Disinfection Effects and Clinical Application of Sodium Dichloroisocyanurate Powder on Bacteria

With the continuous increase of large-scale pig farms,the disinfection of pig farm environment plays an extremely important part in the control and prevention of pig farm diseases in high-density breeding environment.As an important breeding place for pig farms,the farrowing house must be performed with scientific and standardized disinfection.In this paper,different disinfection modes were used to disinfect the empty farrowing houses of pig farms,and the total plate count,Escherichia coli,Staphylococcus aureus,and fungi were used as detection indicators to comprehensively evaluate the disinfection effect of the air microorganisms and the surface of the objects in the empty house after disinfection,with the aim to provide a basis for the establishment of a scientific pig farm environmental disinfection model.

Mutation Breeding of Lactobacillus and Its Application in Yoghurt

With the gradual improvement of the quality of people’s life, the disorder of lipid metabolism caused by high-fat diet has become the main reason that affects the health of our people. Hyperlipidemia, hypertension and hyperglycemia have the effect of causing a large number of serious complications, and are also the main factors of cardiovascular disease. Lactic acid bacteria can avoid hyperlipidemia by inhibiting the excessive growth of harmful intestinal bacteria, maintaining the balance of intestinal microecology, absorbing intestinal cholesterol and regulating lipid metabolism. Therefore, this paper focused on the mutation and breeding of Lactobacillus, and analyzed its application in yoghurt, aiming to provide reference for relevant researchers.

Role and Effects of Eucommia ulmoides Leaves in the Growth Process of Livestock and Poultry

Substitutes of feed antibiotics have been a key research topic in the new stage of animal husbandry.Chinese veterinary medicine refers to decoction pieces and their preparations processed from natural plants,animals,and minerals and used for animal disease prevention and improvement of animal production performance under the guidance of Chinese veterinary pharmacy theory.Eucommia ulmoides leaf extract has many active functional components such as chlorogenic acid,E.ulmoides polysaccharides and flavonoids,which have many biological properties such as antibacterial,antioxidant,immune-regulation,sugar ester-and bone metabolism-regulation effects.This paper explored active ingredients and biological properties of E.ulmoides leaf extract,as well as its role and effects in livestock and poultry breeding,providing a scientific basis for the use of E.ulmoides leaves in livestock and poultry breeding.

Study on Strengthening Animal Disease Prevention

With the in-depth development of China’s market economy,animal husbandry has become an important industry that affects China’s economic form.In order to effectively promote the healthy development of animal husbandry,attention should be paid to the prevention of animal diseases.This paper made a detailed analysis from the main problems encountered in the process of animal epidemic prevention work as an entry point,and put forward some effective measures for strengthening animal epidemic prevention work.

Bactericidal Effect and Clinical Application of 20% Sodium Dichloroisocyanurate Powder

[Objectives]This study was conducted to investigate the formulation stability of 20% sodium dichloroisocyanurate powder and its killing effect on common pathogenic bacteria in the animal breeding environment and to provide a scientific basis for veterinary clinical application. [Methods] The stability of 20% sodium dichloroisocyanurate powder was tested through an accelerated test and a long-term test. In the accelerated test, 20% sodium dichloroisocyanurate powder and sodium hypochlorite were stored in 37 ℃ constant temperature incubators for 90 d, and the available chlorine contents were compared. In the long-term test, 20% sodium dichloroisocyanurate powder was sealed and stored at room temperature, and its effective chlorine content and pH were measured regularly. In order to test its bactericidal effect in clinical use, 20% sodium dichloroisocyanurate powder, lime milk and 5% dilute glutaraldehyde solution were sprayed in groups to disinfect empty sheds in a pig farm, and the total colony count, Escherichia coli colony count, Staphylococcus aureus colony count and fungal colony count were used as testing indexes to comprehensively evaluate the disinfection effects on microorganisms in the air of the empty sheds and the surface of objects inside the sheds after disinfection. [Results] The 20% sodium dichloroisocyanurate powder had an available chlorine loss rate of 7.92% in the accelerated test, and the loss rates of available chlorine content and pH of 20% sodium dichloroisocyanurate powder in the long-term test(240 d) were 1.52% and 1.87% at room temperature, respectively. In the test to detect the sterilization effects on the pig farm, the total colony count, E. coli colony count, S. aureus colony count and fungal colony count of samples treated with 20% sodium dichloroisocyanurate powder were lower than those of other two groups(P<0.05), and the total colony count of samples disinfected with 20% sodium dichloroisocyanurate powder after cleaning the dirt was 20 times lower than that of samples disinfected directly without cleaning. In conclusion, 20% sodium dichloroisocyanurate powder was stable and effective in disinfection, and the presence of organic matter had little effect on the disinfection effect. [Conclusions] This study will provide important reference for the application of 20% sodium dichloroisocyanurate powder in veterinary clinic.

Determination of Doxycycline in Doxycycline Hydrochloride Soluble Powder under Different Chromatographic Conditions

[Objectives]This study was conducted to compare the accuracy and detection speed of high performance liquid chromatography for determining the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder under different chromatographic conditions, in order to improve the level of laboratory testing. [Methods] Four sets of experiments were designed through a cross-test method. The contents of doxycycline hydrochloride in the raw materials of doxycycline hydrochloride and doxycycline hydrochloride soluble powder were determined by changing the chromatographic column and mobile phase conditions, and the feasibility and practicability of the four methods were judged by comparing the detection results through data and chromatographic signal processing. [Results] The content of doxycycline hydrochloride in finished doxycycline hydrochloride soluble powder and the raw materials of doxycycline hydrochloride could be accurately determined under the four chromatographic conditions, of which the experimental group using Agilent C18 column and the mobile chromatography conditions of finished doxycycline hydrochloride soluble powder had the shortest retention time. [Conclusions] The high performance liquid chromatography method using Agilent C18 chromatographic column and mobile phase chromatographic conditions of finished doxycycline hydrochloride soluble powder products can quickly and accurately determine the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder.