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公交投币机纸币模块结构与控制系统设计 被引量:1
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作者 黄嘉兴 黄希 +1 位作者 戚义鹏 朱云鹏 《机械设计与制造》 北大核心 2022年第7期19-23,28,共6页
针对目前我国公交投币机功能单一问题,设计了一种可用于公交投币机的新型纸币处理模块。该模块由检测单元、传输单元、清分存储单元、找零单元和控制系统组成。为适应公交投币机的实际使用中快速性要求和降低找零时对纸币的质量要求,提... 针对目前我国公交投币机功能单一问题,设计了一种可用于公交投币机的新型纸币处理模块。该模块由检测单元、传输单元、清分存储单元、找零单元和控制系统组成。为适应公交投币机的实际使用中快速性要求和降低找零时对纸币的质量要求,提出了纸币快速处理方案,设计了可利用卷膜将纸币卷存于滚筒表面的机械结构。采用Arduino Mega2560单片机作为样机控制系统的处理器,并利用MATLAB软件对步进电机控制系统的快速性、可行性进行了模拟验证。该模块设计可以实现纸币的自动识别清分存储与快速循环找零功能。 展开更多
关键词 公交投币机 纸币处理 ARDUINO 循环找零 清分计数
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Utilization of Alternative Polyadenylation Signals in the Novel Human Apoptosis-Inducing Gene hap
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作者 Qu Xiao-ling qi yi-peng qi Bing 《Wuhan University Journal of Natural Sciences》 EI CAS 2001年第4期867-872,共6页
hap, a novel human apoptosis-inducing gene which can interact with another newly discovered apoptosis-inducing geneASY, was identified, by cloning its cDNAs from human lung cell line (WI-38) cDNA library. Two major mR... hap, a novel human apoptosis-inducing gene which can interact with another newly discovered apoptosis-inducing geneASY, was identified, by cloning its cDNAs from human lung cell line (WI-38) cDNA library. Two major mRNA species (1.8 and 2.7 kb in length, respectively) were previously identified by Northern blot analysis of poly(A)+ RNA from human multiple tissues using partialhap cDNA as a probe. In the present work, the molecular mechanism accounting for the generation of the twohap transcripts were investigated. The rapid amplification of cDNA 3′-ends (3′-RACE) technique and the sequential Southern blot analysis, in conjunction with the sequencing analysis demonstrated that the twohap transcripts derive from the alternative polyadenylation site selection: a AATAAA signal at position 1 528–1 533 nt for the 1.8 kbhap mRNA: and a AATAAA signal at position 2 375–2 380 nt for the 2.7 kbhap mRNA. Furthermore, a number of regulatory elements withinhap 3′-untranslated region (3′-UTR) were also examined. 展开更多
关键词 apoptosis-inducing genehap polyadenylation signal regulatory element 3′ untranslated region (3′-UTR)
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Isolation and Characterization of a Novel Gene Regulating Human Cell Death
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作者 qi Bing qi yi-peng 《Wuhan University Journal of Natural Sciences》 CAS 1999年第4期502-502,共1页
A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of... A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase I phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu) is present at the C-terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co-immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos-2 and induce cell apoptosis, but the efficiency is lower than that of asy gene. 展开更多
关键词 asy gene asyip gene APOPTOSIS yeast two hybrid system
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HSVTK Gene Therapy for CarcinoembryonicAntigen-Producing Human Lung Cancer Cells
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作者 Xiao Geng-fu qi yi-peng +1 位作者 Cheng Xuan-hong Li Ling-yun 《Wuhan University Journal of Natural Sciences》 CAS 1999年第3期367-371,共5页
The long-term success of gene therapy for cancer relies heavily on the development of effective targeting systems. We investigate the possibility of targeted gene therapy using promoter of carcinoembryonic antigen (CE... The long-term success of gene therapy for cancer relies heavily on the development of effective targeting systems. We investigate the possibility of targeted gene therapy using promoter of carcinoembryonic antigen (CEA) gene. By using luciferase reporter gene, we found that CEA promoter exhibit 16 times high activity in CEA-producing lung cancer cells, A549 than in nonproducing cells, Hela. We also constructed a recombinant expression plasmid pCEATK, in which CEA promoter drives the effector gene, thymidine kinase gene of Herpes Simplex Virus (HSVTK). A549 cells transfected with pCEATK became 865 times more sensitive to ganciclovir (GCV) than the control cells. However, Hela cells transfected with this plasmid remained resistant to GCV. These data indicate the potential for targeted gene therapy using the CEA promoter against CEA-producing tumor cells, such as lung cancer cells. 展开更多
关键词 carcinoembryonic antigen Herpes Simplex Virus thymidine kinase gene gene therapy
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Studies on the Sructure, Mechanism of Green Fluorescent Protein and Its Application
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作者 Liu Zu-qiang Hu Min qi yi-peng 《Wuhan University Journal of Natural Sciences》 CAS 2000年第2期252-252,共1页
The green fluorescent protein (GFP) from jellyfishAequorea victoria has unique superiority as a kind of biological label. GFP has been widely used in all fields of biology based on the recent studies on its structure,... The green fluorescent protein (GFP) from jellyfishAequorea victoria has unique superiority as a kind of biological label. GFP has been widely used in all fields of biology based on the recent studies on its structure, characteristic and mechanism of bioluninescence. 展开更多
关键词 green fluorescent protein biological label APPLICATION
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Sequencing of p35 Gene from Heliothis armigera Polyhedrosis Virus and Its Homologous Comparison
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作者 Wang Ye-fu qi yi-peng +2 位作者 Li Zhi-da Zhu Ying Su fei 《Wuhan University Journal of Natural Sciences》 CAS 1999年第2期123-129,共7页
Using p35 gene primers of AcNPV, about 1 kb fragment was obtained by PCR from HaNPV DNA and was sequenced thereafter. It has a full reading frame encode 299 amino acids. Sharing identity of 94% in nucleotides and 84%... Using p35 gene primers of AcNPV, about 1 kb fragment was obtained by PCR from HaNPV DNA and was sequenced thereafter. It has a full reading frame encode 299 amino acids. Sharing identity of 94% in nucleotides and 84% in predict amino acids with AcNPV, a apoptosis inhibiting gene was found. According to comparison of p35 genes in five kinds of baculovirus, it is found that AcNPV, TnNPV and BmNPV share the most intimate blood relation. HaNPV is near the above three species. LsNPV is little remote from them. This result reconfirmed those we have done with gp37 genes and vp39 genes. It is more accurate to use conserved gene for species division than that of the serological identification. 展开更多
关键词 molecular evolution homologous comparison PCR amplification
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