The dispersion curves of bulk waves propagating in both AlN and ZnO film bulk acoustic resonators(FBARs)are presented to illustrate the mode flip of the thickness-extensional(TE)and 2nd thickness-shear(TSh2)modes.The ...The dispersion curves of bulk waves propagating in both AlN and ZnO film bulk acoustic resonators(FBARs)are presented to illustrate the mode flip of the thickness-extensional(TE)and 2nd thickness-shear(TSh2)modes.The frequency spectrum quantitative prediction(FSQP)method is used to solve the frequency spectra for predicting the coupling strength among the eigen-modes in AlN and ZnO FBARs.The results elaborate that the flip of the TE and TSh2 branches results in novel self-coupling vibration between the small-wavenumber TE and large-wavenumber TE modes,which has never been observed in the ZnO FBAR.Besides,the mode flip leads to the change in the relative positions of the frequency spectral curves about the TE cut-off frequency.The obtained frequency spectra can be used to predict the mode-coupling behaviors of the vibration modes in the AlN FBAR.The conclusions drawn from the results can help to distinguish the desirable operation modes of the AlN FBAR with very weak coupling strength from all vibration modes.展开更多
Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the preven...Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the prevention of heart failure after MI,and miR-106a is considered to be an important regulatory factor in MI.But the specific mechanism remains unknown.In this study,using cultured venous endothelial cells and a rat model of MI,we aimed to identify the potential target genes of miR-106a and discover the mechanisms of inhibiting autophagy and angiogenesis.Methods:We first explored the biological functions of miR-106a on autophagy and angiogenesis on endothelial cells.Then we identified ATG7,which was the down-stream target gene of miR-106a.The expression of miR-106a and ATG7 was investi-gated in the rat model of MI.Results:We found that miR-106a inhibits the proliferation,cell cycle,autophagy and angiogenesis,but promoted the apoptosis of vein endothelial cells.Moreover,ATG7 was identified as the target of miR-106a,and ATG7 rescued the inhibition of autophagy and angiogenesis by miR-106a.The expression of miR-106a in the rat model of MI was decreased but the expression of ATG7 was increased in the infarction areas.Conclusion:Our results indicate that miR-106a may inhibit autophagy and angiogenesis by targeting ATG7.This mechanism may be a potential therapeutic treatment for MI.展开更多
[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shoc...[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shock factor AtHsfAl a and programmed cell death in A. thaliana. [ Method ] Different genotypes of A. thaliana (AtHsfAla-silenced transgenic and wild-type) seedlings were treated at 42 ℃. According to the fragmentation level of fluorogenic substrate Ac-DEVD- pNA, Caspase-3 activity was determined by spectrophotometry. [ Result] After high temperature treatment, Caspase-3 activity in A. thaliana was enhanced signifi-cantly. Caspase-3 activity in AtHsfAla-si/enced transgenic A. thaliana was higher than that in wild-type A. thaliana, which indicated that AtHsfAla could inhibit Caspase-3 activity in A. thaliana under high temperature stress. [ Conclusion] Under high temperature stress, heat shock factor AtHsfAla might exert inhibitory effects on programmed cell death by reducing Caspase-3 activity. This study provided the basis for clarifying the mechanism of stress resistance in plants.展开更多
The investigation focusing on the mechanical behaviors at the microstructural level in composite materials can provide valuable insight into the failure mechanisms at larger scales.A micromechanics damage model which ...The investigation focusing on the mechanical behaviors at the microstructural level in composite materials can provide valuable insight into the failure mechanisms at larger scales.A micromechanics damage model which comprises the coupling of the matrix constitutive model and the cohesive zone(CZM)model at fiber-matrix interfaces is presented to evaluate the transverse tensile damage behaviors of unidirectional(UD)fiber-reinforced polymer(FRP)composites.For the polymeric matrix that exhibits highly non-linear mechanical responses,special focus is paid on the formulation of the constitutive model,which characterizes a mixture of elasticity,plasticity as well as damage.The proposed constitutive model includes the numerical implementation of a fracture plane based ellipse-parabola criterion that is an extension of the classic Mohr-Coulomb criterion,corresponding post-yield flow rule and post-failure degradation rule in the fully implicit integration scheme.The numerical results are in good agreement with experimental measurements.It is found that directly using the matrix properties measured at the ply level to characterize the mechanical responses at the constituent level may bring large discrepancies in homogenized stress-strain responses and dominant failure mechanisms.The distribution of fracture plane angles in matrix is predicted,where it is shown to provide novel insight into the microscopic damage initiation and accumulation under transverse tension.展开更多
