Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experime...Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experiments to understand the underlying salinity tolerance mechanism.This study investigated the effects of five salinity levels such as Control(CK)=0(without NaCl stress),S1=0.20%,S2=0.25%,S3=0.30%and S4=0.35%of NaCl concentrations of soil on wheat plants.The results showed that increased salinity concentration reduced the growth and yield of wheat cultivars(NM-14 and YM-23).However,YM-23(12.7%)yielded more than NM-14 at maximum salinity stress.The higher salinity(S4)increased the concentration of Na^(+)(4.3 to 5.8-fold)and P contents(2.5 to 2.2-fold),while reducing the average concentrations of K^(+),Cu,and K^(+)/Na^(+)ratio.The higher salinity(S4)reduced the spikelet length by 21.35%(followed by grain spike−1),and the starch content by 18.81%.In the YM-23 cultivar,higher salinity increased superoxide dismutase(SOD),total antioxidant capacity(TAC),and amylase.Compared to NM-14,induced expression of TaYUC2,6,and TaGA13ox,20ox genes were recorded in YM-23.Similarly,in YM-23 the stress-specific genes such as TaHSP70,90 were enhanced whereas,TaSOS1,2 were suppressed.Overall,our study revealed that salt tolerant cultivars modulate hormonal and antioxidant activities,thus maintaining high growth.展开更多
In order to enhance the degrading protein capability of purple non-sulfur bacteria(PNSB),an effective strain,L2,was used to co-culture with Rhodobacter sphaeroides ATCC17023.The effects of added strain on protein remo...In order to enhance the degrading protein capability of purple non-sulfur bacteria(PNSB),an effective strain,L2,was used to co-culture with Rhodobacter sphaeroides ATCC17023.The effects of added strain on protein removal of R.sphaeroides were investigated.Results showed that strain L2,being identified as Bacillus thuringiensis/cereus,had a high potential for producing protease with a production of 295 U/m L.The optimal B.thuringiensis/cereus(40 μL) could significantly increase protein degradation of R.sphaeroides.Protein removal and biomass production were improved by 483% and 67%,respectively.R.sphaeroides/total biomass production was more than 95%.Theoretical analysis revealed that R.sphaeroides syntrophically interacted with B.thuringiensis/cereus.Protein degradation of B.thuringiensis/cereus provided small molecule substrates(VFAs) for R.sphaeroides growth and cells materials synthesis.展开更多
In the automobile painting workshop,the oven will discharge harmful exhaust gas,the exhaust gas can be reused through the TNV system,the natural gas can meet the emission standard to the atmosphere after burning,and t...In the automobile painting workshop,the oven will discharge harmful exhaust gas,the exhaust gas can be reused through the TNV system,the natural gas can meet the emission standard to the atmosphere after burning,and the high temperature gas discharged TNV the system can carry considerable heat.Utilization can effectively improve the economic benefits of the factory.At present,the more mature scheme is to heat the high temperature exhaust gas through the heat exchanger,which can reduce the steam consumption of the factory.Based on the analysis of the comprehensive energy saving content of waste heat utilization of RTO flue gas,this paper hopes to provide some reference and reference for readers.展开更多
With the increasingly serious environmental problems,China has adopted more and more strict industrial production emission policies.Haze pollutes the weather and limits the production of polluting enterprises have ser...With the increasingly serious environmental problems,China has adopted more and more strict industrial production emission policies.Haze pollutes the weather and limits the production of polluting enterprises have seriously affected the normal production process of the factory.The pollutants discharged in automobile manufacturing process are increasing,and the environmental pollution caused by automobile coating needs to be solved urgently.Because a large amount of solvent-based paint is used in the production process,the pollution caused by volatile organic compounds is difficult to avoid.RCO is a new technology in the field of automobile coating waste gas treatment.Application of RCO in automobile coating waste gas treatment is studied.展开更多
With the continuous development of our country's economy,the demand for automobile is higher and higher after people's living standard is improving day by day.In just 40 years of reform and opening up,China...With the continuous development of our country's economy,the demand for automobile is higher and higher after people's living standard is improving day by day.In just 40 years of reform and opening up,China's automobile production and per capita possession have made a qualitative leap and a breakthrough in quantity.Greatly promoted the automobile industry's great development.展开更多
