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Effects of nuclear factor-kappaB on rat hepatocyte regeneration and apoptosis after 70% portal branch ligation 被引量:7
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作者 Wen-Jun Yang qi-yu zhang +6 位作者 Zheng-Ping Yu Qi-Tong Song Hua-Ping Liang Xiang Xu Guan-Bao Zhu Fei-Zhao Jiang Hong-Qi Shi 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第43期6775-6779,共5页
AIM: To detect the DNA binding activity of nuclear factor-kappaB (NF-KB) in rat hepatocyte and to investigate the effects of NF-KB on rat hepatocyte regeneration and apoptosis after 70% portal branch Iigation. METH... AIM: To detect the DNA binding activity of nuclear factor-kappaB (NF-KB) in rat hepatocyte and to investigate the effects of NF-KB on rat hepatocyte regeneration and apoptosis after 70% portal branch Iigation. METHODS: Sixty Wistar rats were randomly divided into control group and portal branch ligation group. The animals were killed 12 h, 1, 2, 3, 7, and 14 d after surgery to determine the contents of plasma ALT. Hepatocytes were isolated and nuclear protein was extracted. DNA binding activity of NF-KB was measured by ENSA. Hepatocyte regeneration and apoptosis were observed under microscope by TUNEL staining. The ultrastructural changes of liver were observed under electron microscope. RESULTS: Seventy percent portal branch ligation produced atrophy of the ligated lobes and the perfused lobes underwent compensatory regeneration, the total liver weight and plasma ALT levels were maintained at the level of sham-operated animals throughout the experiment. After 2 d of portal branch ligation, DNA binding activity of NF-KB in hepatocyte increased and reached its peak, the number of apoptotic hepatocyte in the ligated lobes and the number of mitotic hepatocyte in the perfused lobes also reached their peak. Typical apoptotic changes and evident fibrotic changes in the ligated lobes were observed under electron microscope. CONCLUSION: After 70% portal branch ligation, DNA binding activity of NF-KB in hepatocyte is significantly increased and NF-KB plays an important role in hepatocyte regeneration and apoptosis. 展开更多
关键词 Portal branch ligation Nuclear factor-kappaB REGENERATION APOPTOSIS
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Antimicrobial peptides: mechanism of action, activity and clinical potential 被引量:3
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作者 qi-yu zhang Zhi-Bin Yan +7 位作者 Yue-Ming Meng Xiang-Yu Hong Gang Shao Jun-Jie Ma Xu-Rui Cheng Jun Liu Jian Kang Cai-Yun Fu 《Military Medical Research》 SCIE CSCD 2022年第2期231-258,共28页
The management of bacterial infections is becoming a major clinical challenge due to the rapid evolution of antibiotic resistant bacteria.As an excellent candidate to overcome antibiotic resistance,antimicrobial pepti... The management of bacterial infections is becoming a major clinical challenge due to the rapid evolution of antibiotic resistant bacteria.As an excellent candidate to overcome antibiotic resistance,antimicrobial peptides(AMPs)that are produced from the synthetic and natural sources demonstrate a broad-spectrum antimicrobial activity with the high specificity and low toxicity.These peptides possess distinctive structures and functions by employing sophisticated mechanisms of action.This comprehensive review provides a broad overview of AMPs from the origin,structural characteristics,mechanisms of action,biological activities to clinical applications.We finally discuss the strategies to optimize and develop AMP-based treatment as the potential antimicrobial and anticancer therapeutics. 展开更多
关键词 Antimicrobial peptides Antimicrobial resistance Mechanism of action Biological activity Clinical application
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Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6 被引量:3
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作者 Sheng-Chu zhang Yi-Hu Zheng +5 位作者 Pan-Pan Yu Tan Hooi Min Fu-Xiang Yu Chao Ye Yuan-Kang Xie qi-yu zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第28期3696-3704,共9页
AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . MET... AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) signaling pathway with a short hairpin RNA (shRNA) in hepatic stellate cells (HSCs) in vitro . METHODS: HSCs were derived from the livers of adul male Sprague-Dawley rats. IL-6 expression was evalu ated using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. The phosphorylation activity of p38 mitogen activated pro tein kinases (MAPK) and extracellular regulated pro tein kinases (ERK) 1/2 upon induction by IL-17A and suppression by IL-17RA shRNA were examined using Western blotting.RESULTS: IL-6 expression induced by IL-17A was significantly increased compared to control in HSCs (P < 0.01 in a dose-dependent manner). Suppression of IL17RA using lentiviral-mediated shRNA inhibited IL-6 expression induced by IL-17A compared to group with only IL-17A treatment (1.44 ± 0.17 vs 4.07 ± 0.43, P < 0.01). IL-17A induced rapid phosphorylation of p38 MAPK and ERK1/2 after 5 min exposure, and showed the strongest levels of phosphorylation of p38 MAPK and ERK1/2 at 15 min in IL-17A-treated HSCs. IL-6 mRNA expression induced by IL-17A (100 ng/mL) for 3 h exposure was inhibited by preincubation with specific inhibitors of p38 MAPK (SB-203580) and ERK1/2 (PD-98059) compared to groups without inhibitors preincubation (1.67 ± 0.24, 2.01 ± 0.10 vs 4.08 ± 0.59, P < 0.01). Moreover, lentiviral-mediated IL-17RA shRNA 1 inhibited IL-17A-induced IL-6 mRNA expression compared to random shRNA in HSCs (1.44 ± 0.17 vs 3.98 ± 0.68, P < 0.01). Lentiviral-mediated IL17RA shRNA 1 inhibited phosphorylation of p38 MAPK and ERK1/2 induced by 15 min IL-17A (100 ng/mL) exposure. CONCLUSION: Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs. Suppression of IL-17RA expression may be a strategy to reduce the inflammatory response induced by IL-17A in the liver. 展开更多
关键词 Interleukin 17A Interleukin 6 Hepatic stellate cells Liver fibrosis
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Diagnosis and Treatment of Cholangiocarcinoma: A Consensus from Surgical Specialists of China 被引量:2
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作者 Jian-qiang Cai Shou-wang Cai +50 位作者 Wen-ming Cong Min-shan Chen Ping Chen Xiao-ping Chen Yan-ling Chen Yi-fa Chen Chao-liu Dai Qiang Huang Zhi-yong Huang Bo Jiang Bin Jiang Ke-wei Jiang Bo Li Zong-fangLi Li-jian Liang Bin Liu Hui-chun Liu Lian-xin Liu Qing-guang Liu Rong Liu Ying-bin Liu Jian-guo Lu Shi-chun Lu Yi Lu Yi-lei Mao Bin Mei Jun Niu Bao-gang Peng Xiao Qin Yu-dong Qiu Guang-yi Wang Yao-don Wangg Zhi-ming Wang Ren-hua Wan Ya-fu Wu Bao-cai Xing Feng Xia Ge-liang Xu Jia-mei Yang Xiao-fang Yu Yong Zeng Yong-yi Zeng Bi-xiang zhang Bin-hao zhang qi-yu zhang Shui-jun zhang Wan-guang zhang Yong-jie zhang Zhi-wei zhang Dong Zhou Wei-ping Zhou 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2014年第4期469-475,共7页
Cholangiocarcinoma refers to malignant tumors that develop in epithelial lining of biliary system, and it is divided into two categories according to tumor location, intrahepatic cholangiocarcinoma (ICC) and extrahe... Cholangiocarcinoma refers to malignant tumors that develop in epithelial lining of biliary system, and it is divided into two categories according to tumor location, intrahepatic cholangiocarcinoma (ICC) and extrahepatic cholangiocarcinoma (ECC). ICC occurs from the epithelial cells of the intrahepatic bile duct, its branches and interlobular biliary tree; and ECC is divided into hilar cholangiocarcinoma and distal cholangiocarcinoma by the circumscription at the confluence of cystic duct and the common hepatic duct. 展开更多
关键词 A Consensus from Surgical Specialists of China Diagnosis and Treatment of Cholangiocarcinoma
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Heterotopic pancreas: report of 7 patients 被引量:1
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作者 Hong-Qi Shi qi-yu zhang +1 位作者 Hong-Lin Teng Ji-Cai Chen From the Department of General Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2002年第2期299-301,共3页
Objective: To discuss the clinical features of patients with heterotopic pancreas, their diagnosis and surgi- cal treatment. Methods: Seven patients with heterotopic pancreas were treated surgically in our hospital fr... Objective: To discuss the clinical features of patients with heterotopic pancreas, their diagnosis and surgi- cal treatment. Methods: Seven patients with heterotopic pancreas were treated surgically in our hospital from August 1992 to March 1999. Results: Of the 7 patients, 4 had heterotopic pancre- as located in the duodenum, 2 in the jejunum, and 1 in the stomach. Four patients experienced abdominal pain, 3 icterus, 1 duodenal obstruction, and 1 diges- tive tract bleeding. Three patients were complicated by cholelithiasis, and 1 patient was complicated by diverticulum of the jejunum. All seven patients were misdiagnosed or undefined preoperatively. They un- derwent surgery and were confirmed pathologically. Conclusions: Heterotopic pancreas is extremely diffi- cult to diagnose before operation since no specific clinical signs are seen in such patients. Once diag- nosed with symptoms or not, the patients should un- dergo surgery for correct diagnosis and avoidance of relative complications. 展开更多
