Markers for Global Climate Change and Its Impact on Social, Biological and Ecological Systems: A Review

Present article sketches out major climate induced changes in marine, aquatic and terrestrial life. Few important biomarkers such as ecological, meteorological, socioeconomic, thermal, biophysical and biological, behavioral markers of climate change and global environmental stress have been highlighted to predict the future challenges and finding appropriate solutions. Though, so many climate change induced effects are visible but few unpredictable effects may be seen in future. Therefore, all such effects have been acknowledged, and tried to find appropriate solutions. Most visible effect is collection of high amounts of carbon dioxide in the atmosphere which is responsible for green house effect and causing natural calamities round the globe. It is not only jeopardized the survival of terrestrial, fresh water animals mainly planktons, bottom dwellers;coral reefs, algae, fish fauna in marine environment belong to different taxon but also responsible for disruption of ocean’s food web due to non-assimilation of extra carbon dioxide by the ocean water. There is a sharp decline in fresh water and sea shore micro-flora and micro-fauna. Other major visible effects are loss of biodiversity, depletion of forests, land degradation, severe floods and draughts. On other hand sudden changes in weather conditions causing irreparable devastations due to hurricanes and typhoons, storms, lightening, earthquakes and tsunamis are normally on rise. Both economic and ecological breakdowns are occurring more frequently which are more impactful and persistent. Climate change is major human health stressor;it is making fragmentation of socio-cultural bonds and reducing fertility of soil finally crop production. Climate change is imposing non-adaptive forced human migration, territorial conflicts, decreasing ecosystem productivity, disease out breaks, and impelling unequal resource utilization.

GC-MS Analysis and in Vitro Antimicrobial Susceptibility of <i>Foeniculum vulgare</i>Seed Essential Oil

Essential oil from seeds of Foeniculum vulgare was extracted on Clevenger apparatus. Essential oil was analyzed on Gas-Chromatography-Mass spectrometry (GC-MS) from which thirty six components were identified, among which 6 major and 30 minor components having different structural formulae and molecular weight representing total 99.98% of oil. Essential was investigated for its antibacterial and antifungal activity against seven infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. The Foeniculum vulgare essential oil showed the Diameter of Inhibition Zone (DIZ) ranging from 19.4 ± 0.07 - 26.4 ± 0.09 mm at a concentration level of 28 μg/disc in all the ten strains tested. The minimum inhibitory concentration (MIC) of essential oil against bacterial and fungal strains was obtained in the range of 7.0 - 56 μg/ml. Antibacterial and antifungal activity of Foeniculum vulgare essential oil is due to the presence of certain secondary plant metabolites such as terpenoids, steroids and flavonoids, esters and acids which are identified in the essential oil. The oil components can be further studied for their biological activity and overcome the problem of drug resistance in microbes.

High Cholesterol Disorders, Myocardial Infarction and Its Therapeutics

The current review article discusses several conditions linked to high cholesterol, including their causes, diagnoses, and treatments. Cardiovascular disease is one of the leading causes of death worldwide, and hypercholesterolemia is a standalone risk factor for the condition. Numerous cardiovascular illnesses (CVDs) and disorders, including myocardial infarction, hypertension, peripheral vascular diseases, and coronary heart diseases, were highlighted in this article. Low density lipoprotein (LDL) is the key factor in the majority of CVDs. It adheres to fatty deposits and forms plaques inside artery lumen, preventing the heart and other body organs from receiving enough blood and oxygen. Cardiomyopathies and dyslipidemias are the most common causes of morbidity and mortality in men and place a considerable health cost on society. Age, hypertension, obesity-induced blood pressure, and a rise in plasma cholesterol are all factors that raise the risk of coronary heart disease (CHD). Calorie intake must be controlled, meal composition must be changed, a natural cycle must be established, and circadian timeliness must be maintained in order to live a healthy life. To lower the risks of metabolic and cardiovascular diseases, try to go vegetarian and always consume traditional plant-based diets and herbal remedies. Exercise yoga and aerobics every day to stay physically healthy, and have your blood pressure, blood sugar, and cholesterol levels checked on a regular basis. Follow your family doctor’s recommendations for managing high cholesterol risks and diagnosing CVD, obtain a lipid profile, take the recommended medications, maintain composure, and adopt a positive outlook for a long and healthy life.

