Breeding of Anti-diarrhea Gene in Local and Exotic Pig Breeds in Guizhou Province

[Objectives]This study was conducted to breed special pig breeds resistant to diarrhea by using modern biotechnology.[Methods]From Guizhou local breeds,such as Nuogu pigs,Kele pig,Yorkshire pigs and Duroc pigs,190 samples were collected for the analysis of anti-diarrhea gene.[Results]The anti-diarrhea genotype frequency of Kele pigs was 70.00%,which was higher than that of Nuogu pigs(67.37%)and Yorkshire pigs(Yorkshire pigs and Duroc pigs)(50.59%).The favorable anti-diarrhea gene of all Nuogu pigs,Kele pigs,and Yorkshire pigs and Duroc pigs was G,with gene frequencies of 0.7355,0.8368 and 0.8500,respectively,and the frequencies of allele A were 0.2645,0.1632 and 0.1500,respectively.In the process of generation selection,combination selection of GG♂×GG♀,GG♂×GA♀,GA♂×GG♀and GA♂×GA♀was conducted,and GG individuals were selected while gradually phasing out GA and AA individuals.The anti-diarrhea genotypes of 98 pigs in the offspring were tested,and it was found that the frequency of genotype GG was greatly improved,and the frequencies in Nuogu pigs,Kele pigs,Yorkshire pigs and Duroc pigs were increased to 73.91%,81.82%,85.25%and 66.67%respectively,thus forming a special anti-diarrhea breed.[Conclusions]This study provides a basis for selecting excellent breeding pigs,establishing core populations and screening resistance genes in the core populations and their offspring.

Analysis and Utilization of Straw-preserved Frozen Semen from Native and Imported Pig Breeds in Guizhou Province

[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.

AR Model Based on Time Series Modeling for Predicting Egg Market Price in 2021

Eggs,as a meat consumer product in China,are closely related to the vegetable basket project.Exploring and predicting the future trend of egg market price is of great significance for stabilizing egg price and market supply.In this study,the time series AR model was used for fitting the egg market prices in the 66 d from January 1 to March 7,2021,and the delay operator nlag18 was used for white noise test,giving pr>probability of chisq<0.005.The time series was not a white noise series,and then the stationary series was used for modeling.The optimal model was selected as the AR series(BIC(3,0)),and finally,the egg market price model AM was obtained as X_(t)=9.0556+(1+0.8926)ε_(t),which was the optimal model.The model showed that the egg price fluctuations in 2021 will be clustered,and the later price will be significantly affected by external factors in the previous period.The dynamic prediction results of the model showed that the egg price would stop falling in March 2020,and the egg price would continue to slow down in March.

Thinking on Breeding of Fecundity Genes in Guizhou Black Goats Through Cost-benefit Analysis of Mutton Sheep by SAS Multivariate Stepwise Regression

The total output value of mutton in Northwestern China has accounted for more than 60%of the total output value of animal husbandry over the years.It can be seen that the mutton industry in Northwest China not only plays a pivotal role in animal husbandry,but also plays an important role in Chinese agriculture.In this study,based on cost accounting theory,income-related theories and total factor productivity theory,using basic knowledge of statistics and economics,drawing on existing research results at home and abroad,and adopting a combination of qualitative analysis and quantitative analysis of SAS multiple stepwise regression,the changing trends of cost-benefit of mutton sheep breeding in Northwest agricultural and pastoral areas and influencing factors of production costs and production efficiency were investigated,aiming to provide reference for saving mutton sheep feeding material resources,reducing mutton sheep breeding costs,and improving mutton sheep breeding benefits.