We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(...We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(DL-VHQPI).The method,incorporating a conventional deep neural network into a complete physical model utilizing the idea of residual compensation,reliably and robustly recovers the quantitative phase information of the test objects.It can significantly alleviate spectrum-overlapping-caused phase artifacts under the slightly off-axis digital holographic system.Compared to the conventional end-to-end networks(without a physical model),the proposed method can reduce the dataset size dramatically while maintaining the imaging quality and model generalization.The DL-VHQPI is quantitatively studied by numerical simulation.The live-cell experiment is designed to demonstrate the method's practicality in biological research.The proposed idea of the deep learning-assisted physical model might be extended to diverse computational imaging techniques.展开更多
BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evalua...BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.展开更多
Metabolic syndrome(MetS)is an individual accumulation of multiple abnormal metabolic indicators,with insulin resistance and central obesity at its core.Adipose tissue is the core component of MetS,and adipokines secre...Metabolic syndrome(MetS)is an individual accumulation of multiple abnormal metabolic indicators,with insulin resistance and central obesity at its core.Adipose tissue is the core component of MetS,and adipokines secreted by various adipocytes play a crucial role in its regulatory mechanism.In this paper,we searched the literature from 2020 to 2022 on Pubmed and Knowledgeweb using the keywords of“metabolic syndrome,”“adipose tissue”and“adipokines”.We mainly explored the mechanisms of leptin,lipocalin,resistin,brown adipose tissue and beige adipose tissue secreted by white adipose tissue in regulating insulin resistance,inhibiting inflammatory response and neurohormonal regulation of lipid and glucose metabolism.In this paper,under various studies on lipids for MetS,the metabolic mechanisms of various adipose tissues,adipocytes,and adipokines were investigated systematically and retrospectively.It was found that the regulation of adipokines and,thus,treatment of MetS by drugs is less and needs further exploration.展开更多
Two new terpenes,triptobenzene P（1）and wilforone（2）were isolated from Tripterygium wilfordii,as well as 10 known terpenes.Their structures were elucidated by spectroscopic methods.Compounds 2-4,8,10,and 11 showed ...Two new terpenes,triptobenzene P（1）and wilforone（2）were isolated from Tripterygium wilfordii,as well as 10 known terpenes.Their structures were elucidated by spectroscopic methods.Compounds 2-4,8,10,and 11 showed significant immunosuppressive activities.展开更多
AIM： To generate a SV40Tag transgenic tumor animal model and to study the mechanism underlying tumorigenesis. METHODS： A mammary gland expression vector containing SV40Tag DNA was generated. Transgene fragments were...AIM： To generate a SV40Tag transgenic tumor animal model and to study the mechanism underlying tumorigenesis. METHODS： A mammary gland expression vector containing SV40Tag DNA was generated. Transgene fragments were microinjeted into fertilized eggs of FVB mice. The genetically manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. PCR and Northern blot analysis were used for genotype analysis of F1 and F2 mice. Transgene expression was detected by RT-PCR and immunohistochemistry. RESULTS： SV40Tag gene was detected in two lines of transgenic mice. One of them delivered the transgene to F1 and a tumor was found in the pancreas of these mice. RT-PCR and immunohistochemistry showed that SV40Tag gene was expressed in the tumor. Pathological characterization of the transgenic mice demonstrated that the tumor belonged to pancreatic cystic neoplasm. CONCLUSION： SV40Tag transgenic mouse model can be successfully established. The transgenic mice develop a pancreatic tumor, which can be used for investigation of the molecular mechanism of tumorigenesis in vivo.展开更多
Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant,anticancer,and antimalarial activities.The transcriptional regulation of flavonoid biosynthesi...Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant,anticancer,and antimalarial activities.The transcriptional regulation of flavonoid biosynthesis in A.annua has not been well-studied.In this study,we identified a YABBY family transcription factor,AaYABBY5,as a positive regulator of anthocyanin and total flavonoid contents in A.annua.AaYABBY5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),chalcone isomerase(CHI),and flavonol synthase(FLS)genes.A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene,with reporters AaPAL,AaCHS,AaCHI,and AaUFGT.The yeast one-hybrid system further confirmed the direct activation of these promoters by AaYABBY5.Gene expression analysis of stably transformed AaYABBY5 overexpression,AaYABBY5 antisense,and control plants revealed a significant increase in the expression of AaPAL,AaCHS,AaCHI,AaFLS,AaFSII,AaLDOX,and AaUFGT in AaYABBY5 overexpression plants.Moreover,their total flavonoid content and anthocyanin content were also found to increase.AaYABBY5 antisense plants showed a significant decrease in the expression of flavonoid biosynthetic genes,as well as a decrease in anthocyanin and total flavonoid contents.In addition,phenotypic analysis revealed deep purple-pigmented stems,an increase in the leaf lamina size,and higher trichome densities in AaYABBY5 overexpression plants.Together,these data proved that AaYABBY5 is a positive regulator of flavonoid biosynthesis in A.annua.Our study provides candidate transcription factors for the improvement of flavonoid concentrations in A.annua and can be further extended to elucidate its mechanism of regulating trichome development.展开更多
Two new terpenes,wilfornine H(1) and triptobenzene Q(2) were isolated from Tripterygium wilfordii,as well as 11 terpenes. Their structures were elucidated by spectroscopic methods.Compounds 5,9-13 showed significant i...Two new terpenes,wilfornine H(1) and triptobenzene Q(2) were isolated from Tripterygium wilfordii,as well as 11 terpenes. Their structures were elucidated by spectroscopic methods.Compounds 5,9-13 showed significant immunosuppressive activities.展开更多
BACKGROUND Primary pulmonary enteric adenocarcinoma(PEAC)is a very rare subtype of invasive adenocarcinoma,and there have been no large studies on PEAC to date.Therefore,it is necessary to obtain much more information...BACKGROUND Primary pulmonary enteric adenocarcinoma(PEAC)is a very rare subtype of invasive adenocarcinoma,and there have been no large studies on PEAC to date.Therefore,it is necessary to obtain much more information about the clinical and pathological features,diagnosis,differential diagnosis,and treatment of PEAC.CASE SUMMARY All clinical data of six patients with confirmed PEAC from 2013 to 2018 were collected,and data on diagnosis,differential diagnosis,and treatment of PEAC are discussed combined with all the associated literature.The mean age of six patients was 64.0±5.6(59-73)years old.Their clinical manifestations were heterogeneous,and during their disease course,there were no gastrointestinal symptoms.There was no evidence from colonoscopy or imaging studies to suggest digestive tract tumors or new metastases.The most commonly mutated gene was KRAS(50.0%),and the pathological features of the six cases were similar to those of colorectal cancer.CDX2(83.3%)and CK7(66.7%)had the highest positive rates upon immunohistochemical examination.In the associated literature,252 cases were identified,and the most commonly mutated gene was KRAS(42.9%).Additionally,CDX2(68.3%)and CK7(85.8%)had the highest positive rates.Patients mainly received surgery,chemotherapy,and radiotherapy,immunotherapy was not included.CONCLUSION Positive results for CDX2 and CK7 play an important role in the diagnosis and differential diagnosis of PEAC,and immunotherapy or targeted therapy focused on KRAS needs to be further studied for the treatment of PEAC.展开更多
To summarize the application and modern studies on health Qigong Baduanjin by domestic and foreign scholars,combined them with clinical practice of mobile hospital in Wuhan,we explore the prevention,treatment,and reco...To summarize the application and modern studies on health Qigong Baduanjin by domestic and foreign scholars,combined them with clinical practice of mobile hospital in Wuhan,we explore the prevention,treatment,and recovery of Baduanjin in the war against COVID‑19.On the basis of studying ancient classics,such as Huangdi’s Internal Classic,and the concrete exercise methods of Baduanjin,we propose three ideas:prevention before disease onset,promoting healing during illness,and strengthening the body after recovery.We aimed to give full play to Baduanjin in the battle against COVID‑19 to popularize correct prevention and health knowledge for the patients and the healthy as well as to provide a reference for medical workers to use Traditional Chinese Medicine to prevention and treatment.展开更多