Dietary and Nutritional Value of Fish Oil,and Fermented Products

Present review article explains the dietary and nutritional value of various fish derived natural food products.Fish is a good source of important nutrients such as proteins,fats,vitamins and minerals.Fish oil contains polyunsaturated fatty acids(PUFAs)mainly omega-3 fatty acids,eicosapentaenoic acid(EPA),docosahexaenoic acid(DHA),and eicosanoids.Fish contains high-quality protein(~14-16 percent)and is consumed worldwide.This article also emphasizes therapeutic uses of fish nutrients and oil in healing of wounds,hyper pigmentation,dermatitis,and in cardiovascular risks.Fish oil polyunsaturated fatty acids(PUFAs)are highly beneficial in cardiovascular problems and dermatitis.Fish oil is good for skin-related diseases such as photo-ageing and melanogenesis These also affect anticancer,wound healing and anti-depressant activity.In the present review various local,national,and international processed fish derived food currently available in the market fish dishes have been mentioned.

Antimicrobial Activity of Fruit Latexes from Ten Laticiferous Plants

In the present investigation antibacterial activity of latexes from ten Indian plant species Spondias dulcis (Amra), Diospyros melanoxylon (Tendu), Terminalia bellirica (Wahera), Ficus glomerata (Gular), Phyllanthus emblica (Awla), Thevetia nerifolia (Kaner), Carica papaya (Papita), Calotropis procera (Ak), Ficus benghalensis (Bargad), Atrocarpus heterophyllus (Kathal) collected from Go-rakhpur, North India were determined in various in vitro systems. MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values were determined in growth inhibitory bioassays by using different increasing concentrations of various latex extracts. Latex samples were diluted by using serial micro dilution method up to 10-10 method with Luria broth culture medium. These values were obtained significantly 2 - 3 times lower than that of broad spectrum antibiotic drugs. Besides this, inhibition zone diameters were measured in agar disc diffusion assay. A known volume i.e. 0.1 - 20 μg/μl of each latex were coated on separate sterile filter paper discs (Whatman No. 1) measuring 6 mm in size. Latex fractions registered significantly higher growth inhibition than that of broad spectrum antimicrobial drugs. Present study indicates the potential use of shows that both latex and its components and latex as are valuable source of medicinal products/active principles that can be used for treatment of life threatening infectious diseases. Because of higher inhibitory and cidal potential obtained in latexes than the synthetic drugs these that could lead to become efficient phytomedicines mainly to have and develop as complete drug formulations against to control infectious microbes.

Effects of plant latex based anti-termite formulations on Indian white termite <i>Odontotermes obesus</i>(Isoptera: Odontotermitidae) in sub-tropical high infestation areas

In the present investigation various bioassays were conducted to evaluate the anti-termite efficacy of plant latex based formulations to control population of Indian white termite in subtropical soil. Results reveal that crude latex, its fractions and combinatorial fractions have shown very high toxicity against O. obesus. The LD50 values for different latex fractions of 24 h were in a range of 5.0-17.613 μg/mg while combined mixtures of Calotropis procera have shown synergistic activity against termites and caused comparably high mortality with LD50 1.987-6.016 μg/mg. The mortality rate was found dose and time dependent as it was found to be increased with an increase in dose and exposure period. In olfactometry tests, C. procera latex solvent fractions have shown significant repellency at a very low dose 0.010-0.320 μg/mg. Interestingly, solvent fractions have significantly repelled large numbers of worker termites due to volatile action of active components of latex and different additives. ED50 values obtained in crude latex were 0.121 μg/mg body weights while combinatorial formulations have shown ED50 in between 0.015-0.036 μg/mg. Statistical analysis of repelled and un-repelled termites gave a low Chi-square value (X2 value = 0.890) which is an indicator of independence of repellent action in randomly selected termite groups. In field experiments pre-soaked cotton threads impregnated with Calotropis procera crude latex were tagged around tree trunks of Tectona grandis provided a wider protection against O. obesus. By employing these pre-coated threads, termite infestation and tunneling activity were significantly decreased (p Calotropis procera possesses enough anti-termite potential against Indian white termite, O. obesus population. If used these, formulations may also provide wide a range of control against other kinds of pests including house hold, medical and veterinary. However, Calotropis procera latex based formulations can be recommended for effective control of termites in high infestation areas by applying spray, or in form of poison baits or as fumigant in pure form.

