Green tea catechin, (–)-epigallocatechin-3-gallate [(–)-EGCG], was found to increase osteogenic functioning in mesenchymal stem cells. This study qualified EGCG, the strongest inhibitory efficiency for receptor acti...Green tea catechin, (–)-epigallocatechin-3-gallate [(–)-EGCG], was found to increase osteogenic functioning in mesenchymal stem cells. This study qualified EGCG, the strongest inhibitory efficiency for receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-activated osteoclastogenesis among other green tea catechins for RAW264, a murine preosteoclast cell line. Moreover, EGCG inhibited tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cell formation dose dependently in both single culture and co-culture systems, the expression of transcription factor, nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and some osteoclastic genes. Especially, EGCG exhibited a strong inhibitory effect on the expression levels of RANK, the receptor of RANKL, and OSCAR, a key co-stimulator of the RANKL/RANK signal. Simultaneously, apoptotic genes expression and Hoechst staining revealed that EGCG induced apoptosis in RAW264. Taken together, these data suggest that the inhibitory effect of EGCG to osteoclastogenesis is associated with a down regulation of RANKL/RANK signal, and increased apoptosis of preosteoclasts.展开更多
Rechargeable neutral aqueous zinc-air batteries(ZABs)are a promising type of energy storage device with longer operating life and less corrosiveness compared with conventional alkaline ZABs.However,the neutral ZABs no...Rechargeable neutral aqueous zinc-air batteries(ZABs)are a promising type of energy storage device with longer operating life and less corrosiveness compared with conventional alkaline ZABs.However,the neutral ZABs normally possess poor oxygen evolution reactions(OERs)and oxygen reduction reactions performance,resulting in a large charge–discharge voltage gap and low round-trip efficiency.Herein,we demonstrate a sunlight-assisted strategy for achieving an ultralow voltage gap of 0.05 V in neutral ZABs by using the FeOOH-decorated BiVO4(Fe-BiVO4)as an oxygen catalyst.Under sunlight,the electrons move from the valence band(VB)of Fe-BiVO_(4) to the conduction band producing holes in VB to promote the OER process and hence reduce the overpotential.Meanwhile,the photopotential generated by the Fe-BiVO_(4) compensates a part of the charging potential of neutral ZABs.Accordingly,the energy loss of the battery could be compensated via solar energy,leading to a record-low gap of 0.05 V between the charge and discharge voltage with a high round-trip efficiency of 94%.This work offers a simple but efficient pathway for solar-energy utilization in storage devices,further guiding the design of high energy efficiency of neutral aqueous ZABs.展开更多
丝素蛋白是一种从蚕丝中提取的天然高分子纤维蛋白,有很好的抗氧化和减少光损伤的作用。为了研究丝素蛋白对皮肤光损伤的保护作用,以UVB诱导人角质形成细胞(HaCaT)损伤为模型,通过ELISA和荧光检测细胞内活性氧(ROS)和钙离子(Ca^(2+))的...丝素蛋白是一种从蚕丝中提取的天然高分子纤维蛋白,有很好的抗氧化和减少光损伤的作用。为了研究丝素蛋白对皮肤光损伤的保护作用,以UVB诱导人角质形成细胞(HaCaT)损伤为模型,通过ELISA和荧光检测细胞内活性氧(ROS)和钙离子(Ca^(2+))的水平,采用免疫荧光检测瞬时受体电位阳离子通道亚家族V成员1(TRPV1)和Claudin-1的表达。此外,还使用蛋白质印迹分析了紧密连接相关蛋白Occludin和Claudin-1的表达。结果表明,120 m J/cm^(2)的UVB刺激显著降低细胞活力,而100μg/m L丝素蛋白的存在显著增加细胞活力。UVB刺激导致细胞内活性氧水平升高,激活TRPV1通道,诱导细胞内Ca^(2+)水平增加。同时炎症因子白细胞介素1α(IL-1α)(P<0.01)和S100钙结合蛋白A8(S100A8)(P<0.05)水平也显著升高,引发炎症反应。添加丝素蛋白的HaCaT细胞受到UVB的影响则显著降低(P<0.05)。实验证明,UVB会破坏屏障蛋白,导致屏障蛋白Claudin-1和Occludin的表达下降。丝素蛋白的加入可以降低这种影响,保护细胞间紧密连接。因此,丝素蛋白可能具有降低氧化应激、屏障损伤和炎症发生,保护皮肤免受UVB诱导的光损伤的潜在能力。展开更多
