In order to investigate the association of G＋1688A （Ser563Asn） polymorphism of platelet endothelial cell adhesion molecule-1 （PECAM-1） gene with myocardial infarction （MI） in the Chinese Han population, the G＋...In order to investigate the association of G＋1688A （Ser563Asn） polymorphism of platelet endothelial cell adhesion molecule-1 （PECAM-1） gene with myocardial infarction （MI） in the Chinese Han population, the G＋1688A polymorphism in PECAM-1 gene was detected by polymerase chain reaction-restriction fragment-length polymorphism （PCR-RFLP） method among 502 subjects, including 218 patients with MI and 284 controls. The results showed that there was significant difference in AA frequencies of genotype G＋1688A polymorphism between case and control groups （39% vs 24%, P〈0.001）. A similar trend was observed on the allele frequencies （A/G： 62% vs 49%, P〈0.001）. Among the subjects with high serum total cholesterol level or high systolic blood pressure level, the variant AA genotype was associated with high risk of MI （adjusted OR, 2.13; 95% CI, 1.08 -4.41 and adjusted OR, 2.53; 95%CI, 1.63-3.63）. The single nucleotide polymorphism （SNP） at position ＋1688 in the exon 8 of PECAM-1 gene was associated with MI and the allele A might be a risk factor for MI in the Chinese Han population.展开更多
Natriuretic peptide precursor A(NPPA)is synthesized,stored,and released by atrial myocytes.Previous studies have shown that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis...Natriuretic peptide precursor A(NPPA)is synthesized,stored,and released by atrial myocytes.Previous studies have shown that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis.Rs5065 NPPA gene polymorphism leads to the translation of NPPA with two additional arginines and has been suggested to be associated with salt-sensitive hypertension.The purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphism and the risk of coronary heart disease(CHD)in Chinese Han population.We genotyped the single nucleotide polymorphism(SNP)rs5065 NPPA in the human NPPA gene in 1861 sex-and age-matched subjects,comprising of 904 CHD cases and 957 controls of Chinese Han population.Genotyping of SNP was performed with Taqman SNP allelic discrimination assays by means of an ABI 7900HT.Our study showed that the frequencies of rs5065 NPPA C allele in the case and the control groups were 0.012 and 0.005,respectively.There was significant difference in C allele frequency distribution between the two groups(OR=2.607,95%CI:1.197–5.678,P=0.012).In the case group,there was significant difference between smokers and nonsmokers with subjects carrying C allele(P=0.037),and no significant difference in gender,age,fasting total cholesterol(TC),triglycerides(TG),fasting plasma glucose(FPG),body mass index(BMI),and blood pressure(BP)between the cases and the controls(P>0.05).Our results suggest that the C allele of rs5065 NPPA gene polymorphism may be associated with the risk of CHD.展开更多
文摘In order to investigate the association of G＋1688A （Ser563Asn） polymorphism of platelet endothelial cell adhesion molecule-1 （PECAM-1） gene with myocardial infarction （MI） in the Chinese Han population, the G＋1688A polymorphism in PECAM-1 gene was detected by polymerase chain reaction-restriction fragment-length polymorphism （PCR-RFLP） method among 502 subjects, including 218 patients with MI and 284 controls. The results showed that there was significant difference in AA frequencies of genotype G＋1688A polymorphism between case and control groups （39% vs 24%, P〈0.001）. A similar trend was observed on the allele frequencies （A/G： 62% vs 49%, P〈0.001）. Among the subjects with high serum total cholesterol level or high systolic blood pressure level, the variant AA genotype was associated with high risk of MI （adjusted OR, 2.13; 95% CI, 1.08 -4.41 and adjusted OR, 2.53; 95%CI, 1.63-3.63）. The single nucleotide polymorphism （SNP） at position ＋1688 in the exon 8 of PECAM-1 gene was associated with MI and the allele A might be a risk factor for MI in the Chinese Han population.
文摘Natriuretic peptide precursor A(NPPA)is synthesized,stored,and released by atrial myocytes.Previous studies have shown that NPPA plays a significant role in the regulation of coronary circulation and in atherosclerosis.Rs5065 NPPA gene polymorphism leads to the translation of NPPA with two additional arginines and has been suggested to be associated with salt-sensitive hypertension.The purpose of the present study was to investigate the relationship between the rs5065 NPPA gene polymorphism and the risk of coronary heart disease(CHD)in Chinese Han population.We genotyped the single nucleotide polymorphism(SNP)rs5065 NPPA in the human NPPA gene in 1861 sex-and age-matched subjects,comprising of 904 CHD cases and 957 controls of Chinese Han population.Genotyping of SNP was performed with Taqman SNP allelic discrimination assays by means of an ABI 7900HT.Our study showed that the frequencies of rs5065 NPPA C allele in the case and the control groups were 0.012 and 0.005,respectively.There was significant difference in C allele frequency distribution between the two groups(OR=2.607,95%CI:1.197–5.678,P=0.012).In the case group,there was significant difference between smokers and nonsmokers with subjects carrying C allele(P=0.037),and no significant difference in gender,age,fasting total cholesterol(TC),triglycerides(TG),fasting plasma glucose(FPG),body mass index(BMI),and blood pressure(BP)between the cases and the controls(P>0.05).Our results suggest that the C allele of rs5065 NPPA gene polymorphism may be associated with the risk of CHD.
