Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbo...Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.展开更多
Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules b...Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules by wrapping morphogenesis protein bones-2(BMP-2)using chitosan nanoparticles,and compositing collagen materials.Methods:Twenty four SpragueDawley rats were randomly divided into four groups with six rats in each group,that is Group A(control group),Group B(only treated with collagen),Group C(rhBMP-2+collagen treated group) and Group D(rhBMP-2/cs+collagen treated group).The composite materials for each group were implanted in the bilateral peroneal muscle pouches in rats.The peroneal muscles were only separated without implanting any materials in control group.Rats were sacrificed 2 weeks and 4 weeks post treatment and samples were cut off for general observation,Micro CT scans and histological observation.Results:General observation showed no new bone formation in Groups A and B mice,while new bones were formed in Groups C and D mice.Two weeks after treatment Micro CT scans showed that The bone volume fraction(BVF),trabecular thickness(Tb. Th),bone mineral density(BMD) in Group C mice were all higher than that in Group D(P<0.05). At the fourth week,the BVK,Tb.Th and BMD were significantly higher than that at the second week(P<0.01).Conclusions:The slow-release effect of rhBMP-2/cs sustained-release capsules can significantly promote ectopic osteogenesis.Its bone formation effect is better than that of rhBMP-2 burst-release group.展开更多
基金Supported by the Research and Development Project of Scientific and Technological Industry of Guangdong Province(2011B080701053)
文摘Objective:To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing. Methods:Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone.At the 1st,2nd,4th,6th,8th and 12th week after the operation, they were examined by X-ray and ultrasound,respectively.All detection results were scored according to a generally accepted standard.Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.Results:In each healing stage,the results of the ultrasonic inspection were basically consistent with those of the X-ray examination,as supported by a Spearman rank correlation coefficient of 0.892(P【0.001). Conclusions:Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.
基金supported by Guangdong Province Science and Technology Foundation,Guangdong,China(No:2011B080701053)
文摘Objective:To explore the effect of sustained-release recombinant human bone morphogenetic protein-2(rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules by wrapping morphogenesis protein bones-2(BMP-2)using chitosan nanoparticles,and compositing collagen materials.Methods:Twenty four SpragueDawley rats were randomly divided into four groups with six rats in each group,that is Group A(control group),Group B(only treated with collagen),Group C(rhBMP-2+collagen treated group) and Group D(rhBMP-2/cs+collagen treated group).The composite materials for each group were implanted in the bilateral peroneal muscle pouches in rats.The peroneal muscles were only separated without implanting any materials in control group.Rats were sacrificed 2 weeks and 4 weeks post treatment and samples were cut off for general observation,Micro CT scans and histological observation.Results:General observation showed no new bone formation in Groups A and B mice,while new bones were formed in Groups C and D mice.Two weeks after treatment Micro CT scans showed that The bone volume fraction(BVF),trabecular thickness(Tb. Th),bone mineral density(BMD) in Group C mice were all higher than that in Group D(P<0.05). At the fourth week,the BVK,Tb.Th and BMD were significantly higher than that at the second week(P<0.01).Conclusions:The slow-release effect of rhBMP-2/cs sustained-release capsules can significantly promote ectopic osteogenesis.Its bone formation effect is better than that of rhBMP-2 burst-release group.