Radiation cystitis is one of the major complications following radiotherapy for cervical cancer. However, spontaneous intraperitoneal bladder rupture as a result of radiation cystitis following radiotherapy for cervic...Radiation cystitis is one of the major complications following radiotherapy for cervical cancer. However, spontaneous intraperitoneal bladder rupture as a result of radiation cystitis following radiotherapy for cervical cancer is extremely rare. Case presentation: We report a 52-year-old patient who received radiation therapy for cervical cancer 15 years prior to presentation. Eight years prior to presentation, she developed recurrent abdominal distension, oliguria, and ascites. Following ascites drainage and supportive treatment, all symptoms were relieved. However, all symptoms subsequently recurred every few months. The patient underwent exploratory laparotomy twice. The first exploratory laparotomy in July 2015 found no specific abnormalities. The second exploratory laparotomy in November 2016 found an intraperitoneal bladder rupture, and the patient underwent surgical repair. The ascites subsequently resolved. Conclusion: The occurrence of spontaneous intraperitoneal bladder rupture after radiation therapy for cervical cancer is rare. The prognosis is good when diagnosis and treatment are prompt.展开更多
Objective: To observe clinical application of remifentanil combined with propofol anesthesia and fentanyl combined with propofol anesthesia in patients with brain surgery, and analyze the change of hemodynamics and se...Objective: To observe clinical application of remifentanil combined with propofol anesthesia and fentanyl combined with propofol anesthesia in patients with brain surgery, and analyze the change of hemodynamics and serum IL-1β, IL-6 and TNF-α before and after operation. Methods: A total of 90 cases of patients with brain surgery were randomly divided into control group and observation group according to lottery. The control group was given fentanyl combined with propofol anesthesia and the observation group was given remifentanil combined with propofol anesthesia, other combined anesthetic drug was same in both groups. Hemodynamics of all subjects before tracheal intubation (T1), after tracheal intubation (T2), when shin incision (T3) and when extubation (T4) and serum IL-1β, IL-6 and TNF-α before and after surgery 24 hours were measured. Results: There was no significant difference in hemodynamic indexes of all patients in both groups at T1. At T2, T3 and T4, heart rate (HR) and mean arterial pressure (MAP) of the observation group were significantly lower than those in the control group at same period, however blood oxygen saturation (SpO2) was higher than that in the control group at corresponding period, it was significant difference. At T2, T3 and T4, observation indexes HR and MAP of the control group were significantly higher than those at T1, while SpO2 was significantly lower than T1, there was statistical significant difference. While there was no significant difference in HR, MAP and SpO2 in observation group at T1, T2, T3 and T4. There was no significant differences in serum IL-1β, IL-6 and TNF-α levels between the control group and the observation group before surgery. After 24 h of operation, the levels of IL-1β, IL-6 and TNF-α in both groups were higher than those before operation;Moreover levels of IL-1β, IL-6 and TNF-α in observation group were significantly lower than those in the control group at same period after 24 h of operation it was statistical significant difference. Conclusion: Remifentanil combined with propofol anesthesia was able to maintain hemodynamic stability and reduce concentration of serum inflammatory factors, which was one of the potential effective anesthetic compounds.展开更多
Background: Discrete clinical and pathological subtypes of Alzheimer’s disease (AD) with variable presentations and rates of progression are well known. These subtypes may have specific patterns of regional brain atr...Background: Discrete clinical and pathological subtypes of Alzheimer’s disease (AD) with variable presentations and rates of progression are well known. These subtypes may have specific patterns of regional brain atrophy, which are identifiable on MRI scans. Methods: To examine distinct regions which had distinct underlying patterns of cortical atrophy, factor analytic techniques applied to structural MRI volumetric data from cognitively normal (CN) (n = 202), amnestic mild cognitive impairment (aMCI) (n = 333) or mild AD (n = 146) subjects, in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database was applied. This revealed the existence of two neocortical (NeoC-1 and NeoC-2), and a limbic cluster of atrophic brain regions. The frequency and clinical correlates of these regional patterns of atrophy were evaluated among the three diagnostic groups, and the rates of