Activated carbon was prepared from cattail by H3PO4 activation. The effects influencing the surface area of the resulting activated carbon followed the sequence of activated temperature 〉 activated time 〉 impregnati...Activated carbon was prepared from cattail by H3PO4 activation. The effects influencing the surface area of the resulting activated carbon followed the sequence of activated temperature 〉 activated time 〉 impregnation ratio 〉 impregnation time. The optimum condition was found at an impregnation ratio of 2.5, an impregnation time of 9 hr, an activated temperature of 500℃, and an activated time of 80 min. The Brunauer-Emmett-Teller surface area and average pore size of the activated carbon were 1279 m^2/g and 5.585 nm, respectively. A heterogeneous structure in terms of both size and shape was highly developed and widely distributed on the carbon surface. Some groups containing oxygen and phosphorus were formed, and the carboxyl group was the major oxygen-containing functional group. An isotherm equilibrium study was carried out to investigate the adsorption capacity of the activated carbon. The data fit the Langmuir isotherm equation, with maximum monolayer adsorption capacities of 192.30 mg/g for Neutral Red and 196.08 mg/g for Malachite Green. Dye-exhausted carbon could be regenerated effectively by thermal treatment. The results indicated that cattail-derived activated carbon was a promising adsorbent for the removal of cationic dyes from aqueous solutions.展开更多
AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transf...AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy,flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay.Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice,and the pathological analysis of tumor formation was performed.Two cytoskeletal proteins were detected by Western blotting. RESULTS:Compared with HepG2 cells,HepG2.2.15 cells showed organelle degeneration and filopodia disappearance under electron microscope.HepG2.2.15 cells proliferated and migrated slowly in vitro,and hardly formed tumor and lung metastasis in nude mice.Flow cytometry showed that the majority of HepG2.2.15 cells were arrested in G1 phase,and apoptosis was minor in both cell lines.Furthermore,the levels of cytoskeletal proteins F-actin and Ezrin were decreased in HepG2.2.15 cells. CONCLUSION:HepG2.2.15 cells demonstrated a lower proliferation and invasion ability than the HepG2 cells due to HBV transfection.展开更多
Soy consumption has been associated with potential health benefits in reducing chronic diseases.These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides.Thus,more studi...Soy consumption has been associated with potential health benefits in reducing chronic diseases.These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides.Thus,more studies are required to identify these bioactive peptides,and elucidate their biological mechanisms of action.In the present study,a novel peptide iglycin was purifi ed from soybean seeds with a molecular mass of 3.88 k Da.Thereafter,iglycin reduced fasting blood glucose and restored insulin sensitivity of C57 BL/6 J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1(IRS1)and AKT in adipose tissue.Furthermore,it improved glucose uptake,induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition.In addition,it decreased reactive oxygen species production,lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity,mitochondrial membrane potential and intracellular ATP store.These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling,which was associated with inhibition of oxidative stress,adipocyte apoptosis,and improvement of mitochondrial function.展开更多
Besides its ecological services to China and even Asia,the Qingzang Plateau(QP)hosts a rich variety of wildlife species.During the last century,wildlife population decreased quickly on the QP,driven by human intervent...Besides its ecological services to China and even Asia,the Qingzang Plateau(QP)hosts a rich variety of wildlife species.During the last century,wildlife population decreased quickly on the QP,driven by human interventions.Recently,wildlife has witnessed rapid recovery mainly propelled by a series of wildlife conservation policies.However,some cautions merit attentions to sustain wildlife restoration and conservation on the QP.This paper casted an overview of environmental and social-economic changes on the QP affecting wildlife subsistence.Re-sults show that QP has been warming,which can benefit wildlife recovery by easing extreme low temperature stresses.The fast growing social economy across the QP lays a solid economic foundation for investing on wildlife protection.Measures such as