Esophageal cancer is an upper gastrointestinal malignancy with a bleak prognosis.It is still being explored in depth due to its complex molecular mechanisms of occurrence and development.Lipids play a crucial role in ...Esophageal cancer is an upper gastrointestinal malignancy with a bleak prognosis.It is still being explored in depth due to its complex molecular mechanisms of occurrence and development.Lipids play a crucial role in cells by participating in energy supply,biofilm formation,and signal transduction processes,and lipid metabolic reprogramming also constitutes a significant characteristic of malignant tumors.More and more studies have found esophageal cancer has obvious lipid metabolism abnormalities throughout its beginning,progress,and treatment resistance.The inhibition of tumor growth and the enhancement of antitumor therapy efficacy can be achieved through the regulation of lipid metabolism.Therefore,we reviewed and analyzed the research results and latest findings for lipid metabolism and associated analysis techniques in esophageal cancer,and comprehensively proved the value of lipid metabolic reprogramming in the evolution and treatment resistance of esophageal cancer,as well as its significance in exploring potential therapeutic targets and biomarkers.展开更多
Support vehicles are part of the main body of airport ground operations,and their scheduling efficiency directly impacts flight delays.A mathematical model is constructed and the responsiveness of support vehicles for...Support vehicles are part of the main body of airport ground operations,and their scheduling efficiency directly impacts flight delays.A mathematical model is constructed and the responsiveness of support vehicles for current operational demands is proposed to study optimization algorithms for vehicle scheduling.The model is based on the constraint relationship of the initial operation time,time window,and gate position distribution,which gives an improvement to the ant colony algorithm(ACO).The impacts of the improved ACO as used for support vehicle optimization are compared and analyzed.The results show that the scheduling scheme of refueling trucks based on the improved ACO can reduce flight delays caused by refueling operations by 56.87%,indicating the improved ACO can improve support vehicle scheduling.Besides,the improved ACO can jump out of local optima,which can balance the working time of refueling trucks.This research optimizes the scheduling scheme of support vehicles under the existing conditions of airports,which has practical significance to fully utilize ground service resources,improve the efficiency of airport ground operations,and effectively reduce flight delays caused by ground service support.展开更多
BACKGROUND Slow transit constipation(STC)is a disorder with delayed colonic transit.Cinnamic acid(CA)is an organic acid in natural plants,such as Radix Scrophulariae(Xuan Shen),with low toxicity and biological activit...BACKGROUND Slow transit constipation(STC)is a disorder with delayed colonic transit.Cinnamic acid(CA)is an organic acid in natural plants,such as Radix Scrophulariae(Xuan Shen),with low toxicity and biological activities to modulate the intestinal microbiome.AIM To explore the potential effects of CA on the intestinal microbiome and the primary endogenous metabolites-short-chain fatty acids(SCFAs)and evaluate the therapeutic effects of CA in STC.METHODS Loperamide was applied to induce STC in mice.The treatment effects of CA on STC mice were assessed from the 24 h defecations,fecal moisture and intestinal transit rate.The enteric neurotransmitters:5-hydroxytryptamine(5-HT)and vasoactive intestinal peptide(VIP)were determined by the enzyme-linked immunosorbent assay.Hematoxylin-eosin and Alcian blue and Periodic acid Schiff staining were used to evaluate intestinal mucosa's histopathological performance and secretory function.16S rDNA was employed to analyze the composition and abundance of the intestinal microbiome.The SCFAs in stool samples were quantitatively detected by gas chromatography-mass spectrometry.RESULTS CA ameliorated the symptoms of STC and treated STC effectively.CA ameliorated the infiltration of neutrophils and lymphocytes,increased the number of goblet cells and acidic mucus secretion of the mucosa.In addition,CA significantly increased the concentration of 5-HT and reduced VIP.CA significantly improved the diversity and abundance of the beneficial microbiome.Furthermore,the production of SCFAs[including acetic acid(AA),butyric acid(BA),propionic acid(PA)and valeric acid(VA)]was significantly promoted by CA.The changed abundance of Firmicutes,Akkermansia,Lachnoclostridium,Monoglobus,UCG.005,Paenalcaligenes,Psychrobacter and Acinetobacter were involved in the production of AA,BA,PA and VA.CONCLUSION CA could treat STC effectively by ameliorating the composition and abundance of the intestinal microbiome to regulate the production of SCFAs.展开更多
Wavelength-tunable organic semiconductor lasers based on mechanically stretchable polydimethylsiloxane (PDMS) gratings were developed. The intrinsic stretchability of PDMS was explored to modulate the period of the di...Wavelength-tunable organic semiconductor lasers based on mechanically stretchable polydimethylsiloxane (PDMS) gratings were developed. The intrinsic stretchability of PDMS was explored to modulate the period of the distributed feedback gratings for fine tuning the lasing wavelength. Notably, elastic lasers based on three typical light-emitting molecules show com-parable lasing threshold values analogous to rigid devices and a continuous wavelength tunability of about 10 nm by mechanic-al stretching. In addition, the stretchability provides a simple solution for dynamically tuning the lasing wavelength in a spec-tral range that is challenging to achieve for inorganic counterparts. Our work has provided a simple and efficient method of fab-ricating tunable organic lasers that depend on stretchable distributed feedback gratings, demonstrating a significant step in the advancement of flexible organic optoelectronic devices.展开更多
The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect...The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect of low light on plant defense are still poorly understood.Here,using an RNA-seq approach,we found that the susceptibility of tomato to Pseudomonas syringae pv.tomato DC3000(Pst DC3000)under low light was associated with the oxidation-reduction process.Low light conditions exacerbated Pst DC3000-induced reactive oxygen species(ROS)accumulation and protein oxidation.Analysis of gene expression and enzyme activity of ascorbate peroxidase 2(APX2)and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light,whereas these genes and their associated enzyme activities were not responsive to pathogen inocula-tion under low light.Additionally,the reduced ascorbate to dehydroascorbate(AsA/DHA)ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation.Furthermore,the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions.Notably,this increased susceptibility could be significantly reduced by exogenous AsA treatment.Collectively,our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants.This study sheds light on the intricate relationship between light conditions,oxidative stress,and plant defense responses,and may pave the way for improved crop protection strategies in low light environments.展开更多