Effect of Purified Paper Wasp Ropalidia marginata Venom Toxins on Different Biomolecules in Mice Serum

This study evaluated the effects of purified paper wasp Ropalidia marginata venoms on various biomolecules in the blood serum of albino mice. Changes in the concentration of some important macromolecules, i.e., proteins, free amino acids, uric acid, cholesterol, pyruvic acid, total lipids and glucose were noted down. These alterations were measured after intraperitoneal injection of 40% and 80% 24-hour LD50 purified Ropalidia marginata venom toxin. Serum total protein levels were found to decrease to 78% after 6 hrs, while serum free amino acid levels were significantly increased to 117% 6 hrs after venom injection compared to control. It was also found that serum uric acid levels increased to 138% after 8 hrs of venom injection compared to control. The increase in serum cholesterol i.e. (101% and 106%) and pyruvic acid increased significantly to a maximum value of 106% after 6 hrs of treatment at 40% LD50. Glycogen levels in the gastrocnemius muscle were found to decrease significantly (p-0.05) to 43% and 92% at LD50 after injection of purified Ropalidia marginata venom after 8 h and 80% at LD50 compared to control. Moreover, up to 71% and 81% were obtained at 10 hrs of treatment with the same dose. In the present study, the purified toxins significantly changed the levels of biomolecules in blood serum, indicating their wider effects on cellular physiology due to toxic effects and stress on the animal. These toxins can be good antigens and stimulate immune responses in experimental mice.

Effect of Purified Paper Wasp Ropalidia marginata Venom Toxin Enzyme Activity in Blood Serum, Liver, and Gastrocnemius Muscle of Albino Mice

In the present, investigation effects of sub-lethal dose of purified paper wasp Ropalidia marginata venom toxins were evaluated on important metabolic enzymes i.e. ALP ACP, GPT, GOT, LDH, and AchE enzyme activity in serum, liver, and gastrocnemius muscles of albino mice. Alkaline phosphatase was found to be increased up to 119.9% at the 6th hr of the toxin injection in comparison to control. This elevation may be due to cytolysis. Maximum increase i.e., 153.33% level of glutamate pyruvate transaminase (GPT) was found at 6 hrs of 40% of 24-h LD50 treatment while it was found to be 151.1% at 6 hrs of 24 hr 80% of LD50, venom injection. A significant elevation was observed in LDH activity in serum, liver, and muscles, while the activity of AchE was decreased in serum, liver, and gastrocnemius muscles of albino mice after injecting the sub-lethal dose of Ropalidia marginata venom. This increase in the activity of LDH produces liver damage, massive disintegration and necrosis of hepatic cells. This elevation in LDH level led to a significant increase in the glucose catabolism and elevated oxidative stress in muscle and liver cells. It also displays insufficient oxygen supply and consequently leads to cell death. In experimental animals, venom toxin treatment decreased AchE level, and animals showed muscular paralysis. When mice were treated with 40% and 80% of 24-h LD50 of purified venom caused a significant (p < 0.05) elevation in the level of ACP, GOT, GPT, and LDH while the reduction in ALP and AChE level. Present study will be useful in the development of prototypes for study of pharmacological and therapeutic effects of various venom toxins. For this purpose structure activity relationship of enzyme and venom toxin, its due interaction to various metabolic enzymes and receptors must be explored.

Effects of Purified Indian Cattle Tick Rhipicephalus microplus Saliva Toxins on Various Enzymes in Blood Serum, Liver and Muscles of Albino Mice

In the present investigation, in vivo effects of purified ticks’ saliva toxin were evaluated on the level of certain important cellular metabolic enzymes i.e. acid phosphatase (ACP), alkaline phosphatase (ALP), glutamate pyruvate transaminase, glutamate oxaloacetate transaminase and lactic dehydrogenase. For this purpose, sub-lethal doses, 40% and 80% of 24 h LD50 purified saliva toxins of Rhipicephalus microplus (Canestrini, 1888) were injected subcutaneously in the albino mice. In treated mice saliva toxins targeted membrane-bound enzymes i.e. serum acid phosphatase and alkaline phosphatase, its level was increased from 118.30% to 163.63% at the 6th hr in comparison to the control. Besides this, the levels of serum glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate transaminase (GOT) and lactic dehydrogenase (LDH) also increased up to 161.11% (at 6th hr), 148.27 (at 8th hr) and 125.45% (at 6th hr) respectively in comparison to control. An increase in the level of LDH showed insufficient oxygen supply, massive disintegration of cells and leakage of the enzyme into the circulation. It clearly indicated the toxic effects of saliva toxins on the membrane of blood cells, hepatocytes and myocardial muscle cell functions in albino mice. On the other hand activity of acetyl cholinesterase was reduced by 65.51% at the 6th hr of the saliva toxin injection in comparison to the control. This inhibition of acetyl cholinesterase activity caused the accumulation of acetylcholine molecules at the synaptic junctions and led to prolonged activation of acetylcholine receptors. It caused permanent stimulation of nerves and muscle cells that may result in muscular paralysis and finally death of the animal.