Developing advanced hydrogen storage materials with high capacity and efficient reversibility is a crucial aspect for utilizing hydrogen source as a promising alternate to fossil fuels.In this paper,we have systematic...Developing advanced hydrogen storage materials with high capacity and efficient reversibility is a crucial aspect for utilizing hydrogen source as a promising alternate to fossil fuels.In this paper,we have systematically investigated the hydrogen storage properties of neutral and negatively charged C_(9)N_(4) monolayer based on density functional theory(DFT).Our foundings indicate that injecting additional electrons into the adsorbent significantly boosts the adsorption capacity of C_(9)N_(4) monolayer to H2 molecules.The gravimetric density of negatively charged C_(9)N_(4) monolayer can reach up to 10.80 wt% when fully covered with hydrogen.Unlike other hydrogen storage methods,the storage and release processes happen automatically upon introducing or removing extra electrons.Moreover,these operations can be easily adjusted through activating or deactivating the charging voltage.As a result,the method is easily reversible and has tunable kinetics without requiring particular activators.Significantly,C_(9)N_(4) is proved to be a suitable candidate for efficient electron injection/release due to its well electrical conductivity.Our work can serve as a valuable guide in the quest for a novel category of materials for hydrogen storage with high capacity.展开更多
Natural extracellular vesicles(EVs)play important roles in many life processes such as in the intermolecular transfer of substances and genetic information exchanges.Investigating the origins and working mechanisms of...Natural extracellular vesicles(EVs)play important roles in many life processes such as in the intermolecular transfer of substances and genetic information exchanges.Investigating the origins and working mechanisms of natural EVs may provide an understanding of life activities,especially regarding the occurrence and development of diseases.Additionally,due to their vesicular structure,EVs(in small molecules,nucleic acids,proteins,etc.)could act as efficient drug-delivery carriers.Herein,we describe the sources and biological functions of various EVs,summarize the roles of EVs in disease diagnosis and treatment,and review the application of EVs as drug-delivery carriers.We also assess the challenges and perspectives of EVs in biomedical applications.展开更多
It is of great significance to design and synthesize highly efficient n-type organic small molecules as electron-transporting layer(ETL) materials to achieve high performance planar perovskite solar cells(PVKSCs) with...It is of great significance to design and synthesize highly efficient n-type organic small molecules as electron-transporting layer(ETL) materials to achieve high performance planar perovskite solar cells(PVKSCs) with lowtemperature solution-processing. In this article, acenaphthylene-imide based small molecules AI1, AI2 and AI3 were developed with lower-lying LUMO energy levels and were further applied as non-fullerene ETL materials for solutionprocessed PVKSCs. When TiO2/AIX bilayer was used as ETL,an average power conversion efficiency(PCE) of 15.0% was achieved for TiO2/AI1 based PVKSCs with conventional configuration, while pristine TiO2 based devices presented a PCE of 11.7% only. All the results demonstrate that acenaphthylene-imide derivatives could be promising ETL materials to accomplish low-temperature solution-processed high performance PVKSCs.展开更多
Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimula...Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimulation could significantly enhance the R. sphaeroides biomass production and carotenoid yield. The optimal biostimulant proportion was 40 μL(about 6.4 × 10^5CFU). Through the use of biostimulation, chemical oxygen demand removal, R. sphaeroides biomass production, carotenoid concentration, and carotenoid yield were improved by 178%, 67%, 214%, and 70%, respectively. Theoretical analysis revealed that there were two possible reasons for such increases. One was that biostimulation enhanced the R. sphaeroides wastewater treatment efficiency. The other was that biostimulation significantly decreased the peroxidase activity in R. sphaeroides. The results showed that the highest peroxidase activity dropped by 87% and the induction ratio of the RSP_3419 gene was 3.1 with the addition of biostimulant. The enhanced carotenoid yield in R. sphaeroides could thus be explained by a decrease in peroxidase activity.展开更多
