Oligo probe staining is a low-cost and efficient chromosome identification technique.In this study,oligo genomic in situ hybridization(Oligo-GISH)technology was established in peanut.Peanut A and B subgenome-specific ...Oligo probe staining is a low-cost and efficient chromosome identification technique.In this study,oligo genomic in situ hybridization(Oligo-GISH)technology was established in peanut.Peanut A and B subgenome-specific interspersed repeat(IR)oligo probe sets were developed based on clustering and electronic localization of tandem repeat sequences in the reference genome of Tifrunner.The OligoGISH kit was then used to perform staining of 15 Arachis species.The A-subgenome probe set stained the chromosomes of A-and E-genome Arachis species,the B-subgenome probe set stained those of B-,F-,K-,and E-genome species,and neither set stained those of H-genome species.These results indicate the relationships among the genomes of these Arachis species.The Oligo-GISH kit was also used for batch staining of the chromosomes of 389 seedlings from the irradiated M1generation,allowing 67 translocation and deletion lines to be identified.Subsequent Oligo-FISH karyotyping,FISH using single-copy probe libraries,and trait investigation identified seven homozygous chromosomal variants from the M3generation and suggested that there may be genes on chromosome 4B controlling seed number per pod.These findings demonstrate that the IR probe sets and method developed in this study can facilitate research on distant hybridization and genetic improvement in peanut.展开更多
Sea fog is a disastrous weather phenomenon,posing a risk to the safety of maritime transportation.Dense sea fogs reduce visibility at sea and have frequently caused ship collisions.This study used a geographically wei...Sea fog is a disastrous weather phenomenon,posing a risk to the safety of maritime transportation.Dense sea fogs reduce visibility at sea and have frequently caused ship collisions.This study used a geographically weighted regression(GWR)model to explore the spatial non-stationarity of near-miss collision risk,as detected by a vessel conflict ranking operator(VCRO)model from automatic identification system(AIS)data under the influence of sea fog in the Bohai Sea.Sea fog was identified by a machine learning method that was derived from Himawari-8 satellite data.The spatial distributions of near-miss collision risk,sea fog,and the parameters of GWR were mapped.The results showed that sea fog and near-miss collision risk have specific spatial distribution patterns in the Bohai Sea,in which near-miss collision risk in the fog season is significantly higher than that outside the fog season,especially in the northeast(the sea area near Yingkou Port and Bayuquan Port)and the southeast(the sea area near Yantai Port).GWR outputs further indicated a significant correlation between near-miss collision risk and sea fog in fog season,with higher R-squared(0.890 in fog season,2018),than outside the fog season(0.723 in non-fog season,2018).GWR results revealed spatial non-stationarity in the relationships between-near miss collision risk and sea fog and that the significance of these relationships varied locally.Dividing the specific navigation area made it possible to verify that sea fog has a positive impact on near-miss collision risk.展开更多
With continuous mutations of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the severe immune escape of Omicron sub-variants urges the development of next-generation broad-spectrum vaccines,especially as ...With continuous mutations of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the severe immune escape of Omicron sub-variants urges the development of next-generation broad-spectrum vaccines,especially as booster jabs after high-level vaccination coverage of inactivated vaccines in China and many other countries.Previously,we developed a coronavirus disease 2019(COVID-19)protein subunit vaccine ZF2001?based on the tandem homo-prototype receptor-binding domain(RBD)-dimer of the SARS-CoV-2 spike protein.We upgraded the antigen into a hetero-chimeric prototype(PT)-Beta or Delta-BA.1 RBD-dimer to broaden the cross-protection efficacy and prove its efficiency with protein subunit and mRNA vaccine platforms.Herein,we further explored the hetero-chimeric RBD-dimer mRNA vaccines and evaluated their broad-spectrum activities as booster jabs following two doses of inactivated vaccine(Ⅳ)in mice.Our data demonstrated that the chi-meric vaccines significantly boosted neutralizing antibody levels and specific T-cell responses against the vari-ants,and PT-Beta was superior to Delta-BA.1 RBD as a booster in mice,shedding light on the antigen design for the next-generation COVID-19 vaccines.展开更多
