Research and Practice on the Construction of Professional Cluster in Application-Oriented Local Undergraduate Universities:Taking Environ-mental Chemical Engineering Professional Cluster in Pingdingshan University as an Example

Based on the analysis of the importance of professional cluster construction by ecological theory,with the change of social demand for talents,this paper explores the practice of environmental chemical professional cluster construction in Pingdingshan University,including gradually perfecting teaching conditions and reforming teaching mode,breaking through the limitations of resources,integrating the boundaries of colleges and departments,integrating multiple resources,innovating systems and mechanisms,reconstructing professional clusters,decon-structing professional connotations,reorganizing curriculum systems,etc.,in order to better build the ecological chain network of education in application-oriented colleges and universities,realize the deep integration of industry and education,train future-oriented interdisciplinary applied talents of new engineering,and realize the construction of characteristic professional cluster in application-oriented colleges.

Understanding water conservation vs. prof ligation traits in vegetable legumes through a physio-transcriptomic-functional approach

Abstract Vegetable soybean and cowpea are related warm-season legumes showing contrasting leaf water use behaviors under similar root drought stresses,whose mechanisms are not well understood.Here we conducted an integrative phenomic-transcriptomic study on the two crops grown in a feedback irrigation system that enabled precise control of soil water contents.Continuous transpiration rate monitoring demonstrated that cowpea used water more conservatively under earlier soil drought stages,but tended to maintain higher transpiration under prolonged drought.Interestingly,we observed a soybean-specific transpiration rate increase accompanied by phase shift under moderate soil drought.Time-series transcriptomic analysis suggested a dehydration avoidance mechanism of cowpea at early soil drought stage,in which the VuHAI3 and VuTIP2;3 genes were suggested to be involved.Multifactorial gene clustering analysis revealed different responsiveness of genes to drought,time of day and their interactions between the two crops,which involved species-dependent regulation of the circadian clock genes.Gene network analysis identified two co-expression modules each associated with transpiration rate in cowpea and soybean,including a pair of negatively correlated modules between species.Module hub genes,including the ABA-degrading gene GmCYP707A4 and the trehalose-phosphatase/synthase gene VuTPS9 were identified.Intermodular network analysis revealed putative co-players of the hub genes.Transgenic analyses verified the role of VuTPS9 in regulating transpiration rate under osmotic stresses.These findings propose that species-specific transcriptomic reprograming in leaves of the two crops suffering similar soil drought was not only a result of the different drought resistance level,but a cause of it.

Research and Exploration on Ideological and Political Theories Teaching in the Course of Soil Science Experiment:Taking Pingdingshan University as an Example

Experimental course is an important link of ideological and political education in colleges and universities under the new situation,and plays an important role in the construction of ideological and political theories teaching in the course.Taking Pingdingshan University as an example,by introducing the real soil cases in Pingdingshan area,the ideological and political elements of the course are integrated into the teaching links of laboratory safety training,self-study before class,experimental teaching implementation,evaluation and improvement.By implementing the concept of"All-round Education"in experimental teaching,we are committed to realizing the comprehensive coverage of value shaping,knowledge inheritance and ability training,and explore and practice the reform of ideological and political theories teaching in the course of Soil Science Experiment.

Research Progress of miR164 and Target Gene NAC in Regulating Sugarcane Stress Tolerance

Studies have shown that miR164 is a highly conserved miRNA family between monocot and dicotyledonous plants,and it plays an important role in the growth and development of sugarcane organs and in response to stress.As the main target gene of miR164,NAC transcription factors are mainly regulated at the post-transcriptional level.MiR164:NAC module may play an important role in determining the adaptive response of sugarcane to stress.MiR164 has a regulatory effect on the expression of target gene NAC,and may be closely related to the resistance process of sugarcane to abiotic stress,which provides a reference for using miRNA to carry out sugarcane resistance molecular breeding.

Analysis of microRNA Expression Characteristics Related to Low Temperature Stress in Chewing Cane

[Objectives]To explore microRNA expression characteristics related to low temperature stress in chewing cane.[Methods]The research on miRNA under abiotic stress of sugarcane at home and abroad mainly focused on the types and regulation of miRNA under cold,heat,drought,high salt,and mechanical stress.However,there are few studies on miRNA under low temperature stress in chewing cane.The target genes of miR394 and miR825 in chewing cane were predicted and functionally analyzed by bioinformatics technology.[Results]The results showed that the target genes of miR394 and miR825 were mainly members of the WRKY transcription factor family,involved in plant growth,development and stress resistance.Real-time fluorescence quantitative PCR analyzed the expression characteristics of target miRNA in different tissues of chewing cane at different periods of low temperature stress.[Conclusions]The results showed that the expression of chewing cane miR394 and miR408 had temporal and spatial specificity and tissue specificity,both of which could respond to low temperature stress with significant differential expression.