progression from aMCI to AD, over 24 months were evaluated. Results: Discernable patterns of regional atrophy were observed in about 29% of CN, 55% of aMCI and 83% of AD subjects. Heterogeneity in clinical presentation and APOE ε4 frequency were associated with regional patterns of atrophy on MRI scans. The most rapid progression rates to dementia among aMCI subjects (n = 224), over a 24-month period, were in those with NeoC-1 regional impairment (68.2%), followed by the Limbic regional impairment (48.8%). The same pattern of results was observed when only aMCI amyloid positive subjects were examined. Conclusions: The neuroimaging results closely parallel findings described recently among AD patients with the hippocampal sparing and limbic subtypes of AD neuropathology at autopsy. We conclude that NeoC-1, Limbic and other patterns of MRI atrophy may be useful markers for predicting the rate of progression of aMCI to AD and could have utility selecting individuals at higher risk for progression in clinical trials.展开更多
基金We are grateful for financial supports from the National Natural Science Foundation of China(61905115,62105151,62175109,U21B2033,62227818)Leading Technology of Jiangsu Basic Research Plan(BK20192003)+5 种基金Youth Foundation of Jiangsu Province(BK20190445,BK20210338)Biomedical Competition Foundation of Jiangsu Province(BE2022847)Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039)Fundamental Research Funds for the Central Universities(30920032101)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201)National Science Center,Poland(2020/37/B/ST7/03629).The authors thank F.Sun for her contribution to this paper in terms of language expression and grammatical correction.
文摘We propose a high-accuracy artifacts-free single-frame digital holographic phase demodulation scheme for relatively lowcarrier frequency holograms-deep learning assisted variational Hilbert quantitative phase imaging(DL-VHQPI).The method,incorporating a conventional deep neural network into a complete physical model utilizing the idea of residual compensation,reliably and robustly recovers the quantitative phase information of the test objects.It can significantly alleviate spectrum-overlapping-caused phase artifacts under the slightly off-axis digital holographic system.Compared to the conventional end-to-end networks(without a physical model),the proposed method can reduce the dataset size dramatically while maintaining the imaging quality and model generalization.The DL-VHQPI is quantitatively studied by numerical simulation.The live-cell experiment is designed to demonstrate the method's practicality in biological research.The proposed idea of the deep learning-assisted physical model might be extended to diverse computational imaging techniques.
基金This study is registered at https://www.clinicaltrials.gov/.The registration identification number is NCT04639323.
文摘BACKGROUND We invented Endoscopic Ruler,a new endoscopic device to measure the size of varices in patients with cirrhosis and portal hypertension.AIM To assess the feasibility and safety of Endoscopic Ruler,and evaluate the agreement on identifying large oesophageal varices(OV)between Endoscopic Ruler and the endoscopists,as well as the interobserver agreement on diagnosing large OV using Endoscopic Ruler.METHODS We prospectively and consecutively enrolled patients with cirrhosis from 11 hospitals,all of whom got esophagogastroduodenoscopy(EGD)with Endoscopic Ruler.The primary study outcome was a successful measurement of the size of varices using Endoscopic Ruler.The secondary outcomes included adverse events,operation time,the agreement of identifying large OV between the objective measurement of Endoscopic Ruler and the empirical reading of endoscopists,together with the interobserver agreement on diagnosing large OV by Endoscopic Ruler.RESULTS From November 2020 to April 2022,a total of 120 eligible patients with cirrhosis were recruited and all of them underwent EGD examinations with Endoscopic Ruler successfully without any adverse event.The median operation time of Endoscopic Ruler was 3.00 min[interquartile range(IQR):3.00 min].The kappa value between Endoscopic Ruler and the endoscopists while detecting large OV was 0.52,demonstrating a moderate agreement.The kappa value for diagnosing large OV using Endoscopic Ruler among the six independent observers was 0.77,demonstrating a substantial agreement.CONCLUSION The data demonstrates that Endoscopic Ruler is feasible and safe for measuring the size of varices in patients with cirrhosis and portal hypertension.Endoscopic Ruler is potential to promote the clinical practice of the two-grade classification system of OV.
