目的:探讨MUTYH基因AluYb8MUTYH位点(rs10527342)多态性与我国汉族人群原发性痛风发病风险的关系,为痛风的早期诊断筛选合适的遗传标记。方法:以2014年至2016年在川北医学院附属医院风湿血液科就诊的原发性痛风患者共183例作为实验组,...目的:探讨MUTYH基因AluYb8MUTYH位点(rs10527342)多态性与我国汉族人群原发性痛风发病风险的关系,为痛风的早期诊断筛选合适的遗传标记。方法:以2014年至2016年在川北医学院附属医院风湿血液科就诊的原发性痛风患者共183例作为实验组,同期健康体检者213名作为对照组,均收集外周血、临床和实验室资料,提取基因组DNA,采用聚合酶链反应(polymerase Chain Reaction,PCR)法检测DNA糖基化酶MUTYH基因AluYb8MUTYH插入/缺失(absence of insertion/presence of insertion,A/P)多态性在标本中的分布情况,并用统计软件SPSS16.0对结果进行分析。结果:MUTYH基因AluYb8MUTYH多态性在痛风组中有167例(91.26%)、对照组中有209例(98.12%)得到有效扩增;对照组中MUTYH基因AluYb8MUTYH多态性基因型分布符合Hardy-Wernberg平衡(χ~2=0.237,P=0.626);其中痛风组中A/A、A/P、P/P基因型频率分别为36.53%、49.70%和13.77%,对照组中A/A、A/P、P/P基因型频率分别为24.88%、51.67%和23.45%;痛风组各基因型与对照组进行比较,差异有统计学意义(χ~2=8.792,P=0.012),痛风组等位基因频率与对照组比较,差异有统计学意义(χ~2=8.540,P=0.003),其中A等位基因与痛风发病风险有关[OR=1.544,95%CI(1.153～2.088)]。结论:MUTYH基因AluYb8缺失型(A等位基因)可能增加痛风的易感性。展开更多
In this paper, we research on the higher education management mode of China and America based on the perspective of operation mechanism, separation of school and the government. As the thought of higher education mana...In this paper, we research on the higher education management mode of China and America based on the perspective of operation mechanism, separation of school and the government. As the thought of higher education management theory or a form, we jump out of the labyrinth of modernism manages view and the introduction of totally different from the modernist paradigm, from the angle of the theory research of postmodernism manages view. The paper is organized as the follows. In the first section, we generally introduce the basic concepts of higher education management and the relationship of the schools and government. Then, by comparing the pattern of the Chinese and the American, we analyze the difference in detail. Finally, we set up the prospect and the conclusion. In conclusion, our method combines the advantages of the American higher education management model which will be meaningful.展开更多
Hepatitis C virus(HCV)is a globally widespread ribonucleic acid virus that transmits through blood and sexual contact.Its morbidity and mortality are particularly higher in economically underdeveloped areas.Therefore,...Hepatitis C virus(HCV)is a globally widespread ribonucleic acid virus that transmits through blood and sexual contact.Its morbidity and mortality are particularly higher in economically underdeveloped areas.Therefore,an economical and effective diagnostic method for detection of HCV is urgently needed.In this study,we evaluated the diagnostic accuracy of the SD BIOLINE rapid diagnostic test for HCV detection.We searched for studies related to SD BIOLINE and HCV in PubMed,Embase,Web of Science,and the Cochrane Library and then designed inclusion and exclusion criteria.After extracting valid data,the included literature was evaluated with the quality assessment tool Quality Assessment of Diagnostic Accuracy Studies.After our data analysis,the sensitivity,specificity,positive likelihood ratio,negative likelihood ratio,diagnostic accuracy,summary receiver operating characteristic curve,funnel plot,box plot,and Fagan plot of the diagnosticmethod were determined.Nine articles with nine sets of data were finally included.The sensitivity and specificity were 0.94 and 0.98,respectively,the positive likelihood ratio was 79.53,the negative likelihood ratio was 0.05,the diagnostic odds ratio was 1590.32,and the summary receiver operating characteristic curve was 0.9958.The SD BIOLINE test has the advantages of high sensitivity,high specificity,low cost,and easy operation for diagnosing HCV.Therefore,we recommend using SD BIOLINE for rapid and effective screening of HCV,which is especially applicable for economically underdeveloped areas.展开更多