[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala (SALK-068042) and wild-type A. thaliana seedl...[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala (SALK-068042) and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology (GO) analysis. Signal transduction pathways, in which differentia|ly expressed genes were involved, were analyzed by pathway analysis. Gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.展开更多
[Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on programmed cell death in Arabidopsis thaliana under cold stress. [ Method] AtHsfAla-silenced transgenic (NT) and wild-type (W...[Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on programmed cell death in Arabidopsis thaliana under cold stress. [ Method] AtHsfAla-silenced transgenic (NT) and wild-type (WT) A. thaliana seedlings were used as experimental materials to induce the formation of callus; the callus were cultured to single cells by suspension culture, subjected to cold stress, stained with DAPI, prepared into cell smears and observed under a fluorescence microscope. [ Result] Under cold stress, cell nucleus of wild-type A. thaliana displayed morphological changes, but no apoptotic bodies were found; apoptotic bodies were observed in AtHsfAla-silenced transgenic A. thaliana cells, and the cytoplasm was remarkably concentrated. [ Conclusion] Under cold stress, heat shock factor AtHsfAla exerted inhibitory effects on programmed cell death in A. thaliana, which was of great significance for clarifying the mechanism of stress responses in plants.展开更多
In this paper,we propose a specific two-layer model consisting of a functionally graded(FG)layer and a piezoelectric semiconductor(PS)layer.Based on the macroscopic theory of PS materials,the effects brought about by ...In this paper,we propose a specific two-layer model consisting of a functionally graded(FG)layer and a piezoelectric semiconductor(PS)layer.Based on the macroscopic theory of PS materials,the effects brought about by the attached FG layer on the piezotronic behaviors of homogeneous n-type PS fibers and PN junctions are investigated.The semi-analytical solutions of the electromechanical fields are obtained by expanding the displacement and carrier concentration variation into power series.Results show that the antisymmetry of the potential and electron concentration distributions in homogeneous n-type PS fibers is destroyed due to the material inhomogeneity of the attached FG layer.In addition,by creating jump discontinuities in the material properties of the FG layer,potential barriers/wells can be produced in the middle of the fiber.Similarly,the potential barrier configuration near the interface of a homogeneous PS PN junction can also be manipulated in this way,which offers a new choice for the design of PN junction based devices.展开更多
During the fabrication of quartz crystal resonators(QCRs),parallelism error is inevitably generated,which is rarely investigated.In order to reveal the influence of parallelism error on the working performance of QCRs...During the fabrication of quartz crystal resonators(QCRs),parallelism error is inevitably generated,which is rarely investigated.In order to reveal the influence of parallelism error on the working performance of QCRs,the coupled vibration of a non-parallel AT-cut quartz crystal plate with electrodes is systematically studied from the views of theoretical analysis and numerical simulations.The two-dimensional thermal incremental field equations are solved for the free vibration analysis via the coefficient-formed partial differential equation module of the COMSOL Multiphysics software,from which the frequency spectra,frequency–temperature curves,and mode shapes are discussed in detail.Additionally,the piezoelectric module is utilized to obtain the admittance response under different conditions.It is demonstrated that the parallelism error reduces the resonant frequency.Additionally,symmetry broken by the non-parallelism increases the probability of activity dip and is harmful to QCR’s thermal stability.However,if the top and bottom surfaces incline synchronously in the same direction,the influence of parallelism error is tiny.The conclusions achieved are helpful for the QCR design,and the methodology presented can also be applied to other wave devices.展开更多