关键词 heterotopic pancreas clinical manifestation DIAGNOSIS THERAPY
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Inhibition of pancreatic stellate cell activity by adipose-derived stem cells 被引量:1
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作者 Fu-Xiang Yu Long-Feng Su +5 位作者 Chun-Lei Dai Yang Wang Yin-Yan Teng Jun-Hui Fu qi-yu zhang Yin-He Tang 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2015年第2期215-221,共7页
BACKGROUND:Pancreatic stellate cells(PSCs)play a critical role in the development of pancreatic fibrosis.In this study we used a novel method to isolate and culture rat PSCs and then investigated the inhibitory eff... BACKGROUND:Pancreatic stellate cells(PSCs)play a critical role in the development of pancreatic fibrosis.In this study we used a novel method to isolate and culture rat PSCs and then investigated the inhibitory effects of adipose-derived stem cells(ADSCs)on activation and proliferation of PSCs.METHODS:Pancreatic tissue was obtained from SpragueDawley rats for PSCs isolation.Transwell cell cultures were adopted for co-culture of ADSCs and PSCs. PSCs prolifera- tion and apoptosis were determined using CCK-8 and flow cytometry, respectively, a-SMA expressions were analyzed using Western blotting. The levels of cytokines [nerve growth factor (NGF), interleukin-10 (IL-10) and transforming growth factor-ill (TGF-[31)] in conditioned medium were detected by ELISA. Gene expression (MMP-2, MMP-9 and TIMP-1) was analyzed using qRT-PCR. RESULTS: This method produced 17.6_+6.5 ~ 103 ceils per gram of the body weight with a purity of 90%-95% and a viability of 92%-97%. Co-culture of PSCs with ADSCs significantly inhib- ited PSCs proliferation and induced PSCs apoptosis. Moreover, a-SMA expression was significantly reduced in PSCs+ADSCs compared with that in PSC-only cultures, while expression of fibrinolytic proteins (e.g., MMP-2 and MMP-9) was upregulated and anti-fibrinolytic protein (TIMP-1) was downregulated. In addition, NGF expression was up-regulated, but IL-10 and TGF-β1 expressions were down-regulated in the coculture conditioned medium compared with those in the PSC- only culture medium. CONCLUSIONS: This study provided an easy and reliable technique to isolate PSCs. The data demonstrated the inhibitory effects of ADSCs on the activation and proliferation of PSCs in vitro. 展开更多
关键词 pancreatic stellate cells isolation mesenchymal stem cells pancreatic fibrosis fibrinolytic protein
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Tackling global health security by building an academic community for One Health action 被引量:1
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作者 Xiao-Xi zhang Xin-Chen Li +23 位作者 qi-yu zhang Jing-Shu Liu Le-Fei Han Zohar Lederman Janna M.Schurer Patrícia Poeta Md.Tanvir Rahman Shi-Zhu Li Kokouvi Kassegne Kun Yin Yong-zhang Zhu Shang Xia Lu He Qin-Qin Hu Le-Shan Xiu Jing-Bo Xue Han-Qing Zhao Xi-Han Wangv Logan Wu Xiao-Kui Guo Zhao-Jun Wang Bernhard Schwartländer Ming-Hui Ren Xiao-Nong Zhou 《Infectious Diseases of Poverty》 SCIE CAS CSCD 2023年第4期93-98,共6页
Background One Health approach is crucial to tackling complex global public health threats at the interface of humans, animals, and the environment. As outlined in the One Health Joint Plan of Action, the internationa... Background One Health approach is crucial to tackling complex global public health threats at the interface of humans, animals, and the environment. As outlined in the One Health Joint Plan of Action, the international One Health community includes stakeholders from different sectors. Supported by the Bill & Melinda Gates Foundation, an academic community for One Health action has been proposed with the aim of promoting the understanding and real-world implementation of One Health approach and contribution towards the Sustainable Development Goals for a healthy planet.Main text The proposed academic community would contribute to generating high-quality scientific evidence, distilling local experiences as well as