文摘Metabolic syndrome(MetS)is an individual accumulation of multiple abnormal metabolic indicators,with insulin resistance and central obesity at its core.Adipose tissue is the core component of MetS,and adipokines secreted by various adipocytes play a crucial role in its regulatory mechanism.In this paper,we searched the literature from 2020 to 2022 on Pubmed and Knowledgeweb using the keywords of“metabolic syndrome,”“adipose tissue”and“adipokines”.We mainly explored the mechanisms of leptin,lipocalin,resistin,brown adipose tissue and beige adipose tissue secreted by white adipose tissue in regulating insulin resistance,inhibiting inflammatory response and neurohormonal regulation of lipid and glucose metabolism.In this paper,under various studies on lipids for MetS,the metabolic mechanisms of various adipose tissues,adipocytes,and adipokines were investigated systematically and retrospectively.It was found that the regulation of adipokines and,thus,treatment of MetS by drugs is less and needs further exploration.
文摘Two new terpenes,triptobenzene P（1）and wilforone（2）were isolated from Tripterygium wilfordii,as well as 10 known terpenes.Their structures were elucidated by spectroscopic methods.Compounds 2-4,8,10,and 11 showed significant immunosuppressive activities.
基金Supported by the National Key Technologies Research and Development Program of China during The 10th Five-Year Plan Period, No2001BA70113.
文摘AIM： To generate a SV40Tag transgenic tumor animal model and to study the mechanism underlying tumorigenesis. METHODS： A mammary gland expression vector containing SV40Tag DNA was generated. Transgene fragments were microinjeted into fertilized eggs of FVB mice. The genetically manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. PCR and Northern blot analysis were used for genotype analysis of F1 and F2 mice. Transgene expression was detected by RT-PCR and immunohistochemistry. RESULTS： SV40Tag gene was detected in two lines of transgenic mice. One of them delivered the transgene to F1 and a tumor was found in the pancreas of these mice. RT-PCR and immunohistochemistry showed that SV40Tag gene was expressed in the tumor. Pathological characterization of the transgenic mice demonstrated that the tumor belonged to pancreatic cystic neoplasm. CONCLUSION： SV40Tag transgenic mouse model can be successfully established. The transgenic mice develop a pancreatic tumor, which can be used for investigation of the molecular mechanism of tumorigenesis in vivo.
基金This work was supported by the National Key R&D Program of China(2018YFA0900600)the Bill&Melinda Gates Foundation(OPP1199872)+1 种基金the SJTU Global Strategic Partnership Fund(2020SJTU-CORNELL)the SJTU Trans-med Awards Research Program(20190104).
