教育、科技、人才一体布局与科学基金发展战略

教育、科技、人才一体布局的统筹部署已经成为全面建设社会主义现代化国家的基础性、战略性支撑,深刻理解与把握教育、科技、人才一体布局重大意义对开展基础研究资助至关重要。在厘清一体布局对基础研究资助新要求的前提下,明确基础研究资助在科学研究、科学教育与人才培养有机结合中的定位和作用,完善国家自然科学基金资助管理体系,探索落实一体布局的着力点,从而提出新时代科学基金发展的战略思路,为教育强国、科技强国、人才强国建设提供源头支撑。

近十年NSF资助率和资助强度上升对我国科学基金资助工作的启示

美国国家科学基金会(National Science Foundation,U.S.,NSF)2022财年预算为88.4亿美元,比2021财年拨款增长4.1%,创历史新高。NSF官网数据显示,资助率从2013年的22%稳步提高到2022年的28%,平均资助强度也从52.5万美元/项增至80.6万美元/项。统计数据显示,NSF资助率稳步上升可归因于每年申请量从近50000项逐渐下降至40000项左右,而资助项目量维持在11000项左右;平均资助强度的提高得益于NSF在维持资助项目量不变的同时,年度预算大幅提高,从约60亿美元增加至接近90亿美元。本文基于近十年NSF总体资助变化,提出对我国科学基金资助工作启示,认为我国应该持续加大基础研究投入以提高自然科学基金资助率,将科研人才红利转化为高质量发展的动力,在大国科技竞争中占据有利地位。

Molecular cytogenetic analyses of two new wheat-rye 6RL translocation lines with resistance to wheat powdery mildew

Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).

Effect of ammonium sulfate on the formation of zinc sulfide species on hemimorphite surface and its role in sulfidation flotation

Effectively strengthening the surface sulfidation is essential for recovering hemimorphite by froth flotation.In this work,inductively coupled plasma optical emission spectrometer(ICP-OES)measurements,Visual MINTEQ calculation,X-ray photoelectron spectroscopy(XPS)analysis,time of flight secondary ion mass spectrometry(ToF-SIMS)analysis,and micro-flotation experiments were explored to systematically investigate the effect of ammonium sulfate((NH_(4))_(2)SO_(4))on the formation of zinc sulfide species on hemimorphite surface and its role in sulfidation flotation.The results showed that(NH_(4))_(2)SO_(4)exhibited a positive influence on hemimorphite sulfidation flotation.It was ascribed to the number of zinc components in the form of Zn^(2+)and[Zn(NH_(3))_(i)]^(2+)(i=1–4)increased in the flotation system after hemimorphite treatment with(NH_(4))_(2)SO_(4),which was beneficial to its interaction with sulfur species in solution,resulting in a dense and stable zinc sulfide layer generated on the hemimorphite surface.[Zn(NH_(3))_(i)]^(2+)participated in the sulfidation reaction of hemimorphite as a transition state.In addition the sulfidation reaction of hemimorphite was accelerated by(NH_(4))_(2)SO_(4).Thus,(NH_(4))_(2)SO_(4)presents a vital role in promoting the sulfidation of hemimorphite.

Advances in Detection Methods of Koi Herpes Virus

Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently, virus isolation and identification is still the traditional method for the detection of Koi herpes virus, while molecular biology detection method has become the current developmental di- rection due to its characteristics of more sensitive, specific and rapid. Furthermore, people are still committed to exploring new detection methods for the detection of Koi herpes vires. In this paper, traditional and newly-developed detection methods of Koi herpes vires in recent years were summarized, in order to provide refer- ence for further exploring rapid and accurate diagnostic detection method.

Establishment and Application of the TaqMan Real-Time Fluorescence Quantitative PCR Detection Assay for Koi Herpes Virus

[ Objective ] This study aimed to establish a rapid and effective quarantine method of Koi herpes virus. [ Method] Primers and corresponding TaqMan probe were designed based on the conserved sequence of Koi herpes virus （KHV） pol-ymerase gene （Sph） to establish a rapid and effective fluorescence quantitative PCR method for Koi herpes virus detection. The cell cultures were detected by using the established fluorescence quantitative PCR assay, and the results were com- pared with that of conventional PCR. [ Result] The sensitivity of fluorescence quantitative PCR was higher than that of conventional PCR. The minimum copy num- ber that could be detected was 1.6 - 102 copies/p.1. The established method was adopted for sample detection, and a reliable diagnostic result could be obtained within 4 h. [Conclusion] The established method is rapid, sensitive, specific and repeatable, which is conducive to the rapid detection of Koi herpes virus. Key words Koi herpes virus; Fluorescence quantitative PCR; Detection