establishing conservation areas,constructing wildlife pathway corridors,and en-couraging herdsman moving out from wildlife rich regions,have boosted wildlife recovery.However,wildlife recovery is constrained by the limited carrying capacity of the ecosystem,left by domestic livestock.Additionally,fences intended to delineate conservation areas or to separate each type of grassland use,have brought about profound side effects on wildlife through fragmentation of their habitats.It is recommended to set up the fence in a more ecological way,which can be achieved by bypassing the wildlife frequent pathway and using mate-rials devoid of steel barb.Only considering both opportunities and problems simultaneously,can the wildlife protection on the QP be sustained.展开更多
原发中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)是仅累及脑实质、脊髓、眼、颅神经及脑膜,无中枢神经(central nervous system,CNS)以外部位受累的一类结外非霍奇金淋巴瘤。目前,治疗主要是以大剂量甲氨蝶呤...原发中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)是仅累及脑实质、脊髓、眼、颅神经及脑膜,无中枢神经(central nervous system,CNS)以外部位受累的一类结外非霍奇金淋巴瘤。目前,治疗主要是以大剂量甲氨蝶呤为基础的化疗,联合大剂量化疗/自体干细胞移植(autologous stem cell transplantation,auto-HSCT)或减量的全脑放疗。但缓解率较低,不良反应较大。研究显示,10%~15%的患者对诱导化疗原发耐药,即使治疗有效,仍有50%的患者复发。近年来,PCNSL遗传学和分子的研究极大地促进了生物学机制的理解。小分子药物和靶向药物治疗的前景可观,有望减少不良反应的同时,提高患者缓解率、延长寿命。本文主要对目前PCNSL的新治疗方向进行综述。展开更多
文摘Green tea catechin, (–)-epigallocatechin-3-gallate [(–)-EGCG], was found to increase osteogenic functioning in mesenchymal stem cells. This study qualified EGCG, the strongest inhibitory efficiency for receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-activated osteoclastogenesis among other green tea catechins for RAW264, a murine preosteoclast cell line. Moreover, EGCG inhibited tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cell formation dose dependently in both single culture and co-culture systems, the expression of transcription factor, nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and some osteoclastic genes. Especially, EGCG exhibited a strong inhibitory effect on the expression levels of RANK, the receptor of RANKL, and OSCAR, a key co-stimulator of the RANKL/RANK signal. Simultaneously, apoptotic genes expression and Hoechst staining revealed that EGCG induced apoptosis in RAW264. Taken together, these data suggest that the inhibitory effect of EGCG to osteoclastogenesis is associated with a down regulation of RANKL/RANK signal, and increased apoptosis of preosteoclasts.
基金This work was supported by the National Natural Science Foundation of China(Grant No.22278349)Natural Science Foundation of Hebei Province(Grant No.B2023203026)+2 种基金Youth Foundation of Hebei Educational Committee(Grant No.QN2020137)Cultivation Project for Basic Research and Innovation of Yanshan University(Grant No.2021LGZD015)Subsidy for Hebei Key Laboratory of Applied Chemistry after Operation Performance(Grant No.22567616H).
文摘Rechargeable neutral aqueous zinc-air batteries(ZABs)are a promising type of energy storage device with longer operating life and less corrosiveness compared with conventional alkaline ZABs.However,the neutral ZABs normally possess poor oxygen evolution reactions(OERs)and oxygen reduction reactions performance,resulting in a large charge–discharge voltage gap and low round-trip efficiency.Herein,we demonstrate a sunlight-assisted strategy for achieving an ultralow voltage gap of 0.05 V in neutral ZABs by using the FeOOH-decorated BiVO4(Fe-BiVO4)as an oxygen catalyst.Under sunlight,the electrons move from the valence band(VB)of Fe-BiVO_(4) to the conduction band producing holes in VB to promote the OER process and hence reduce the overpotential.Meanwhile,the photopotential generated by the Fe-BiVO_(4) compensates a part of the charging potential of neutral ZABs.Accordingly,the energy loss of the battery could be compensated via solar energy,leading to a record-low gap of 0.05 V between the charge and discharge voltage with a high round-trip efficiency of 94%.This work offers a simple but efficient pathway for solar-energy utilization in storage devices,further guiding the design of high energy efficiency of neutral aqueous ZABs.