In this paper, we conduct research on the multimedia information retrieval algorithm based on the information restructuring and image reconstruction. With the massive growth of information resources, people through va...In this paper, we conduct research on the multimedia information retrieval algorithm based on the information restructuring and image reconstruction. With the massive growth of information resources, people through various retrieval tools for too much information, led directly to information overload. In vector space model and probability retrieval model based on information retrieval tools rarely consider the user' s personalized information needs and features, has resulted in a large amount of information retrieval result and correlation information the user' s information demand is not big. In order to improve the existing retrieval system, in recent years, scholars to study looked that context information retrieval context factors need to be considered, such as the retrieval time, place and the interactive history, mission, environment and other factors stated or implied in the retrieval process. At present, the context research has become the information behavior, information search process and the research hotspot in the field of information retrieval interaction.展开更多
Root meristem activity is essential for root morphogenesis and adaptation,but the molecular mechanism regulating root meristem activity is not fully understood.Here,we identify an F-box family E3 ubiquitin ligase name...Root meristem activity is essential for root morphogenesis and adaptation,but the molecular mechanism regulating root meristem activity is not fully understood.Here,we identify an F-box family E3 ubiquitin ligase named SHORT PRIMARY ROOT(SHPR) that regulates primary root(PR)meristem activity and cell proliferation in rice.SHPR loss-of-function mutations impair PR elongation in rice.SHPR is involved in the formation of an SCF complex with the Oryza sativa SKP1-like protein OSK1/20.We show that SHPR interacts with Oryza sativa SEUSS-LIKE(OsSLK) in the nucleus and is required for OsSLK polyubiquitination and degradation by the ubiquitin 26S-proteasome system(UPS).Transgenic plants overexpressing OsSLK display a shorter PR phenotype,which is similar to the SHPR loss-of-function mutants.Genetic analysis suggests that SHPR promotes PR elongation in an OsSLK-dependent manner.Collectively,our study establishes SHPR as an E3 ubiquitin ligase that targets OsSLK for degradation,and uncovers a protein ubiquitination pathway as a mechanism for modulating root meristem activity in rice.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.11872329,12192211,and 12072315)the Natural Science Foundation of Zhejiang Province of China(No.LD21A020001)+1 种基金the National Postdoctoral Program for Innovation Talents of China(No.BX2021261)the China Postdoctoral Science Foundation Funded Project(No.2022M722745)。
文摘The dispersion curves of bulk waves propagating in both AlN and ZnO film bulk acoustic resonators(FBARs)are presented to illustrate the mode flip of the thickness-extensional(TE)and 2nd thickness-shear(TSh2)modes.The frequency spectrum quantitative prediction(FSQP)method is used to solve the frequency spectra for predicting the coupling strength among the eigen-modes in AlN and ZnO FBARs.The results elaborate that the flip of the TE and TSh2 branches results in novel self-coupling vibration between the small-wavenumber TE and large-wavenumber TE modes,which has never been observed in the ZnO FBAR.Besides,the mode flip leads to the change in the relative positions of the frequency spectral curves about the TE cut-off frequency.The obtained frequency spectra can be used to predict the mode-coupling behaviors of the vibration modes in the AlN FBAR.The conclusions drawn from the results can help to distinguish the desirable operation modes of the AlN FBAR with very weak coupling strength from all vibration modes.
基金National Natural Science Foundation of China,Grant/Award Number:32070542Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2021A1515010873 and 2022A1515011455+1 种基金Breed Industry Innovation Park of Guangdong Xiaoerhua Pig,Grant/Award Number:2022-4408X1-43010402-0019Hainan Provincial Natural Science Foundation,Grant/Award Number:818MS132。
文摘Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood supply.Previous research has suggested that au-tophagy and angiogenesis play vital roles in the prevention of heart failure after MI,and miR-106a is considered to be an important regulatory factor in MI.But the specific mechanism remains unknown.In this study,using cultured venous endothelial cells and a rat model of MI,we aimed to identify the potential target genes of miR-106a and discover the mechanisms of inhibiting autophagy and angiogenesis.Methods:We first explored the biological functions of miR-106a on autophagy and angiogenesis on endothelial cells.Then we identified ATG7,which was the down-stream target gene of miR-106a.The expression of miR-106a and ATG7 was investi-gated in the rat model of MI.Results:We found that miR-106a inhibits the proliferation,cell cycle,autophagy and angiogenesis,but promoted the apoptosis of vein endothelial cells.Moreover,ATG7 was identified as the target of miR-106a,and ATG7 rescued the inhibition of autophagy and angiogenesis by miR-106a.The expression of miR-106a in the rat model of MI was decreased but the expression of ATG7 was increased in the infarction areas.Conclusion:Our results indicate that miR-106a may inhibit autophagy and angiogenesis by targeting ATG7.This mechanism may be a potential therapeutic treatment for MI.
