qGW11a/OsCAT8,encoding an amino acid permease,negatively regulates grain size and weight in rice

Grain size is a key factor influencing grain weight in rice.In this study,a chromosome segment substitution line(CSSL9-17)was identified,that exhibits a significant reduction in both grain size and weight compared to its donor parent 93-11.Further investigation identified two quantitative trait loci(QTL)on chromosome 11,designated qGW11a and qGW11b,which contribute to 1000-grain weight with an additive effect.LOC_Os11g05690,encoding the amino acid permease OsCAT8,is the target gene of qGW11a.Overexpression of OsCAT8 resulted in decreased grain weight,while OsCAT8 knockout mutants exhibited increased grain weight.The 287-bp located within the OsCAT8 promoter region of 93-11 negatively regulates its activity,which is subsequently correlated with an increase in grain size and weight.These results suggest that OsCAT8 functions as a negative regulator of grain size and grain weight in rice.

研究型规划设计的教学实践与思考——基于“城水耦合”视角

为改善传统城乡规划设计教学实践中研究与设计脱节问题,强化规划研究与规划设计形成有效合力,华南理工大学建筑学院教学团队结合“十三五”国家重点研发计划课题《城市新区水环境系统与规划设计优化技术》研究,以“城水耦合”视角开展研究型规划设计教学实践工作。截至目前,已完成两期城市总体规划、四期毕业设计的本科教学工作,并培养了多位博硕士研究生。

Chemical Fertilizer Reduction and High Yield Cultivation Technique for Peanut

For a long time,the amount of fertilizer applied to peanuts in China has been much higher than that of other main peanut producing countries.At the same time of increasing production,chemical fertilizers have also brought many adverse effects,which have brought potential threats to the sustainable development of peanut production.While continuously increasing the yield,reducing the amount of chemical fertilizer has become an urgent problem to be solved in peanut production of China.Based on the research results of our team,this paper appropriately absorbed the latest research progress of chemical fertilizer reduction in related fields,and established the cultivation technique for peanut fertilizer reduction and high yield under different cultivation modes,to realize the synchronization of fertilizer reduction and yield increase for peanut production.The technique includes two parts:common technology and different cultivation mode fertilization schemes.The former includes crop rotation,proper deep tillage,application of organic fertilizer,selection of nutrient-efficient varieties,topdressing of foliar fertilizer,etc.,the latter includes film mulching spring peanut,continuous cropping field,acidified soil,peanuts interplanting with wheat,and summer direct sowing,etc.This technique provides a technical support for the chemical fertilizer reduction of peanut production in China.

Structural reconstruction of Sn-based metal-organic frameworks for efficient electrochemical CO_(2) reduction to formate

MOF-based materials have been widely explored in electrochemical CO_(2)reduction reactions for the production of valuable chemicals.Understanding the reconstruction of those catalysts under working conditions is crucial for the identification of active sites and clarification of reaction mechanism.Herein,a series of six N coordinated Sn-based metal-organic frameworks(Sn-N6-MOFs)are newly developed for electrochemical CO_(2)reduction(CO_(2)RR).2%Sn-N6-MOF achieves the optimal catalytic performance with a formate Faradaic efficiency of~85%and a current density of 23 mA·cm^(-2) at-1.23 V vs.RHE.In-situ Raman results combined with ex-situ ^(119)Sn Mössbauer measurements reveal the structural reconstruction of Sn-N6-MOFs during CO_(2)RR,generating tin nanoclusters as the real active sites for CO_(2)electroreduction to HCOOH.

OsSPL18 controls grain weight and grain number in rice

Grain weight and grain number are two important traits directly determining grain yield in rice. To date,a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines.Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5?RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice.

Spatial Strategy for the Information Society:Rethinking Smart City

This study examines the topic of the information society from a spatial perspective. It reviews the basic impacts of information technology on urban space in terms of travel behavior, functional organization, and spatial image, and then puts forward the corresponding spatial planning strategy for the information society. Inf luenced by information technology, the concentration/ de-concentration of urban functional organization and the changes in travel demand have increased the complexity of the urban spatial structure, while the city image delivered by digital media promotes the blending of real space and virtual space. According to relevant researches, information society is considered an objective existence, whereas smart city can be perceived as a planning paradigm embodied with a promising vision. Therefore, spatial planning in the information society should be conducted from the two perspectives of both instrumental rationality and value rationality. On the basis of that, this study proposes that the construction of a smart city should foremost improve the spatial effi ciency with the help of information technology, and pay attention to the spatial probability during the process of urban development, so as to bestow the spaces of all levels the adaptability to respond to different scenarios; on the other hand, the spatial strategy of a smart city should return to people-oriented values and shape localized spaces, so as to cope with the challenges of global mobility and ultimately achieve sustainable regional development.