BACKGROUND This report delves into the diagnostic and therapeutic journey undertaken by a patient with Sneddon's syndrome(SS)and cerebral venous sinus thrombosis(CVST).Particular emphasis is placed on the comprehe...BACKGROUND This report delves into the diagnostic and therapeutic journey undertaken by a patient with Sneddon's syndrome(SS)and cerebral venous sinus thrombosis(CVST).Particular emphasis is placed on the comprehensive elucidation of SS's clinical manifestations,the intricate path to diagnosis,and the exploration of potential underlying mechanisms.CASE SUMMARY A 26-year-old woman presented with recurrent episodes of paroxysmal unilateral limb weakness accompanied by skin mottling,seizures,and cognitive impairment.Digital subtraction angiography revealed CVST.Despite negative antiphospholipid antibody results,skin biopsy indicated chronic inflammatory cell infiltration.The patient was treated using anticoagulation,antiepileptic therapy,and supportive care,which resulted in symptom improvement.The coexistence of SS and CVST is rare and the underlying pathophysiology remains uncertain.This case underscores the challenge in diagnosis and highlights the need for early clinical differentiation to facilitate accurate assessment and prompt intervention.CONCLUSION This article has reported and analyzed the clinical data,diagnosis,treatment,and prognosis of a case of SS with CVST and reviewed the relevant literature to improve the clinical understanding of this rare condition.展开更多
<strong>Objective: </strong>To understand the appetite and nutritional status of hospitalized patients in a tertiary A general hospital in Guangzhou, Guangdong Province. <strong>Methods:</strong&g...<strong>Objective: </strong>To understand the appetite and nutritional status of hospitalized patients in a tertiary A general hospital in Guangzhou, Guangdong Province. <strong>Methods:</strong> A cross-sectional survey of appetite and nutritional status assessment on inpatients in 44 wards of the hospital was conducted. Taking all “conscious patients hospitalized for more than 48 hours” in the hospital on November 25, 2020 as the survey subjects, the patients’ appetite, dietary intake, nutrition and nutritional support in the past week were investigated. <strong>Results: </strong>A total of 890 cases were investigated, among which 25 cases (2.81%) with missing data were excluded, and thus 865 investigated cases were considered effective. The incidence of nutritional risk was 28.67%, malnutrition 13.29%, external tube feeding nutrition 3.24%, parenteral nutrition 7.05%, and oral nutritional supplement 10.40%. The average score of appetite assessment was (6.99 ± 2.43) points. Among them, cases with appetite assessment scores < 5 points accounted for 15.84%, and 52 patient cases utilized appetite-improving drugs. Among the 137 patients with appetite scores < 5, only 7 patients utilized appetite-improving drugs. The patients’ dietary self-evaluation scores were averagely (4.08 ± 1.16) points, and the daily intake compliance rate of patients was 85.78%. Appetite assessment score was correlated with dietary intake score (<em>r</em> = 0.548) and daily intake compliance rate (<em>r</em> = 0.263) (<em>p</em> < 0.01). The differences in body weight, BMI, grip strength, albumin, and hemoglobin concentration of patients with different appetite states were statistically different (<em>p</em> < 0.01). Appetite was an influencing factor of weight change (<em>β</em> = <span style="white-space:nowrap;">−</span>0.079, <em>p</em> = 0.023). The difference between the appetite assessment scores and the daily intake compliance rates of patients with different nutritional support methods was statistically significant (<em>p</em> < 0.05);the individualized diet group had the highest appetite assessment score (8.57 ± 1.70), while the parenteral nutrition group had the lowest appetite assessment score (4.90 ± 2.99);the individualized diet group had the highest daily intake rate of 100%, followed by the parenteral nutrition group with 96.72%, and the regular diet group had the lowest rate of 84.02%. <strong>Conclusion: </strong>The appetite of hospitalized patients is closely related to nutritional status, and therefore, attention should be paid to the appetite status and nutritional status of hospitalized patients. Inpatients with different nutritional support methods should be given individualized appetite and nutritional interventions.展开更多
Soil enzyme activity plays an important role in the conversion of soil organic carbon into inorganic carbon, which is significant for the global carbon cycle. In this study, we investigated the soil enzyme activities ...Soil enzyme activity plays an important role in the conversion of soil organic carbon into inorganic carbon, which is significant for the global carbon cycle. In this study, we investigated the soil enzyme activities of two ligninolytic enzymes (peroxidase and polyphenol oxidase) and five non-ligninolytic enzymes (a-l,4-glucosidase (AG); 13-1,4-gluco- sidase (BG); N-acetyl-[3-glucosaminidase (NAG); ~3-D-cellobiosidase (CBH); and ^-xylosidase (BXYL)) in four plant communities of the Sangong River basin in Fukang, North Xinjiang, China. The four typical plant communities were dominated by Haloxylon ammodendron, Reaumuria soongonica, Salsola passerina, and Tamarix rarmosissima, respec- tively, with saline soils of varied alkalinity. The results showed that the soil peroxidase activity decreased seasonally. The activities of the five non-ligninolytic enzymes decreased with increasing soil depths, while those of the two ligninolytic enzymes did not show such a trend. In the four plant communities, BG had the highest activity among the five non-ligninolytic enzymes, and the activities of the two ligninolytic enzymes were higher than those of the four non-ligninolytic ones (AG, NAG, CBH, and BXYL). The community of H. ammodendron displayed the highest activity with respect to the two ligninolytic enzymes in most cases, but no significant differences were found among the four plant communities. The geometric mean of soil enzyme activities of the four plant communities was validated through an inde- pendently performed principal component analysis (PCA), which indicated that different plant communities had different soil enzyme activities. The correlation analysis showed that soil polyphenol oxidase activity was significantly positively correlated with the activities of the five non-ligninolytic enzymes. The soil pH value was positively correlated with the ac- tivities of all soil enzymes except peroxidase. Soil microbial carbon content also showed a significant positive correlation (P〈0.01) with the activities of all soil enzymes except polyphenol oxidase. The results suggested that the H. ammoden- dron community has the highest ability to utilize soil organic carbon, and glucoside could be the most extensively utilized non-ligninolytic carbon source in the saline soil of arid areas in Xinjiang.展开更多
Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by ...Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy.展开更多
[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the c...[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.展开更多
Si p^+n junction diodes operating in the mode of avalanche breakdown are capable of emitting light in the visible range of 400-900 nm. In this study, to realize the switching speed in the GHz range, we present a trans...Si p^+n junction diodes operating in the mode of avalanche breakdown are capable of emitting light in the visible range of 400-900 nm. In this study, to realize the switching speed in the GHz range, we present a transient model to shorten the carrier lifetime in the high electric field region by accumulating carriers in both p and n type regions. We also verify the optoelectronic characteristics by disclosing the related physical mechanisms behind the light emission phenomena. The emission of visible light by a monolithically integrated Si diode under the reverse bias is also discussed. The light is emitted as spatial sources by the defects located at the p-n junction of the reverse-biased diode. The influence of the defects on the electrical behavior is manifested as a current-dependent electroluminescence.展开更多
AIM:To investigate the effects of dexamethasone(DEX) and 1-(5-isoquinolinesulfonyl)-homopiperazine(HA1077) on actin cytoskeleton and β-catenin in cultured human trabecular meshwork(HTM) cells.METHODS: The H...AIM:To investigate the effects of dexamethasone(DEX) and 1-(5-isoquinolinesulfonyl)-homopiperazine(HA1077) on actin cytoskeleton and β-catenin in cultured human trabecular meshwork(HTM) cells.METHODS: The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX(1×10^-6mol/L) group,HA1077(3×10^-5mol/L)group,and DEX(1×10^-6mol/L) and HA1077(3×10^-5mol/L)group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS: In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks(CLANs),which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION: Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.展开更多
Lipid metabolism play an essential role in occurrence and development of asthma,and it can be disturbed by phthalate esters(PAEs)and organophosphate fame retardants(OPFRs).As a chronic infammatory respiratory disease,...Lipid metabolism play an essential role in occurrence and development of asthma,and it can be disturbed by phthalate esters(PAEs)and organophosphate fame retardants(OPFRs).As a chronic infammatory respiratory disease,the occurrence risk of childhood asthma is increased by PAEs and OPFRs exposure,but it remains not entirely clear how PAEs and OPFRs contribute the onset and progress of the disease.We have profiled the serum levels of PAEs and OPFRs congeners by liquid chromatography coupled with mass spectrometry,and its relationships with the dysregulation of lipid metabolism in asthmatic,bronchitic(acute infammation)and healthy(non-infammation)children.Eight PAEs and nine OPFRs congeners were found in the serum of children(1–5 years old)from Shenzhen,and their total median levels were 615.16 ng/m L and 17.06 ng/m L,respectively.Moreover,the serum levels of mono-methyl phthalate(MMP),tri-propyl phosphate(TPP)and tri-n-butyl phosphate(TNBP)were significant higher in asthmatic children than in healthy and bronchitic children as control.Thirty-one characteristic lipids and fatty acids of asthma were screened by machine-learning random forest model based on serum lipidome data,and the alterations of infammatory characteristic lipids and fatty acids including palmitic acids,12,13-Di HODE,14,21-Di HDHA,prostaglandin D2 and Lyso PA(18:2)showed significant correlated with high serum levels of MMP,TPP and TNBP.These results imply PAEs and OPFRs promote the occurrence of childhood asthma via disrupting infammatory lipid and fatty acid metabolism,and provide a novel sight for better understanding the effects of plastic additives on childhood asthma.展开更多
Cancer patients by immune checkpoint therapy have achieved long-term remission,with no recurrence of clinical symptoms of cancer for many years.Nevertheless,more than half of cancer patients are not responsive to this...Cancer patients by immune checkpoint therapy have achieved long-term remission,with no recurrence of clinical symptoms of cancer for many years.Nevertheless,more than half of cancer patients are not responsive to this therapy due to immune exhaustion.Here,we report a novel gene engineered exosome which is rationally designed by engineering PD1 gene and simultaneously enveloping an immune adjuvant imiquimod(PD1-Imi Exo)for boosting response of cancer immune checkpoint blockage therapy.The results showed that PD1-Imi Exo had a vesicular round shape(approximately 139 nm),revealed a significant targeting and a strong binding effect with both cancer cell and dendritic cell,and demonstrated a remarkable therapeutic efficacy in the melanoma-bearing mice and in the breast cancer-bearing mice.The mechanism was associated with two facts that PD1-Imi Exo blocked the binding of CD8^(+)T cell with cancer cell,displaying a PD1/PDL1 immune checkpoint blockage effect,and that imiquimod released from PD1-Imi Exo promoted the maturation of immature dendritic cell,exhibiting a reversing effect on the immune exhaustion through activating and restoring function of CD8^(+)T cell.In conclusion,the gene engineered exosome could be used for reversing T cell exhaustion in cancer immunotherapy.This study also offers a promising new strategy for enhancing PD1/PDL1 therapeutic efficacy,preventing tumor recurrence or metastasis after surgery by rebuilding the patients’immunity,thus consolidating the overall prognosis.展开更多
The urban power grid(UPG)combines transmission and distribution networks.Past studies on UPG congestion mitigation have primarily focused on relieving local congestion while ignoring large-scale energy transfer with s...The urban power grid(UPG)combines transmission and distribution networks.Past studies on UPG congestion mitigation have primarily focused on relieving local congestion while ignoring large-scale energy transfer with safety margins and load balancing.This situation is expected to worsen with the proliferation of renewable energy and electric vehicles.In this paper,a two-layer congestion mitigation framework is proposed,one which considers the congestion of the UPG with flexible topologies.In the upper-layer,the particle swarm optimization algorithm is employed to optimize the power supply distribution(PSD)of substation transformers.This is known as the upper-layer PSD.The lower-layer model recalculates the new PSD,known as the lower-layer PSD,based on the topology candidates.A candidate topology is at an optimum when the Euclidean distance mismatch between the upper-and lower-layer PSDs is the smallest.This optimum topology is tested by standard power flow to ascertain its feasibility.The optimum transitioning sequence between the initial and optimum topologies is also determined by the two-layer framework to minimize voltage deviation and line overloading of the UPG considering dynamic thermal rating.The proposed framework is tested on a 56-node test system.Results show that the proposed framework can significantly reduce congestion,maintain safety margins,and determine the optimum transitioning sequence.展开更多
