Facile Preparation of Dopamine-Modified Magnetic Zinc Ferrite Immobilized Lipase for Highly Efficient Synthesis of OPO Functional Lipid

1,3-Dioleoyl-2-palmitoylglycerol(OPO)has been a hotspot of functional oils research in recent years,but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability,large-scale industrial application is difficult.In this study,the prepared magnetic ZnFe_(2)O_(4) was functionalized with dopamine to obtain ZnFe_(2)O_(4)@PDA,and the nano-biocatalyst ZnFe_(2)O_(4)@PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase(RML)via a cross-linking method.The existence of RML on ZnFe_(2)O_(4)@PDA was confirmed by XRD,FTIR,SEM,and TEM.This strategy proved to be simple and effective because the lipase immobilized on magnetic nanoparticles could be quickly recovered using external magnets,enabling reuse of the lipase.The activity,adaptability to a high temperature,pH value,and operational stability of immobilized RML were superior to those of free RML.After optimizing the synthesis conditions,the OPO yield was 42.78%,and the proportion of PA at the sn-2 position(PA-Sn2)was 54.63%.After the first four cycles,the activity of ZnFe_(2)O_(4)@PDA@RML was not significantly affected.The magnetically immobilized lipase has good thermal stability,long-term storage stability,reusability,and high catalytic activity.It can be used as a green and efficient biocatalyst to synthesize the OPO functional lipid.

Experimental study on thermo-hydraulic performances of nanofluids flowing through a corrugated tube filled with copper foam in heat exchange systems

Thermo-hydraulic characteristics of TiO_2-water nanofluids in thin-wall stainless steel test tubes(corrugated tube and circular tube) filled with copper foam(40 PPI) are experimentally investigated and compared with those in test tubes without copper foam. The effects of nanoparticle mass concentration on flow and heat transfer performances are investigated. In addition, the mutual restriction relationships between Reynolds number(Re), Nusselt number(Nu) and resistance coefficient(f) are discussed respectively. Also, the comprehensive coefficient of performance(CCP) between heat transfer and pressure drop is evaluated. The results show that core-enhancement region for heat transfer using experimental tubes filled with copper foam is notably different from that of tubes without copper foam. There is a corresponding Reynolds number(about Re = 2400) for the maximum CCP of each condition. And the heat transfer can be enhanced dramatically and sustained at 8000 ≤ Re ≤ 12000.