The coronavirus disease 2019(COVID-19)pandemic has ravaged theworld for nearly 2 years,with continuous emergence of new variants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Omicron,the latest Varian...The coronavirus disease 2019(COVID-19)pandemic has ravaged theworld for nearly 2 years,with continuous emergence of new variants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Omicron,the latest Variant of Concern(VOC),has spread to all six continents,leading to a rapid increase in confirmed COVID-19 cases and surpassing Delta as the dominant variant in many countries.展开更多
To the Editor： Percutaneous endoscopic lumbar discectomy （PELD） becomes increasingly popular among spine surgeries in recent years. PELD is one of the minimally invasive spine surgeries with significant advantages ...To the Editor： Percutaneous endoscopic lumbar discectomy （PELD） becomes increasingly popular among spine surgeries in recent years. PELD is one of the minimally invasive spine surgeries with significant advantages including small incision, less muscle stripping, and enhanced postoperative recovery.展开更多
ABSTRACT Introduction:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a recently emergent coronavirus of natural origin and caused the coronavirus disease(COVID-19)pandemic.The study of its natural origi...ABSTRACT Introduction:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a recently emergent coronavirus of natural origin and caused the coronavirus disease(COVID-19)pandemic.The study of its natural origin and host range is of particular importance for source tracing,monitoring of this virus,and prevention of recurrent infections.One major approach is to test the binding ability of the viral receptor gene ACE2 from various hosts to SARS-CoV-2 spike protein,but it is time-consuming and laborintensive to cover a large collection of species.展开更多
基金supported by National Natural Science Foundation of China(31801397)Fund for Distinguished Young Scholars of Henan Academy of Agricultural Sciences(2020JQ03)+3 种基金Independent Innovation Foundation of Henan Academy of Agricultural Sciences,China(2022ZC69)China Agriculture Research System(CARS-13)Key Scientific and Technological Project of Henan Province(201300111000)Henan Provincial Agriculture Research System(S2012-5)。
文摘Oligo probe staining is a low-cost and efficient chromosome identification technique.In this study,oligo genomic in situ hybridization(Oligo-GISH)technology was established in peanut.Peanut A and B subgenome-specific interspersed repeat(IR)oligo probe sets were developed based on clustering and electronic localization of tandem repeat sequences in the reference genome of Tifrunner.The OligoGISH kit was then used to perform staining of 15 Arachis species.The A-subgenome probe set stained the chromosomes of A-and E-genome Arachis species,the B-subgenome probe set stained those of B-,F-,K-,and E-genome species,and neither set stained those of H-genome species.These results indicate the relationships among the genomes of these Arachis species.The Oligo-GISH kit was also used for batch staining of the chromosomes of 389 seedlings from the irradiated M1generation,allowing 67 translocation and deletion lines to be identified.Subsequent Oligo-FISH karyotyping,FISH using single-copy probe libraries,and trait investigation identified seven homozygous chromosomal variants from the M3generation and suggested that there may be genes on chromosome 4B controlling seed number per pod.These findings demonstrate that the IR probe sets and method developed in this study can facilitate research on distant hybridization and genetic improvement in peanut.
文摘Sea fog is a disastrous weather phenomenon,posing a risk to the safety of maritime transportation.Dense sea fogs reduce visibility at sea and have frequently caused ship collisions.This study used a geographically weighted regression(GWR)model to explore the spatial non-stationarity of near-miss collision risk,as detected by a vessel conflict ranking operator(VCRO)model from automatic identification system(AIS)data under the influence of sea fog in the Bohai Sea.Sea fog was identified by a machine learning method that was derived from Himawari-8 satellite data.The spatial distributions of near-miss collision risk,sea fog,and the parameters of GWR were mapped.The results showed that sea fog and near-miss collision risk have specific spatial distribution patterns in the Bohai Sea,in which near-miss collision risk in the fog season is significantly higher than that outside the fog season,especially in the northeast(the sea area near Yingkou Port and Bayuquan Port)and the southeast(the sea area near Yantai Port).GWR outputs further indicated a significant correlation between near-miss collision risk and sea fog in fog season,with higher R-squared(0.890 in fog season,2018),than outside the fog season(0.723 in non-fog season,2018).GWR results revealed spatial non-stationarity in the relationships between-near miss collision risk and sea fog and that the significance of these relationships varied locally.Dividing the specific navigation area made it possible to verify that sea fog has a positive impact on near-miss collision risk.