基金Supported by National Natural Science Foundation of China(31260061,31060039)Project of Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXZD201601)+1 种基金Key Discipline Construction Project of Kunming UniversityNational College Students' Innovation Project of China
文摘[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shock factor AtHsfAl a and programmed cell death in A. thaliana. [ Method ] Different genotypes of A. thaliana (AtHsfAla-silenced transgenic and wild-type) seedlings were treated at 42 ℃. According to the fragmentation level of fluorogenic substrate Ac-DEVD- pNA, Caspase-3 activity was determined by spectrophotometry. [ Result] After high temperature treatment, Caspase-3 activity in A. thaliana was enhanced signifi-cantly. Caspase-3 activity in AtHsfAla-si/enced transgenic A. thaliana was higher than that in wild-type A. thaliana, which indicated that AtHsfAla could inhibit Caspase-3 activity in A. thaliana under high temperature stress. [ Conclusion] Under high temperature stress, heat shock factor AtHsfAla might exert inhibitory effects on programmed cell death by reducing Caspase-3 activity. This study provided the basis for clarifying the mechanism of stress resistance in plants.
基金support by National Science Foundation for Young Scientists of China under Grant[No.11802116].
文摘The investigation focusing on the mechanical behaviors at the microstructural level in composite materials can provide valuable insight into the failure mechanisms at larger scales.A micromechanics damage model which comprises the coupling of the matrix constitutive model and the cohesive zone(CZM)model at fiber-matrix interfaces is presented to evaluate the transverse tensile damage behaviors of unidirectional(UD)fiber-reinforced polymer(FRP)composites.For the polymeric matrix that exhibits highly non-linear mechanical responses,special focus is paid on the formulation of the constitutive model,which characterizes a mixture of elasticity,plasticity as well as damage.The proposed constitutive model includes the numerical implementation of a fracture plane based ellipse-parabola criterion that is an extension of the classic Mohr-Coulomb criterion,corresponding post-yield flow rule and post-failure degradation rule in the fully implicit integration scheme.The numerical results are in good agreement with experimental measurements.It is found that directly using the matrix properties measured at the ply level to characterize the mechanical responses at the constituent level may bring large discrepancies in homogenized stress-strain responses and dominant failure mechanisms.The distribution of fracture plane angles in matrix is predicted,where it is shown to provide novel insight into the microscopic damage initiation and accumulation under transverse tension.
基金Supported by National Natural Science Foundation of China(31260061,31060039)Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXZD201601)+1 种基金Key Discipline Construction Project of Kunming UniversityNational College Students'Innovation Project of China
文摘[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala (SALK-068042) and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology (GO) analysis. Signal transduction pathways, in which differentia|ly expressed genes were involved, were analyzed by pathway analysis. Gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.
基金Supported by National Natural Science Foundation of China(31260061,31060039)Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXZD201601)+1 种基金Key Discipline Construction Project of Kunming UniversityNational College Students Innovation Project of China
文摘[Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on programmed cell death in Arabidopsis thaliana under cold stress. [ Method] AtHsfAla-silenced transgenic (NT) and wild-type (WT) A. thaliana seedlings were used as experimental materials to induce the formation of callus; the callus were cultured to single cells by suspension culture, subjected to cold stress, stained with DAPI, prepared into cell smears and observed under a fluorescence microscope. [ Result] Under cold stress, cell nucleus of wild-type A. thaliana displayed morphological changes, but no apoptotic bodies were found; apoptotic bodies were observed in AtHsfAla-silenced transgenic A. thaliana cells, and the cytoplasm was remarkably concentrated. [ Conclusion] Under cold stress, heat shock factor AtHsfAla exerted inhibitory effects on programmed cell death in A. thaliana, which was of great significance for clarifying the mechanism of stress responses in plants.
基金国家重点研发项目(2022YFA1503200,2021YFC2101901)国家自然科学基金(22122103,21971108,21971111,22271144)+1 种基金中央高校基本科研业务费(020514380304,020514380252 and 020514380272)江苏省研究生科研与实践创新计划项目(KYCX22_0100).
基金supported by the National Natural Science Foundation of China(Nos.12061131013,11972276,1211101401,12172171,and 12102183)the State Key Laboratory of Mechanics and Control of Mechanical Structures of Nanjing University of Aeronautics and Astronautics(No.MCMS-E-0520K02)+5 种基金the Fundamental Research Funds for the Central Universities of China(Nos.NE2020002 and NS2019007)the National Natural Science Foundation of China for Creative Research Groups(No.51921003)the Postgraduate Research&Practice Innovation Program of Jiangsu Province of China(No.KYCX210179)the National Natural Science Foundation of Jiangsu Province of China(No.BK20211176)the Local Science and Technology Development Fund Projects Guided by the Central Government of China(No.2021Szvup061)the Jiangsu High-Level Innovative and Entrepreneurial Talents Introduction Plan(Shuangchuang Doctor Program,No.JSSCBS20210166)。
文摘In this paper,we propose a specific two-layer model consisting of a functionally graded(FG)layer and a piezoelectric semiconductor(PS)layer.Based on the macroscopic theory of PS materials,the effects brought about by the attached FG layer on the piezotronic behaviors of homogeneous n-type PS fibers and PN junctions are investigated.The semi-analytical solutions of the electromechanical fields are obtained by expanding the displacement and carrier concentration variation into power series.Results show that the antisymmetry of the potential and electron concentration distributions in homogeneous n-type PS fibers is destroyed due to the material inhomogeneity of the attached FG layer.In addition,by creating jump discontinuities in the material properties of the FG layer,potential barriers/wells can be produced in the middle of the fiber.Similarly,the potential barrier configuration near the interface of a homogeneous PS PN junction can also be manipulated in this way,which offers a new choice for the design of PN junction based devices.