Erratum to:SCIENCE CHINA Life Sciences,Volume 66,Issue 2:211-225(2022),https://doi.org/10.1007/s11427-021-2126-2 This paper contains an error in Figure 4C,where the representative images of filipin staining of retinal...Erratum to:SCIENCE CHINA Life Sciences,Volume 66,Issue 2:211-225(2022),https://doi.org/10.1007/s11427-021-2126-2 This paper contains an error in Figure 4C,where the representative images of filipin staining of retinal sections from 6-month-old mice were misused.We provide the correct picture for Figure 4C as follows.The statistical result in Figure 4D was corrected as well.This new Figure 4 does not affect the conclusion of this article.展开更多
Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast cancers.However,approaches to obtain cancer-reactive TCR-T cells have been unsucc...Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast cancers.However,approaches to obtain cancer-reactive TCR-T cells have been unsuccessful.Methods:Here,we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune fingerprints.Rare steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized mice.The human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and cytokines.To fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 days.Then,human T cells were processed for TCRβ-chain(TRB)sequencing analysis.After the repertoires had been constructed,features such as the fraction,diversity,and immune signature were investigated.Results:The results demonstrated an increase in diversity and clonality of T cells after treatment.The preferential usage and features of TRBV,TRBJ,and the V–J combination were also changed.The stress also induced highly clonal Science and Technology,Grant/Award Number:2021C03010;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004 expansion.Tumor burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized mice.Conclusions:We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T pools.Our platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells.It therefore has the potential to greatly benefit cancer treatment.展开更多
ObjectiveTo observe the clinical therapeutic effect on subthreshold depression of liver stagnation and spleen deficiency treated with acupuncture therapy for soothing the liver and regulating the mind combined with th...ObjectiveTo observe the clinical therapeutic effect on subthreshold depression of liver stagnation and spleen deficiency treated with acupuncture therapy for soothing the liver and regulating the mind combined with the well-timed auricular acupressure.MethodsA total of 34 patients were treated with acupuncture therapy for soothing the liver and regulating the mind combined with well-timed auricular acupressure.In acupuncture therapy for soothing the liver and regulating the mind,main acupoints included Baihui(BL20),Neiguan(PC6)and Taichong(LR3),and supplementary acupoints included Sishencong(EX-HN1),Shenting(GV24),Benshen(GB13),Zusanli(ST36),Ganshu(BL18)and Pishu(BL20).Acupuncture was given three times a week as one course and 4 courses of treatment were required.In well-timed auricular acupressure,auricular points included Xin(CO15),Pizhixia(AT4),Shenmen(TF4),Gan(CO12)and Pi(CO13).Acupressure with herbal seeds at those auricular points was applied at three time divisions,i.e.9:00 to 11:00,11:00 to 13:00 and 15:00 to 17:00 successively.The auricular acupressure with herbal seeds was given once every 3 days,the treatment for 1 week was 1 course and 4 courses were required.At the end of treatment and in 3 months of follow-up,the score of Hamilton depression scale-24(HAMD-24),syndrome score of traditional Chinese medicine(TCM),the score of Center for epidemiologic studies depression scale(CES-D)and the score of Short-form 36 item health survey(SF-36)were adopted to evaluate clinical therapeutic effect.ResultsAfter treatment and in three months of follow-up,HAMD-24 score,TCM syndrome score and CES-D score were all reduced as compared with the scores before treatment(all P<0.05)and SF-36 score was higher than the score before treatment,respectively(both P<0.05).In comparison of the scores after treatment with those in 3 months of follow-up,differences were not statistically significant(all P>0.05).Of 34 patients with subthreshold depression,8 cases were cured,15 cases effective remarkably,9 cases improved and 2 cases no effect.The total effective rate was 94.12%.ConclusionThe combined treatment of acupuncture therapy for soothing the liver and regulating the mind with the well-timed auricular acupressure can be benefit for relieving the symptoms and improves the quality of life in the patients with subthreshold depression of liver stagnation and spleen deficiency.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:22176195 and 82127801)National Key R&D Program of China(Grant No.:2022YFF0705003)+5 种基金the Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression(Grant No.:ZDSYS20220606100606014)the Guangdong Province Zhu Jiang Talents Plan,China(Grant No.:2021QN02Y028)the Natural Science Foundation of Guangdong Province,China(Grant No.:2021A1515010171)the Key Program of Fundamental Research in Shenzhen,China(Grant No.:JCYJ20210324115811031)the Sustainable Development Program of Shenzhen,China(Grant No.:KCXFZ202002011008124)the National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Shenzhen(Grant Nos.:SZ2020ZD002 and SZ2020QN005).
文摘Esophageal cancer is an upper gastrointestinal malignancy with a bleak prognosis.It is still being explored in depth due to its complex molecular mechanisms of occurrence and development.Lipids play a crucial role in cells by participating in energy supply,biofilm formation,and signal transduction processes,and lipid metabolic reprogramming also constitutes a significant characteristic of malignant tumors.More and more studies have found esophageal cancer has obvious lipid metabolism abnormalities throughout its beginning,progress,and treatment resistance.The inhibition of tumor growth and the enhancement of antitumor therapy efficacy can be achieved through the regulation of lipid metabolism.Therefore,we reviewed and analyzed the research results and latest findings for lipid metabolism and associated analysis techniques in esophageal cancer,and comprehensively proved the value of lipid metabolic reprogramming in the evolution and treatment resistance of esophageal cancer,as well as its significance in exploring potential therapeutic targets and biomarkers.