fostering an interconnected One Health culture and mindset, among various stakeholders on different levels and in all sectors. The major scope of the community covers One Health governance, zoonotic diseases, food security, antimicrobial resistance, and climate change along with the research agenda to be developed. The academic community will be supported by two committees, including a strategic consultancy committee and a scientific steering committee, composed of influential scientists selected from the One Health information database. A workplan containing activities under six objectives is proposed to provide research support, strengthen local capacity, and enhance global participation.Conclusions The proposed academic community for One Health action is a crucial step towards enhancing communication, coordination, collaboration, and capacity building for the implementation of One Health. By bringing eminent global experts together, the academic community possesses the potential to generate scientific evidence and provide advice to local governments and international organizations, enabling the pursuit of common goals, collaborative policies, and solutions to misaligned interests. 展开更多
关键词 One Health Global health Academic community
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Optimal decision-making in relieving global high temperature-related disease burden by data-driven simulation
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作者 Xin-Chen Li Hao-Ran Qian +8 位作者 Yan-Yan zhang qi-yu zhang Jing-Shu Liu Hong-Yu Lai Wei-Guo Zheng Jian Sun Bo Fu Xiao-Nong Zhou Xiao-Xi zhang 《Infectious Disease Modelling》 2024年第2期618-633,共16页
The rapid acceleration of global warming has led to an increased burden of high temperature-related diseases(HTDs),highlighting the need for advanced evidence-based management strategies.We have developed a conceptual... The rapid acceleration of global warming has led to an increased burden of high temperature-related diseases(HTDs),highlighting the need for advanced evidence-based management strategies.We have developed a conceptual framework aimed at alleviating the global burden of HTDs,grounded in the One Health concept.This framework refines the impact pathway and establishes systematic data-driven models to inform the adoption of evidence-based decision-making,tailored to distinct contexts.We collected extensive national-level data from authoritative public databases for the years 2010–2019.The burdens of five categories of disease causes–cardiovascular diseases,infectious respiratory diseases,injuries,metabolic diseases,and non-infectious respiratory diseases–were designated as intermediate outcome variables.The cumulative burden of these five categories,referred to as the total HTD burden,was the final outcome variable.We evaluated the predictive performance of eight models and subsequently introduced twelve intervention measures,allowing us to explore optimal decision-making strategies and assess their corresponding contributions.Our model selection results demonstrated the superior performance of the Graph Neural Network(GNN)model across various metrics.Utilizing simulations driven by the GNN model,we identified a set of optimal intervention strategies for reducing disease burden,specifically tailored to the seven major regions:East Asia and Pacific,Europe and Central Asia,Latin America and the Caribbean,Middle East and North Africa,North America,South Asia,and Sub-Saharan Africa.Sectoral mitigation and adaptation measures,acting upon our categories of Infrastructure&Community,Ecosystem Resilience,and Health System Capacity,exhibited particularly strong performance for various regions and diseases.Seven out of twelve interventions were included in the optimal intervention package for each region,including raising low-carbon energy use,increasing energy intensity,improving livestock feed,expanding basic health care delivery coverage,enhancing health financing,addressing air pollution,and improving road infrastructure.The outcome of this study is a global decision-making tool,offering a systematic methodology for policymakers to develop targeted intervention strategies to address the increasingly severe challenge of HTDs in the context of global warming. 展开更多
关键词 High temperature-related diseases Data-driven simulation Optimal intervention Disease burden Graph neural network Global warming
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