文摘Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant,anticancer,and antimalarial activities.The transcriptional regulation of flavonoid biosynthesis in A.annua has not been well-studied.In this study,we identified a YABBY family transcription factor,AaYABBY5,as a positive regulator of anthocyanin and total flavonoid contents in A.annua.AaYABBY5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),chalcone isomerase(CHI),and flavonol synthase(FLS)genes.A transient dual-luciferase assay in Nicotiana bethamiana with the AaYABBY5 effector showed a significant increase in the activity of the downstream LUC gene,with reporters AaPAL,AaCHS,AaCHI,and AaUFGT.The yeast one-hybrid system further confirmed the direct activation of these promoters by AaYABBY5.Gene expression analysis of stably transformed AaYABBY5 overexpression,AaYABBY5 antisense,and control plants revealed a significant increase in the expression of AaPAL,AaCHS,AaCHI,AaFLS,AaFSII,AaLDOX,and AaUFGT in AaYABBY5 overexpression plants.Moreover,their total flavonoid content and anthocyanin content were also found to increase.AaYABBY5 antisense plants showed a significant decrease in the expression of flavonoid biosynthetic genes,as well as a decrease in anthocyanin and total flavonoid contents.In addition,phenotypic analysis revealed deep purple-pigmented stems,an increase in the leaf lamina size,and higher trichome densities in AaYABBY5 overexpression plants.Together,these data proved that AaYABBY5 is a positive regulator of flavonoid biosynthesis in A.annua.Our study provides candidate transcription factors for the improvement of flavonoid concentrations in A.annua and can be further extended to elucidate its mechanism of regulating trichome development.
文摘Two new terpenes,wilfornine H(1) and triptobenzene Q(2) were isolated from Tripterygium wilfordii,as well as 11 terpenes. Their structures were elucidated by spectroscopic methods.Compounds 5,9-13 showed significant immunosuppressive activities.
基金Supported by Medicine and Health Project of Zhejiang Province,China,No.2018KY049。
文摘BACKGROUND Primary pulmonary enteric adenocarcinoma(PEAC)is a very rare subtype of invasive adenocarcinoma,and there have been no large studies on PEAC to date.Therefore,it is necessary to obtain much more information about the clinical and pathological features,diagnosis,differential diagnosis,and treatment of PEAC.CASE SUMMARY All clinical data of six patients with confirmed PEAC from 2013 to 2018 were collected,and data on diagnosis,differential diagnosis,and treatment of PEAC are discussed combined with all the associated literature.The mean age of six patients was 64.0±5.6(59-73)years old.Their clinical manifestations were heterogeneous,and during their disease course,there were no gastrointestinal symptoms.There was no evidence from colonoscopy or imaging studies to suggest digestive tract tumors or new metastases.The most commonly mutated gene was KRAS(50.0%),and the pathological features of the six cases were similar to those of colorectal cancer.CDX2(83.3%)and CK7(66.7%)had the highest positive rates upon immunohistochemical examination.In the associated literature,252 cases were identified,and the most commonly mutated gene was KRAS(42.9%).Additionally,CDX2(68.3%)and CK7(85.8%)had the highest positive rates.Patients mainly received surgery,chemotherapy,and radiotherapy,immunotherapy was not included.CONCLUSION Positive results for CDX2 and CK7 play an important role in the diagnosis and differential diagnosis of PEAC,and immunotherapy or targeted therapy focused on KRAS needs to be further studied for the treatment of PEAC.
文摘To summarize the application and modern studies on health Qigong Baduanjin by domestic and foreign scholars,combined them with clinical practice of mobile hospital in Wuhan,we explore the prevention,treatment,and recovery of Baduanjin in the war against COVID‑19.On the basis of studying ancient classics,such as Huangdi’s Internal Classic,and the concrete exercise methods of Baduanjin,we propose three ideas:prevention before disease onset,promoting healing during illness,and strengthening the body after recovery.We aimed to give full play to Baduanjin in the battle against COVID‑19 to popularize correct prevention and health knowledge for the patients and the healthy as well as to provide a reference for medical workers to use Traditional Chinese Medicine to prevention and treatment.
文摘Radiation cystitis is one of the major complications following radiotherapy for cervical cancer. However, spontaneous intraperitoneal bladder rupture as a result of radiation cystitis following radiotherapy for cervical cancer is extremely rare. Case presentation: We report a 52-year-old patient who received radiation therapy for cervical cancer 15 years prior to presentation. Eight years prior to presentation, she developed recurrent abdominal distension, oliguria, and ascites. Following ascites drainage and supportive treatment, all symptoms were relieved. However, all symptoms subsequently recurred every few months. The patient underwent exploratory laparotomy twice. The first exploratory laparotomy in July 2015 found no specific abnormalities. The second exploratory laparotomy in November 2016 found an intraperitoneal bladder rupture, and the patient underwent surgical repair. The ascites subsequently resolved. Conclusion: The occurrence of spontaneous intraperitoneal bladder rupture after radiation therapy for cervical cancer is rare. The prognosis is good when diagnosis and treatment are prompt.
