Objective: To evaluate the effect of ginger (Zingiber officinale) aqueous extract, a natural herb, with antioxidant properties, on testicular toxicity and oxidative stress induced by the antithyroid drug carbimazole i...Objective: To evaluate the effect of ginger (Zingiber officinale) aqueous extract, a natural herb, with antioxidant properties, on testicular toxicity and oxidative stress induced by the antithyroid drug carbimazole in albino rats.Methods: Four groups of male albino rats were used. Group I served as control. Group II rats were treated with ginger aqueous extract (24 mg/mL). Group III rats were given orally carbimazole (1.35 mg/kg bw). Group IV rats were given carbimazole and ginger extract. Animals were sacrificed and their testes were removed and stained with H&E for histological examination. Sperms were collected from epididymis for detection of sperm head abnormalities. Immunohistochemical expression of PCNA and Bax was detected in the testes.MDA, CAT and GSH were measured in the sera.Results: Treating rats with carbimazole revealed significant alterations in the tissue of testis including decreased seminiferous epithelium height, decreased diameter of seminiferous tubule and changes in the spermatogenic layers arrangement. Intertubular hemorrhage and congested blood vessels were noted. An increase in sperm head abnormalities was recorded.Decreased cell proliferation was reflected by a decrease in PCNA expression, while the increase in apoptotic rate was accompanied with an increase in Bax expression. Oxidative stress was demonstrated by an increase in malondialdehyde and decrease in activity of catalase and glutathione. Combined treatment of carbimazole and aqueous ginger extract led to an improvement in histological, morphometrical, immunohistochemical changes and oxidative stress induced by carbimazole.Conclusions: The ameliorative effects of ginger extract could be due to its antioxidant properties.展开更多
Objective:To evaluate the effect of the antithyroid drug,carbimazole on liver of albino rats and the possible ameliorative role of selenium.Methods:Four groups of rats were used(n=10),Group 1 served as normal control,...Objective:To evaluate the effect of the antithyroid drug,carbimazole on liver of albino rats and the possible ameliorative role of selenium.Methods:Four groups of rats were used(n=10),Group 1 served as normal control,Group 2 was orally given sodium selenite(10μg/kg body weight)daily for 8 weeks,Group 3 was orally given carbimazole at a dose level of 1.35 mg/kg body weight,Group 4 was orally administered carbimazole and sodium selenite daily for 8 weeks.Rats in control and treated groups were sacrificed by cervical decapitation after 8 weeks of treatment,their livers were removed and stained with H&E for histological examinations.Alanine aminotransferase and aspartate aminotransferase were determined in the sera.Malondialdehyde and the antioxidant enzymes,catalase and superoxide dismutase were measured in the liver.Results:Cytoplasmic vacuolation of the hepatocytes,necrosis,leucocytic infiltrations,blood vessels congestion and fatty degeneration were observed in liver of carbimazole-treated animals.Carbimazole caused marked elevation in serum alanine aminotransferase and aspartate aminotransferase.It also caused an increase in malondialdehyde and depletion of the activity of the antioxidant enzymes,catalase and superoxide dismutase in the liver.Treating animals with carbimazole and selenium led to an improvement in both the histological and biochemical alterations induced by carbimazole.Moreover,selenium reduced the level of malondialdehyde and increased the activity of antioxidant enzymes,superoxide dismutase and catalase.Conclusions:It is concluded that the ameliorative effect of selenium against the hepatotoxicity of carbimazole is attributed to its antioxidant properties.展开更多
文摘Objective: To evaluate the effect of ginger (Zingiber officinale) aqueous extract, a natural herb, with antioxidant properties, on testicular toxicity and oxidative stress induced by the antithyroid drug carbimazole in albino rats.Methods: Four groups of male albino rats were used. Group I served as control. Group II rats were treated with ginger aqueous extract (24 mg/mL). Group III rats were given orally carbimazole (1.35 mg/kg bw). Group IV rats were given carbimazole and ginger extract. Animals were sacrificed and their testes were removed and stained with H&E for histological examination. Sperms were collected from epididymis for detection of sperm head abnormalities. Immunohistochemical expression of PCNA and Bax was detected in the testes.MDA, CAT and GSH were measured in the sera.Results: Treating rats with carbimazole revealed significant alterations in the tissue of testis including decreased seminiferous epithelium height, decreased diameter of seminiferous tubule and changes in the spermatogenic layers arrangement. Intertubular hemorrhage and congested blood vessels were noted. An increase in sperm head abnormalities was recorded.Decreased cell proliferation was reflected by a decrease in PCNA expression, while the increase in apoptotic rate was accompanied with an increase in Bax expression. Oxidative stress was demonstrated by an increase in malondialdehyde and decrease in activity of catalase and glutathione. Combined treatment of carbimazole and aqueous ginger extract led to an improvement in histological, morphometrical, immunohistochemical changes and oxidative stress induced by carbimazole.Conclusions: The ameliorative effects of ginger extract could be due to its antioxidant properties.
文摘Objective:To evaluate the effect of the antithyroid drug,carbimazole on liver of albino rats and the possible ameliorative role of selenium.Methods:Four groups of rats were used(n=10),Group 1 served as normal control,Group 2 was orally given sodium selenite(10μg/kg body weight)daily for 8 weeks,Group 3 was orally given carbimazole at a dose level of 1.35 mg/kg body weight,Group 4 was orally administered carbimazole and sodium selenite daily for 8 weeks.Rats in control and treated groups were sacrificed by cervical decapitation after 8 weeks of treatment,their livers were removed and stained with H&E for histological examinations.Alanine aminotransferase and aspartate aminotransferase were determined in the sera.Malondialdehyde and the antioxidant enzymes,catalase and superoxide dismutase were measured in the liver.Results:Cytoplasmic vacuolation of the hepatocytes,necrosis,leucocytic infiltrations,blood vessels congestion and fatty degeneration were observed in liver of carbimazole-treated animals.Carbimazole caused marked elevation in serum alanine aminotransferase and aspartate aminotransferase.It also caused an increase in malondialdehyde and depletion of the activity of the antioxidant enzymes,catalase and superoxide dismutase in the liver.Treating animals with carbimazole and selenium led to an improvement in both the histological and biochemical alterations induced by carbimazole.Moreover,selenium reduced the level of malondialdehyde and increased the activity of antioxidant enzymes,superoxide dismutase and catalase.Conclusions:It is concluded that the ameliorative effect of selenium against the hepatotoxicity of carbimazole is attributed to its antioxidant properties.
