Microstructure of flow pattern defects in boron-doped Czochralski-grown silicon

The morphology and microstructure of flow pattern defects （FPDs） in lightly boron-doped Czochralski-grown silicon （Cz-Si） crystals were investigated using optical microscopy and atomic force microscopy. The experimental results showed that the morphology of FPDs was parabola-like with several steps. Single-type and dual-type voids were found on the tip of FPDs and two heaves exist on the left and right sides of the void. All the results have proved that FPDs were void-type defects. These results are very useful to investigate FPDs in Cz-Si wafers further and explain the annihilation of FPDs during high-temperature annealing.

Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice

We sought to construct the adenoviral vector carrying the gene encoding mouse telomerase reverse transcriptase（mTERT）,as well as detect its expression and effect on the proliferation of neuronal stem cells.mTERT was am-plified by RT-PCR and then the eukaryotic expression vector of pDC-EGFP-TERT was constructed.After DNA sequence analysis,we detected that there were 293 cells transfected with pDC-EGFP-TERT and helper adenovirus plasmid pBHG lox ΔE1,and three Cre using Lipofectamine 2000 mediation,named Ad-mTERT-GFP,to pack-age adenoviral particles.The Ad-mTERT-GFP was used to infect neuronal stem cells and then the expression and activity of mTERT were detected.In addition,Bromodeoxyuridine labeling test identified the impact of mTERT overexpression on proliferation of neuronal stem cells.The recombinant adenoviral vector confirmed that mTERT was successfully constructed.Overexpression of mTERT stimulated the proliferation of neuronal stem cells both in vitro and in vivo.mTERT overexpression via adenoviral vector carrying mTERT cDNA upregulated the ability of proliferation in neuronal stem cells.

Doubt about antidepressant-like effect

Dear Editor： Extensive studies have measured antidepressant- like effects of chemical compounds to discover new drugs for depression treatment or to clarify the mo- lecular mechanisms of depression. Most useful anti- depressants including tricyclics, selective serotonin reuptake inhibitors （SSRIs）, noradrenaline reuptake inhibitors （NRIs）, serotonin and noradrenaline re- uptake inhibitors （SNRIs）, and monoamine oxidase inhibitors （MAOIs） exert antidepressant-like effects in forced swimmin~ test （FST） in mice and rats[1].

Current Situation of Land Use in Three-gorges Reservoir Region in 2010

Land is rare natural resource.Production and construction of all sectors in a region must be based on land.Thus,overall research and analysis on current situation of land use can reveal scope,depth and reasonableness of land use,is helpful for analyzing internal mechanism land use change,and can reflect production scale,level and characteristics,and also can provide basis for optimization and adjustment of land use structure.Based on RS and GIS technologies,with the aid of TM image data of the Three-gorges Reservoir Region in 2010,the data of current situation of land use in Three-gorges Reservoir Region was obtained,and current land use situation was analyzed using geographimetrics and landscape ecology methods.Results show that since natural,social and economic conditions are different,land type diversity,combination type and location index of counties in the Three-gorges Reservoir Region are varied.In the land use diversity index,Xingshan County has the most single land use type(mainly forest land); in land use degree,Yuzhong District has the highest land use degree; in the integrated index of land use,Changshou District has the lowest integrated index of land use.This study is expected to provide reference and basis for formulating policies of protecting ecological environment of the Three-gorges Reservoir Region.

Optimization of Training Program for Land Resource Management Major under the Background of Urban and Rural Integration

With constant deepening of development outlook of urban and rural integration,education circle starts to attach great importance to reform of personnel training program.Since scarcity of land resource and key role of land management in urban and rural integration,it is urgent to optimize the personnel training program for land resource management major.From aspects of optimum objectives,principles,program and implementation effect,this paper discusses training program for land resource management major under the background of urban and rural integration.It is expected to provide reference for cultivating land resource management personnel suitable for development of urban and rural integration.

Bioactive materials from berberine-treated human bone marrow mesenchymal stem cells promote alveolar bone regeneration by regulating macrophage polarization

Alveolar bone regeneration has been strongly linked to macrophage polarization.M1 macrophages aggravate alveolar bone loss,whereas M2 macrophages reverse this process.Berberine(BBR),a natural alkaloid isolated and refined from Chinese medicinal plants,has shown therapeutic effects in treating metabolic disorders.In this study,we first discovered that culture supernatant(CS)collected from BBR-treated human bone marrow mesenchymal stem cells(HBMSCs)ameliorated periodontal alveolar bone loss.CS from the BBR-treated HBMSCs contained bioactive materials that suppressed the M1 polarization and induced the M2 polarization of macrophages in vivo and in vitro.To clarify the underlying mechanism,the bioactive materials were applied to different animal models.We discovered macrophage colony-stimulating factor(M-CSF),which regulates macrophage polarization and promotes bone formation,a key macromolecule in the CS.Injection of pure M-CSF attenuated experimental periodontal alveolar bone loss in rats.Colony-stimulating factor 1 receptor(CSF1R)inhibitor or anti-human M-CSF(M-CSF neutralizing antibody,Nab)abolished the therapeutic effects of the CS of BBR-treated HBMSCs.Moreover,AKT phosphorylation in macrophages was activated by the CS,and the AKT activator reversed the negative effect of the CSF1R inhibitor or Nab.These results suggest that the CS of BBR-treated HBMSCs modulates macrophage polarization via the M-CSF/AKT axis.Further studies also showed that CS of BBR-treated HBMSCs accelerated bone formation and M2 polarization in rat teeth extraction sockets.Overall,our findings established an essential role of BBR-treated HBMSCs CS and this might be the first report to show that the products of BBR-treated HBMSCs have active effects on alveolar bone regeneration.