Microbial synthesis of anti-tumor agent oxysophoridine through one step by filamentous fungus

Sophoridine and oxysophoridine are alkaloids extracted from Chinese traditional medicinewith significant antitumor activities. Computer-aided design and preliminary structure-activity relationship studiesshowed that the biological activity of sophoridine can be improved after oxidation (oxysophoridine) whileits toxicity significantly decreased. A new method to prepare oxysophoridine from natural product Sophoridineby biotransformation was established for the first time except for traditional extraction from plants and chemicalsynthesis. Positive biocatalytic reaction was detected by TLC. Structure of conversion product was elucidatedbased on NMR spectroscopy. Optimizations of reactions including substrate concentration, pH, conversion timeand inoculation quantity were complemented which analyzed by LC-MS. Results showed that filamentous fungusXylariales. sp F005 (CCTCC M2014660) has the capacity of synthetizing oxysophoridine from sophoridinein the selected 37 strains. The yield of oxysophoridine reached highest at conditions pH 6.0, the third day ofconversion time, 5.0 mg substrate and 5% inoculums respectively. The method for preparing oxysophoridinecatalyzed by enzymes with green and sustainable synthetic processes was developed.

转化化学:医学驱动的化学前沿研究

本文围绕医学与化学的前沿交叉研究,基于第288期双清论坛的研讨内容,提出通过临床医学的问题和场景来启发化学的前沿研究,进而通过分子科学的创新推动医学问题的解决。这一研究思路有望推动“转化化学”的快速发展,促进化学和医学两个学科的协同创新。同时,本文还凝练了该领域的主要研究方向和若干关键科学问题,指出面临的技术瓶颈,思考未来5~10年的发展目标和资助重点。

双清论坛:一种典型学术研讨会议的组织管理实践与思考

双清论坛是国家自然科学基金委员会设立的战略性学术研讨会议,旨在探索科学前沿,凝练科学问题,促进学科交叉融合。双清论坛坚持百花齐放、百家争鸣的方针,引导科学家在思维碰撞中凝练科学问题,同时形成服务科学基金资助工作的共识建议,为科学基金乃至国家的科技政策提供咨询建议与政策支撑。本文分析当前国内外著名学术研讨会议的特点,总结双清论坛的建设实践经验,围绕新时期科学基金的新使命、新定位,提出应进一步发挥双清论坛凝聚科学家创新思想的功能作用,着力引导科技界重视凝练科学问题,推动科研范式变革,服务构建理念先进、制度规范、公正高效的新时代科学基金体系。

Specific pupylation as IDEntity reporter(SPIDER)for the identification of protein-biomolecule interactions

Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are available,highly robust and reliable methods are always desired.By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin(SA)-biotin system,we developed the Specific Pupylation as IDEntity Reporter(SPIDER)method for identifying protein-biomolecule interactions.Using SPIDER,we validated the interactions between the known binding proteins of protein,DNA,RNA,and small molecule.We successfully applied SPIDER to construct the global protein interactome for m^(6)A and m RNA,identified a variety of uncharacterized m^(6)A binding proteins,and validated SRSF7 as a potential m^(6)A reader.We globally identified the binding proteins for lenalidomide and Cob B.Moreover,we identified SARS-CoV-2-specific receptors on the cell membrane.Overall,SPIDER is powerful and highly accessible for the study of proteinbiomolecule interactions.

Long-term cattle manure application to saline-sodic soil increases maize yield by decreasing key obstacle factors in the black soil region of Northeastern China

Poor soil physical properties, serious salinization and low soil nutrients are the limiting factors for crop yield in saline-sodic soil. Long-term cattle manure application is an important measure that can affect the physicochemical properties and increase the maize yield of saline-sodic soil. This experiment included five treatments according to the history of cattle manure application: a control treatment with no cattle manure (CK) and treatments with cattle manure application for 14 years (14 a), 17 years (17 a), 20 years (20 a), and 25 years (25 a). The results indicated that compared with the CK treatment, long-term cattle manure application to saline-sodic soil resulted in significant increases in soil organic matter (SOM), soil total nitrogen (TN) and available nutrients at the 0-20 cm and 20-40 cm depths (p<0.05). The soil physical properties improved significantly, and cattle manure application significantly decreased the soil bulk density (ρb) and soil density (ρd) and increased the soil total porosity (ft) and water-holding capacity (WHC). With the number of years of cattle manure application, the soil pH, electrical conductivity (EC), exchangeable sodium percentage (ESP) and sodium adsorption ratio (SAR1:5) decreased significantly, and the maize yield gradually increased over time from 8690 kg/hm2 in the CK treatment to 14 690 kg/hm2 in the 25a treatment. There were significant differences among all treatments (p<0.05). The results showed that long-term cattle manure application decreased the soil ρb and saline-alkaline properties, which was the main factor that affected the maize yield in the saline-sodic soil, especially for soil ρb.