文摘丝素蛋白是一种从蚕丝中提取的天然高分子纤维蛋白,有很好的抗氧化和减少光损伤的作用。为了研究丝素蛋白对皮肤光损伤的保护作用,以UVB诱导人角质形成细胞(HaCaT)损伤为模型,通过ELISA和荧光检测细胞内活性氧(ROS)和钙离子(Ca^(2+))的水平,采用免疫荧光检测瞬时受体电位阳离子通道亚家族V成员1(TRPV1)和Claudin-1的表达。此外,还使用蛋白质印迹分析了紧密连接相关蛋白Occludin和Claudin-1的表达。结果表明,120 m J/cm^(2)的UVB刺激显著降低细胞活力,而100μg/m L丝素蛋白的存在显著增加细胞活力。UVB刺激导致细胞内活性氧水平升高,激活TRPV1通道,诱导细胞内Ca^(2+)水平增加。同时炎症因子白细胞介素1α(IL-1α)(P<0.01)和S100钙结合蛋白A8(S100A8)(P<0.05)水平也显著升高,引发炎症反应。添加丝素蛋白的HaCaT细胞受到UVB的影响则显著降低(P<0.05)。实验证明,UVB会破坏屏障蛋白,导致屏障蛋白Claudin-1和Occludin的表达下降。丝素蛋白的加入可以降低这种影响,保护细胞间紧密连接。因此,丝素蛋白可能具有降低氧化应激、屏障损伤和炎症发生,保护皮肤免受UVB诱导的光损伤的潜在能力。
基金supported by the National Key Technology R&D Program for the 11th Five-year Plan of China (No.2006BAC10B03)the National Natural Science Foundation of China-Japan Science and Technology Agency (NSFC-JST) Strategic Joint Research Project (No.50721140017)the National Natural Science Foundation of China (No.50508019)
文摘Activated carbon was prepared from cattail by H3PO4 activation. The effects influencing the surface area of the resulting activated carbon followed the sequence of activated temperature 〉 activated time 〉 impregnation ratio 〉 impregnation time. The optimum condition was found at an impregnation ratio of 2.5, an impregnation time of 9 hr, an activated temperature of 500℃, and an activated time of 80 min. The Brunauer-Emmett-Teller surface area and average pore size of the activated carbon were 1279 m^2/g and 5.585 nm, respectively. A heterogeneous structure in terms of both size and shape was highly developed and widely distributed on the carbon surface. Some groups containing oxygen and phosphorus were formed, and the carboxyl group was the major oxygen-containing functional group. An isotherm equilibrium study was carried out to investigate the adsorption capacity of the activated carbon. The data fit the Langmuir isotherm equation, with maximum monolayer adsorption capacities of 192.30 mg/g for Neutral Red and 196.08 mg/g for Malachite Green. Dye-exhausted carbon could be regenerated effectively by thermal treatment. The results indicated that cattail-derived activated carbon was a promising adsorbent for the removal of cationic dyes from aqueous solutions.
基金Supported by Graduate Innovation Foundation of Harbin Medical University No.HCXB2010010Key Technology Project of Heilongjiang Science and Technology Department,No.ZJY04-0102
文摘AIM:To investigate the biological features of hepatitis B virus(HBV)-transfected HepG2.2.15 cells. METHODS:The cell ultrastructure,cell cycle and apoptosis,and the abilities of proliferation and invasion of HBV-transfected HepG2.2.15 and the parent HepG2 cells were examined by electron microscopy,flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trans-well assay.Oncogenicity of the two cell lines was compared via subcutaneous injection and orthotopic injection or implantation in nude mice,and the pathological analysis of tumor formation was performed.Two cytoskeletal proteins were detected by Western blotting. RESULTS:Compared with HepG2 cells,HepG2.2.15 cells showed organelle degeneration and filopodia disappearance under electron microscope.HepG2.2.15 cells proliferated and migrated slowly in vitro,and hardly formed tumor and lung metastasis in nude mice.Flow cytometry showed that the majority of HepG2.2.15 cells were arrested in G1 phase,and apoptosis was minor in both cell lines.Furthermore,the levels of cytoskeletal proteins F-actin and Ezrin were decreased in HepG2.2.15 cells. CONCLUSION:HepG2.2.15 cells demonstrated a lower proliferation and invasion ability than the HepG2 cells due to HBV transfection.