Toxic Effects of Ficus benghalensis Latex Based Combinatorial Formulations on Various Enzymatic Parameters in Indian White Termite Odontotermes obesus

In the present investigation, various bioassays were conducted to evaluate the alteration in levels of various enzymes i.e. alkaline phosphatase, acid phosphatase, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and acetylcholinesterase. For this purpose worker termites were treated with sub-lethal doses of 40% and 80% of 24 hrs LD50 of latex-based combinatorial formulations and observations were taken at 4 hours intervals up to 24 hours. Ficus benghalensis crude latex and its combinatorial mixtures, like C-MLT-A, CU-MLT-A, AQ-MLT, P-MLT and EA-MLT significantly altered the level of enzymes in Odontotermes obesus and this effect found time and dose-dependent. Reduction or increase in enzymes was calculated by using the corresponding control. The maximum decrease in acid phosphatase level was observed at 16 h when termites were treated with 80% of LD50 of B-MLT-B and P-MLT i.e. 82.84% at 16 h of treatment. A similar dose caused a very slight decrease in glutamate pyruvate transaminase at 4 h of treatment but with the increase in time. Further decrease was noted in other successive treatments that were significant at p > 0.05. Similarly, 40% and 80% of LD50 of the C-MLT-B mixture caused significant (p > 0.05) decrease in alkaline phosphatase and acid phosphatase level at 16 h treatment i.e. 93.42%, 89.46% and 95.89%, 88.17% respectively. The level of acetylcholinesterase was also found to be decreased when termites were treated with 40% and 80% of LD50 of C-MLT-B mixture i.e. 92.72% and 97.27% respectively. All the above alterations noted in levels of various enzymes confirm the action of latex ingredients on worker termites that were anti-feedant or deterrent types. These ingredients can be used to control not only termites but also other phytophagous insects.

Termiticidal and Biochemical Activity of Combinatorial Essential Oil Ingredients of Tagetes erecta on Indian White Termite Odontotermes obesus

In present investigation, various bio-molecular parameters i.e. glycogen, protein, amino acid, DNA, RNA and lipid, were determined for evaluation of anti-termite efficacy of Tagetes erecta essential oil based combinatorial formulations against Indian white termite Odontotermes obesus. For this purpose, 40% and 80% of 24 hrs of LD50 values of various formulations were provided for treatment of termite workers. Observations were taken at 4 hrs time interval up to 16 hrs to know inhibitory activity of these combinatorial mixtures on termite species Odontotermes obesus. Significant (p > 0.05) alterations were observed in the level above mentioned bio-molecules when termites were treated with different combinatorial essential oils ingredients of Citrus maxima. Combinatorial mixtures of Tagetes erecta essential oils have shown synergistic activity against termites. This study will provide an overall sustainable way to termite control in crop field, gardens and houses. It also suggests use of essential oils as better alternative of synthetic termiticides as these are safer for environmental and human health.

Antimicrobial Activity of Purified Toxins from Crossopriza lyoni (Spider) against Certain Bacteria and Fungi