基金supported by the National Natural Science Foundation of China(12061131013,11972276,12172171 and 12102183)the Fundamental Research Funds for the Central Universities(NE2020002 andNS2022011)+5 种基金JiangsuHigh-Level Innovative and Entrepreneurial Talents Introduction Plan(Shuangchuang Doctor Program,JSSCBS20210166)the National Natural Science Foundation of Jiangsu Province(BK20211176)the State Key Laboratory of Mechanics and Control of Mechanical Structures at NUAA(No.MCMS-I-0522G01)Local Science andTechnologyDevelopment Fund ProjectsGuided by the CentralGovernment(2021Szvup061)the Opening Projects from the Key Laboratory of Impact and Safety Engineering of Ningbo University(CJ202104)a project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘During the fabrication of quartz crystal resonators(QCRs),parallelism error is inevitably generated,which is rarely investigated.In order to reveal the influence of parallelism error on the working performance of QCRs,the coupled vibration of a non-parallel AT-cut quartz crystal plate with electrodes is systematically studied from the views of theoretical analysis and numerical simulations.The two-dimensional thermal incremental field equations are solved for the free vibration analysis via the coefficient-formed partial differential equation module of the COMSOL Multiphysics software,from which the frequency spectra,frequency–temperature curves,and mode shapes are discussed in detail.Additionally,the piezoelectric module is utilized to obtain the admittance response under different conditions.It is demonstrated that the parallelism error reduces the resonant frequency.Additionally,symmetry broken by the non-parallelism increases the probability of activity dip and is harmful to QCR’s thermal stability.However,if the top and bottom surfaces incline synchronously in the same direction,the influence of parallelism error is tiny.The conclusions achieved are helpful for the QCR design,and the methodology presented can also be applied to other wave devices.
文摘In this paper, we conduct research on the multimedia information retrieval algorithm based on the information restructuring and image reconstruction. With the massive growth of information resources, people through various retrieval tools for too much information, led directly to information overload. In vector space model and probability retrieval model based on information retrieval tools rarely consider the user' s personalized information needs and features, has resulted in a large amount of information retrieval result and correlation information the user' s information demand is not big. In order to improve the existing retrieval system, in recent years, scholars to study looked that context information retrieval context factors need to be considered, such as the retrieval time, place and the interactive history, mission, environment and other factors stated or implied in the retrieval process. At present, the context research has become the information behavior, information search process and the research hotspot in the field of information retrieval interaction.
基金supported by grants from the National Natural Science Foundation of China (31671516 and 31970806)。
文摘Root meristem activity is essential for root morphogenesis and adaptation,but the molecular mechanism regulating root meristem activity is not fully understood.Here,we identify an F-box family E3 ubiquitin ligase named SHORT PRIMARY ROOT(SHPR) that regulates primary root(PR)meristem activity and cell proliferation in rice.SHPR loss-of-function mutations impair PR elongation in rice.SHPR is involved in the formation of an SCF complex with the Oryza sativa SKP1-like protein OSK1/20.We show that SHPR interacts with Oryza sativa SEUSS-LIKE(OsSLK) in the nucleus and is required for OsSLK polyubiquitination and degradation by the ubiquitin 26S-proteasome system(UPS).Transgenic plants overexpressing OsSLK display a shorter PR phenotype,which is similar to the SHPR loss-of-function mutants.Genetic analysis suggests that SHPR promotes PR elongation in an OsSLK-dependent manner.Collectively,our study establishes SHPR as an E3 ubiquitin ligase that targets OsSLK for degradation,and uncovers a protein ubiquitination pathway as a mechanism for modulating root meristem activity in rice.