Osteoclast is critical in skeletal development and fracture healing,yet the impact and underlying mechanisms of their metabolic state on these processes remain unclear.Here,by using osteoclast-specific small GTPase Rh...Osteoclast is critical in skeletal development and fracture healing,yet the impact and underlying mechanisms of their metabolic state on these processes remain unclear.Here,by using osteoclast-specific small GTPase Rheb1-knockout mice,we reveal that mitochondrial respiration,rather than glycolysis,is essential for cathepsin K(CTSK)production in osteoclasts and is regulated by Rheb1 in a mechanistic target of rapamycin complex 1(mTORC1)-independent manner.展开更多
BACKGROUND Pulmonary lymphoepithelioma-like carcinoma(PLELC)is a rare type of nonsmall-cell lung cancer.Stomach lymphoepithelioma-like carcinoma(LELC)metastasis secondary to PLELC has not been reported recently.CASE S...BACKGROUND Pulmonary lymphoepithelioma-like carcinoma(PLELC)is a rare type of nonsmall-cell lung cancer.Stomach lymphoepithelioma-like carcinoma(LELC)metastasis secondary to PLELC has not been reported recently.CASE SUMMARY A 64-year-old female was admitted to our hospital for a regular gastroscopy examination with a 6-year history of surgical resection for left PLELC.Positron emission tomography/computed tomography suggested high accumulation of 18F-fludeoxyglucose in the gastric cardia region.Upper gastrointestinal endoscopy confirmed a large mass at the stomach fundus.Immunohistochemistry(IHC)of the biopsy suggested metastatic stomach LELC.Proximal gastrectomy showed that this 6.5 cm×5.0 cm mass was located in the stomach fundus near the cardia.Histopathological examination showed a poorly differentiated carcinoma with prominent lymphoplasmacytic infiltration.IHC demonstrated that the tumor was positive for CK(AE1/AE3),p63,p40,p53,Ki-67(70%),and EGFR(3+)and negative for CK7,CK20,Her2,and CD10.In situ hybridization analysis showed positive staining Epstein-Barr virus-encoded RNA.Tumor programmed cell death ligand 1(PD-L1)expression score was 98%,and the combined positive score was 100,with no evidence of microsatellite instability.Thus,the patient was unequivocally diagnosed with metastatic stomach LELC secondary to pulmonary LELC.After discharge,this patient underwent PD-1 inhibitor treatment(toripalimab,240 mg)every 3 wk for ten cycles,and she has had no tumor recurrence.CONCLUSION For gastric LELC metastasis,PD-1 inhibitor therapy could become a new therapeutic approach,though there is still no evidence from large data sets to support this.展开更多
目的评估普通电切镜、等离子电切镜应用在前列腺增生(prostatic hyperplasia,PH)手术治疗中的效果及安全性。方法纳入2021年7月—2023年1月上海中医药大学附属第七人民医院泌尿外科的76例PH患者,参照随机数字表法分为对照组(纳入38例,...目的评估普通电切镜、等离子电切镜应用在前列腺增生(prostatic hyperplasia,PH)手术治疗中的效果及安全性。方法纳入2021年7月—2023年1月上海中医药大学附属第七人民医院泌尿外科的76例PH患者,参照随机数字表法分为对照组(纳入38例,行普通电切镜手术)、观察组(纳入38例,行等离子电切镜手术)。评价两组临床有效率、围手术期参数、尿流动力学、国际前列腺症状评分(international prostate symptom score,IPSS)、射精功能评分(ejaculatory function score,CIPE-5)、生活质量综合评定问卷(comprehensive quality of life assessment questionnaire-74,GQOLI-74)、并发症。结果(1)观察组临床总有效率高于对照组,手术用时、住院时间、尿管留置时间短于对照组,手术出血量及并发症总发生率低于对照组(P<0.05);(2)术前,两组尿流动力学、IPSS、CIPE-5、GQOLI-74比较,差异无统计学意义(P>0.05);术后,观察组尿流动力学、IPSS、CIPE-5、GQOLI-74更佳(P<0.05)。结论在PH手术治疗中采用等离子电切镜手术,有助于增强疗效,优化围手术期参数,减少并发症及性功能障碍,改善排尿功能与生活质量。展开更多
基金supported by Grant Nos.31872799,82070906 and 31701033 from the National Natural Science Foundation of ChinaGrant Nos.2020A1515011189 from Guangdong Basic and Applied Basic Research Foundation of China。
文摘Osteoclast is critical in skeletal development and fracture healing,yet the impact and underlying mechanisms of their metabolic state on these processes remain unclear.Here,by using osteoclast-specific small GTPase Rheb1-knockout mice,we reveal that mitochondrial respiration,rather than glycolysis,is essential for cathepsin K(CTSK)production in osteoclasts and is regulated by Rheb1 in a mechanistic target of rapamycin complex 1(mTORC1)-independent manner.