Dear Editor,Pathogenic microorganisms carrying antibiotic resistance genes(ARGs)pose serious challenge to human health worldwide.The major reservoir of ARGs is believed to be the environment(Cao et al.,2020).Some ARGs...Dear Editor,Pathogenic microorganisms carrying antibiotic resistance genes(ARGs)pose serious challenge to human health worldwide.The major reservoir of ARGs is believed to be the environment(Cao et al.,2020).Some ARGs can be transmitted from the environment into pathogens via horizontal gene transfer(Forsberg et al.,2012).展开更多
This study aimed to increase bacterial growth and 5-aminolevulinic acid(ALA) biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion( Fe2+ ). Results demonstrated that Fe2+ eff...This study aimed to increase bacterial growth and 5-aminolevulinic acid(ALA) biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion( Fe2+ ). Results demonstrated that Fe2+ effectively enhanced the biomass production and ALA yield of R. sphaeroides. Moreover, the optimal Fe2+ dosage was found to be 400 μmol/L, which was associated with the highest biomass of 4015.3 mg/L and maximum ALA yield of 15.9 mg/g-dry cell weight(mg/g-DCW). Mechanism analysis revealed that Fe2+ vastly improved Adenosine Triphosphate(ATP) production by up-regulating the nif gene expression, and increasing ATP enhanced the biomass and ALA yield by supplying energy for bacterial growth and ALA biosynthesis, respectively. Correlation analysis showed that the ALA and ATP yields had positive relation with nifA and nifU gene expression. In addition, the nifA and nifU gene expression displayed high consistency of co-transcription at the optimal Fe2+ dosage.展开更多
基金the National Natural Science Foundation of China(32101817)Jiangsu Agriculture Science and this work was funded by the National Natural Science Foundation of China(32101817)+3 种基金Jiangsu Agriculture Science and Technology Innovation Fund(CX(21)3111)the Natural Science Foundation of the Jiangsu Higher Education Institutions(21KJD210001)the Scientific and Technological Innovation Fund of Carbon Emissions Peak and Neutrality of Jiangsu Provincial Department of Science and Technology(BE2022304)the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)for their financial support.
文摘Understanding physiological responses in saline agriculture may facilitate wheat breeding programs.Based on a screening test,the Ningmai-14(NM-14)and Yangmai-23(YM-23)wheat cultivars were selected for further experiments to understand the underlying salinity tolerance mechanism.This study investigated the effects of five salinity levels such as Control(CK)=0(without NaCl stress),S1=0.20%,S2=0.25%,S3=0.30%and S4=0.35%of NaCl concentrations of soil on wheat plants.The results showed that increased salinity concentration reduced the growth and yield of wheat cultivars(NM-14 and YM-23).However,YM-23(12.7%)yielded more than NM-14 at maximum salinity stress.The higher salinity(S4)increased the concentration of Na^(+)(4.3 to 5.8-fold)and P contents(2.5 to 2.2-fold),while reducing the average concentrations of K^(+),Cu,and K^(+)/Na^(+)ratio.The higher salinity(S4)reduced the spikelet length by 21.35%(followed by grain spike−1),and the starch content by 18.81%.In the YM-23 cultivar,higher salinity increased superoxide dismutase(SOD),total antioxidant capacity(TAC),and amylase.Compared to NM-14,induced expression of TaYUC2,6,and TaGA13ox,20ox genes were recorded in YM-23.Similarly,in YM-23 the stress-specific genes such as TaHSP70,90 were enhanced whereas,TaSOS1,2 were suppressed.Overall,our study revealed that salt tolerant cultivars modulate hormonal and antioxidant activities,thus maintaining high growth.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51278489)
文摘In order to enhance the degrading protein capability of purple non-sulfur bacteria(PNSB),an effective strain,L2,was used to co-culture with Rhodobacter sphaeroides ATCC17023.The effects of added strain on protein removal of R.sphaeroides were investigated.Results showed that strain L2,being identified as Bacillus thuringiensis/cereus,had a high potential for producing protease with a production of 295 U/m L.The optimal B.thuringiensis/cereus(40 μL) could significantly increase protein degradation of R.sphaeroides.Protein removal and biomass production were improved by 483% and 67%,respectively.R.sphaeroides/total biomass production was more than 95%.Theoretical analysis revealed that R.sphaeroides syntrophically interacted with B.thuringiensis/cereus.Protein degradation of B.thuringiensis/cereus provided small molecule substrates(VFAs) for R.sphaeroides growth and cells materials synthesis.
