Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces...Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.展开更多
The brine shrimp Artemia is often abundantly found in hypersaline environments.The larvae(‘nauplii’)hatching from its dormant embryos(‘cysts’)are a crucial live food item for the larval stages of fish and shrimp i...The brine shrimp Artemia is often abundantly found in hypersaline environments.The larvae(‘nauplii’)hatching from its dormant embryos(‘cysts’)are a crucial live food item for the larval stages of fish and shrimp in aquaculture展开更多
1 Introduction Bioflocs is a heterogeneous mixture of the microorganisms,particles,organic polymers and dead cells,etc.(Hargreaves,2006).Among which,heterotrophic bacteria convert efficiently the ammonia nitrogen into...1 Introduction Bioflocs is a heterogeneous mixture of the microorganisms,particles,organic polymers and dead cells,etc.(Hargreaves,2006).Among which,heterotrophic bacteria convert efficiently the ammonia nitrogen into the bacterial protein,which provides the supplementary feed for culture animals and reduces the nitrogen level in the culture system(De Schryver et al,2008).In the closed aquaculture system,the growth of展开更多
1 Introduction The multi-pond solar saltworks inhabit diverse microbial groups in which a gradient of salinities range from seawater to Na Cl precipitation.Along the salinity gradient,the majority of microbial communi...1 Introduction The multi-pond solar saltworks inhabit diverse microbial groups in which a gradient of salinities range from seawater to Na Cl precipitation.Along the salinity gradient,the majority of microbial community changes展开更多
猪圆环病毒病是猪圆环病毒(Porcine circovirus,PCV)引起的一种传染病,特别是感染猪圆环病毒Ⅱ型(Porcine circovirus type 2,PCV2)后,可引起断奶仔猪多系统衰竭综合征、猪皮炎肾病综合征、猪呼吸疾病综合征等,大大增加了养猪成本和防...猪圆环病毒病是猪圆环病毒(Porcine circovirus,PCV)引起的一种传染病,特别是感染猪圆环病毒Ⅱ型(Porcine circovirus type 2,PCV2)后,可引起断奶仔猪多系统衰竭综合征、猪皮炎肾病综合征、猪呼吸疾病综合征等,大大增加了养猪成本和防控难度,给养猪业造成重大经济损失。为积极推进动物疫病净化场创建工作,对1家扩繁场引进的种猪进行PCV2的ELISA和PCR检测。通过采用隔离复合用药治疗,最终实现了PCV2净化。本研究的净化技术措施简便易行,对种猪场PCV2净化工作具有一定的示范借鉴意义。展开更多
基金Supported by the Yangtze Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT_17R81)the Technology Support Project of Tianjin(No.16YFZCNC00810)
文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.
文摘The brine shrimp Artemia is often abundantly found in hypersaline environments.The larvae(‘nauplii’)hatching from its dormant embryos(‘cysts’)are a crucial live food item for the larval stages of fish and shrimp in aquaculture
基金supported by the International Cooperation Research Program of the Ministry of Science & Technology of China (2010DFA32300)Natural Science Foundation of Tianjin City (13JCZDJC28700)China and the Pilot Project for International Cooperation funded by the Province of East-Flanders, Belgium
文摘1 Introduction Bioflocs is a heterogeneous mixture of the microorganisms,particles,organic polymers and dead cells,etc.(Hargreaves,2006).Among which,heterotrophic bacteria convert efficiently the ammonia nitrogen into the bacterial protein,which provides the supplementary feed for culture animals and reduces the nitrogen level in the culture system(De Schryver et al,2008).In the closed aquaculture system,the growth of
基金supported by the International Cooperation Research Program of the Ministry of Science & Technology of China (2010DFA32300)Natural Science Foundation of Tianjin City (13JCZDJC28700)the Pilot Project for International Cooperation funded by the Province of East-Flanders, Belgium
文摘1 Introduction The multi-pond solar saltworks inhabit diverse microbial groups in which a gradient of salinities range from seawater to Na Cl precipitation.Along the salinity gradient,the majority of microbial community changes
文摘猪圆环病毒病是猪圆环病毒(Porcine circovirus,PCV)引起的一种传染病,特别是感染猪圆环病毒Ⅱ型(Porcine circovirus type 2,PCV2)后,可引起断奶仔猪多系统衰竭综合征、猪皮炎肾病综合征、猪呼吸疾病综合征等,大大增加了养猪成本和防控难度,给养猪业造成重大经济损失。为积极推进动物疫病净化场创建工作,对1家扩繁场引进的种猪进行PCV2的ELISA和PCR检测。通过采用隔离复合用药治疗,最终实现了PCV2净化。本研究的净化技术措施简便易行,对种猪场PCV2净化工作具有一定的示范借鉴意义。