浅论科学基金资助管理工作的五大关系——基于对科学基金深化改革理念与实践的思考

本文基于对科学基金深化改革理念与实践的思考,归纳并探讨了科学基金资助管理工作的五大关系,包括自由探索与需求牵引、全面部署与重点支持、人才培养与科研生态、经费投入与科学管理、自主创新与开放合作,以期能够从更多元的维度来帮助读者理解改革之初心及其历程。

A quasi-3D Fano resonance cavity on optical fiber end-facet for high signal-to-noise ratio dip-and-read surface plasmon sensing

Surface plasmon devices mounted at the end-facets of optical fibers are appealing candidates for rapid and point-of-care sensing applications,by offering a special dip-and-read operation mode.At present,these devices’noise-equivalent limits-of-detection lag far behind the free-space counterparts,leaving them incapable of most biosensing applications.Here we report a quasi-3D Fano resonance cavity and its fabrication method to fundamentally improve the quality factor and coupling efficiency for fiber-coupled surface plasmon resonance.In this device,the Fano resonance combines the high coupling efficiency of a Fabry-Pérot etalon and the high quality factor resonance of a plasmonic crystal cavity.The quasi-3D device was fabricated on a planar substrate and transferred to a single-mode fiber end-facet,which requires a low-adhesion yet surface-plasmon-tunneling interface between the device and the planar substrate.Such an interface was realized with a nanocap-slit unit structure,of which the plasmonic crystal was consisted.A noise-equivalent limit of detection of~10-7 RIU was experimentally obtained,allowing bovine serum albumin physical adsorption to be distinguished at ng mL-1 level concentrations.Therefore,breaking through the long-standing signal-to-noise ratio bottleneck,this work makes fiber end-facet surface plasmon devices into one of high sensitivity label-free sensing technologies.At the same time,it provides an enabling top-down fabrication technology for making 3D plasmonic structures on fiber end-facets at the nanometer scale.

Distribution and stability of water-stable aggregates as affected by long-term cattle manure application to saline-sodic soil in the black soil region of northeastern China

Saline-sodic soil has a poor structure,low nutrient content,and excessive sodium in the western Heilongjiang Province,resulting in low crop productivity.Experimental treatments were established by applying manure to the soil for 5 years,12 years,and 16 years and soil without manure application was used as a control treatment(CK).The results indicate that the application of manure significantly increased soil macroaggregates,the mean weight diameter(MWD)and the geometric mean diameter(GMD)compared to those for the CK treatment.The soil organic matter(SOM)concentration increased from 17.8 to 47.9 g/kg,the soil pH decreased from 10.18 to 7.89,and the electrical conductivity(EC)decreased from 4.92 to 0.19 dS/m.The soil exchangeable Na^(+)was decreased and exchangeable Ca^(2+)was increased in the treatments with manure application compared with the CK treatment.And a decrease in the CaCO_(3)content was observed in the treatment with manure.Water-stable aggregates(WSAs)of greater than 2.0 mm were the dominant factor driving the changes in the MWD,and WSAs of 1.0-2.0 mm were the dominant factor driving the changes in the GMD.The correlation matrix showed that the SOM and soil exchangeable Ca^(2+)concentration was positively correlated with the stability of the WSAs,while the pH,EC,and soil exchangeable Na^(+)were negatively and significantly correlated.We conclude that the long-term application of manure to saline-sodic soil can increase the proportion of soil macroaggregates and thus increase the stability of WSAs,as a result of the formation of soil macroaggregates mainly caused by the increase in the organic colloidal matter and soil exchangeable Ca^(2+),and by the decrease in soil exchangeable Na^(+).

论罪犯改造客体

罪犯改造客体是复合客体,具有对改造实践活动的高度依赖性,与罪犯改造主体相互作用,具有彻底改造性,具有改造的复杂性。罪犯改造客体的基本内容包括不思悔改的服刑态度、不认罪不悔罪的改造态度、导致犯罪的个体因素、人格品质欠缺或变异、致罪因素残余与社会适应能力欠缺,其中不思悔改的服刑态度处于表层,罪犯人格品质欠缺处于核心,其罪犯个性倾向性欠缺则处于核心的中心,而罪犯社会认知能力欠缺则分散在各个层次之中。罪犯改造客体具有需要改造性、隐蔽性、不易改造性、可以改造性、主观能动性等特性。