基金the Science and Technology Cooperation Research and Development Project of Sichuan Provincial Academy and University(Grant No.2019YFSY0024)the Key Research and Development Program in Sichuan Province of China(Grant No.2019YFG0050)the Natural Science Foundation of Guangxi Province of China(Grant No.AD19245021).
文摘Support vehicles are part of the main body of airport ground operations,and their scheduling efficiency directly impacts flight delays.A mathematical model is constructed and the responsiveness of support vehicles for current operational demands is proposed to study optimization algorithms for vehicle scheduling.The model is based on the constraint relationship of the initial operation time,time window,and gate position distribution,which gives an improvement to the ant colony algorithm(ACO).The impacts of the improved ACO as used for support vehicle optimization are compared and analyzed.The results show that the scheduling scheme of refueling trucks based on the improved ACO can reduce flight delays caused by refueling operations by 56.87%,indicating the improved ACO can improve support vehicle scheduling.Besides,the improved ACO can jump out of local optima,which can balance the working time of refueling trucks.This research optimizes the scheduling scheme of support vehicles under the existing conditions of airports,which has practical significance to fully utilize ground service resources,improve the efficiency of airport ground operations,and effectively reduce flight delays caused by ground service support.
基金Supported by the "333 Scientific Project" of Jiangsu Province in 2020, No. BRA2020237the Science and Technology Project of Suqian, Jiangsu Province in 2020, No. Z2020057
文摘BACKGROUND Slow transit constipation(STC)is a disorder with delayed colonic transit.Cinnamic acid(CA)is an organic acid in natural plants,such as Radix Scrophulariae(Xuan Shen),with low toxicity and biological activities to modulate the intestinal microbiome.AIM To explore the potential effects of CA on the intestinal microbiome and the primary endogenous metabolites-short-chain fatty acids(SCFAs)and evaluate the therapeutic effects of CA in STC.METHODS Loperamide was applied to induce STC in mice.The treatment effects of CA on STC mice were assessed from the 24 h defecations,fecal moisture and intestinal transit rate.The enteric neurotransmitters:5-hydroxytryptamine(5-HT)and vasoactive intestinal peptide(VIP)were determined by the enzyme-linked immunosorbent assay.Hematoxylin-eosin and Alcian blue and Periodic acid Schiff staining were used to evaluate intestinal mucosa's histopathological performance and secretory function.16S rDNA was employed to analyze the composition and abundance of the intestinal microbiome.The SCFAs in stool samples were quantitatively detected by gas chromatography-mass spectrometry.RESULTS CA ameliorated the symptoms of STC and treated STC effectively.CA ameliorated the infiltration of neutrophils and lymphocytes,increased the number of goblet cells and acidic mucus secretion of the mucosa.In addition,CA significantly increased the concentration of 5-HT and reduced VIP.CA significantly improved the diversity and abundance of the beneficial microbiome.Furthermore,the production of SCFAs[including acetic acid(AA),butyric acid(BA),propionic acid(PA)and valeric acid(VA)]was significantly promoted by CA.The changed abundance of Firmicutes,Akkermansia,Lachnoclostridium,Monoglobus,UCG.005,Paenalcaligenes,Psychrobacter and Acinetobacter were involved in the production of AA,BA,PA and VA.CONCLUSION CA could treat STC effectively by ameliorating the composition and abundance of the intestinal microbiome to regulate the production of SCFAs.
基金financial support from the National Natural Science Foundation of China (21835003, 91833304,21422402, 62274097, 21674050, 62004106)the National Key Basic Research Program of China (2014CB648300,2017YFB0404501)+11 种基金the Natural Science Foundation of Jiangsu Province (BE2019120, BK20160888)Program for Jiangsu Specially-Appointed Professor (RK030STP15001)the Six Talent Peaks Project of Jiangsu Province (TD-XCL-009)the333 Project of Jiangsu Province (BRA2017402)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (20KJB140005)China Postdoctoral Science Foundation (2020M671553)the NUPT"1311 Project"and Scientific Foundation (NY217169, NY215062, NY215107,NY217087)the Leading Talent of Technological Innovation of National Ten-Thousands Talents Program of Chinathe Excellent Scientific and Technological Innovative Teams of Jiangsu Higher Education Institutions (TJ217038)the Postgraduate Research&Practice Innovation Program of Jiangsu Province (SJCX21-0297)the Synergetic Innovation Center for Organic Electronics and Information Displaysthe Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘Wavelength-tunable organic semiconductor lasers based on mechanically stretchable polydimethylsiloxane (PDMS) gratings were developed. The intrinsic stretchability of PDMS was explored to modulate the period of the distributed feedback gratings for fine tuning the lasing wavelength. Notably, elastic lasers based on three typical light-emitting molecules show com-parable lasing threshold values analogous to rigid devices and a continuous wavelength tunability of about 10 nm by mechanic-al stretching. In addition, the stretchability provides a simple solution for dynamically tuning the lasing wavelength in a spec-tral range that is challenging to achieve for inorganic counterparts. Our work has provided a simple and efficient method of fab-ricating tunable organic lasers that depend on stretchable distributed feedback gratings, demonstrating a significant step in the advancement of flexible organic optoelectronic devices.
基金Thisworkwas supported by the National Natural Science Foundation of China(32172650)the Key Research and Development Program of Zhejiang Province(2021C02040).
文摘The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect of low light on plant defense are still poorly understood.Here,using an RNA-seq approach,we found that the susceptibility of tomato to Pseudomonas syringae pv.tomato DC3000(Pst DC3000)under low light was associated with the oxidation-reduction process.Low light conditions exacerbated Pst DC3000-induced reactive oxygen species(ROS)accumulation and protein oxidation.Analysis of gene expression and enzyme activity of ascorbate peroxidase 2(APX2)and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light,whereas these genes and their associated enzyme activities were not responsive to pathogen inocula-tion under low light.Additionally,the reduced ascorbate to dehydroascorbate(AsA/DHA)ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation.Furthermore,the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions.Notably,this increased susceptibility could be significantly reduced by exogenous AsA treatment.Collectively,our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants.This study sheds light on the intricate relationship between light conditions,oxidative stress,and plant defense responses,and may pave the way for improved crop protection strategies in low light environments.
