Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury

Macrophages play an important role in peripheral nerve regeneration,but the specific mechanism of regeneration is still unclear.Our preliminary findings indicated that neutrophil peptide 1 is an innate immune peptide closely involved in peripheral nerve regeneration.However,the mechanism by which neutrophil peptide 1 enhances nerve regeneration remains unclear.This study was designed to investigate the relationship between neutrophil peptide 1 and macrophages in vivo and in vitro in peripheral nerve crush injury.The functions of RAW 264.7 cells we re elucidated by Cell Counting Kit-8 assay,flow cytometry,migration assays,phagocytosis assays,immunohistochemistry and enzyme-linked immunosorbent assay.Axonal debris phagocytosis was observed using the CUBIC(Clear,Unobstructed Brain/Body Imaging Cocktails and Computational analysis)optical clearing technique during Wallerian degeneration.Macrophage inflammatory factor expression in different polarization states was detected using a protein chip.The results showed that neutrophil peptide 1 promoted the prolife ration,migration and phagocytosis of macrophages,and CD206 expression on the surfa ce of macrophages,indicating M2 polarization.The axonal debris clearance rate during Wallerian degeneration was enhanced after neutrophil peptide 1 intervention.Neutrophil peptide 1 also downregulated inflammatory factors interleukin-1α,-6,-12,and tumor necrosis factor-αin invo and in vitro.Thus,the results suggest that neutrophil peptide 1 activates macrophages and accelerates Wallerian degeneration,which may be one mechanism by which neutrophil peptide 1 enhances peripheral nerve regeneration.

The Doppler effect induced by earthquakes:A case study of the Wenchuan MS8.0 earthquake

Seismologists have found that the first arrival frequencies of P waves at different seismic stations have different widths,that is,different periods or frequencies,and they think that this phenomenon can be used to identify whether a Doppler effect is induced by earthquakes.However,the fault rupture process of a real earthquake is so complex that it is difficult to identify a frequency shift similar to the Doppler effect.A method to identify whether a Doppler effect is induced by an earthquake is proposed here.If a seismic station is in the direction of fault rupture propagation,this station could observe a Doppler effect induced by the earthquake.The Doppler effect causes the frequency of the seismic wave to shift from low frequency to high frequency,and the high frequency amplitudes become mutually superimposed.Under the combined influences of the absorption effect,geometric spreading effect and Doppler effect,the high frequency amplitude of the seismic wave will gradually become higher than the low frequency amplitude with increasing epicentral distance.If we find that the high frequency amplitude is higher than the low frequency amplitude with increasing epicentral distance in the direction of fault rupture propagation,then there is a Doppler effect.The fault that generated the Wenchuan earthquake is a reverse fault,and its horizontal rupture propagation velocity was low.To link fault rupture propagation velocity with the Doppler effect and identify the Doppler effect more easily,we decompose three-component records into two directions:the direction of fault rupture propagation and the direction perpendicular to the fault rupture propagation along the fault plane.The initial components of the two directions are processed by wavelet transform.Several seismic stations in the direction of fault rupture propagation of the Wenchuan earthquake were selected,and it was found that with increasing epicentral distance,the high frequency amplitudes of the wavelet spectra become obviously higher than the low frequency amplitudes.It can be concluded that due to the existence of the Doppler effect,high frequency amplitudes can overcome the influences of the absorption and geometric spreading effects on seismic waves in the fault rupture propagation process.