The purpose of this work is to shed light on the effect of the pivot position on the surface pressure distribution over a 3D wing in different flight conditions.The study is intended to support the design and developm...The purpose of this work is to shed light on the effect of the pivot position on the surface pressure distribution over a 3D wing in different flight conditions.The study is intended to support the design and development of aerospace vehicles where stability analysis,performance optimization,and aircraft design are of primary importance.The following parameters are considered:Mach numbers(M)of 1.3,1.8,2.3,2.8,3.3,and 3.8,angle of incidence(θ)in the range from 5°to 25°,pivot position from h=0.2 to 1.The results of the CFD numerical simulations match available analytical data,thereby providing evidence for the reliability of the used approach.The findings provide valuable insights into the relationship between the surface pressure distribution,the Mach number and the angle of incidence.展开更多
The generation of stable clones for biomolecule production is a common but lengthy and labor-intensive process.For complex molecules,such as viruses or virus-like particles(VLPs),the timeline becomes even more cumbers...The generation of stable clones for biomolecule production is a common but lengthy and labor-intensive process.For complex molecules,such as viruses or virus-like particles(VLPs),the timeline becomes even more cumbersome.Thus,in the early stages of development,transient production methods serve as a reasonable alternative to stable clone construction.In this work,an investigation of a polyethylenimine-(PEI-)based transfection method for the transient production of Chikungunya(Chik)VLPs,a vaccine candidate molecule,was undertaken.This effort focuses on tracking cell population responses during transfection,understanding how process changes affect these responses,and monitoring patterns in cell performance over the culture duration.Plasmid labeling and VLP staining were employed to comprehensively track cells via flow cytometry and to draw correlations between plasmid DNA(pDNA)uptake and the resulting VLP expression.The method detected high transfection efficiency(≥97%)in all samples tested and demonstrated the capability to track kinetics of plasmid-cell binding.With varied transfection cell concentrations,the pDNA binding kinetics are altered and saturation binding is observed in the lowest cell concentration sample tested in less than 3 hours of incubation.Interestingly,in all samples,the flow cytometry analysis of relative pDNA amount versus VLP expression staining showed that cells which contained fewer pDNA complexes resulted in the highest levels of VLP stain.Finally,to determine the potential breadth of our observations,we compared daily expression patterns of ChikVLP with a reporter,monomeric GFP molecule.The similarities detected suggest the interpretations presented here to likely be more broadly informative and applicable to PEI-based transient production of additional biological products as well.展开更多
文摘The purpose of this work is to shed light on the effect of the pivot position on the surface pressure distribution over a 3D wing in different flight conditions.The study is intended to support the design and development of aerospace vehicles where stability analysis,performance optimization,and aircraft design are of primary importance.The following parameters are considered:Mach numbers(M)of 1.3,1.8,2.3,2.8,3.3,and 3.8,angle of incidence(θ)in the range from 5°to 25°,pivot position from h=0.2 to 1.The results of the CFD numerical simulations match available analytical data,thereby providing evidence for the reliability of the used approach.The findings provide valuable insights into the relationship between the surface pressure distribution,the Mach number and the angle of incidence.
基金This work was supported by the intramural research program of the Vaccine Research Center(VRC)National Institute of Allergy and Infectious Diseases(NIAID)National Insti-tutes of Health(NIH).
文摘The generation of stable clones for biomolecule production is a common but lengthy and labor-intensive process.For complex molecules,such as viruses or virus-like particles(VLPs),the timeline becomes even more cumbersome.Thus,in the early stages of development,transient production methods serve as a reasonable alternative to stable clone construction.In this work,an investigation of a polyethylenimine-(PEI-)based transfection method for the transient production of Chikungunya(Chik)VLPs,a vaccine candidate molecule,was undertaken.This effort focuses on tracking cell population responses during transfection,understanding how process changes affect these responses,and monitoring patterns in cell performance over the culture duration.Plasmid labeling and VLP staining were employed to comprehensively track cells via flow cytometry and to draw correlations between plasmid DNA(pDNA)uptake and the resulting VLP expression.The method detected high transfection efficiency(≥97%)in all samples tested and demonstrated the capability to track kinetics of plasmid-cell binding.With varied transfection cell concentrations,the pDNA binding kinetics are altered and saturation binding is observed in the lowest cell concentration sample tested in less than 3 hours of incubation.Interestingly,in all samples,the flow cytometry analysis of relative pDNA amount versus VLP expression staining showed that cells which contained fewer pDNA complexes resulted in the highest levels of VLP stain.Finally,to determine the potential breadth of our observations,we compared daily expression patterns of ChikVLP with a reporter,monomeric GFP molecule.The similarities detected suggest the interpretations presented here to likely be more broadly informative and applicable to PEI-based transient production of additional biological products as well.
