Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157:H7 through transcriptomic analysis

Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.

Analysis of tight oil accumulation conditions and prediction of sweet spots in Ordos Basin: A case study

Tight sandstone reservoirs are widely developed in the Mesozoic Yanchang Formation of the Ordos Basin,China.There is a lack of understanding on the sedimentary setting,source-reservoir relationship and oil accumulation conditions in this area.In this study,through the comprehensive analysis of the distri-bution of tight oil,we evaluated the properties and petrological features of reservoir,geochemical characteristics of source rocks,the source-reservoir relationship,as well as the trapping,preservation and accumulation conditions of tight oil in the Chang 7 Member,and predicted the sweet spots of tight oil in the study area.The results show that the Chang 7 Member is a typical low-porosity and ultra-low permeability reservoir with great tightness,small pore throat and high capillary pressure,and must have been of near-source accumulation.The source rocks are mainly developed in the Chang 7_(3) submember,and the reservoirs mainly occur in the Chang 7_(1) and Chang 7_(2) submembers,forming a combination mode of“lower source rock and upper reservoir”.Sandbodies with good connectivity and fractures being well developed in local areas are the main hydrocarbon transport systems.The abnormal high pressure caused by hydrocarbon generation and pressurization is the main driving force of tight oil accumulation.The mode of hydrocarbon transportation is dominated by the vertical or lateral migration from under-lying source rocks or adjacent source rocks to reservoirs within a short distance.Following the integrated evaluation of lithology,physical properties and oil saturation of reservoirs and geochemical character-istics of source rocks,we grouped the sweet spots of Chang 7 Member into three types:Type I,Type II and Type III.Among others,the Type I sweet spots are the best in terms of porosity,permeability and source rock thickness and hydrocarbon enrichment which should be the focus of oilfield development.This study lays an important foundation for the economic and efficient development of tight oil in the Chang 7 Member of Heshui area,and has important implications on tight sandstone reservoirs in other regions of Ordos Basin in China.