基金supported by the National Key R&D Program of China(2022YFC2303403)the National Natural Science Foundation of China(82225021 and 32171428)the CAS Young Scientists in Basic Research(YSBR-010)。
基金This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(grant number XDB29040203)the National Key Research and Development Program of China(grant number 2021YFA1301404 and 2020YFA0907102)+2 种基金the National Natural Science Foundation of China(grant numbers 82225021 and 32171428)In addition,Qihui Wang was supported by the CAS Project for Young Scientists in Basic Research(grant number YSBR-010)the Youth Innovation Promotion Association of the CAS(grant number Y2022037).We thank Professor Xiao Zhao from the National Center for Nanoscience and Technology for sharing the LNP encapsulation and DLS platforms.We thank Dr.Kun Xu for his help during the revision of this manuscript.We thank Linjie Li for sharing recombinant RBD proteins.We thank the Institutional Center for Shared Technology and Facilitates in the Institute of Microbiology,CAS,and the Institute of Zoology,CAS.
文摘With continuous mutations of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the severe immune escape of Omicron sub-variants urges the development of next-generation broad-spectrum vaccines,especially as booster jabs after high-level vaccination coverage of inactivated vaccines in China and many other countries.Previously,we developed a coronavirus disease 2019(COVID-19)protein subunit vaccine ZF2001?based on the tandem homo-prototype receptor-binding domain(RBD)-dimer of the SARS-CoV-2 spike protein.We upgraded the antigen into a hetero-chimeric prototype(PT)-Beta or Delta-BA.1 RBD-dimer to broaden the cross-protection efficacy and prove its efficiency with protein subunit and mRNA vaccine platforms.Herein,we further explored the hetero-chimeric RBD-dimer mRNA vaccines and evaluated their broad-spectrum activities as booster jabs following two doses of inactivated vaccine(Ⅳ)in mice.Our data demonstrated that the chi-meric vaccines significantly boosted neutralizing antibody levels and specific T-cell responses against the vari-ants,and PT-Beta was superior to Delta-BA.1 RBD as a booster in mice,shedding light on the antigen design for the next-generation COVID-19 vaccines.
文摘The coronavirus disease 2019(COVID-19)pandemic has ravaged theworld for nearly 2 years,with continuous emergence of new variants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Omicron,the latest Variant of Concern(VOC),has spread to all six continents,leading to a rapid increase in confirmed COVID-19 cases and surpassing Delta as the dominant variant in many countries.
文摘To the Editor： Percutaneous endoscopic lumbar discectomy （PELD） becomes increasingly popular among spine surgeries in recent years. PELD is one of the minimally invasive spine surgeries with significant advantages including small incision, less muscle stripping, and enhanced postoperative recovery.
基金The Strategic Priority Research Programs of the Chinese Academy of Sciences(XDB29020000)the National Natural Science Foundation of China(32041009)Key R&D Program of Shandong Province(2020CXGC011305).
文摘ABSTRACT Introduction:Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a recently emergent coronavirus of natural origin and caused the coronavirus disease(COVID-19)pandemic.The study of its natural origin and host range is of particular importance for source tracing,monitoring of this virus,and prevention of recurrent infections.One major approach is to test the binding ability of the viral receptor gene ACE2 from various hosts to SARS-CoV-2 spike protein,but it is time-consuming and laborintensive to cover a large collection of species.