文摘BACKGROUND This report delves into the diagnostic and therapeutic journey undertaken by a patient with Sneddon's syndrome(SS)and cerebral venous sinus thrombosis(CVST).Particular emphasis is placed on the comprehensive elucidation of SS's clinical manifestations,the intricate path to diagnosis,and the exploration of potential underlying mechanisms.CASE SUMMARY A 26-year-old woman presented with recurrent episodes of paroxysmal unilateral limb weakness accompanied by skin mottling,seizures,and cognitive impairment.Digital subtraction angiography revealed CVST.Despite negative antiphospholipid antibody results,skin biopsy indicated chronic inflammatory cell infiltration.The patient was treated using anticoagulation,antiepileptic therapy,and supportive care,which resulted in symptom improvement.The coexistence of SS and CVST is rare and the underlying pathophysiology remains uncertain.This case underscores the challenge in diagnosis and highlights the need for early clinical differentiation to facilitate accurate assessment and prompt intervention.CONCLUSION This article has reported and analyzed the clinical data,diagnosis,treatment,and prognosis of a case of SS with CVST and reviewed the relevant literature to improve the clinical understanding of this rare condition.
文摘<strong>Objective: </strong>To understand the appetite and nutritional status of hospitalized patients in a tertiary A general hospital in Guangzhou, Guangdong Province. <strong>Methods:</strong> A cross-sectional survey of appetite and nutritional status assessment on inpatients in 44 wards of the hospital was conducted. Taking all “conscious patients hospitalized for more than 48 hours” in the hospital on November 25, 2020 as the survey subjects, the patients’ appetite, dietary intake, nutrition and nutritional support in the past week were investigated. <strong>Results: </strong>A total of 890 cases were investigated, among which 25 cases (2.81%) with missing data were excluded, and thus 865 investigated cases were considered effective. The incidence of nutritional risk was 28.67%, malnutrition 13.29%, external tube feeding nutrition 3.24%, parenteral nutrition 7.05%, and oral nutritional supplement 10.40%. The average score of appetite assessment was (6.99 ± 2.43) points. Among them, cases with appetite assessment scores < 5 points accounted for 15.84%, and 52 patient cases utilized appetite-improving drugs. Among the 137 patients with appetite scores < 5, only 7 patients utilized appetite-improving drugs. The patients’ dietary self-evaluation scores were averagely (4.08 ± 1.16) points, and the daily intake compliance rate of patients was 85.78%. Appetite assessment score was correlated with dietary intake score (<em>r</em> = 0.548) and daily intake compliance rate (<em>r</em> = 0.263) (<em>p</em> < 0.01). The differences in body weight, BMI, grip strength, albumin, and hemoglobin concentration of patients with different appetite states were statistically different (<em>p</em> < 0.01). Appetite was an influencing factor of weight change (<em>β</em> = <span style="white-space:nowrap;">−</span>0.079, <em>p</em> = 0.023). The difference between the appetite assessment scores and the daily intake compliance rates of patients with different nutritional support methods was statistically significant (<em>p</em> < 0.05);the individualized diet group had the highest appetite assessment score (8.57 ± 1.70), while the parenteral nutrition group had the lowest appetite assessment score (4.90 ± 2.99);the individualized diet group had the highest daily intake rate of 100%, followed by the parenteral nutrition group with 96.72%, and the regular diet group had the lowest rate of 84.02%. <strong>Conclusion: </strong>The appetite of hospitalized patients is closely related to nutritional status, and therefore, attention should be paid to the appetite status and nutritional status of hospitalized patients. Inpatients with different nutritional support methods should be given individualized appetite and nutritional interventions.
基金National Basic Research Program of China (2009CB825103)National Natural Science Foundation of China (31200422)China’s Postdoctoral Science Foundation (2012M520455, 2013T60193)
文摘Soil enzyme activity plays an important role in the conversion of soil organic carbon into inorganic carbon, which is significant for the global carbon cycle. In this study, we investigated the soil enzyme activities of two ligninolytic enzymes (peroxidase and polyphenol oxidase) and five non-ligninolytic enzymes (a-l,4-glucosidase (AG); 13-1,4-gluco- sidase (BG); N-acetyl-[3-glucosaminidase (NAG); ~3-D-cellobiosidase (CBH); and ^-xylosidase (BXYL)) in four plant communities of the Sangong River basin in Fukang, North Xinjiang, China. The four typical plant communities were dominated by Haloxylon ammodendron, Reaumuria soongonica, Salsola passerina, and Tamarix rarmosissima, respec- tively, with saline soils of varied alkalinity. The results showed that the soil peroxidase activity decreased seasonally. The activities of the five non-ligninolytic enzymes decreased with increasing soil depths, while those of the two ligninolytic enzymes did not show such a trend. In the four plant communities, BG had the highest activity among the five non-ligninolytic enzymes, and the activities of the two ligninolytic enzymes were higher than those of the four non-ligninolytic ones (AG, NAG, CBH, and BXYL). The community of H. ammodendron displayed the highest activity with respect to the two ligninolytic enzymes in most cases, but no significant differences were found among the four plant communities. The geometric mean of soil enzyme activities of the four plant communities was validated through an inde- pendently performed principal component analysis (PCA), which indicated that different plant communities had different soil enzyme activities. The correlation analysis showed that soil polyphenol oxidase activity was significantly positively correlated with the activities of the five non-ligninolytic enzymes. The soil pH value was positively correlated with the ac- tivities of all soil enzymes except peroxidase. Soil microbial carbon content also showed a significant positive correlation (P〈0.01) with the activities of all soil enzymes except polyphenol oxidase. The results suggested that the H. ammoden- dron community has the highest ability to utilize soil organic carbon, and glucoside could be the most extensively utilized non-ligninolytic carbon source in the saline soil of arid areas in Xinjiang.
基金Funding from National Natural Science Foundation of China(Grant nos.81673367 and 81874303)Beijing Natural Science Foundation(Key Grant no.7181004).
