The endometrium is composed of glandular and luminal epithelia supported by stromal connective tissue and multiple other cell types.It is a dynamic organ that undergoes physiological and functional alteration during t...The endometrium is composed of glandular and luminal epithelia supported by stromal connective tissue and multiple other cell types.It is a dynamic organ that undergoes physiological and functional alteration during the menstrual cycle.Organoids resemble the primary tissue of origin to recapitulate their corresponding biological and pathological characteristics.They are known for their ability to undergo extensive expansion while maintaining their genomic stability,facilitating their long-term storage and high-throughput screening.The development of the three-dimensional endometrial organoid system,which recapitulates the structural and functional characteristics of the endometrial glands,provides a powerful tool to study the normal endometrium and its related diseases.The Web of Science was searched for relevant literature using the keywords"endometrium","endometrial gland","organoid",and"culture model";a total of 134 articles were selected.In this review,the characteristics,applications,and limitations of endometrial epithelial organoids are discussed.展开更多
The human endometrium and embryo both produce microRNAs(miRNAs),which are involved in various physiological activities.Accumulating evident suggested that endometrial miRNAs are regulated by steroid hormones that modu...The human endometrium and embryo both produce microRNAs(miRNAs),which are involved in various physiological activities.Accumulating evident suggested that endometrial miRNAs are regulated by steroid hormones that modulate endometrial functions,whereas embryo-derived miRNAs play vital roles in maternal–embryo communication.The aberrant expression of endometriumor embryo-derived miRNAs can cause early pregnancy and gestational disorders,including repeated implantation failure,recurrent miscarriage,and pathological conditions such as endometriosis.miRNAs are,therefore,ideal candidates for conducting clinicopathological tests.Potential diagnostic and prognostic tests using miRNAs are under development;however,the therapeutic applications of miRNAs in regulating gestational disorders require further investigation.展开更多
基金supported in part by the National Natural Science Foundation of China(81971396)Hong Kong Research Grant Council Grant(17115619&17115320)+1 种基金High Level-Hospital Program,Health Commission of Guangdong Province,China(HKUSZH201902015)HKU-SZH Fund for Shenzhen Key Medical Discipline(SZXK2020089)
文摘The endometrium is composed of glandular and luminal epithelia supported by stromal connective tissue and multiple other cell types.It is a dynamic organ that undergoes physiological and functional alteration during the menstrual cycle.Organoids resemble the primary tissue of origin to recapitulate their corresponding biological and pathological characteristics.They are known for their ability to undergo extensive expansion while maintaining their genomic stability,facilitating their long-term storage and high-throughput screening.The development of the three-dimensional endometrial organoid system,which recapitulates the structural and functional characteristics of the endometrial glands,provides a powerful tool to study the normal endometrium and its related diseases.The Web of Science was searched for relevant literature using the keywords"endometrium","endometrial gland","organoid",and"culture model";a total of 134 articles were selected.In this review,the characteristics,applications,and limitations of endometrial epithelial organoids are discussed.
基金the RGC General Research Fund(17120720)Health and Medical Research Fund,Hong Kong(08192326)Conference and Research Committee grant from the University of Hong Kong to K.F.L.
文摘The human endometrium and embryo both produce microRNAs(miRNAs),which are involved in various physiological activities.Accumulating evident suggested that endometrial miRNAs are regulated by steroid hormones that modulate endometrial functions,whereas embryo-derived miRNAs play vital roles in maternal–embryo communication.The aberrant expression of endometriumor embryo-derived miRNAs can cause early pregnancy and gestational disorders,including repeated implantation failure,recurrent miscarriage,and pathological conditions such as endometriosis.miRNAs are,therefore,ideal candidates for conducting clinicopathological tests.Potential diagnostic and prognostic tests using miRNAs are under development;however,the therapeutic applications of miRNAs in regulating gestational disorders require further investigation.