文摘Objective: To observe clinical application of remifentanil combined with propofol anesthesia and fentanyl combined with propofol anesthesia in patients with brain surgery, and analyze the change of hemodynamics and serum IL-1β, IL-6 and TNF-α before and after operation. Methods: A total of 90 cases of patients with brain surgery were randomly divided into control group and observation group according to lottery. The control group was given fentanyl combined with propofol anesthesia and the observation group was given remifentanil combined with propofol anesthesia, other combined anesthetic drug was same in both groups. Hemodynamics of all subjects before tracheal intubation (T1), after tracheal intubation (T2), when shin incision (T3) and when extubation (T4) and serum IL-1β, IL-6 and TNF-α before and after surgery 24 hours were measured. Results: There was no significant difference in hemodynamic indexes of all patients in both groups at T1. At T2, T3 and T4, heart rate (HR) and mean arterial pressure (MAP) of the observation group were significantly lower than those in the control group at same period, however blood oxygen saturation (SpO2) was higher than that in the control group at corresponding period, it was significant difference. At T2, T3 and T4, observation indexes HR and MAP of the control group were significantly higher than those at T1, while SpO2 was significantly lower than T1, there was statistical significant difference. While there was no significant difference in HR, MAP and SpO2 in observation group at T1, T2, T3 and T4. There was no significant differences in serum IL-1β, IL-6 and TNF-α levels between the control group and the observation group before surgery. After 24 h of operation, the levels of IL-1β, IL-6 and TNF-α in both groups were higher than those before operation;Moreover levels of IL-1β, IL-6 and TNF-α in observation group were significantly lower than those in the control group at same period after 24 h of operation it was statistical significant difference. Conclusion: Remifentanil combined with propofol anesthesia was able to maintain hemodynamic stability and reduce concentration of serum inflammatory factors, which was one of the potential effective anesthetic compounds.
文摘Background: Discrete clinical and pathological subtypes of Alzheimer’s disease (AD) with variable presentations and rates of progression are well known. These subtypes may have specific patterns of regional brain atrophy, which are identifiable on MRI scans. Methods: To examine distinct regions which had distinct underlying patterns of cortical atrophy, factor analytic techniques applied to structural MRI volumetric data from cognitively normal (CN) (n = 202), amnestic mild cognitive impairment (aMCI) (n = 333) or mild AD (n = 146) subjects, in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database was applied. This revealed the existence of two neocortical (NeoC-1 and NeoC-2), and a limbic cluster of atrophic brain regions. The frequency and clinical correlates of these regional patterns of atrophy were evaluated among the three diagnostic groups, and the rates of progression from aMCI to AD, over 24 months were evaluated. Results: Discernable patterns of regional atrophy were observed in about 29% of CN, 55% of aMCI and 83% of AD subjects. Heterogeneity in clinical presentation and APOE ε4 frequency were associated with regional patterns of atrophy on MRI scans. The most rapid progression rates to dementia among aMCI subjects (n = 224), over a 24-month period, were in those with NeoC-1 regional impairment (68.2%), followed by the Limbic regional impairment (48.8%). The same pattern of results was observed when only aMCI amyloid positive subjects were examined. Conclusions: The neuroimaging results closely parallel findings described recently among AD patients with the hippocampal sparing and limbic subtypes of AD neuropathology at autopsy. We conclude that NeoC-1, Limbic and other patterns of MRI atrophy may be useful markers for predicting the rate of progression of aMCI to AD and could have utility selecting individuals at higher risk for progression in clinical trials.