Toxins from spider venom Crossopriza lyoni were subjected to purify on a Sepharose CL-6B 200 column. These were investigated for its antibacterial and antifungal activity against 13 infectious microbial pathogenic strains. Antimicrobial susceptibility was determined by using paper disc diffusion and serial micro-dilution assays. Triton X-100 (0.1%) proved to be a good solubilizing agent for toxin/proteins. Higher protein solubilization was observed in the supernatant than in the residue, except TCA. The elution pattern of purified and homogenized sting glands displayed two major peaks at 280 nm. First one was eluted in fraction no. 43 - 51 while second one after fraction no. 61 - 90. From gel filtration chromatography total yield of protein obtained was 67.3%. From comparison of gel chromatographs eluted toxins peptide molecular weight was ranging from 6.2 - 64 kD. Toxin peptides have shown lower MIC values i.e. 7.5 - 15 μg/ml against K. pneumoniae, E. coli, L. acidophilus, B. cereus;against S. aureus and M. luteus that the broad spectrum antibiotics i.e. tetracycline and ampicillin. In tests, larger inhibition zone diameter was obtained in comparison to control. Diameter of inhibition zones obtained in spider toxins at a concentration range of 197.12 - 0.96 g/ml for E. coli was 17.86 ± 0.21, Bacillus cereus 19.13 ± 0.21, L. acidophilus 16.83 ± 0.25, Micrococcus luteus 18.46 ± 0.17, S. aeurus 16.23 ± 0.19, Klebsiella pneumoniae 21.83 ± 0.16, Salmonella typhi 16.16 ± 0.21, Vibrio cholera 18.66 ± 0.21, Pseudomonas aeruginosa 18.66 ± 0.21, Aspergillus niger 22.9 ± 0.24, Candida albicans 24.66 ± 0.28, Rhizopus stolonifer 21.1 ± 0.16. Spider toxins generate cytotoxic effect on bacterial cells that results in heavy cell death. No doubt spider toxins can be used as alternate of broad spectrum antibiotics.

Toxic Effects of Citrus maxima Based Combinatorial Formulations on Important Metabolic Enzymes in Indian White Termite Odontotermes obesus

In the present study, Citrus maxima essential crude oil extract was used to prepare combinatorial formulations and workers of Indian white termite Odontotermes obesus were treated topically with 40% and 80% of 24 hr LD50 values of these formulations. In subsequent bioassays levels of various enzymes i.e. alkaline phosphatase, acid phosphatase, glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and acetylcholinesterase were evaluated to determine the anti-termite efficacy of Citrus maxima essential oil based combinatorial formulations. S-RET-A, S-RET-B and S-RET-C caused significant (p > 0.05) decrease in glutamate oxaloacetate transaminase i.e. 87.47%, 86.81% and 81.77% & 82.04%, 79.39% and 74.75% respectively at 16 h treatment. In vivo exposure of 40% and 80% of LD50 of combinatorial formulations caused very significant (p > 0.05) reduction in all the test enzymes i.e. alkaline phosphatase, acid phosphatase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase expect acetylcholinesterase levels after 16 h all tested treatments in comparison to control. Both dose-response and time period were found important in physiological alteration in levels of various enzymes. Combinatorial mixtures of Citrus essential oils have shown synergistic activity against termites. The research findings of the present study would help termite control in crop fields, gardens and houses in a sustainable way.

Toxicity of Tagetes erecta Essential Oil Based Combinatorial Formulations on Various Metabolic Enzymes in Indian White Termite Odontotermes obesus

Plant essential oils and their constituents have proven to be very effective against insects, especially termites. They are the best alternative to synthetic pesticides that are harmless to the environment and human health. In the present study, different enzymes, namely alkaline phosphatase, acid phosphatase, glutamate-oxaloacetate transaminase, glutamate-pyruvate transaminase and acetylcholinesterase, were evaluated in Indian termite Odontotermes obesus in a combination preparation based on Tagetes erecta essential oil. For the study of anti-termite effects against worker termites were treated topically with 40% and 80% of the 24-hour LD50 values of various combination formulations. Subsequent bioassays at 40% and 80% of LD50 of combinatorial preparations S-AST-A, S-AST-B, and S-AST-C resulted in a significant (p > 0.05) decrease in glutamate-oxaloacetate transaminase. After 16 hours of treatment, they were 87.92%, 80.17%, 89.30%, 79.17%, 81.92% and 73.17% respectively. In vivo exposures of 40% and 80% of the LD50 of the combination formulation caused a highly significant (p > 0.05) reduction (p > 0.05) of all test enzymes tested compared to controls. The effects of different oil formulations exhibited time- and dose-dependent responses, resulting in physiological changes in the concentrations of various enzymes. The combined mixture of Tagetes erecta essential oils has significantly better anti-termite ability compared to inorganic insecticides. Findings from this study will help support termite control in fields, gardens and homes in a sustainable way, without the downsides of insecticide resistance and pollution. These could potentially be used to produce commercial formulations for use against pests.