基金Project(CX20220097)supported by the Hunan Graduate Research Innovation Project,ChinaProject(U19A2099)supported by the National Natural Science Foundation of ChinaProject(6142906200108)supported by the Fund of Aerospace Research Institute of Material and Processing Technology,China。
文摘目的:探讨MUTYH基因AluYb8MUTYH位点(rs10527342)多态性与我国汉族人群原发性痛风发病风险的关系,为痛风的早期诊断筛选合适的遗传标记。方法:以2014年至2016年在川北医学院附属医院风湿血液科就诊的原发性痛风患者共183例作为实验组,同期健康体检者213名作为对照组,均收集外周血、临床和实验室资料,提取基因组DNA,采用聚合酶链反应(polymerase Chain Reaction,PCR)法检测DNA糖基化酶MUTYH基因AluYb8MUTYH插入/缺失(absence of insertion/presence of insertion,A/P)多态性在标本中的分布情况,并用统计软件SPSS16.0对结果进行分析。结果:MUTYH基因AluYb8MUTYH多态性在痛风组中有167例(91.26%)、对照组中有209例(98.12%)得到有效扩增;对照组中MUTYH基因AluYb8MUTYH多态性基因型分布符合Hardy-Wernberg平衡(χ~2=0.237,P=0.626);其中痛风组中A/A、A/P、P/P基因型频率分别为36.53%、49.70%和13.77%,对照组中A/A、A/P、P/P基因型频率分别为24.88%、51.67%和23.45%;痛风组各基因型与对照组进行比较,差异有统计学意义(χ~2=8.792,P=0.012),痛风组等位基因频率与对照组比较,差异有统计学意义(χ~2=8.540,P=0.003),其中A等位基因与痛风发病风险有关[OR=1.544,95%CI(1.153～2.088)]。结论:MUTYH基因AluYb8缺失型(A等位基因)可能增加痛风的易感性。
文摘In this paper, we research on the higher education management mode of China and America based on the perspective of operation mechanism, separation of school and the government. As the thought of higher education management theory or a form, we jump out of the labyrinth of modernism manages view and the introduction of totally different from the modernist paradigm, from the angle of the theory research of postmodernism manages view. The paper is organized as the follows. In the first section, we generally introduce the basic concepts of higher education management and the relationship of the schools and government. Then, by comparing the pattern of the Chinese and the American, we analyze the difference in detail. Finally, we set up the prospect and the conclusion. In conclusion, our method combines the advantages of the American higher education management model which will be meaningful.
文摘Hepatitis C virus(HCV)is a globally widespread ribonucleic acid virus that transmits through blood and sexual contact.Its morbidity and mortality are particularly higher in economically underdeveloped areas.Therefore,an economical and effective diagnostic method for detection of HCV is urgently needed.In this study,we evaluated the diagnostic accuracy of the SD BIOLINE rapid diagnostic test for HCV detection.We searched for studies related to SD BIOLINE and HCV in PubMed,Embase,Web of Science,and the Cochrane Library and then designed inclusion and exclusion criteria.After extracting valid data,the included literature was evaluated with the quality assessment tool Quality Assessment of Diagnostic Accuracy Studies.After our data analysis,the sensitivity,specificity,positive likelihood ratio,negative likelihood ratio,diagnostic accuracy,summary receiver operating characteristic curve,funnel plot,box plot,and Fagan plot of the diagnosticmethod were determined.Nine articles with nine sets of data were finally included.The sensitivity and specificity were 0.94 and 0.98,respectively,the positive likelihood ratio was 79.53,the negative likelihood ratio was 0.05,the diagnostic odds ratio was 1590.32,and the summary receiver operating characteristic curve was 0.9958.The SD BIOLINE test has the advantages of high sensitivity,high specificity,low cost,and easy operation for diagnosing HCV.Therefore,we recommend using SD BIOLINE for rapid and effective screening of HCV,which is especially applicable for economically underdeveloped areas.