文摘Natural exosomes can express specific proteins and carbohydratemolecules on the surface and hence have demonstrated the great potentials for gene therapy of cancer.However,the use of natural exosomes is restricted by their low transfection efficiency.Here,we report a novel targeting tLyp-1 exosome by gene recombinant engineering for delivery of siRNA to cancer and cancer stem cells.To reach such a purpose,the engineered tLyp-1-lamp2b plasmids were constructed and amplified in Escherichia coli.The tLyp-1-lamp2b plasmids were further used to transfect HEK293T tool cells and the targeting tLyp-1 exosomes were isolated from secretion of the transfected HEK293T cells.Afterwards,the artificially synthesized siRNA was encapsulated into targeting tLyp-1 exosomes by electroporation technology.Finally,the targeting siRNA tLyp-1 exosomes were used to transfect cancer or cancer stem cells.Results showed that the engineered targeting tLyp-1 exosomes had a nanosized structure(approximately 100 nm)and high transfection efficiency into lung cancer and cancer stem cells.The function verifications demonstrated that the targeting siRNA tLyp-1 exosomes were able to knock-down the target gene of cancer cells and to reduce the stemness of cancer stem cells.In conclusion,the targeting tLyp-1 exosomes are successfully engineered,and can be used for gene therapy with a high transfection efficiency.Therefore,the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy.
基金Supported by Guizhou Province Science and Technology Innovation Ability Promotion Special Project[2021]1Science and Technology Project of Guizhou Province([2021]YB201,[2019]3007,[2022]YB083)。
文摘[Objectives]Fifty two strains were identified and phylogenetically analyzed to solve the current phenomenon of"synonym and homonym"in morels.[Methods]Based on the ITS and LSU sequences of ribosomal DNA,the collected 52 morel samples were sequenced,and BLAST alignment was performed by NCBI to construct phylogenetic trees.[Results]The collected morel strains were mainly divided into two major groups:namely black morel and yellow morel groups,of which the black morel group was most,mainly including Morchella sextelata,Morchella importuna,Morchella crassipes,and Morchella eximia.There were very few yellow morel strains,including M.crassipes and M.eximia.[Conclusions]This study lays a foundation for the classification and research of morel germplasm resources,and provides a powerful reference value for the research and production of high-quality morel strains.
基金Project supported by the National Natural Science Foundation of China(Grant No.61704019)
文摘Si p^+n junction diodes operating in the mode of avalanche breakdown are capable of emitting light in the visible range of 400-900 nm. In this study, to realize the switching speed in the GHz range, we present a transient model to shorten the carrier lifetime in the high electric field region by accumulating carriers in both p and n type regions. We also verify the optoelectronic characteristics by disclosing the related physical mechanisms behind the light emission phenomena. The emission of visible light by a monolithically integrated Si diode under the reverse bias is also discussed. The light is emitted as spatial sources by the defects located at the p-n junction of the reverse-biased diode. The influence of the defects on the electrical behavior is manifested as a current-dependent electroluminescence.
基金Supported by the Natural Science Foundation of China (No.81300768 No.81371048+4 种基金 No.81670853)Science and Technology Department of Sichuan Province of China (No.2015HH0031)Health and Family Planning Commission of Sichuan Province of China (No.100539 No. 090505 No.090514)
文摘AIM:To investigate the effects of dexamethasone(DEX) and 1-(5-isoquinolinesulfonyl)-homopiperazine(HA1077) on actin cytoskeleton and β-catenin in cultured human trabecular meshwork(HTM) cells.METHODS: The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX(1×10^-6mol/L) group,HA1077(3×10^-5mol/L)group,and DEX(1×10^-6mol/L) and HA1077(3×10^-5mol/L)group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS: In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks(CLANs),which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION: Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.
基金supported by the National Natural Science Foundation of China (Nos.22076197,21707149 and 82127801)the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No.YJKYYQ20200034)+1 种基金Shenzhen Science and Technology Research Funding (Nos.JCYJ20210324115811031 and JCYJ20200109115405930)Guangdong Basic and Applied Basic Research Foundation (No.2020B1515120080)。
文摘Lipid metabolism play an essential role in occurrence and development of asthma,and it can be disturbed by phthalate esters(PAEs)and organophosphate fame retardants(OPFRs).As a chronic infammatory respiratory disease,the occurrence risk of childhood asthma is increased by PAEs and OPFRs exposure,but it remains not entirely clear how PAEs and OPFRs contribute the onset and progress of the disease.We have profiled the serum levels of PAEs and OPFRs congeners by liquid chromatography coupled with mass spectrometry,and its relationships with the dysregulation of lipid metabolism in asthmatic,bronchitic(acute infammation)and healthy(non-infammation)children.Eight PAEs and nine OPFRs congeners were found in the serum of children(1–5 years old)from Shenzhen,and their total median levels were 615.16 ng/m L and 17.06 ng/m L,respectively.Moreover,the serum levels of mono-methyl phthalate(MMP),tri-propyl phosphate(TPP)and tri-n-butyl phosphate(TNBP)were significant higher in asthmatic children than in healthy and bronchitic children as control.Thirty-one characteristic lipids and fatty acids of asthma were screened by machine-learning random forest model based on serum lipidome data,and the alterations of infammatory characteristic lipids and fatty acids including palmitic acids,12,13-Di HODE,14,21-Di HDHA,prostaglandin D2 and Lyso PA(18:2)showed significant correlated with high serum levels of MMP,TPP and TNBP.These results imply PAEs and OPFRs promote the occurrence of childhood asthma via disrupting infammatory lipid and fatty acid metabolism,and provide a novel sight for better understanding the effects of plastic additives on childhood asthma.
基金supported by the National Natural Science Foundation of China(No.82173752 and No.81874303).
