【目的】利用网络药理学的方法探讨杜仲治疗骨质疏松(OP)的可能作用机制。【方法】通过中药系统药理学数据库与分析平台(TCMSP)、文献检索获取杜仲的活性成分,利用反向分子对接服务器(DRAR-CPI)、GeneCards和在线人类孟德尔遗传(OMIM)...【目的】利用网络药理学的方法探讨杜仲治疗骨质疏松(OP)的可能作用机制。【方法】通过中药系统药理学数据库与分析平台(TCMSP)、文献检索获取杜仲的活性成分,利用反向分子对接服务器(DRAR-CPI)、GeneCards和在线人类孟德尔遗传(OMIM)数据库筛选杜仲的活性成分治疗骨质疏松的潜在作用靶点。应用Cytoscape软件构建杜仲的活性成分—作用靶点网络。应用String数据库和Cytoscape软件获得蛋白质相互作用网络。采用Systems Dock Web Site网络服务器与杜仲的活性成分进行分子对接。利用DAVID数据库对杜仲的作用靶点进行基因本体论(GO)分类富集分析和京都基因和基因组百科全书(KEGG)通路富集分析。【结果】从杜仲中筛选出活性成分12个,作用靶标35个。杜仲治疗骨质疏松主要涉及磷脂酰肌醇-3-激酶(PI3K)/丝苏氨酸蛋白激酶Akt、血管内皮生长因子(VEGF)、腺苷酸活化蛋白激酶(AMPK)、Notch、核因子kappaB(NF-κB)、叉头框蛋白(FoxO)、人嗜T淋巴细胞病毒I型(HTLV-I)等信号通路。【结论】杜仲可能通过PTH、BGP、PPAR、ALP、RUNX2等靶标达到治疗骨质疏松的目的,其治疗骨质疏松的过程可能涉及PI3K-Akt、VEGF、AMPK、Notch等信号通路。展开更多
The degradation of nitrobenzene by ceramic-honeycomb catalyzed ozonation was investigated. The results showed that the presence of ceramic honeycombs significantly increased the oxidation rate of nitrobenzene by ozone...The degradation of nitrobenzene by ceramic-honeycomb catalyzed ozonation was investigated. The results showed that the presence of ceramic honeycombs significantly increased the oxidation rate of nitrobenzene by ozone compared to the case of ozone oxidation alone. In this paper, the effects of various factors on the catalytic oxidation were investigated, such as the amount of catalysts, the ozone dosage, the temperature, the pH value and the presence of tert-butanol. With the addition of tert-butanol the removal of nitrobenzene decreased sharply, which appeared to support that, the degradation of nitrobenzene by ozonation followed a radical type mechanism. The EPR experiments verified that higher nitrobenzene removal rate was attributed to more OH radicals generated in the catalyzed ozonation than ozonation alone.展开更多
The formation of thawed interlayer beneath embankment can result in embankment settlement in permafrost regions. Based on the data on ground temperatures and deformations beneath the embankment, observed in-situ along...The formation of thawed interlayer beneath embankment can result in embankment settlement in permafrost regions. Based on the data on ground temperatures and deformations beneath the embankment, observed in-situ along the QinghaiTibet Railway in permafrost regions from 2006 to2013, characteristics of the thawed interlayer beneath the embankment and its influence on the embankment settlement are studied. The results indicate that the thawed interlayer hardly forms beneath the natural field, and beneath the embankments from the Qinghai-Tibet Railway the thawed interlayer develops widely, and it can be refrozen totally in the regions with lower mean annual ground temperature, and developed further in the regions with higher mean annual ground temperature.The thawed interlayer is closely related to the embankment settlement. The ice content of permafrost underlying the thawed interlayer influences the settlement of embankment. The higher the ice content is, the larger the settlement is, and vice versa. The increase in thickness of thawed interlayer mainly results from the decline of artificial permafrost table in high-temperature permafrost regions.展开更多
文摘【目的】利用网络药理学的方法探讨杜仲治疗骨质疏松(OP)的可能作用机制。【方法】通过中药系统药理学数据库与分析平台(TCMSP)、文献检索获取杜仲的活性成分,利用反向分子对接服务器(DRAR-CPI)、GeneCards和在线人类孟德尔遗传(OMIM)数据库筛选杜仲的活性成分治疗骨质疏松的潜在作用靶点。应用Cytoscape软件构建杜仲的活性成分—作用靶点网络。应用String数据库和Cytoscape软件获得蛋白质相互作用网络。采用Systems Dock Web Site网络服务器与杜仲的活性成分进行分子对接。利用DAVID数据库对杜仲的作用靶点进行基因本体论(GO)分类富集分析和京都基因和基因组百科全书(KEGG)通路富集分析。【结果】从杜仲中筛选出活性成分12个,作用靶标35个。杜仲治疗骨质疏松主要涉及磷脂酰肌醇-3-激酶(PI3K)/丝苏氨酸蛋白激酶Akt、血管内皮生长因子(VEGF)、腺苷酸活化蛋白激酶(AMPK)、Notch、核因子kappaB(NF-κB)、叉头框蛋白(FoxO)、人嗜T淋巴细胞病毒I型(HTLV-I)等信号通路。【结论】杜仲可能通过PTH、BGP、PPAR、ALP、RUNX2等靶标达到治疗骨质疏松的目的,其治疗骨质疏松的过程可能涉及PI3K-Akt、VEGF、AMPK、Notch等信号通路。
基金The National Natural Science Foundation of China(No.50378028)
文摘The degradation of nitrobenzene by ceramic-honeycomb catalyzed ozonation was investigated. The results showed that the presence of ceramic honeycombs significantly increased the oxidation rate of nitrobenzene by ozone compared to the case of ozone oxidation alone. In this paper, the effects of various factors on the catalytic oxidation were investigated, such as the amount of catalysts, the ozone dosage, the temperature, the pH value and the presence of tert-butanol. With the addition of tert-butanol the removal of nitrobenzene decreased sharply, which appeared to support that, the degradation of nitrobenzene by ozonation followed a radical type mechanism. The EPR experiments verified that higher nitrobenzene removal rate was attributed to more OH radicals generated in the catalyzed ozonation than ozonation alone.
基金supported by the National Natural Science Foundation of China(Grant Nos.41571064,41630636 and 41471061)the Independent Research of the State Key Laboratory of Frozen Soil Engineering(SKLFSE-ZT-09)
文摘The formation of thawed interlayer beneath embankment can result in embankment settlement in permafrost regions. Based on the data on ground temperatures and deformations beneath the embankment, observed in-situ along the QinghaiTibet Railway in permafrost regions from 2006 to2013, characteristics of the thawed interlayer beneath the embankment and its influence on the embankment settlement are studied. The results indicate that the thawed interlayer hardly forms beneath the natural field, and beneath the embankments from the Qinghai-Tibet Railway the thawed interlayer develops widely, and it can be refrozen totally in the regions with lower mean annual ground temperature, and developed further in the regions with higher mean annual ground temperature.The thawed interlayer is closely related to the embankment settlement. The ice content of permafrost underlying the thawed interlayer influences the settlement of embankment. The higher the ice content is, the larger the settlement is, and vice versa. The increase in thickness of thawed interlayer mainly results from the decline of artificial permafrost table in high-temperature permafrost regions.