文摘In the automobile painting workshop,the oven will discharge harmful exhaust gas,the exhaust gas can be reused through the TNV system,the natural gas can meet the emission standard to the atmosphere after burning,and the high temperature gas discharged TNV the system can carry considerable heat.Utilization can effectively improve the economic benefits of the factory.At present,the more mature scheme is to heat the high temperature exhaust gas through the heat exchanger,which can reduce the steam consumption of the factory.Based on the analysis of the comprehensive energy saving content of waste heat utilization of RTO flue gas,this paper hopes to provide some reference and reference for readers.
文摘With the increasingly serious environmental problems,China has adopted more and more strict industrial production emission policies.Haze pollutes the weather and limits the production of polluting enterprises have seriously affected the normal production process of the factory.The pollutants discharged in automobile manufacturing process are increasing,and the environmental pollution caused by automobile coating needs to be solved urgently.Because a large amount of solvent-based paint is used in the production process,the pollution caused by volatile organic compounds is difficult to avoid.RCO is a new technology in the field of automobile coating waste gas treatment.Application of RCO in automobile coating waste gas treatment is studied.
文摘With the continuous development of our country's economy,the demand for automobile is higher and higher after people's living standard is improving day by day.In just 40 years of reform and opening up,China's automobile production and per capita possession have made a qualitative leap and a breakthrough in quantity.Greatly promoted the automobile industry's great development.
基金The work was founded by Henan Scientific Research Fund for Returned Scholars,the Young Scientist Project of Henan Province(Grant No.225200810103)the Program for Science&Technology Innovation Talents in Universities of Henan Province(Grant No.24HASTIT013)+3 种基金Henan College Key Research Project(Grant No.24A430002)the Natural Science Foundation of Henan Province(Grant No.232300420128)the Scientific Research Innovation Team Project of Anyang Normal University(Grant No.2023AYSYKYCXTD04)the College Students Innovation Fund of Anyang Normal University(Grant No.202310479077).
文摘Developing advanced hydrogen storage materials with high capacity and efficient reversibility is a crucial aspect for utilizing hydrogen source as a promising alternate to fossil fuels.In this paper,we have systematically investigated the hydrogen storage properties of neutral and negatively charged C_(9)N_(4) monolayer based on density functional theory(DFT).Our foundings indicate that injecting additional electrons into the adsorbent significantly boosts the adsorption capacity of C_(9)N_(4) monolayer to H2 molecules.The gravimetric density of negatively charged C_(9)N_(4) monolayer can reach up to 10.80 wt% when fully covered with hydrogen.Unlike other hydrogen storage methods,the storage and release processes happen automatically upon introducing or removing extra electrons.Moreover,these operations can be easily adjusted through activating or deactivating the charging voltage.As a result,the method is easily reversible and has tunable kinetics without requiring particular activators.Significantly,C_(9)N_(4) is proved to be a suitable candidate for efficient electron injection/release due to its well electrical conductivity.Our work can serve as a valuable guide in the quest for a novel category of materials for hydrogen storage with high capacity.
基金funded by the National Natural Science Foundation of China(31901007,81630023,81970852,and 82000962)CAMS Innovation Fund for Medical Sciences,China(2018-I2M-3-006 and 2019-I2M-5-022,China)+6 种基金China Postdoctoral Science Foundation(2020T130006ZX)the Open Project Fund provided by Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS(NSKF202019,China)the State Key Laboratory Special Fund 2060204the National Key R&D Program of China(2016YFC0905200)the program for the Changjiang scholars and innovative research team(IRT13082,China)the Beijing Bai-Qian-Wan talent project(2019A32,China)the Public Welfare Development and Reform Pilot Project(2019-10,China)
文摘Natural extracellular vesicles(EVs)play important roles in many life processes such as in the intermolecular transfer of substances and genetic information exchanges.Investigating the origins and working mechanisms of natural EVs may provide an understanding of life activities,especially regarding the occurrence and development of diseases.Additionally,due to their vesicular structure,EVs(in small molecules,nucleic acids,proteins,etc.)could act as efficient drug-delivery carriers.Herein,we describe the sources and biological functions of various EVs,summarize the roles of EVs in disease diagnosis and treatment,and review the application of EVs as drug-delivery carriers.We also assess the challenges and perspectives of EVs in biomedical applications.