文摘Cancer patients by immune checkpoint therapy have achieved long-term remission,with no recurrence of clinical symptoms of cancer for many years.Nevertheless,more than half of cancer patients are not responsive to this therapy due to immune exhaustion.Here,we report a novel gene engineered exosome which is rationally designed by engineering PD1 gene and simultaneously enveloping an immune adjuvant imiquimod(PD1-Imi Exo)for boosting response of cancer immune checkpoint blockage therapy.The results showed that PD1-Imi Exo had a vesicular round shape(approximately 139 nm),revealed a significant targeting and a strong binding effect with both cancer cell and dendritic cell,and demonstrated a remarkable therapeutic efficacy in the melanoma-bearing mice and in the breast cancer-bearing mice.The mechanism was associated with two facts that PD1-Imi Exo blocked the binding of CD8^(+)T cell with cancer cell,displaying a PD1/PDL1 immune checkpoint blockage effect,and that imiquimod released from PD1-Imi Exo promoted the maturation of immature dendritic cell,exhibiting a reversing effect on the immune exhaustion through activating and restoring function of CD8^(+)T cell.In conclusion,the gene engineered exosome could be used for reversing T cell exhaustion in cancer immunotherapy.This study also offers a promising new strategy for enhancing PD1/PDL1 therapeutic efficacy,preventing tumor recurrence or metastasis after surgery by rebuilding the patients’immunity,thus consolidating the overall prognosis.
基金supported by the Universiti Sains Malaysia,Research University Team(RUTeam)Grant Scheme(No.1001/PELECT/8580011).
文摘The urban power grid(UPG)combines transmission and distribution networks.Past studies on UPG congestion mitigation have primarily focused on relieving local congestion while ignoring large-scale energy transfer with safety margins and load balancing.This situation is expected to worsen with the proliferation of renewable energy and electric vehicles.In this paper,a two-layer congestion mitigation framework is proposed,one which considers the congestion of the UPG with flexible topologies.In the upper-layer,the particle swarm optimization algorithm is employed to optimize the power supply distribution(PSD)of substation transformers.This is known as the upper-layer PSD.The lower-layer model recalculates the new PSD,known as the lower-layer PSD,based on the topology candidates.A candidate topology is at an optimum when the Euclidean distance mismatch between the upper-and lower-layer PSDs is the smallest.This optimum topology is tested by standard power flow to ascertain its feasibility.The optimum transitioning sequence between the initial and optimum topologies is also determined by the two-layer framework to minimize voltage deviation and line overloading of the UPG considering dynamic thermal rating.The proposed framework is tested on a 56-node test system.Results show that the proposed framework can significantly reduce congestion,maintain safety margins,and determine the optimum transitioning sequence.
文摘Erratum to:SCIENCE CHINA Life Sciences,Volume 66,Issue 2:211-225(2022),https://doi.org/10.1007/s11427-021-2126-2 This paper contains an error in Figure 4C,where the representative images of filipin staining of retinal sections from 6-month-old mice were misused.We provide the correct picture for Figure 4C as follows.The statistical result in Figure 4D was corrected as well.This new Figure 4 does not affect the conclusion of this article.
基金National Natural Science Foundation of China,Grant/Award Numbers:82130003,81970158,82000180Zhejiang Provincial Key R&D Projects of Department of Science and Technology,Grant/Award Number:2021C03010Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004。
文摘Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast cancers.However,approaches to obtain cancer-reactive TCR-T cells have been unsuccessful.Methods:Here,we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune fingerprints.Rare steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized mice.The human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and cytokines.To fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 days.Then,human T cells were processed for TCRβ-chain(TRB)sequencing analysis.After the repertoires had been constructed,features such as the fraction,diversity,and immune signature were investigated.Results:The results demonstrated an increase in diversity and clonality of T cells after treatment.The preferential usage and features of TRBV,TRBJ,and the V–J combination were also changed.The stress also induced highly clonal Science and Technology,Grant/Award Number:2021C03010;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004 expansion.Tumor burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized mice.Conclusions:We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T pools.Our platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells.It therefore has the potential to greatly benefit cancer treatment.
基金Supported by 2020 Shandong Inheritance Project of Traditional Chinese Medicine Academic School:Shandong Health:2020-132。
文摘ObjectiveTo observe the clinical therapeutic effect on subthreshold depression of liver stagnation and spleen deficiency treated with acupuncture therapy for soothing the liver and regulating the mind combined with the well-timed auricular acupressure.MethodsA total of 34 patients were treated with acupuncture therapy for soothing the liver and regulating the mind combined with well-timed auricular acupressure.In acupuncture therapy for soothing the liver and regulating the mind,main acupoints included Baihui(BL20),Neiguan(PC6)and Taichong(LR3),and supplementary acupoints included Sishencong(EX-HN1),Shenting(GV24),Benshen(GB13),Zusanli(ST36),Ganshu(BL18)and Pishu(BL20).Acupuncture was given three times a week as one course and 4 courses of treatment were required.In well-timed auricular acupressure,auricular points included Xin(CO15),Pizhixia(AT4),Shenmen(TF4),Gan(CO12)and Pi(CO13).Acupressure with herbal seeds at those auricular points was applied at three time divisions,i.e.9:00 to 11:00,11:00 to 13:00 and 15:00 to 17:00 successively.The auricular acupressure with herbal seeds was given once every 3 days,the treatment for 1 week was 1 course and 4 courses were required.At the end of treatment and in 3 months of follow-up,the score of Hamilton depression scale-24(HAMD-24),syndrome score of traditional Chinese medicine(TCM),the score of Center for epidemiologic studies depression scale(CES-D)and the score of Short-form 36 item health survey(SF-36)were adopted to evaluate clinical therapeutic effect.ResultsAfter treatment and in three months of follow-up,HAMD-24 score,TCM syndrome score and CES-D score were all reduced as compared with the scores before treatment(all P<0.05)and SF-36 score was higher than the score before treatment,respectively(both P<0.05).In comparison of the scores after treatment with those in 3 months of follow-up,differences were not statistically significant(all P>0.05).Of 34 patients with subthreshold depression,8 cases were cured,15 cases effective remarkably,9 cases improved and 2 cases no effect.The total effective rate was 94.12%.ConclusionThe combined treatment of acupuncture therapy for soothing the liver and regulating the mind with the well-timed auricular acupressure can be benefit for relieving the symptoms and improves the quality of life in the patients with subthreshold depression of liver stagnation and spleen deficiency.