基金jointly supported by the National Natural Science Foundation of China(31400794)Sichuan Province Science Fund s for Distinguished Young Scholar(2019JDJQ0017)+1 种基金Young and middle-aged Talents Project of the National People’s Commission(2799300127)Fundamental Research Funds for the Central Universities,Southwest Minzu University(2019XMJXPY08)。
文摘Soy consumption has been associated with potential health benefits in reducing chronic diseases.These physiological functions have been attributed to soy proteins or more commonly to bioactive peptides.Thus,more studies are required to identify these bioactive peptides,and elucidate their biological mechanisms of action.In the present study,a novel peptide iglycin was purifi ed from soybean seeds with a molecular mass of 3.88 k Da.Thereafter,iglycin reduced fasting blood glucose and restored insulin sensitivity of C57 BL/6 J mice on a high-fat diet with increased phosphorylation of insulin receptor substrate 1(IRS1)and AKT in adipose tissue.Furthermore,it improved glucose uptake,induced translocation of intracellular GLUT4 to plasma membrane and activation of insulin signaling in adipocytes under insulin-resistant condition.In addition,it decreased reactive oxygen species production,lipid peroxidation and inhibited adipocyte apoptosis with improved mitochondrial function as evidenced by up-regulation of succinate dehydrogenase activity,mitochondrial membrane potential and intracellular ATP store.These data suggested that iglycin ameliorated insulin resistance via activation of insulin signaling,which was associated with inhibition of oxidative stress,adipocyte apoptosis,and improvement of mitochondrial function.
基金supported by the National Key Research&Development Program(Grant No.2019YFA0607302)CNSF(Grant No.41725003).
文摘Besides its ecological services to China and even Asia,the Qingzang Plateau(QP)hosts a rich variety of wildlife species.During the last century,wildlife population decreased quickly on the QP,driven by human interventions.Recently,wildlife has witnessed rapid recovery mainly propelled by a series of wildlife conservation policies.However,some cautions merit attentions to sustain wildlife restoration and conservation on the QP.This paper casted an overview of environmental and social-economic changes on the QP affecting wildlife subsistence.Re-sults show that QP has been warming,which can benefit wildlife recovery by easing extreme low temperature stresses.The fast growing social economy across the QP lays a solid economic foundation for investing on wildlife protection.Measures such as establishing conservation areas,constructing wildlife pathway corridors,and en-couraging herdsman moving out from wildlife rich regions,have boosted wildlife recovery.However,wildlife recovery is constrained by the limited carrying capacity of the ecosystem,left by domestic livestock.Additionally,fences intended to delineate conservation areas or to separate each type of grassland use,have brought about profound side effects on wildlife through fragmentation of their habitats.It is recommended to set up the fence in a more ecological way,which can be achieved by bypassing the wildlife frequent pathway and using mate-rials devoid of steel barb.Only considering both opportunities and problems simultaneously,can the wildlife protection on the QP be sustained.
文摘原发中枢神经系统淋巴瘤(primary central nervous system lymphoma,PCNSL)是仅累及脑实质、脊髓、眼、颅神经及脑膜,无中枢神经(central nervous system,CNS)以外部位受累的一类结外非霍奇金淋巴瘤。目前,治疗主要是以大剂量甲氨蝶呤为基础的化疗,联合大剂量化疗/自体干细胞移植(autologous stem cell transplantation,auto-HSCT)或减量的全脑放疗。但缓解率较低,不良反应较大。研究显示,10%~15%的患者对诱导化疗原发耐药,即使治疗有效,仍有50%的患者复发。近年来,PCNSL遗传学和分子的研究极大地促进了生物学机制的理解。小分子药物和靶向药物治疗的前景可观,有望减少不良反应的同时,提高患者缓解率、延长寿命。本文主要对目前PCNSL的新治疗方向进行综述。
