Oxidation and reduction of some amino acids are one of the molecular mechanisms for regulating the function of proteins. The oxidation of methionine (Met) to methionine sulfoxide (Met(O)) results in decreasing or loss...Oxidation and reduction of some amino acids are one of the molecular mechanisms for regulating the function of proteins. The oxidation of methionine (Met) to methionine sulfoxide (Met(O)) results in decreasing or loss of the biological activity of related proteins. It was found that peptide methionine sulfoxide reductase (msrA) can reduce Met(O) to Met and therefore restored the biological function of the oxidized proteins. To reveal the methionine oxidation-reduction mechanism in human body, in this study, the cDNA sequence of bovine msrA was used as an information-probe to screen the human EST database. Based on a contig assembled from homologous ESTs, a 1 256-bp human MSRA cDNA was cloned from several human cDNA libraries. The cDNA contains an open reading frame (ORF) of 705 bp in length, which encodes 235 amino acid residues. Homology comparison revealed that human MSRA shares 88% and 61% identities with bovine and Escherichia coli msrA protein respectively. Expression pattern analysis revealed a展开更多
A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library. A nucleotide sequence of 1 225 bp length has been determined containing an open reading frame of...A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library. A nucleotide sequence of 1 225 bp length has been determined containing an open reading frame of 1 044 nucleotides (encoding 348 amino acids). In view of its degree of homology to members of the Ser/Thr protein kinase family and the closest relationship to Mus musculus STK-1, the predicted product was designated by the name of HUMSTK-1. Its mRNA is present in large amounts in thymus, and small amounts in testis,small intestine and colon.展开更多
An EST （gb/AA115239） with high identity to the mouse cytokine signal transduction inhibitor gene mmSOCS-2 was selected in GenBank EST database by the homologous screening method. The cDNA with the same sequence of t...An EST （gb/AA115239） with high identity to the mouse cytokine signal transduction inhibitor gene mmSOCS-2 was selected in GenBank EST database by the homologous screening method. The cDNA with the same sequence of the EST was got in human placenta cDNA library by PCR and a 1011 bp cDNA fragment was selected using above cDNA as probes to perform walking hybridization in placenta cDNA library. The cDNA fragment contains one 594 bp open reading frame （ORF） which encodes 198 amino acid residues. It was proved to be novel after NCBI database screening. Homology comparison showed that this gene has 93% identity to mmSOCS-2 at the amino acid level and it has high identities to other related genes in SH<sub>2</sub> domain and SOCS box, so it was named humSOCS-2 and the accession number in GenBank is gb/AF020590. The expression analysis showed that the gene is expressed obviously higher in prostate than in other 15 human tissues.展开更多
ZINC finger proteins play an important role in the regulation of gene expression by binding to DNA/RNA in a sequence-specific manner. Now nearly 200 human zinc finger genes have been cloned. According to the differenc...ZINC finger proteins play an important role in the regulation of gene expression by binding to DNA/RNA in a sequence-specific manner. Now nearly 200 human zinc finger genes have been cloned. According to the difference of the conservative domain, the zinc finger proteins can be classified into several types.Most of zinc finger proteins belong to the C<sub>2</sub>H<sub>2</sub> type containing the consensus amino acid sequence YECX<sub>2</sub>CX<sub>3</sub>FX<sub>5</sub>LX<sub>2</sub>HX<sub>3</sub>HTGEKP, which is rather conservative especially in the link region between two zinc finger motifs （TGEKP）展开更多
文摘Oxidation and reduction of some amino acids are one of the molecular mechanisms for regulating the function of proteins. The oxidation of methionine (Met) to methionine sulfoxide (Met(O)) results in decreasing or loss of the biological activity of related proteins. It was found that peptide methionine sulfoxide reductase (msrA) can reduce Met(O) to Met and therefore restored the biological function of the oxidized proteins. To reveal the methionine oxidation-reduction mechanism in human body, in this study, the cDNA sequence of bovine msrA was used as an information-probe to screen the human EST database. Based on a contig assembled from homologous ESTs, a 1 256-bp human MSRA cDNA was cloned from several human cDNA libraries. The cDNA contains an open reading frame (ORF) of 705 bp in length, which encodes 235 amino acid residues. Homology comparison revealed that human MSRA shares 88% and 61% identities with bovine and Escherichia coli msrA protein respectively. Expression pattern analysis revealed a
文摘A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library. A nucleotide sequence of 1 225 bp length has been determined containing an open reading frame of 1 044 nucleotides (encoding 348 amino acids). In view of its degree of homology to members of the Ser/Thr protein kinase family and the closest relationship to Mus musculus STK-1, the predicted product was designated by the name of HUMSTK-1. Its mRNA is present in large amounts in thymus, and small amounts in testis,small intestine and colon.
文摘An EST （gb/AA115239） with high identity to the mouse cytokine signal transduction inhibitor gene mmSOCS-2 was selected in GenBank EST database by the homologous screening method. The cDNA with the same sequence of the EST was got in human placenta cDNA library by PCR and a 1011 bp cDNA fragment was selected using above cDNA as probes to perform walking hybridization in placenta cDNA library. The cDNA fragment contains one 594 bp open reading frame （ORF） which encodes 198 amino acid residues. It was proved to be novel after NCBI database screening. Homology comparison showed that this gene has 93% identity to mmSOCS-2 at the amino acid level and it has high identities to other related genes in SH<sub>2</sub> domain and SOCS box, so it was named humSOCS-2 and the accession number in GenBank is gb/AF020590. The expression analysis showed that the gene is expressed obviously higher in prostate than in other 15 human tissues.
文摘ZINC finger proteins play an important role in the regulation of gene expression by binding to DNA/RNA in a sequence-specific manner. Now nearly 200 human zinc finger genes have been cloned. According to the difference of the conservative domain, the zinc finger proteins can be classified into several types.Most of zinc finger proteins belong to the C<sub>2</sub>H<sub>2</sub> type containing the consensus amino acid sequence YECX<sub>2</sub>CX<sub>3</sub>FX<sub>5</sub>LX<sub>2</sub>HX<sub>3</sub>HTGEKP, which is rather conservative especially in the link region between two zinc finger motifs （TGEKP）