Enhanced efficiency and stability of perovskite solar cells by 2D perovskite vapor-assisted interface optimization

Organic–inorganic perovskites solar cells(PSCs)have attracted great attention due to their rapid progress in power conversion efficiency(PCE).However,there is still an enormous challenge to achieve both high efficiency and stability devices as the decomposition of perovskite materials under humid and light conditions.Herein,we demonstrate that high efficiency and stability of PSCs can be obtained by the reaction of three-dimensional(3D)perovskite with 1,4-butanediamine iodide(BEAI2)vapor.The incorporation of BEAI2 intensively promotes the crystallization of perovskite film with large grain size(~500 nm).Further characterization reveals that the post-treatment perovskite film delivered low interface trap density with long carrier lifetime(>200 ns),long carrier diffusion length(>600 nm)and large carrier mobility(>1.5 cm^2 V-1S-1).Solar cells employing such post-treatment films demonstrated 19.58%PCE without hysteresis.Moreover,the post-treatment devices can retain over 90%original efficiencies stored under ambient atmospheric conditions and exhibit better stability under 85℃and continuous illumination as a two-dimensional(2D)perovskite thin layer is formed on the surface/or at the grain boundaries of 3D perovskite.This study offers an effective way to obtain PSCs with high efficiency and stability.

cDNA-SCoT Analysis of Differential Expressed Genes in Allium tuberosum Induced by Botrytis cinerea

[Objectives]To investigate the molecular mechanism of gene differential expression in A.tuberosum under Botrytis cinerea stress.[Methods]The leaf of A.tuberosum was inoculated with B.cinerea to induce resistance,then leaf samples were taken at 2,4,8,12 and 24 h,respectively,after inoculation,and used for cDNA-SCoT analysis to detect the gene differential expressions.[Results]More than 800 bands with length of 100-1800 bp were obtained using 60 SCoT primers.A total of 40 differentially expressed EST sequences were screened out,and 18 non-redundant ESTs with high quality were obtained by cluster analyses of the ESTs sequencing.The results of BlastN showed that the differential genes of A.tuberosum mainly related to disease defense,signal transduction,and protein metabolism.[Conclusions]Further analysis of gene function indicated that brassinosteroid biosynthesis-like proteins,NBS-LRR type disease resistance protein,jasmonic acid induced protein,abscisic stress ripening protein may be involved in the process of the incompatible interaction between the A.tuberosum and B.cinerea.

Phylogenetic Structure of Low Altitude Forest Communities in Baotianman Mountain

[Objectives]The paper was to reveal the phylogenetic structure of low altitude forest communities in Baotianman Mountain.[Methods]Ten 20 m×20 m plots were set at an altitude of 249-796 m in Baotianman Nature Reserve,in which 62 plant species were found.A phylogenetic tree was established on Phylomatic website,and the community related phylogenetic indexes at low altitude were calculated and analyzed,including net relatedness index(NRI),nearest taxon index(NTI),phylogenetic diversity Faith's PD index,phylogeneticβdiversity PhyloSor index and Dnn index.[Results]The NTI and NRI values of low altitude forest communities were generally greater than 0,showing phylogenetically clustered communities,and habitat filtration was the dominant factor in community construction.Combined with the species abundance matrix of plots,it was found that PD index had a significantly positive correlation with species richness.Phylogeneticβdiversity PhyloSor index was larger in the lower altitude area,which was exactly opposite to Dnn index.In other words,the more similar the community,the closer the distance between species,and the communities were phylogenetically clustered.[Conclusions]The study can provide a scientific basis for forest community management and restoration.

Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution

in this report, we compared transcriptomic differ- ences between a synthetic Populus section Tacamahaca triploid driven by second-division restitution and its parents using a high-throughput RNA-seq method. A total of 4,080 genes were differentially expressed between the high-growth vigor allotriploids （SDR-H） and their parents, and 719 genes were non-additively expressed in SDR-H. Differences in gene expres- sion between the allotriploid and male parent were more significant than those between the allotriploid and female parent, which may be caused by maternal effects. We observed 3,559 differentially expressed genes （DEGs） between the SDR-H and male parent. Notably, the genes were mainly involved in metabolic process, cell proliferation, DNA methylation, cell division, and meristem and developmental growth. Among the 1,056 DEGs between SDR-H and female parent, many genes were associated with metabolic process and carbon utilization. In addition, 1,789 DEGs between high- and low-growth vigorallotriploid were mainly associated with metabolic process, auxin poplar transport, and regulation of meristem growth. Our results indicated that the higher poplar ploidy level can generate extensive transcriptomic diversity compared with its parents. Overall, these results increased our understanding of the driving force for phenotypic variation and adaptation in allopolyploids driven by second-division restitution.