Biochemical Alterations and Termiticidal Activity of Combinatorial Essential Oil Ingredients of Citrus maxima on Indian White Termite Odontotermes obesus

Essential oils are better alternative for synthetic termiticides with the inherent resistance by insects, environmental and health effects on humans. In present investigation, various bio-molecules i.e. glycogen, protein, amino acid, DNA, RNA and lipid were evaluated to determine the anti-termite efficacy of Citrus maxima essential oil based combinatorial formulations against Indian white termite Odontotermes obesus. For this purpose, termites were treated topically with 40% and 80% of 24 hr LD50 values of various combinatorial formulations. A significant (p > 0.05) alteration was noted in all the above biomolecules at various time intervals. Both dose response and time period was found important in physiological alteration in levels of various bio-molecules. Combinatorial mixtures of Citrus essential oils have shown synergistic activity against termites. The research outcomes of present investigation would help to open sustainable way to termite control in crop field, gardens and houses.

Effect of Ficus benghalensis Latex Based Combinatorial Formulations on Various Bio-Molecules in Indian White Termite Odontotermes obesus

In present investigation, various bioassays were conducted to evaluate the alteration in levels of certain biomolecules, such as glycogen, protein, amino acid, DNA, RNA and lipids. For this purpose, worker termites were treated with sub-lethal dose 40% and 80% of 24 hrs LD50 values and observations were taken at 4 hrs interval up to 24 hrs. Crude latex and its combinatorial mixtures, like S-MLT-A, B-MLT-A, C-MLT-A, CU-MLT-A, AQ-MLT significantly altered level of bio-molecules in Odontotermes obesus. This effect was found time and dose dependent. Reduction or increase in biomolecules was calculated by using corresponding control. Maximum decrease in glycogen level was observed at 16 h when termites were treated with 80% of LD50 of Ficus benghalensis aqueous extract i.e. 56.88% at 16 h of treatment. A similar dose caused very slight decrease in lipid contents at 4 h of treatment but it was found further significantly (p > 0.05) decreased in other successive treatments. 40% and 80% of LD50 of C-MLT-B mixture caused significant (p > 0.05) decrease in DNA and RNA level at 16 h treatment. The level of DNA and RNA level was recorded 83.90%, 90.18% and 85.42% and 74.05% respectively. Similarly, total proteins were also found to be decreased with 40% and 80% of LD50 of C-MLT-B mixture i.e. 71.47% and 66.45% respectively. All these alterations found in levels of various bio-molecules confirm the action of latex ingredients on worker termites that was antifeedant or deterrent types. These ingredients can be used to control not only termites but also other phytophagous insects in a sustainable and eco-friendly way.

Antimicrobial Activity of Purified Toxins from Yellow Wasp Polistes flavus (Vespidae) against Certain Bacteria and Fungi

Yellow Wasp Polistes flavus venom toxins were isolated and purified on a Sepharose CL-6B 200 column. Purified proteins were investigated for its antibacterial and antifungal activity against 13 infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. Triton X-100 (0.1%) proved to be a good solubilizing agent for toxin/proteins. Higher protein solubilization was observed in the supernatant than in the residue, except TCA (tri-chloroacetic acid). The elution pattern of purified and homogenized sting glands exhibited two major peaks at 280 nm in fraction No. 41 - 61 and 81 - 101. The total yield of protein was 69.21% and specific activity was determined in each fraction. Molecular weights in protein fractions were ranging from 6 - 70 kD. MIC (Minimum Inhibitory Concentration) values were 12.3 μg/ml against K. pneumonia 12.3 μg/ml against E. coli and L. acidophilus, 24.6 μg/ml against B. cereus;49.24 μg/ml against S. aureus and M. luteus. By agar disc diffusion method, the diameter of inhibition zones in mm in presence of yellow wasp toxins is at a concentration range of 98.56 - 6.9 μg/ml E. coli 18.36 ± 0.14, Bacillus cereus 14.566 ± 0.21, L. acidophilus 18.10 ± 0.21, Micrococcus luteus 18.76 ± 0.19, S. aeurus 17.36 ± 0.43, Klebsiella pneuminiae 19.56 ± 0.21, Salmonella typhi 19.96 ± 0.31, Vibrio cholera 21.83 ± 0.22, Pseudomonas aeruginosa 22.90 ± 0.09, Aspergillus niger 21.66 ± 0.16, Candida albicans 23.33 ± 0.26, Rhizopus stolonifer 24.96 ± 0.16 respectively. The antibacterial and antifungal activity of venom toxin may be due to action on cell membrane, its destruction and cell lysis. The wasp toxins may be used as strong biological agents to control microbes.

