platelet count:174×109/L;and negativity for tumor markers.Abdominal enhanced computed tomography showed a space occupying lesion in the colon(1.9 cm).Fibrocolonoscopy revealed a polypoid and ulcerated mass measuring 2.5 cm.The tumor was removed.To our surprise,MS was confirmed by immunohistochemistry.PML/RARαfusion gene was detected in colon specimens by fluorescent in situ hybridization and real-time reverse transcription polymerase chain reaction,which was consistent with the bone marrow.She was diagnosed as having APL related MS.A smooth and unobstructed intestinal wall was found by fibrocolonoscopy,and continuous molecular remission was confirmed in both the bone marrow and colon after four courses of ATRA+arsenic trioxide(ATO).ATRA+ATO showed a favorable therapeutic response for both APL and MS.CONCLUSION Early use of ATRA can benefit APL patients,regardless of whether MS is the first or recurrent manifestation.

Theoretical framework construction on care complexity in Chinese hospitals: A grounded theory study

Objectives: This study aims to construct a theoretical framework to analyze risk factors and explore hospital nurses' perspectives on care complexity.Methods: The grounded theory method was adopted,and semi-structured in-depth interviews regarding the understanding of care complexity were conducted among the participants,including 31 nurses and nine doctors.In addition,data were coded and strictly analyzed in accordance with the coding strategy and requirements of grounded theory.Results: Our study reveals three factors that are closely related to care complexity,namely,(1) patient factors,including patients' condition,age,self-care abilities,compliance,social support systems,psy chological conditions,expectations,and requirements;(2) nursing staff factors,including work experiences,education,knowledge and operational skills of caring,and communication skills;and (3) organization and equipment factors,including nursing workforce,nursing workload,support from multidisciplinary teams and ancillary departments,and the conditions of medical and hospital services.Conclusions: This study defines care complexity on the basis of its factors.Care complexity refers to the difficulty of nursing tasks during patient care plan implementation,which are affected by patients,nurses,and other factors in nursing and multisectoral,multidisciplinary cooperation.The framework can be beneficial for nursing education and for the improvement of the quality and efficiency of clinical nursing practice.

自由之思想 崇真之学问——刘川鄂学术研究综论

刘川鄂是湖北文学评论界标杆式领军人物之一,是在中国文学界有着广泛影响的评论家。30多年来,刘川鄂始终秉持追求心灵学术的初心,坚持以人性含量和审美含量作为评判文学作品的双重标准,致力于中国现当代文学文化研究,写出了《张爱玲传》《传奇未完:张爱玲1920-1995》《中国自由主义文学论稿》《世纪转型期的湖北诗歌研究》《从现代到当代》《批评家的左手和右手》等一系列在当代文坛有重要影响的著作。闻名学术研究领域,他是真学问的追求者;享誉文学批评界,他是"左右手"齐力的提出者、倡导者和践行者;作为社会观察家,他是大众文化、社会热点的评论者和新时代教育的探路者。