基金supported by the National Natural Science Foundation of China (61604071, 61525402, 61604119, 61704131 and 61775095)Natural Science Foundation of Jiangsu Province (BK20161012)+1 种基金Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJZZ16_0139)SICAM Scholarship (38600001)
文摘It is of great significance to design and synthesize highly efficient n-type organic small molecules as electron-transporting layer(ETL) materials to achieve high performance planar perovskite solar cells(PVKSCs) with lowtemperature solution-processing. In this article, acenaphthylene-imide based small molecules AI1, AI2 and AI3 were developed with lower-lying LUMO energy levels and were further applied as non-fullerene ETL materials for solutionprocessed PVKSCs. When TiO2/AIX bilayer was used as ETL,an average power conversion efficiency(PCE) of 15.0% was achieved for TiO2/AI1 based PVKSCs with conventional configuration, while pristine TiO2 based devices presented a PCE of 11.7% only. All the results demonstrate that acenaphthylene-imide derivatives could be promising ETL materials to accomplish low-temperature solution-processed high performance PVKSCs.
基金supported by the National Natural Science Foundation of China (No. 51278489)
文摘Bacillus thuringiensis/cereus L2 was added as a biostimulant to enhance the biomass accumulation and carotenoid yield of Rhodobacter sphaeroides using wastewater as the culturing medium. Results showed that biostimulation could significantly enhance the R. sphaeroides biomass production and carotenoid yield. The optimal biostimulant proportion was 40 μL(about 6.4 × 10^5CFU). Through the use of biostimulation, chemical oxygen demand removal, R. sphaeroides biomass production, carotenoid concentration, and carotenoid yield were improved by 178%, 67%, 214%, and 70%, respectively. Theoretical analysis revealed that there were two possible reasons for such increases. One was that biostimulation enhanced the R. sphaeroides wastewater treatment efficiency. The other was that biostimulation significantly decreased the peroxidase activity in R. sphaeroides. The results showed that the highest peroxidase activity dropped by 87% and the induction ratio of the RSP_3419 gene was 3.1 with the addition of biostimulant. The enhanced carotenoid yield in R. sphaeroides could thus be explained by a decrease in peroxidase activity.
基金supported by the Consulting Project about Bacterial Resistance from CASthe Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29010202)the National Key Research and Development Program of China(2016YFC1200804)。
文摘Dear Editor,Pathogenic microorganisms carrying antibiotic resistance genes(ARGs)pose serious challenge to human health worldwide.The major reservoir of ARGs is believed to be the environment(Cao et al.,2020).Some ARGs can be transmitted from the environment into pathogens via horizontal gene transfer(Forsberg et al.,2012).
基金supported by the National Natural Science Foundation of China(No.51708214)the High-level Personnel Research Startup Project of North China University of Water Resources and Electric Power(No.40550)the Treatment Technology Integration and Demonstration for Domestic Sewage of Typical Villages and Towns in Henan Province(No.161100310700)
文摘This study aimed to increase bacterial growth and 5-aminolevulinic acid(ALA) biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion( Fe2+ ). Results demonstrated that Fe2+ effectively enhanced the biomass production and ALA yield of R. sphaeroides. Moreover, the optimal Fe2+ dosage was found to be 400 μmol/L, which was associated with the highest biomass of 4015.3 mg/L and maximum ALA yield of 15.9 mg/g-dry cell weight(mg/g-DCW). Mechanism analysis revealed that Fe2+ vastly improved Adenosine Triphosphate(ATP) production by up-regulating the nif gene expression, and increasing ATP enhanced the biomass and ALA yield by supplying energy for bacterial growth and ALA biosynthesis, respectively. Correlation analysis showed that the ALA and ATP yields had positive relation with nifA and nifU gene expression. In addition, the nifA and nifU gene expression displayed high consistency of co-transcription at the optimal Fe2+ dosage.