Climate Induced Virus Generated Communicable Diseases:Management Issues and Failures

In the present review article human diseases caused by various groups of pathogens have been explained with its etiology,epidemiology and treatment.In addition,effect of climatic changes on parasites and pathogens has been demarcated with rising incidences of diseases.In response to environmental changes,mainly external and internal microenvironment of body and drug regimens taken by patients;virus is regularly changing its form and new mutant variants are coming out.These are circulating in many Indian states and cross border countries and causing high infectivity and mortality in human patients.These variants with new mutations are challenging existing drugs and other prophylactic measures and massively disrupting functions of a tissue,organ,or entire organism.Diseases caused by viruses are showing new trends in virulence,with high infectivity,morbidity and mortality.Due to climatic effect and drug resistance and new mutations in pathogens disease burden has been exacerbated enormously at global level.In all cases of helminthes,protozoan’s,fungi,bacteria,virus pathogens and parasites available drug structure seem to be failed or their usefulness has been much reduced due to evolution of new mutant variants with multiple drug resistance.There are serious failures at the level of operation,management and control of disease.The utmost failure is due to lack of appropriate vaccine,drug regimens,clinical care and awareness among people.These are major reasons that is why diseases become uncontrolled and unmanageable.

Global Effect of Climate Change on Seasonal Cycles,Vector Population and Rising Challenges of Communicable Diseases:A Review

This article explains ongoing changes in global climate and their effect on the resurgence of vector and pathogen populations in various parts of the world.Today,major prevailing changes are the elevation of global temperature and accidental torrent rains,floods,droughts,and loss of productivity and food commodities.Due to the increase in water surface area and the longer presence of flood water,the breeding of insect vectors becomes very high;it is responsible for the emergence and re-emergence of so many communicable diseases.Due to the development of resistance to chemicals in insect pests,and pathogens and lack of control measures,communicable zoonotic diseases are remerging with high infectivity and mortality.This condition is becoming more alarming as the climate is favoring pathogen-host interactions and vector populations.Rapid changes seen in meteorology are promoting an unmanageable array of vector-borne infectious diseases,such as malaria,Japanese encephalitis,filarial,dengue,and leishmaniasis.Similarly,due to unhygienic conditions,poor sanitation,and infected ground and surface water outbreak of enteric infections such as cholera,vibriosis,and rotavirus is seen on the rise.In addition,parasitic infection ascariasis,fasciolosis,schistosomiasis,and dysentery cases are increasing.Today climate change is a major issue and challenge that needs timely quick solutions.Climate change is imposing non-adaptive forced human migration territorial conflicts,decreasing ecosystem productivity,disease outbreaks,and impelling unequal resource utilization.Rapid climate changes,parasites,pathogens,and vector populations are on the rise,which is making great threats to global health and the environment.This article highlighted the necessity to develop new strategies and control measures to cut down rising vector and pathogen populations in endemic areas.For finding quick solutions educational awareness,technology up-gradation,new vaccines,and safety measures have to be adopted to break the cycle of dreadful communicable diseases shortly.

Tick-borne Diseases,Transmission,Host Immune Responses,Diagnosis and Control

Present review article explains tick-borne diseases,transmission,host immune responses,diagnosis and control in relation to climatic variations.Ticks are hematophagous ectoparasites which suck large volumes of blood from livestock and humans.They release large numbers of protozoans,bacteria,rickettsia and viral pathogens during blood feeding and transmit disease pathogens through saliva.Due to heavy blood sucking by ticks animals face significant blood and weight loss that affect their overall health.Due to more severe illness,high economic losses were noted in livestock.This article highlights medically important tick borne diseases in man and livestock,its pathogenesis,diagnosis and treatment methods.The present article emphasizes invasion of hosts,host-pathogen interactions,tick saliva toxin induced host immune responses and biological effects.This article highlighted various tick control methods i.e.physical killing,acaricidal,biological,hormonal,genetic and immunological methods such as administration of protective antibody and vaccines for disease control in human being and his livestock.The authors suggest non-chemical environmentally safe methods for successful control of tick borne diseases to kill cattle,bird and canine invading ticks.