基金the Zhejiang Provincial Key Project of Research and Development,No.2019C03043National Natural Science Foundation of China,No.82203452+1 种基金Health Science and Technology Plan of Zhejiang Province,No.2022RC165Clinical Research Fund of Zhejiang Medical Association,No.2021ZYC-A68.
文摘BACKGROUND Pulmonary lymphoepithelioma-like carcinoma(PLELC)is a rare type of nonsmall-cell lung cancer.Stomach lymphoepithelioma-like carcinoma(LELC)metastasis secondary to PLELC has not been reported recently.CASE SUMMARY A 64-year-old female was admitted to our hospital for a regular gastroscopy examination with a 6-year history of surgical resection for left PLELC.Positron emission tomography/computed tomography suggested high accumulation of 18F-fludeoxyglucose in the gastric cardia region.Upper gastrointestinal endoscopy confirmed a large mass at the stomach fundus.Immunohistochemistry(IHC)of the biopsy suggested metastatic stomach LELC.Proximal gastrectomy showed that this 6.5 cm×5.0 cm mass was located in the stomach fundus near the cardia.Histopathological examination showed a poorly differentiated carcinoma with prominent lymphoplasmacytic infiltration.IHC demonstrated that the tumor was positive for CK(AE1/AE3),p63,p40,p53,Ki-67(70%),and EGFR(3+)and negative for CK7,CK20,Her2,and CD10.In situ hybridization analysis showed positive staining Epstein-Barr virus-encoded RNA.Tumor programmed cell death ligand 1(PD-L1)expression score was 98%,and the combined positive score was 100,with no evidence of microsatellite instability.Thus,the patient was unequivocally diagnosed with metastatic stomach LELC secondary to pulmonary LELC.After discharge,this patient underwent PD-1 inhibitor treatment(toripalimab,240 mg)every 3 wk for ten cycles,and she has had no tumor recurrence.CONCLUSION For gastric LELC metastasis,PD-1 inhibitor therapy could become a new therapeutic approach,though there is still no evidence from large data sets to support this.
文摘目的评估普通电切镜、等离子电切镜应用在前列腺增生(prostatic hyperplasia,PH)手术治疗中的效果及安全性。方法纳入2021年7月—2023年1月上海中医药大学附属第七人民医院泌尿外科的76例PH患者,参照随机数字表法分为对照组(纳入38例,行普通电切镜手术)、观察组(纳入38例,行等离子电切镜手术)。评价两组临床有效率、围手术期参数、尿流动力学、国际前列腺症状评分(international prostate symptom score,IPSS)、射精功能评分(ejaculatory function score,CIPE-5)、生活质量综合评定问卷(comprehensive quality of life assessment questionnaire-74,GQOLI-74)、并发症。结果(1)观察组临床总有效率高于对照组,手术用时、住院时间、尿管留置时间短于对照组,手术出血量及并发症总发生率低于对照组(P<0.05);(2)术前,两组尿流动力学、IPSS、CIPE-5、GQOLI-74比较,差异无统计学意义(P>0.05);术后,观察组尿流动力学、IPSS、CIPE-5、GQOLI-74更佳(P<0.05)。结论在PH手术治疗中采用等离子电切镜手术,有助于增强疗效,优化围手术期参数,减少并发症及性功能障碍,改善排尿功能与生活质量。
