In silico evaluation of kuwanon compounds as antiviral agents targeting H9N2 influenza virus

Background:The threat of avian influenza a subtype avian influenza A(H9N2)virus remains a significant concern,necessitating the exploration of novel antiviral agents.This study employs network pharmacology and computational analysis to investigate the potential of kuwanons,a natural compounds against H9N2 influenza virus.Methods:Leveraging comprehensive databases and bioinformatics tools,we elucidate the molecular mechanisms underlying Kuwanons pharmacological effects against H9N2 influenza virus.Network pharmacology identifies H9N2 influenza virus targets and compounds through integrated protein-protein interaction and Kyoto Encyclopedia of Genes and Genomes analyses.Molecular docking studies were performed to assess the binding affinities and structural interactions of Kuwanon analogues with key targets,shedding light on their potential inhibitory effects on viral replication and entry.Results:Compound-target network analysis revealed complex interactions(120 nodes,163 edges),with significant interactions and an average node degree of 2.72.Kyoto Encyclopedia of Genes and Genomes analysis revealed pathways such as Influenza A,Cytokine-cytokine receptor interaction pathway in H9N2 influenza virus.Molecular docking studies revealed that the binding free energy for the docked ligands ranged between-5.2 and-9.4 kcal/mol for the human interferon-beta crystal structure(IFNB1,Protein Data Bank:1AU1)and-5.4 and-9.6 kcal/mol for Interleukin-6(IL-6,PDB:4CNI).Conclusion:Our findings suggest that kuwanon exhibits promising antiviral activity against H9N2 influenza virus by targeting specific viral proteins,highlighting its potential as a natural therapeutic agent in combating avian influenza infections.

Sewage Sludge: An Important Biological Resource for Sustainable Agriculture and Its Environmental Implications

Intensive farming generally needs large addition of organic matter to maintain fertility and enhance crop yields. Sewage sludge/biosolids are by-products of municipal and industrial wastewater treatment and a rich source of organic nutrients. Sewage sludge having high content of organic matter, macro- and micro-nutrients, can be used as fertilizer/soil conditioner for food, vegetable crop, horticultural plants and pasture, which in most cases can be beneficially recycled. In the past sewage sludge was regarded as a waste product due to expected high level of contaminants such as pathogens, pollutants and synthetic materials discharged in sewer from homes and industries, which were often incinerated, dumped in occasion or land fill. As a result of rapidly increasing population, urbanization and industrialization, wastewater production and sewage sludge generation have increased manifold. Due to high cost of mineral fertilizers and escalating trends in their prices, there is an increasing trend of using sewage sludge in agriculture, especially under intensive cropping in arid and semi arid regions of the country. Therefore, application of sewage sludge to agricultural soils may be sustainable and economical due to nutrient cycling and disposal of sewage sludge. However, there may be a risk in use of sewage sludge due to potentially harmful contents present in the sludge such as heavy metals and pathogens. This paper, therefore, presents a review on various aspects of sewage sludge used in agriculture.

Effect of Tillage and Nitrogen on Wheat Production, Economics, and Soil Fertility in Rice-Wheat Cropping System

Conservation tillage and nitrogen may improve soil fertility, yield and income on sustainable basis. The aim of this study was to evaluate the impact of three tillage systems viz. zero (ZT), reduced (RT), and conventional tillage (CT) and five N rates (0, 80, 120, 160, and 200 kg·N·ha-1) on yield and yield components, soil organic matter (SOM), total soil N (TSN), and income of wheat (Triticum aestivum L.) grown after rice (Oryza sativa L.). Nitrogen rates significantly affected yield and yield attributing characters with highest values recorded at200 kg·N·ha-1. Mean maximum grain yield (6390 -6845 kg·ha-1), net benefit (1359 - 1460 US$ ha-1), and benefit cost ratio (5.6 - 5.7) could be achieved at 160 to200 kg·N·ha-1. The tillage systems produced similar yield and yield components except spikes m-2. The SOM and TSN were highest in ZT compared to other tillage systems. Though tillage × N interactions were not significant for most of the parameters under study, the overall effect of ZT with200 kg·N·ha-1 appeared to be most favorable compared to RT and CT. Zero tillage resulted in highest number of spikes m-2 (304.4), SOM (9.6 g·kg-1) and TSN (0.5 g·kg-1) with200 kg·N·ha-1. The results suggest that ZT with200 kg·N·ha-1 was optimum and sustainable strategy to achieve higher yield and income and also to improve SOM and TSN on silty clay soil.